SURVEY OF GRAPEVINE VIRUSES IN CHILE
Grapevines from six Chilean regions were surveyed for virus diseases and tested for the presence of the most important viruses. ELISA testing of 2535 samples and confirmatory RT-PCR of some ELISA-negative samples from symptomatic and symptomless vines gave the following infection rates: 6.36% for Gr...
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Veröffentlicht in: | Journal of plant pathology 2008-03, Vol.90 (1), p.125-130 |
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description | Grapevines from six Chilean regions were surveyed for virus diseases and tested for the presence of the most important viruses. ELISA testing of 2535 samples and confirmatory RT-PCR of some ELISA-negative samples from symptomatic and symptomless vines gave the following infection rates: 6.36% for Grapevine fanleaf virus (GFLV); 4.67% for Grapevine leafroll associated virus 1 (GLRaV-1); 16.05% for Grapevine leafroll associated virus 2 (GLRaV-2); 6.41% for Grapevine leafroll associated virus 3 (GLRaV-3); 0.26% for Grapevine leafroll associated virus 7 (GLRaV-7); 14.99% for Grapevine fleck virus (GFkV); 5.57% for Grapevine virus A (GVA) and 0.78% for Grapevine virus B (GVB). Strawberry latent ringspot virus (SLRSV) Tomato ringspot virus (ToRSV) and Arabis mosaic virus (ArMV) were not detected. Overall infection in the surveyed Chilean grapevines, considering ELISA and RT-PCR, was 32.35%. Virus infection ratio obtained from ELISA analysis in the six regions, varied between 21.19% (Región Metropolitana) and 74.26% (Coquimbo). RT-PCR was used for detection of the Red Globe strain of GLRaV-2 and Grapevine rupestris stem pitting associated virus (GRSPaV), and to confirm and extend ELISA results. GVB, GFkV, GRSPaV, GLRaV-2 RG and GLRaV-7 are new records for Chile. |
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ELISA testing of 2535 samples and confirmatory RT-PCR of some ELISA-negative samples from symptomatic and symptomless vines gave the following infection rates: 6.36% for Grapevine fanleaf virus (GFLV); 4.67% for Grapevine leafroll associated virus 1 (GLRaV-1); 16.05% for Grapevine leafroll associated virus 2 (GLRaV-2); 6.41% for Grapevine leafroll associated virus 3 (GLRaV-3); 0.26% for Grapevine leafroll associated virus 7 (GLRaV-7); 14.99% for Grapevine fleck virus (GFkV); 5.57% for Grapevine virus A (GVA) and 0.78% for Grapevine virus B (GVB). Strawberry latent ringspot virus (SLRSV) Tomato ringspot virus (ToRSV) and Arabis mosaic virus (ArMV) were not detected. Overall infection in the surveyed Chilean grapevines, considering ELISA and RT-PCR, was 32.35%. Virus infection ratio obtained from ELISA analysis in the six regions, varied between 21.19% (Región Metropolitana) and 74.26% (Coquimbo). RT-PCR was used for detection of the Red Globe strain of GLRaV-2 and Grapevine rupestris stem pitting associated virus (GRSPaV), and to confirm and extend ELISA results. GVB, GFkV, GRSPaV, GLRaV-2 RG and GLRaV-7 are new records for Chile.</description><identifier>ISSN: 1125-4653</identifier><identifier>EISSN: 2239-7264</identifier><language>eng</language><publisher>An International Journal of the Italian Phytopathological Society</publisher><subject>Arabis mosaic virus ; Grapevine fanleaf virus ; Grapevine fleck virus ; Grapevine virus A ; Grapevine virus B ; Infections ; Mosaic viruses ; Phytopathology ; Plant diseases ; Plants ; Reverse transcriptase polymerase chain reaction ; Rootstocks ; Satellite viruses ; Short Communication ; Strawberry latent ringspot virus ; Tomato ringspot virus ; Vineyards ; Viruses ; Vitaceae</subject><ispartof>Journal of plant pathology, 2008-03, Vol.90 (1), p.125-130</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/41998470$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41998470$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,57995,58228</link.rule.ids></links><search><creatorcontrib>Fiore, N.