Antibacterial effects and cytotoxicity of an adhesive containing low concentration of silver nanoparticles
To evaluate the antibacterial effects, cytotoxicity and microtensile bond strength of an adhesive containing low concentrations of silver nanoparticles (NAg). Various concentrations of NAg (50, 100, 150, 200 and 250 ppm) were incorporated into the primer of the Scotchbond Multi-Purpose adhesive syst...
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| Veröffentlicht in: | Journal of dentistry 2018-10, Vol.77, p.66-71 |
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| creator | Dutra-Correa, Maristela Leite, Alessandra A.B.V. de Cara, Sueli P.H.M. Diniz, Ivana M.A. Marques, Marcia M. Suffredini, Ivana B. Fernandes, Marina S. Toma, Sergio H. Araki, Koiti Medeiros, Igor S. |
| description | To evaluate the antibacterial effects, cytotoxicity and microtensile bond strength of an adhesive containing low concentrations of silver nanoparticles (NAg).
Various concentrations of NAg (50, 100, 150, 200 and 250 ppm) were incorporated into the primer of the Scotchbond Multi-Purpose adhesive system (SBMP). Antibacterial activity was examined using a broth microdilution assay to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), agar diffusion assay and the MTT assay was used to examine the biofilm metabolic activity (S. mutans). The Microtensile Bond Test (μTBS) was performed after 24 h, followed by 6-months storage in distilled water. Cytotoxicity was assessed with an MTT reduction assay in human dental pulp stem cells viability after exposure to Nag-conditioned culture media during 0, 24, 48, and 72 h. The results were statistically analyzed (α ≤ 0.05).
MIC was found between NAg 25 and 50 ppm MBC was determined at 50 ppm of NAg. Bacterial activity inhibition was higher than control in all NAg groups compared to control in agar diffusion assay. Biofilm inhibition was statistically higher in 250 ppm NAg than control. All NAg groups and SBMP presented similar cytotoxicity in each period. Adhesives with NAg 200 and 250 ppm and SBMP (control) presented the highest μTBS values, similar to that of SBMP control, in both instances (24 h and 6 months) (p > 0.05).
The commercial primer containing NAg 250 ppm showed both antibacterial effect and reliable bond strength with no cytotoxicity increase. The addition of NAg to primers seems promising for the improvement of conventional dental adhesives efficacy.
Clinical Significance: The addition of low concentrations of NAg (250 ppm) to primers were effective to improve antibacterial effect preserving the bond strength and the biocompatibility of the commercial product. NAg/primer association could protect the tooth-adhesive interface increasing dental restoration longevity. |
| doi_str_mv | 10.1016/j.jdent.2018.07.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2070801213</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0300571218302069</els_id><sourcerecordid>2070801213</sourcerecordid><originalsourceid>FETCH-LOGICAL-c453t-2a0ba9e807c6dd5bd6c87a9ca16360031b1ee0e108a3661264de932d0187d58e3</originalsourceid><addsrcrecordid>eNp9kbFuFDEQhi0EIpfAEyChlWjS7DK2b21vQRFFSUCKRAMSneW1Z8GrPfuwfRfu7fHlAgUFlTWjb8aj7yfkDYWOAhXv5252GErHgKoOZAcUnpEVVXJoqRTfnpMVcIC2l5SdkfOcZwBYAxtekrPah0H1ckXmq1D8aGzB5M3S4DShLbkxwTX2UGKJv7z15dDEqfYa435g9ntsbAzF-ODD92aJD8fS1lOSKT6GI5v9ssfUBBPi1qTi7YL5FXkxmSXj66f3gny9vfly_bG9_3z36frqvrXrnpeWGRjNgAqkFc71oxNWSTNYQwUXAJyOFBGQgjJcCMrE2uHAmasWpOsV8gtyedq7TfHnDnPRG58tLosJGHdZM5CggDLKK_ruH3SOuxTqdZpRYIqtq8lK8RNlU8w54aS3yW9MOmgK-hiFnvVjFPoYhQapaxR16u3T7t24Qfd35o_7Cnw4AVhl7D0mna3HKtL5VEPQLvr_fvAbfnScPA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2102824176</pqid></control><display><type>article</type><title>Antibacterial effects and cytotoxicity of an adhesive containing low concentration of silver nanoparticles</title><source>Access via ScienceDirect (Elsevier)</source><creator>Dutra-Correa, Maristela ; Leite, Alessandra A.B.V. ; de Cara, Sueli P.H.M. ; Diniz, Ivana M.A. ; Marques, Marcia M. ; Suffredini, Ivana B. ; Fernandes, Marina S. ; Toma, Sergio H. ; Araki, Koiti ; Medeiros, Igor S.</creator><creatorcontrib>Dutra-Correa, Maristela ; Leite, Alessandra A.B.V. ; de Cara, Sueli P.H.M. ; Diniz, Ivana M.A. ; Marques, Marcia M. ; Suffredini, Ivana B. ; Fernandes, Marina S. ; Toma, Sergio H. ; Araki, Koiti ; Medeiros, Igor S.</creatorcontrib><description>To evaluate the antibacterial effects, cytotoxicity and microtensile bond strength of an adhesive containing low concentrations of silver nanoparticles (NAg).
