An optimized controlled rate slow cooling protocol for bovine ovarian tissue cryopreservation by means of X-ray computed tomography
Cryopreservation and subsequent transplantation of ovarian tissue is the only option to preserve fertility in certain patients facing gonadotoxic treatment. So far, cryopreservation of ovarian tissue has been carried out mostly by a controlled rate slow cooling process, typically known as slow freez...
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| Veröffentlicht in: | Theriogenology 2018-10, Vol.119, p.183-188 |
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| creator | Corral, Ariadna Balcerzyk, Marcin Gallardo, Miguel Amorim, Christiani A. Parrado-Gallego, Ángel Risco, Ramón |
| description | Cryopreservation and subsequent transplantation of ovarian tissue is the only option to preserve fertility in certain patients facing gonadotoxic treatment. So far, cryopreservation of ovarian tissue has been carried out mostly by a controlled rate slow cooling process, typically known as slow freezing. Even though there are still some concerns about the iatrogenic damage on the follicle population, this technique has been used in the more than 100 live births reported to date. It is well known that the control of the cryoprotectant loading in the tissue is crucial to in a cryopreservation procedure. We have used the technology of X-ray computed tomography to assess the concentration and distribution of dimethyl sulfoxide (one of the cryoprotectants most used in fertility preservation) inside pieces of bovine ovarian tissue after its cryopreservation. The low voltage used in our device (75 kV) and the high electronic density of this cryoprotectant makes the X-ray attenuation proportional to its concentration. By assessing and comparing the permeation and homogeneity of the cryoprotectant inside ovarian tissue fragments subjected to a controlled rate slow cooling process, we have characterized the effect of variations in the main parameters involved in the process, with the goal of achieving an optimized protocol with higher permeation of the cryoprotectant in the tissue. The most promissory results were obtained by increasing the initial concentration of dimethyl sulfoxide in the vehicle solution from 10 to 20%v/v.
•Ovarian tissue cryopreservation as fertility preservation options for cancer patients.•Monitoring of cryopreservation procedures by X-ray Computed Tomography.•Understanding and optimization of controlled rate slow cooling protocols for tissue cryopreservation.•Dimethyl sulfoxide concentration proportional to X ray attenuation at low voltage.•Assessment of dimethyl sulfoxide concentration and distribution in cryopreserved ovarian tissue by X-ray Computed Tomography. |
| doi_str_mv | 10.1016/j.theriogenology.2018.06.031 |
| format | Article |
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•Ovarian tissue cryopreservation as fertility preservation options for cancer patients.•Monitoring of cryopreservation procedures by X-ray Computed Tomography.•Understanding and optimization of controlled rate slow cooling protocols for tissue cryopreservation.•Dimethyl sulfoxide concentration proportional to X ray attenuation at low voltage.•Assessment of dimethyl sulfoxide concentration and distribution in cryopreserved ovarian tissue by X-ray Computed Tomography.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2018.06.031</identifier><identifier>PMID: 30015147</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cattle ; Cold Temperature ; Cryopreservation - methods ; Cryopreservation - veterinary ; Cryoprotectant visualization ; CT imaging ; Female ; Fertility preservation ; Methanol - pharmacology ; Ovary ; Time Factors ; Tissue permeation ; Tissue Preservation - veterinary ; Tomography, X-Ray Computed - methods ; Tomography, X-Ray Computed - veterinary</subject><ispartof>Theriogenology, 2018-10, Vol.119, p.183-188</ispartof><rights>2018 Elsevier Inc.</rights><rights>Copyright © 2018 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-36641f6046928d3e1529ebad2765a5aa692f6ac3b557d6d573e3ff75dfe988883</citedby><cites>FETCH-LOGICAL-c386t-36641f6046928d3e1529ebad2765a5aa692f6ac3b557d6d573e3ff75dfe988883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2018.06.031$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30015147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Corral, Ariadna</creatorcontrib><creatorcontrib>Balcerzyk, Marcin</creatorcontrib><creatorcontrib>Gallardo, Miguel</creatorcontrib><creatorcontrib>Amorim, Christiani A.</creatorcontrib><creatorcontrib>Parrado-Gallego, Ángel</creatorcontrib><creatorcontrib>Risco, Ramón</creatorcontrib><title>An optimized controlled rate slow cooling protocol for bovine ovarian tissue cryopreservation by means of X-ray computed tomography</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>Cryopreservation and subsequent transplantation of ovarian tissue is the only option to preserve fertility in certain patients facing gonadotoxic treatment. So far, cryopreservation of ovarian tissue has been carried out mostly by a controlled rate slow cooling process, typically known as slow freezing. Even though there are still some concerns about the iatrogenic damage on the follicle population, this technique has been used in the more than 100 live births reported to date. It is well known that the control of the cryoprotectant loading in the tissue is crucial to in a cryopreservation procedure. We have used the technology of X-ray computed tomography to assess the concentration and distribution of dimethyl sulfoxide (one of the cryoprotectants most used in fertility preservation) inside pieces of bovine ovarian tissue after its cryopreservation. The low voltage used in our device (75 kV) and the high electronic density of this cryoprotectant makes the X-ray attenuation proportional to its concentration. By assessing and comparing the permeation and homogeneity of the cryoprotectant inside ovarian tissue fragments subjected to a controlled rate slow cooling process, we have characterized the effect of variations in the main parameters involved in the process, with the goal of achieving an optimized protocol with higher permeation of the cryoprotectant in the tissue. The most promissory results were obtained by increasing the initial concentration of dimethyl sulfoxide in the vehicle solution from 10 to 20%v/v.
