Susceptibility of some clinical isolates of Staphylococcus aureus to fractions from the aerial parts of Leuzea carthamoides
The antimicrobial activity of the dichloromethane extract from aerial parts of Leuzea carthamoides DC. was tested in vitro against 19 Staphylococcus aureus strains (ATCC 25923, CNCTC Mau 43/60, clinical isolates). The extract was fractionated by column chromatography on silica gel into six fractions...
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| Veröffentlicht in: | Biológia 2008-10, Vol.63 (5), p.607-609 |
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| creator | Janovska, Dagmar Kloucek, Pavel Urban, Jan Vanek, Tomas Rada, Vojtech Kokoska, Ladislav |
| description | The antimicrobial activity of the dichloromethane extract from aerial parts of Leuzea carthamoides DC. was tested in vitro against 19 Staphylococcus aureus strains (ATCC 25923, CNCTC Mau 43/60, clinical isolates). The extract was fractionated by column chromatography on silica gel into six fractions (petroleum ether, toluene, dichloromethane, ethyl acetate, methanol and water). The minimum inhibitory concentrations (MICs) of the fractions ranged from 64 to 1024 µg/mL. An ethyl acetate fraction (EA 1) with the widest range of activity inhibited all of the strains with MIC in the range 128â512 µg/mL. This fraction exhibited potent activity against strains which showed associated resistance to oxacillin, ciprofloxacin and erythromycin. |
| doi_str_mv | 10.2478/s11756-008-0105-0 |
| format | Article |
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The extract was fractionated by column chromatography on silica gel into six fractions (petroleum ether, toluene, dichloromethane, ethyl acetate, methanol and water). The minimum inhibitory concentrations (MICs) of the fractions ranged from 64 to 1024 µg/mL. An ethyl acetate fraction (EA 1) with the widest range of activity inhibited all of the strains with MIC in the range 128â512 µg/mL. 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The extract was fractionated by column chromatography on silica gel into six fractions (petroleum ether, toluene, dichloromethane, ethyl acetate, methanol and water). The minimum inhibitory concentrations (MICs) of the fractions ranged from 64 to 1024 µg/mL. An ethyl acetate fraction (EA 1) with the widest range of activity inhibited all of the strains with MIC in the range 128â512 µg/mL. 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Kloucek, Pavel ; Urban, Jan ; Vanek, Tomas ; Rada, Vojtech ; Kokoska, Ladislav</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-fa4ffadb3c76962a5e9da83ca196815d9f256a5e0c7301d98762dcb2bf6c65d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>aerial parts</topic><topic>anti-infective properties</topic><topic>antimicrobial activity</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>chromatography</topic><topic>ciprofloxacin</topic><topic>erythromycin</topic><topic>ethyl acetate</topic><topic>fractionation</topic><topic>Leuzea carthamoides</topic><topic>Life Sciences</topic><topic>methanol</topic><topic>methylene chloride</topic><topic>Microbiology</topic><topic>minimum inhibitory concentration</topic><topic>MRSA</topic><topic>oxacillin</topic><topic>petroleum</topic><topic>Plant Sciences</topic><topic>Rhaponticum carthamoides</topic><topic>silica gel</topic><topic>Staphylococcus aureus</topic><topic>toluene</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Janovska, Dagmar</creatorcontrib><creatorcontrib>Kloucek, Pavel</creatorcontrib><creatorcontrib>Urban, Jan</creatorcontrib><creatorcontrib>Vanek, Tomas</creatorcontrib><creatorcontrib>Rada, Vojtech</creatorcontrib><creatorcontrib>Kokoska, Ladislav</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biológia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Janovska, Dagmar</au><au>Kloucek, Pavel</au><au>Urban, Jan</au><au>Vanek, Tomas</au><au>Rada, Vojtech</au><au>Kokoska, Ladislav</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Susceptibility of some clinical isolates of Staphylococcus aureus to fractions from the aerial parts of Leuzea carthamoides</atitle><jtitle>Biológia</jtitle><stitle>Biologia</stitle><date>2008-10-01</date><risdate>2008</risdate><volume>63</volume><issue>5</issue><spage>607</spage><epage>609</epage><pages>607-609</pages><issn>1336-9563</issn><issn>0006-3088</issn><eissn>1336-9563</eissn><abstract>The antimicrobial activity of the dichloromethane extract from aerial parts of Leuzea carthamoides DC. was tested in vitro against 19 Staphylococcus aureus strains (ATCC 25923, CNCTC Mau 43/60, clinical isolates). 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| subjects | aerial parts anti-infective properties antimicrobial activity Biomedical and Life Sciences Cell Biology chromatography ciprofloxacin erythromycin ethyl acetate fractionation Leuzea carthamoides Life Sciences methanol methylene chloride Microbiology minimum inhibitory concentration MRSA oxacillin petroleum Plant Sciences Rhaponticum carthamoides silica gel Staphylococcus aureus toluene Zoology |
| title | Susceptibility of some clinical isolates of Staphylococcus aureus to fractions from the aerial parts of Leuzea carthamoides |
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