Cytogenetic Analysis of the Results of Genome Editing on the Cell Model of Parkinson’s Disease
We performed a cytogenetic analysis of the results of CRISPR/Cas9-correction of G2019S mutation in LRRK2 gene associated with Parkinson’s disease. Genome editing was performed on induced pluripotent stem cells derived from fibroblasts of a patient carrying this mutation. A mosaic variant of tetraplo...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2018-07, Vol.165 (3), p.378-381 |
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| creator | Vetchinova, A. S. Simonova, V. V. Novosadova, E. V. Manuilova, E. S. Nenasheva, V. V. Tarantul, V. Z. Grivennikov, I. A. Khaspekov, L. G. Illarioshkin, S. N. |
| description | We performed a cytogenetic analysis of the results of CRISPR/Cas9-correction of G2019S mutation in
LRRK2
gene associated with Parkinson’s disease. Genome editing was performed on induced pluripotent stem cells derived from fibroblasts of a patient carrying this mutation. A mosaic variant of tetraploidy 92 XXYY/46,XY (24-43% cells from various clones) was found in neuronal precursors differentiated from the induced pluripotent stem cells after gene editing procedure. Solitary cases of translocations and chromosome breaks were observed. These data confirm the importance of the development of new approaches ensuring genome stability in CRISPR/Cas9-edited cultures. |
| doi_str_mv | 10.1007/s10517-018-4174-y |
| format | Article |
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LRRK2
gene associated with Parkinson’s disease. Genome editing was performed on induced pluripotent stem cells derived from fibroblasts of a patient carrying this mutation. A mosaic variant of tetraploidy 92 XXYY/46,XY (24-43% cells from various clones) was found in neuronal precursors differentiated from the induced pluripotent stem cells after gene editing procedure. Solitary cases of translocations and chromosome breaks were observed. These data confirm the importance of the development of new approaches ensuring genome stability in CRISPR/Cas9-edited cultures.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-018-4174-y</identifier><identifier>PMID: 30006877</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Analysis ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Chromosome translocations ; CRISPR ; Cytogenetics ; Fibroblasts ; Genes ; Genetic aspects ; Genetics ; Genome editing ; Genomes ; Genomics ; Internal Medicine ; Laboratory Medicine ; LRRK2 protein ; Mutation ; Neural stem cells ; Parkinson's disease ; Pathology ; Pluripotency ; Stem cells ; Tetraploidy</subject><ispartof>Bulletin of experimental biology and medicine, 2018-07, Vol.165 (3), p.378-381</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Bulletin of Experimental Biology and Medicine is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-60e56c7e777b88567aee842096bbe5fce2cbc3cb43582a969b67a0bcc1abdec93</citedby><cites>FETCH-LOGICAL-c470t-60e56c7e777b88567aee842096bbe5fce2cbc3cb43582a969b67a0bcc1abdec93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-018-4174-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-018-4174-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30006877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vetchinova, A. S.</creatorcontrib><creatorcontrib>Simonova, V. V.</creatorcontrib><creatorcontrib>Novosadova, E. V.</creatorcontrib><creatorcontrib>Manuilova, E. S.</creatorcontrib><creatorcontrib>Nenasheva, V. V.</creatorcontrib><creatorcontrib>Tarantul, V. Z.</creatorcontrib><creatorcontrib>Grivennikov, I. A.</creatorcontrib><creatorcontrib>Khaspekov, L. G.</creatorcontrib><creatorcontrib>Illarioshkin, S. N.</creatorcontrib><title>Cytogenetic Analysis of the Results of Genome Editing on the Cell Model of Parkinson’s Disease</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>We performed a cytogenetic analysis of the results of CRISPR/Cas9-correction of G2019S mutation in