•Ovarian tissue cryopreservation as fertility preservation options for cancer patients.•Monitoring of cryopreservation procedures by X-ray Computed Tomography.•Understanding and optimization of controlled rate slow cooling protocols for tissue cryopreservation.•Dimethyl sulfoxide concentration proportional to X ray attenuation at low voltage.•Assessment of dimethyl sulfoxide concentration and distribution in cryopreserved ovarian tissue by X-ray Computed Tomography.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cold Temperature</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotectant visualization</subject><subject>CT imaging</subject><subject>Female</subject><subject>Fertility preservation</subject><subject>Methanol - pharmacology</subject><subject>Ovary</subject><subject>Time Factors</subject><subject>Tissue permeation</subject><subject>Tissue Preservation - veterinary</subject><subject>Tomography, X-Ray Computed - methods</subject><subject>Tomography, X-Ray Computed - veterinary</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUcGO1DAMjRCIHRZ-AeXAgUtL0kzSVuKyWrGAtBIXkPYWpak7m1EalyQdVK78OFnNgsQNX2w9PfvZfoS84azmjKt3xzrfQ3R4gIAeD1vdMN7VTNVM8Cdkx7u2r0Qj-FOyY6wXler53QV5kdKRMSaU4s_JhWCMS75vd-TXVaC4ZDe7nzBSiyFH9L6U0WSgyeOPAqJ34UCXiBktejphpAOeXACKJxOdCTS7lFagNm64REgQTyY7DHTY6AwmJIoTvaui2cq0eVlzEcg44yGa5X57SZ5Nxid49ZgvybebD1-vP1W3Xz5-vr66razoVK7K7ns-KbZXfdONArhsehjM2LRKGmlMgSdlrBikbEc1ylaAmKZWjhP0XQlxSd6e55ZLvq-Qsp5dsuC9CYBr0g1ruVRNJ2Shvj9TbcSUIkx6iW42cdOc6Qcb9FH_a4N-sEEzpYsNpf31o9I6zDD-bf7z90K4OROg3HtyEHWyDoKF0UWwWY_o_k_pN-8Gpcs</recordid><startdate>20181001</startdate><enddate>20181001</enddate><creator>Corral, Ariadna</creator><creator>Balcerzyk, Marcin</creator><creator>Gallardo, Miguel</creator><creator>Amorim, Christiani A.</creator><creator>Parrado-Gallego, Ángel</creator><creator>Risco, Ramón</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20181001</creationdate><title>An optimized controlled rate slow cooling protocol for bovine ovarian tissue cryopreservation by means of X-ray computed tomography</title><author>Corral, Ariadna ; 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By assessing and comparing the permeation and homogeneity of the cryoprotectant inside ovarian tissue fragments subjected to a controlled rate slow cooling process, we have characterized the effect of variations in the main parameters involved in the process, with the goal of achieving an optimized protocol with higher permeation of the cryoprotectant in the tissue. The most promissory results were obtained by increasing the initial concentration of dimethyl sulfoxide in the vehicle solution from 10 to 20%v/v.
•Ovarian tissue cryopreservation as fertility preservation options for cancer patients.•Monitoring of cryopreservation procedures by X-ray Computed Tomography.•Understanding and optimization of controlled rate slow cooling protocols for tissue cryopreservation.•Dimethyl sulfoxide concentration proportional to X ray attenuation at low voltage.•Assessment of dimethyl sulfoxide concentration and distribution in cryopreserved ovarian tissue by X-ray Computed Tomography.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>30015147</pmid><doi>10.1016/j.theriogenology.2018.06.031</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Cattle Cold Temperature Cryopreservation - methods Cryopreservation - veterinary Cryoprotectant visualization CT imaging Female Fertility preservation Methanol - pharmacology Ovary Time Factors Tissue permeation Tissue Preservation - veterinary Tomography, X-Ray Computed - methods Tomography, X-Ray Computed - veterinary |
| title | An optimized controlled rate slow cooling protocol for bovine ovarian tissue cryopreservation by means of X-ray computed tomography |
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