SlyA regulates the collagenase-mediated cytopathic phenotype in multiresistant Salmonella
Salmonella enterica serotype Typhimurium phagetype DT104 (DT104) is a foodborne pathogen with a multiresistant phenotype conferred by a genomic-based integron structure designated as SGI1. Recently, a novel cytopathic phenotype was ascribed to several isolates of DT104 recovered from veal calves. Th...
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| Veröffentlicht in: | Microbial pathogenesis 2005-04, Vol.38 (4), p.181-187 |
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| creator | Carlson, Steve A. McCuddin, Zoe P. Wu, Max T. |
| description | Salmonella enterica serotype Typhimurium phagetype DT104 (DT104) is a foodborne pathogen with a multiresistant phenotype conferred by a genomic-based integron structure designated as SGI1. Recently, a novel cytopathic phenotype was ascribed to several isolates of DT104 recovered from veal calves. This phenotype is dependent upon
clg, a gene encoding a collagenase in
Salmonella. Using a novel transposon system and an RT-PCR assay for detection of
clg expression, we identified SlyA as a regulator of the collagenase-mediated phenotype. The function of SlyA, in regards to
clg expression, is to repress the synthesis of Clg. Derepression ensued in the absence of SlyA or in the presence of a truncated version of SlyA with the latter being relevant for maintenance of another virulence aspect mediated by SlyA, i.e. survival within macrophages. The SlyA-mediated effect on
clg expression was restricted to DT104 and other
Salmonella phagetypes and serotypes possessing SGI1 thus suggesting co-regulation by an SGI1-specific component. |
| doi_str_mv | 10.1016/j.micpath.2005.01.004 |
| format | Article |
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clg, a gene encoding a collagenase in
Salmonella. Using a novel transposon system and an RT-PCR assay for detection of
clg expression, we identified SlyA as a regulator of the collagenase-mediated phenotype. The function of SlyA, in regards to
clg expression, is to repress the synthesis of Clg. Derepression ensued in the absence of SlyA or in the presence of a truncated version of SlyA with the latter being relevant for maintenance of another virulence aspect mediated by SlyA, i.e. survival within macrophages. The SlyA-mediated effect on
clg expression was restricted to DT104 and other
Salmonella phagetypes and serotypes possessing SGI1 thus suggesting co-regulation by an SGI1-specific component.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2005.01.004</identifier><identifier>PMID: 15797813</identifier><identifier>CODEN: MIPAEV</identifier><language>eng</language><publisher>Oxford: Elsevier India Pvt Ltd</publisher><subject>Amino Acid Sequence ; animal pathogenic bacteria ; Animals ; Antibiotic resistance ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Base Sequence ; Biological and medical sciences ; calves ; Cattle ; Cattle Diseases - microbiology ; Cell Line ; collagenase ; Collagenases - genetics ; Collagenases - metabolism ; Cytopathic ; cytopathic phenotypes ; DNA Transposable Elements - genetics ; Drug Resistance, Multiple, Bacterial ; DT104 ; food pathogens ; Fundamental and applied biological sciences. Psychology ; Humans ; Integrons ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; multiple drug resistance ; Mutagenesis, Insertional ; phenotype ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; RNA, Bacterial - chemistry ; RNA, Bacterial - genetics ; Salmonella ; Salmonella enterica ; Salmonella enterica subsp. enterica serovar Typhimurium ; Salmonella Infections, Animal - microbiology ; Salmonella typhimurium - enzymology ; Salmonella typhimurium - genetics ; Salmonella typhimurium - growth & development ; Sequence Alignment ; Transcription Factors - genetics ; Transcription Factors - metabolism ; veal</subject><ispartof>Microbial pathogenesis, 2005-04, Vol.38 (4), p.181-187</ispartof><rights>2005 Elsevier Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-4a80a4f11e03a7538d1f9c0aa632fb9f180caf54b4ea2a93aa5ddeec0922ee203</citedby><cites>FETCH-LOGICAL-c479t-4a80a4f11e03a7538d1f9c0aa632fb9f180caf54b4ea2a93aa5ddeec0922ee203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2005.01.