Various concentrations of NAg (50, 100, 150, 200 and 250 ppm) were incorporated into the primer of the Scotchbond Multi-Purpose adhesive system (SBMP). Antibacterial activity was examined using a broth microdilution assay to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), agar diffusion assay and the MTT assay was used to examine the biofilm metabolic activity (S. mutans). The Microtensile Bond Test (μTBS) was performed after 24 h, followed by 6-months storage in distilled water. Cytotoxicity was assessed with an MTT reduction assay in human dental pulp stem cells viability after exposure to Nag-conditioned culture media during 0, 24, 48, and 72 h. The results were statistically analyzed (α ≤ 0.05).
MIC was found between NAg 25 and 50 ppm MBC was determined at 50 ppm of NAg. Bacterial activity inhibition was higher than control in all NAg groups compared to control in agar diffusion assay. Biofilm inhibition was statistically higher in 250 ppm NAg than control. All NAg groups and SBMP presented similar cytotoxicity in each period. Adhesives with NAg 200 and 250 ppm and SBMP (control) presented the highest μTBS values, similar to that of SBMP control, in both instances (24 h and 6 months) (p > 0.05).
The commercial primer containing NAg 250 ppm showed both antibacterial effect and reliable bond strength with no cytotoxicity increase. The addition of NAg to primers seems promising for the improvement of conventional dental adhesives efficacy.
Clinical Significance: The addition of low concentrations of NAg (250 ppm) to primers were effective to improve antibacterial effect preserving the bond strength and the biocompatibility of the commercial product. NAg/primer association could protect the tooth-adhesive interface increasing dental restoration longevity.</description><identifier>ISSN: 0300-5712</identifier><identifier>EISSN: 1879-176X</identifier><identifier>DOI: 10.1016/j.jdent.2018.07.010</identifier><identifier>PMID: 30009857</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adhesion tests ; Adhesive strength ; Adhesives ; Antibacterial activity ; Antibacterial effect ; Antimicrobial agents ; Assaying ; Bacteria ; Biocompatibility ; Biofilms ; Bond strength ; Bonding strength ; Cell culture ; Composite materials ; Conditioning ; Culture media ; Cytotoxicity ; Dental adhesive ; Dental materials ; Dental pulp ; Dental restorative materials ; Dentin ; Dentistry ; Distilled water ; Low concentrations ; Microtensile bond strength ; Minimum inhibitory concentration ; Nanoparticles ; Polyvinyl alcohol ; Primers ; Resins ; Silver ; Silver nanoparticles ; Stem cells ; Studies ; Teeth ; Toxicity ; Viability</subject><ispartof>Journal of dentistry, 2018-10, Vol.77, p.66-71</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Oct 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-2a0ba9e807c6dd5bd6c87a9ca16360031b1ee0e108a3661264de932d0187d58e3</citedby><cites>FETCH-LOGICAL-c453t-2a0ba9e807c6dd5bd6c87a9ca16360031b1ee0e108a3661264de932d0187d58e3</cites><orcidid>0000-0002-9398-1252 ; 0000-0002-7862-4767 ; 0000-0003-3485-4592 ; 0000-0002-5237-101X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jdent.2018.07.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30009857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dutra-Correa, Maristela</creatorcontrib><creatorcontrib>Leite, Alessandra A.B.V.</creatorcontrib><creatorcontrib>de Cara, Sueli P.H.M.</creatorcontrib><creatorcontrib>Diniz, Ivana M.A.</creatorcontrib><creatorcontrib>Marques, Marcia M.</creatorcontrib><creatorcontrib>Suffredini, Ivana B.</creatorcontrib><creatorcontrib>Fernandes, Marina S.</creatorcontrib><creatorcontrib>Toma, Sergio H.</creatorcontrib><creatorcontrib>Araki, Koiti</creatorcontrib><creatorcontrib>Medeiros, Igor S.</creatorcontrib><title>Antibacterial effects and cytotoxicity of an adhesive containing low concentration of silver nanoparticles</title><title>Journal of dentistry</title><addtitle>J Dent</addtitle><description>To evaluate the antibacterial effects, cytotoxicity and microtensile bond strength of an adhesive containing low concentrations of silver nanoparticles (NAg).