LRRK2
gene associated with Parkinson’s disease. Genome editing was performed on induced pluripotent stem cells derived from fibroblasts of a patient carrying this mutation. A mosaic variant of tetraploidy 92 XXYY/46,XY (24-43% cells from various clones) was found in neuronal precursors differentiated from the induced pluripotent stem cells after gene editing procedure. Solitary cases of translocations and chromosome breaks were observed. These data confirm the importance of the development of new approaches ensuring genome stability in CRISPR/Cas9-edited cultures.</description><subject>Analysis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Chromosome translocations</subject><subject>CRISPR</subject><subject>Cytogenetics</subject><subject>Fibroblasts</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetics</subject><subject>Genome editing</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>LRRK2 protein</subject><subject>Mutation</subject><subject>Neural stem cells</subject><subject>Parkinson's disease</subject><subject>Pathology</subject><subject>Pluripotency</subject><subject>Stem cells</subject><subject>Tetraploidy</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kcFu1DAURS0EokPhA9igSEgVmxTbSWxnORpKQSoCVbA2jvMy45LYJc9ZZNff4Pf4EpxOoRRReWE_-9wrX11CnjN6zCiVr5HRismcMpWXTJb5_ICsWCWLXHHOHpIVTVBeKqUOyBPEi2Wkgj0mB0U6CiXlinzdzDFswUN0Nlt708_oMAtdFneQnQNOfbweT8GHAbKT1kXnt1nw18AG-j77EFroF-aTGb85j8H_vPqB2RuHYBCekked6RGe3eyH5Mvbk8-bd_nZx9P3m_VZbktJYy4oVMJKkFI2SlVCGgBVclqLpoGqs8BtYwvblEWluKlF3SSENtYy07Rg6-KQvNr7Xo7h-wQY9eDQpv8ZD2FCzVN2XkpeFgl9-Q96EaYxZV8oUXPG67q6pbamB-18F-Jo7GKq11VZS6akWLyO_0Ol1cLgbPDQuXR_R3D0l2AHpo87DP0UXfB4F2R70I4BcYROX45uMOOsGdVL_Xpfv07166V-PSfNi5tkUzNA-0fxu-8E8D2A6clvYbyNfr_rL9XPuU8</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Vetchinova, A. 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V. ; Novosadova, E. V. ; Manuilova, E. S. ; Nenasheva, V. V. ; Tarantul, V. Z. ; Grivennikov, I. A. ; Khaspekov, L. G. ; Illarioshkin, S. 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S.</creatorcontrib><creatorcontrib>Simonova, V. V.</creatorcontrib><creatorcontrib>Novosadova, E. V.</creatorcontrib><creatorcontrib>Manuilova, E. S.</creatorcontrib><creatorcontrib>Nenasheva, V. V.</creatorcontrib><creatorcontrib>Tarantul, V. Z.</creatorcontrib><creatorcontrib>Grivennikov, I. A.</creatorcontrib><creatorcontrib>Khaspekov, L. G.</creatorcontrib><creatorcontrib>Illarioshkin, S. 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LRRK2
gene associated with Parkinson’s disease. Genome editing was performed on induced pluripotent stem cells derived from fibroblasts of a patient carrying this mutation. A mosaic variant of tetraploidy 92 XXYY/46,XY (24-43% cells from various clones) was found in neuronal precursors differentiated from the induced pluripotent stem cells after gene editing procedure. Solitary cases of translocations and chromosome breaks were observed. These data confirm the importance of the development of new approaches ensuring genome stability in CRISPR/Cas9-edited cultures.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>30006877</pmid><doi>10.1007/s10517-018-4174-y</doi><tpages>4</tpages></addata></record> |
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| ispartof | Bulletin of experimental biology and medicine, 2018-07, Vol.165 (3), p.378-381 |
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| subjects | Analysis Biomedical and Life Sciences Biomedicine Cell Biology Chromosome translocations CRISPR Cytogenetics Fibroblasts Genes Genetic aspects Genetics Genome editing Genomes Genomics Internal Medicine Laboratory Medicine LRRK2 protein Mutation Neural stem cells Parkinson's disease Pathology Pluripotency Stem cells Tetraploidy |
| title | Cytogenetic Analysis of the Results of Genome Editing on the Cell Model of Parkinson’s Disease |
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