Molecular Identification of Aeromonas and Coliform Bacteria Isolated on m Endo Media from Lake Erie Waters
Bacteria from recreational waters collected from two Lake Erie beaches in Dunkirk, New York were plated onto m Endo LES media. The 16S rRNA gene was then amplified from coliform and non-coliform bacteria using the polymerase chain reaction. The PCR products were characterized by restriction fragment...
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| Veröffentlicht in: | World journal of microbiology & biotechnology 2006, Vol.22 (1), p.29-33 |
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| creator | Dickerman, Benjamin Metzger, James Lee, W. Theodore |
| description | Bacteria from recreational waters collected from two Lake Erie beaches in Dunkirk, New York were plated onto m Endo LES media. The 16S rRNA gene was then amplified from coliform and non-coliform bacteria using the polymerase chain reaction. The PCR products were characterized by restriction fragment length polymorphism (RFLP) analysis. A total of 8 RFLP groups were identified from the analysis of 920 samples and selected PCR products from each group were sequenced. The DNA sequence analysis indicated that more than half of the bacteria identified as coliforms on the m Endo plates belonged to the genus Aeromonas from the family Aeromonadaceae. Most of the remaining coliforms were from the Enterobacteriaceae. The data indicate that m Endo agar plates allow the growth of non-coliform bacteria, especially Aeromonas species. |
| doi_str_mv | 10.1007/s11274-005-4022-x |
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Theodore</creator><creatorcontrib>Dickerman, Benjamin ; Metzger, James ; Lee, W. Theodore</creatorcontrib><description>Bacteria from recreational waters collected from two Lake Erie beaches in Dunkirk, New York were plated onto m Endo LES media. The 16S rRNA gene was then amplified from coliform and non-coliform bacteria using the polymerase chain reaction. The PCR products were characterized by restriction fragment length polymorphism (RFLP) analysis. A total of 8 RFLP groups were identified from the analysis of 920 samples and selected PCR products from each group were sequenced. The DNA sequence analysis indicated that more than half of the bacteria identified as coliforms on the m Endo plates belonged to the genus Aeromonas from the family Aeromonadaceae. Most of the remaining coliforms were from the Enterobacteriaceae. The data indicate that m Endo agar plates allow the growth of non-coliform bacteria, especially Aeromonas species.</description><identifier>ISSN: 0959-3993</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1007/s11274-005-4022-x</identifier><language>eng</language><publisher>Dordrecht: Kluwer Academic Publishers</publisher><subject>Aeromonas ; agar ; Bacteria ; beaches ; Biological and medical sciences ; Biotechnology ; coliform bacteria ; DNA ; Enterobacteriaceae ; Fundamental and applied biological sciences. 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Theodore</creatorcontrib><title>Molecular Identification of Aeromonas and Coliform Bacteria Isolated on m Endo Media from Lake Erie Waters</title><title>World journal of microbiology & biotechnology</title><description>Bacteria from recreational waters collected from two Lake Erie beaches in Dunkirk, New York were plated onto m Endo LES media. The 16S rRNA gene was then amplified from coliform and non-coliform bacteria using the polymerase chain reaction. The PCR products were characterized by restriction fragment length polymorphism (RFLP) analysis. A total of 8 RFLP groups were identified from the analysis of 920 samples and selected PCR products from each group were sequenced. The DNA sequence analysis indicated that more than half of the bacteria identified as coliforms on the m Endo plates belonged to the genus Aeromonas from the family Aeromonadaceae. Most of the remaining coliforms were from the Enterobacteriaceae. The data indicate that m Endo agar plates allow the growth of non-coliform bacteria, especially Aeromonas species.</description><subject>Aeromonas</subject><subject>agar</subject><subject>Bacteria</subject><subject>beaches</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>coliform bacteria</subject><subject>DNA</subject><subject>Enterobacteriaceae</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Lakes</subject><subject>Microbiology</subject><subject>nucleotide sequences</subject><subject>polymerase chain reaction</subject><subject>Recreational waters</subject><subject>restriction fragment length polymorphism</subject><subject>RFLP</subject><subject>ribosomal RNA</subject><subject>sequence analysis</subject><issn>0959-3993</issn><issn>1573-0972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkU9r3DAQxUVoIdttPkBOEYX25kSjP5Z9TJdNsrChhybkKMayFLS1rVTyQvLtq2UDhVxmDvN7j5k3hJwDuwTG9FUG4FpWjKlKMs6r1xOyAKVFxVrNP5EFa1VbibYVp-RLzjvGiqoVC7K7j4Oz-wET3fRumoMPFucQJxo9vXYpjnHCTHHq6SoOwcc00p9oZ5cC0k2OA86upwUf6XrqI713fRn4oqNb_OPoOgVHnwqU8lfy2eOQ3dl7X5KHm_XD6q7a_rrdrK63lRVKz1XTQWctZ6Cb3jLLUfK6Fg5lp0rthbMMHKiGW6mtbjsJums8cIkth9qKJflxtH1J8e_e5dmMIVs3DDi5uM-GM81BFMsl-fYB3MV9mspqpmnkAQJeIDhCNsWck_PmJYUR05sBZg7Jm2PypiRvDsmb16L5_m6M2eLgE0425P9CrRolFRTu4sh5jAafU2Eef5fDa1beI0UN4h95i4v7</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>Dickerman, Benjamin</creator><creator>Metzger, James</creator><creator>Lee, W. 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Theodore</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-8b1bcc20178dc0c2a42663ea4b53ead3ec01e1582c47c79b417b8f124a9216c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aeromonas</topic><topic>agar</topic><topic>Bacteria</topic><topic>beaches</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>coliform bacteria</topic><topic>DNA</topic><topic>Enterobacteriaceae</topic><topic>Fundamental and applied biological sciences. 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The DNA sequence analysis indicated that more than half of the bacteria identified as coliforms on the m Endo plates belonged to the genus Aeromonas from the family Aeromonadaceae. Most of the remaining coliforms were from the Enterobacteriaceae. The data indicate that m Endo agar plates allow the growth of non-coliform bacteria, especially Aeromonas species.</abstract><cop>Dordrecht</cop><pub>Kluwer Academic Publishers</pub><doi>10.1007/s11274-005-4022-x</doi><tpages>5</tpages></addata></record> |
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| subjects | Aeromonas agar Bacteria beaches Biological and medical sciences Biotechnology coliform bacteria DNA Enterobacteriaceae Fundamental and applied biological sciences. Psychology genes Lakes Microbiology nucleotide sequences polymerase chain reaction Recreational waters restriction fragment length polymorphism RFLP ribosomal RNA sequence analysis |
| title | Molecular Identification of Aeromonas and Coliform Bacteria Isolated on m Endo Media from Lake Erie Waters |
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