Various concentrations of NAg (50, 100, 150, 200 and 250 ppm) were incorporated into the primer of the Scotchbond Multi-Purpose adhesive system (SBMP). Antibacterial activity was examined using a broth microdilution assay to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), agar diffusion assay and the MTT assay was used to examine the biofilm metabolic activity (S. mutans). The Microtensile Bond Test (μTBS) was performed after 24 h, followed by 6-months storage in distilled water. Cytotoxicity was assessed with an MTT reduction assay in human dental pulp stem cells viability after exposure to Nag-conditioned culture media during 0, 24, 48, and 72 h. The results were statistically analyzed (α ≤ 0.05).
MIC was found between NAg 25 and 50 ppm MBC was determined at 50 ppm of NAg. Bacterial activity inhibition was higher than control in all NAg groups compared to control in agar diffusion assay. Biofilm inhibition was statistically higher in 250 ppm NAg than control. All NAg groups and SBMP presented similar cytotoxicity in each period. Adhesives with NAg 200 and 250 ppm and SBMP (control) presented the highest μTBS values, similar to that of SBMP control, in both instances (24 h and 6 months) (p > 0.05).
The commercial primer containing NAg 250 ppm showed both antibacterial effect and reliable bond strength with no cytotoxicity increase. The addition of NAg to primers seems promising for the improvement of conventional dental adhesives efficacy.
Clinical Significance: The addition of low concentrations of NAg (250 ppm) to primers were effective to improve antibacterial effect preserving the bond strength and the biocompatibility of the commercial product. NAg/primer association could protect the tooth-adhesive interface increasing dental restoration longevity.</description><subject>Adhesion tests</subject><subject>Adhesive strength</subject><subject>Adhesives</subject><subject>Antibacterial activity</subject><subject>Antibacterial effect</subject><subject>Antimicrobial agents</subject><subject>Assaying</subject><subject>Bacteria</subject><subject>Biocompatibility</subject><subject>Biofilms</subject><subject>Bond strength</subject><subject>Bonding strength</subject><subject>Cell culture</subject><subject>Composite materials</subject><subject>Conditioning</subject><subject>Culture media</subject><subject>Cytotoxicity</subject><subject>Dental adhesive</subject><subject>Dental materials</subject><subject>Dental pulp</subject><subject>Dental restorative materials</subject><subject>Dentin</subject><subject>Dentistry</subject><subject>Distilled water</subject><subject>Low concentrations</subject><subject>Microtensile bond strength</subject><subject>Minimum inhibitory concentration</subject><subject>Nanoparticles</subject><subject>Polyvinyl alcohol</subject><subject>Primers</subject><subject>Resins</subject><subject>Silver</subject><subject>Silver