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16678567$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15797813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carlson, Steve A.</creatorcontrib><creatorcontrib>McCuddin, Zoe P.</creatorcontrib><creatorcontrib>Wu, Max T.</creatorcontrib><title>SlyA regulates the collagenase-mediated cytopathic phenotype in multiresistant Salmonella</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Salmonella enterica serotype Typhimurium phagetype DT104 (DT104) is a foodborne pathogen with a multiresistant phenotype conferred by a genomic-based integron structure designated as SGI1. Recently, a novel cytopathic phenotype was ascribed to several isolates of DT104 recovered from veal calves. This phenotype is dependent upon
clg, a gene encoding a collagenase in
Salmonella. Using a novel transposon system and an RT-PCR assay for detection of
clg expression, we identified SlyA as a regulator of the collagenase-mediated phenotype. The function of SlyA, in regards to
clg expression, is to repress the synthesis of Clg. Derepression ensued in the absence of SlyA or in the presence of a truncated version of SlyA with the latter being relevant for maintenance of another virulence aspect mediated by SlyA, i.e. survival within macrophages. The SlyA-mediated effect on
clg expression was restricted to DT104 and other
Salmonella phagetypes and serotypes possessing SGI1 thus suggesting co-regulation by an SGI1-specific component.</description><subject>Amino Acid Sequence</subject><subject>animal pathogenic bacteria</subject><subject>Animals</subject><subject>Antibiotic resistance</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>calves</subject><subject>Cattle</subject><subject>Cattle Diseases - microbiology</subject><subject>Cell Line</subject><subject>collagenase</subject><subject>Collagenases - genetics</subject><subject>Collagenases - metabolism</subject><subject>Cytopathic</subject><subject>cytopathic phenotypes</subject><subject>DNA Transposable Elements - genetics</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>DT104</subject><subject>food pathogens</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Integrons</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>multiple drug resistance</subject><subject>Mutagenesis, Insertional</subject><subject>phenotype</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>RNA, Bacterial - chemistry</subject><subject>RNA, Bacterial - genetics</subject><subject>Salmonella</subject><subject>Salmonella enterica</subject><subject>Salmonella enterica subsp. enterica serovar Typhimurium</subject><subject>Salmonella Infections, Animal - microbiology</subject><subject>Salmonella typhimurium - enzymology</subject><subject>Salmonella typhimurium - genetics</subject><subject>Salmonella typhimurium - growth & development</subject><subject>Sequence Alignment</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>veal</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vFDEMhkcIRLeFnwDMBW4z2Jmv5ISqCgpSJQ5LD5wib8azm9V8kWQq7b8nqx2px5588PPa1uMk-YCQI2D99ZgP1swUDrkAqHLAHKB8lWwQVJ2hAPk62YCUIisB4Sq59v4IAKos1NvkCqtGNRKLTfJ3259uU8f7pafAPg0HTs3U97TnkTxnA7c2NtrUnMJ0XmdNOh94nMJp5tSO6bD0wTr21gcaQ7qlfphGjgPeJW866j2_X-tN8vjj-5-7n9nD7_tfd7cPmSkbFbKSJFDZITIU1FSFbLFTBojqQnQ71aEEQ11V7komQaogqtqW2YASgllAcZN8ucyd3fRvYR_0YL05XzDytHgtoMECpXwRxKZCkKgiWF1A4ybvHXd6dnYgd9II-ixfH_UqX5_la0Ad5cfcx3XBsovenlOr7Qh8XgHyhvrO0Wisf-bqupFV3UTu04XraNK0d5F53ArAIj5QqUaJSHy7EBzNPll22hvLo4nfcmyCbif7wrH_ASDssGY</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Carlson, Steve A.</creator><creator>McCuddin, Zoe P.</creator><creator>Wu, Max T.