A new sub-sampling method for analysis of air samples collected with the Andersen single-stage sampler

A new sub-sampling method for analysis of air samples collected with the Andersen single-stage sampler

A protocol for bioaerosol collection was developed that provides not only accurate predictions of fungal concentration, but also improves species recovery. Random transfer of a subset of 50 of the 400 impaction points from Andersen single-stage bioaerosol sampling plates results in subcultures that...

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Veröffentlicht in:Aerobiologia 2006-09, Vol.22 (3), p.177-184
Hauptverfasser: Catranis, Catharine M., Anagnost, Susan E., Zhang, Lianjun, Zhou, Shuang, Fernando, Analie, Morey, Shannon, Wang, C. J. K.
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container_end_page 184
container_issue 3
container_start_page 177
container_title Aerobiologia
container_volume 22
creator Catranis, Catharine M.
Anagnost, Susan E.
Zhang, Lianjun
Zhou, Shuang
Fernando, Analie
Morey, Shannon
Wang, C. J. K.
description A protocol for bioaerosol collection was developed that provides not only accurate predictions of fungal concentration, but also improves species recovery. Random transfer of a subset of 50 of the 400 impaction points from Andersen single-stage bioaerosol sampling plates results in subcultures that are accurate predictors of fungal concentration (CFU/m super(3)), when compared to duplicate untouched Andersen plates. A linear regression model was developed to estimate CFU/m super(3) from the colonies counted on the Random-50 plates. The random transfer to five plates ('Random-50' plates), allows large numbers of fungi to be recovered and identified, including slow-growing fungi that otherwise would be masked by fast-growing fungi.
doi_str_mv 10.1007/s10453-006-9030-2
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title A new sub-sampling method for analysis of air samples collected with the Andersen single-stage sampler
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