nanoparticles</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Teeth</subject><subject>Toxicity</subject><subject>Viability</subject><issn>0300-5712</issn><issn>1879-176X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kbFuFDEQhi0EIpfAEyChlWjS7DK2b21vQRFFSUCKRAMSneW1Z8GrPfuwfRfu7fHlAgUFlTWjb8aj7yfkDYWOAhXv5252GErHgKoOZAcUnpEVVXJoqRTfnpMVcIC2l5SdkfOcZwBYAxtekrPah0H1ckXmq1D8aGzB5M3S4DShLbkxwTX2UGKJv7z15dDEqfYa435g9ntsbAzF-ODD92aJD8fS1lOSKT6GI5v9ssfUBBPi1qTi7YL5FXkxmSXj66f3gny9vfly_bG9_3z36frqvrXrnpeWGRjNgAqkFc71oxNWSTNYQwUXAJyOFBGQgjJcCMrE2uHAmasWpOsV8gtyedq7TfHnDnPRG58tLosJGHdZM5CggDLKK_ruH3SOuxTqdZpRYIqtq8lK8RNlU8w54aS3yW9MOmgK-hiFnvVjFPoYhQapaxR16u3T7t24Qfd35o_7Cnw4AVhl7D0mna3HKtL5VEPQLvr_fvAbfnScPA</recordid><startdate>201810</startdate><enddate>201810</enddate><creator>Dutra-Correa, Maristela</creator><creator>Leite, Alessandra A.B.V.</creator><creator>de Cara, Sueli P.H.M.</creator><creator>Diniz, Ivana M.A.</creator><creator>Marques, Marcia M.</creator><creator>Suffredini, Ivana B.</creator><creator>Fernandes, Marina S.</creator><creator>Toma, Sergio H.</creator><creator>Araki, Koiti</creator><creator>Medeiros, Igor S.</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9398-1252</orcidid><orcidid>https://orcid.org/0000-0002-7862-4767</orcidid><orcidid>https://orcid.org/0000-0003-3485-4592</orcidid><orcidid>https://orcid.org/0000-0002-5237-101X</orcidid></search><sort><creationdate>201810</creationdate><title>Antibacterial effects and cytotoxicity of an adhesive containing low concentration of silver nanoparticles</title><author>Dutra-Correa, Maristela ; Leite, Alessandra A.B.V. ; de Cara, Sueli P.H.M. ; Diniz, Ivana M.A. ; Marques, Marcia M. ; Suffredini, Ivana B. ; Fernandes, Marina S. ; Toma, Sergio H. ; Araki, Koiti ; Medeiros, Igor S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-2a0ba9e807c6dd5bd6c87a9ca16360031b1ee0e108a3661264de932d0187d58e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adhesion tests</topic><topic>Adhesive strength</topic><topic>Adhesives</topic><topic>Antibacterial activity</topic><topic>Antibacterial effect</topic><topic>Antimicrobial agents</topic><topic>Assaying</topic><topic>Bacteria</topic><topic>Biocompatibility</topic><topic>Biofilms</topic><topic>Bond strength</topic><topic>Bonding strength</topic><topic>Cell culture</topic><topic>Composite materials</topic><topic>Conditioning</topic><topic>Culture media</topic><topic>Cytotoxicity</topic><topic>Dental adhesive</topic><topic>Dental materials</topic><topic>Dental pulp</topic><topic>Dental restorative materials</topic><topic>Dentin</topic><topic>Dentistry</topic><topic>Distilled water</topic><topic>Low concentrations</topic><topic>Microtensile bond strength</topic><topic>Minimum inhibitory concentration</topic><topic>Nanoparticles</topic><topic>Polyvinyl alcohol</topic><topic>Primers</topic><topic>Resins</topic><topic>Silver</topic><topic>Silver nanoparticles</topic><topic>Stem cells</topic><topic>Studies</topic><topic>Teeth</topic><topic>Toxicity</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dutra-Correa, Maristela</creatorcontrib><creatorcontrib>Leite, Alessandra A.B.V.</creatorcontrib><creatorcontrib>de Cara, Sueli P.H.M.</creatorcontrib><creatorcontrib>Diniz, Ivana M.A.</creatorcontrib><creatorcontrib>Marques, Marcia M.</creatorcontrib><creatorcontrib>Suffredini, Ivana B.</creatorcontrib><creatorcontrib>Fernandes, Marina S.</creatorcontrib><creatorcontrib>Toma, Sergio H.</creatorcontrib><creatorcontrib>Araki, Koiti</creatorcontrib><creatorcontrib>Medeiros, Igor S.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dentistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dutra-Correa, Maristela</au><au>Leite, Alessandra A.B.V.</au><au>de Cara, Sueli P.H.M.</au><au>Diniz, Ivana M.A.</au><au>Marques, Marcia M.</au><au>Suffredini, Ivana B.</au><au>Fernandes, Marina S.</au><au>Toma, Sergio H.