</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7T2</scope><scope>7U2</scope></search><sort><creationdate>20050401</creationdate><title>SlyA regulates the collagenase-mediated cytopathic phenotype in multiresistant Salmonella</title><author>Carlson, Steve A. ; McCuddin, Zoe P. ; Wu, Max T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-4a80a4f11e03a7538d1f9c0aa632fb9f180caf54b4ea2a93aa5ddeec0922ee203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>animal pathogenic bacteria</topic><topic>Animals</topic><topic>Antibiotic resistance</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>calves</topic><topic>Cattle</topic><topic>Cattle Diseases - microbiology</topic><topic>Cell Line</topic><topic>collagenase</topic><topic>Collagenases - genetics</topic><topic>Collagenases - metabolism</topic><topic>Cytopathic</topic><topic>cytopathic phenotypes</topic><topic>DNA Transposable Elements - genetics</topic><topic>Drug Resistance, Multiple, Bacterial</topic><topic>DT104</topic><topic>food pathogens</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Integrons</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>multiple drug resistance</topic><topic>Mutagenesis, Insertional</topic><topic>phenotype</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>RNA, Bacterial - chemistry</topic><topic>RNA, Bacterial - genetics</topic><topic>Salmonella</topic><topic>Salmonella enterica</topic><topic>Salmonella enterica subsp. enterica serovar Typhimurium</topic><topic>Salmonella Infections, Animal - microbiology</topic><topic>Salmonella typhimurium - enzymology</topic><topic>Salmonella typhimurium - genetics</topic><topic>Salmonella typhimurium - growth & development</topic><topic>Sequence Alignment</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>veal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carlson, Steve A.</creatorcontrib><creatorcontrib>McCuddin, Zoe P.</creatorcontrib><creatorcontrib>Wu, Max T.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carlson, Steve A.</au><au>McCuddin, Zoe P.</au><au>Wu, Max T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SlyA regulates the collagenase-mediated cytopathic phenotype in multiresistant Salmonella</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2005-04-01</date><risdate>2005</risdate><volume>38</volume><issue>4</issue><spage>181</spage><epage>187</epage><pages>181-187</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><coden>MIPAEV</coden><abstract>Salmonella enterica serotype Typhimurium phagetype DT104 (DT104) is a foodborne pathogen with a multiresistant phenotype conferred by a genomic-based integron structure designated as SGI1. Recently, a novel cytopathic phenotype was ascribed to several isolates of DT104 recovered from veal calves. This phenotype is dependent upon
clg, a gene encoding a collagenase in
Salmonella. Using a novel transposon system and an RT-PCR assay for detection of
clg expression, we identified SlyA as a regulator of the collagenase-mediated phenotype. The function of SlyA, in regards to
clg expression, is to repress the synthesis of Clg. Derepression ensued in the absence of SlyA or in the presence of a truncated version of SlyA with the latter being relevant for maintenance of another virulence aspect mediated by SlyA, i.e. survival within macrophages. The SlyA-mediated effect on
clg expression was restricted to DT104 and other
Salmonella phagetypes and serotypes possessing SGI1 thus suggesting co-regulation by an SGI1-specific component.</abstract><cop>Oxford</cop><pub>Elsevier India Pvt Ltd</pub><pmid>15797813</pmid><doi>10.1016/j.micpath.2005.01.004</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence animal pathogenic bacteria Animals Antibiotic resistance Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Base Sequence Biological and medical sciences calves Cattle Cattle Diseases - microbiology Cell Line collagenase Collagenases - genetics Collagenases - metabolism Cytopathic cytopathic phenotypes DNA Transposable Elements - genetics Drug Resistance, Multiple, Bacterial DT104 food pathogens Fundamental and applied biological sciences. Psychology Humans Integrons Microbiology Miscellaneous Molecular Sequence Data multiple drug resistance Mutagenesis, Insertional phenotype Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA, Bacterial - chemistry RNA, Bacterial - genetics Salmonella Salmonella enterica Salmonella enterica subsp. enterica serovar Typhimurium Salmonella Infections, Animal - microbiology Salmonella typhimurium - enzymology Salmonella typhimurium - genetics Salmonella typhimurium - growth & development Sequence Alignment Transcription Factors - genetics Transcription Factors - metabolism veal |
| title | SlyA regulates the collagenase-mediated cytopathic phenotype in multiresistant Salmonella |
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