Comparison of next‐generation droplet digital PCR with quantitative PCR for enumeration of Naegleria fowleri in environmental water and clinical samples

Naegleria fowleri in recreational waters is a serious health threat. A rapid and accurate method to determine this pathogen in water is vital to develop effective control strategies. In this study, we compared two molecular methods: droplet digital polymerase chain reaction (ddPCR) and quantitative...

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Veröffentlicht in:	Letters in applied microbiology 2018-10, Vol.67 (4), p.322-328
Hauptverfasser:	Xue, J., Caton, K., Sherchan, S.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Brackish water Comparative analysis ddPCR Deoxyribonucleic acid DNA Enumeration Genetic testing Health risks Identification methods Lakes LOD Naegleria fowleri Pathogens Polymerase chain reaction primary amoebic meningoencephalitis Protozoa qPCR Recreational waters Risk analysis Risk factors Sampling methods specificity Surface water Technology Water analysis Water sampling
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