</au><au>Araki, Koiti</au><au>Medeiros, Igor S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial effects and cytotoxicity of an adhesive containing low concentration of silver nanoparticles</atitle><jtitle>Journal of dentistry</jtitle><addtitle>J Dent</addtitle><date>2018-10</date><risdate>2018</risdate><volume>77</volume><spage>66</spage><epage>71</epage><pages>66-71</pages><issn>0300-5712</issn><eissn>1879-176X</eissn><abstract>To evaluate the antibacterial effects, cytotoxicity and microtensile bond strength of an adhesive containing low concentrations of silver nanoparticles (NAg).
Various concentrations of NAg (50, 100, 150, 200 and 250 ppm) were incorporated into the primer of the Scotchbond Multi-Purpose adhesive system (SBMP). Antibacterial activity was examined using a broth microdilution assay to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), agar diffusion assay and the MTT assay was used to examine the biofilm metabolic activity (S. mutans). The Microtensile Bond Test (μTBS) was performed after 24 h, followed by 6-months storage in distilled water. Cytotoxicity was assessed with an MTT reduction assay in human dental pulp stem cells viability after exposure to Nag-conditioned culture media during 0, 24, 48, and 72 h. The results were statistically analyzed (α ≤ 0.05).
MIC was found between NAg 25 and 50 ppm MBC was determined at 50 ppm of NAg. Bacterial activity inhibition was higher than control in all NAg groups compared to control in agar diffusion assay. Biofilm inhibition was statistically higher in 250 ppm NAg than control. All NAg groups and SBMP presented similar cytotoxicity in each period. Adhesives with NAg 200 and 250 ppm and SBMP (control) presented the highest μTBS values, similar to that of SBMP control, in both instances (24 h and 6 months) (p > 0.05).
The commercial primer containing NAg 250 ppm showed both antibacterial effect and reliable bond strength with no cytotoxicity increase. The addition of NAg to primers seems promising for the improvement of conventional dental adhesives efficacy.
Clinical Significance: The addition of low concentrations of NAg (250 ppm) to primers were effective to improve antibacterial effect preserving the bond strength and the biocompatibility of the commercial product. NAg/primer association could protect the tooth-adhesive interface increasing dental restoration longevity.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>30009857</pmid><doi>10.1016/j.jdent.2018.07.010</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-9398-1252</orcidid><orcidid>https://orcid.org/0000-0002-7862-4767</orcidid><orcidid>https://orcid.org/0000-0003-3485-4592</orcidid><orcidid>https://orcid.org/0000-0002-5237-101X</orcidid></addata></record> |
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| ispartof | Journal of dentistry, 2018-10, Vol.77, p.66-71 |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | Adhesion tests Adhesive strength Adhesives Antibacterial activity Antibacterial effect Antimicrobial agents Assaying Bacteria Biocompatibility Biofilms Bond strength Bonding strength Cell culture Composite materials Conditioning Culture media Cytotoxicity Dental adhesive Dental materials Dental pulp Dental restorative materials Dentin Dentistry Distilled water Low concentrations Microtensile bond strength Minimum inhibitory concentration Nanoparticles Polyvinyl alcohol Primers Resins Silver Silver nanoparticles Stem cells Studies Teeth Toxicity Viability |
| title | Antibacterial effects and cytotoxicity of an adhesive containing low concentration of silver nanoparticles |
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