</creatorcontrib><creatorcontrib>Prodan, S.</creatorcontrib><creatorcontrib>Montealegre, J.</creatorcontrib><creatorcontrib>Aballay, E.</creatorcontrib><creatorcontrib>Pino, A.M.</creatorcontrib><creatorcontrib>Zamorano, A.</creatorcontrib><title>SURVEY OF GRAPEVINE VIRUSES IN CHILE</title><title>Journal of plant pathology</title><description>Grapevines from six Chilean regions were surveyed for virus diseases and tested for the presence of the most important viruses. ELISA testing of 2535 samples and confirmatory RT-PCR of some ELISA-negative samples from symptomatic and symptomless vines gave the following infection rates: 6.36% for Grapevine fanleaf virus (GFLV); 4.67% for Grapevine leafroll associated virus 1 (GLRaV-1); 16.05% for Grapevine leafroll associated virus 2 (GLRaV-2); 6.41% for Grapevine leafroll associated virus 3 (GLRaV-3); 0.26% for Grapevine leafroll associated virus 7 (GLRaV-7); 14.99% for Grapevine fleck virus (GFkV); 5.57% for Grapevine virus A (GVA) and 0.78% for Grapevine virus B (GVB). Strawberry latent ringspot virus (SLRSV) Tomato ringspot virus (ToRSV) and Arabis mosaic virus (ArMV) were not detected. Overall infection in the surveyed Chilean grapevines, considering ELISA and RT-PCR, was 32.35%. Virus infection ratio obtained from ELISA analysis in the six regions, varied between 21.19% (Región Metropolitana) and 74.26% (Coquimbo). RT-PCR was used for detection of the Red Globe strain of GLRaV-2 and Grapevine rupestris stem pitting associated virus (GRSPaV), and to confirm and extend ELISA results. GVB, GFkV, GRSPaV, GLRaV-2 RG and GLRaV-7 are new records for Chile.</description><subject>Arabis mosaic virus</subject><subject>Grapevine fanleaf virus</subject><subject>Grapevine fleck virus</subject><subject>Grapevine virus A</subject><subject>Grapevine virus B</subject><subject>Infections</subject><subject>Mosaic viruses</subject><subject>Phytopathology</subject><subject>Plant diseases</subject><subject>Plants</subject><subject>Reverse transcriptase polymerase chain reaction</subject><subject>Rootstocks</subject><subject>Satellite viruses</subject><subject>Short Communication</subject><subject>Strawberry latent ringspot virus</subject><subject>Tomato ringspot virus</subject><subject>Vineyards</subject><subject>Viruses</subject><subject>Vitaceae</subject><issn>1125-4653</issn><issn>2239-7264</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNotzEFrgzAYgOEwNphr9xMGHsZuQvIlMX7HImkrSDu0CjtJGiNU7OyMPezfr9Cd3svD-0ACAI6Rglg8koAxkJGIJX8mL973lAqpaByQ97Iqav0V7tfhplh96jrb6bDOiqrUZZjtwnSb5XpJnjozePf63wWp1vqQbqN8v8nSVR71QJM5gtZYYSnKjltnj44eOW2ltKpF3hqHDONOIEcmLFAAcIyDZEwKnnSdNIovyMf9e5nGn6vzc3M-eeuGwXy78eoboDEqRLjBtzvs_TxOzWU6nc302wiGmAhF-R-AZUL7</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Fiore, N.</creator><creator>Prodan, S.</creator><creator>Montealegre, J.</creator><creator>Aballay, E.</creator><creator>Pino, A.M.</creator><creator>Zamorano, A.</creator><general>An International Journal of the Italian Phytopathological Society</general><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20080301</creationdate><title>SURVEY OF GRAPEVINE VIRUSES IN CHILE</title><author>Fiore, N. ; Prodan, S. ; Montealegre, J. ; Aballay, E. ; Pino, A.M. ; Zamorano, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j208t-2dac4c095f3cecbe0b30d55c7d93dae9196f493914c20222e1325115438ff5a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Arabis mosaic virus</topic><topic>Grapevine fanleaf virus</topic><topic>Grapevine fleck virus</topic><topic>Grapevine virus A</topic><topic>Grapevine virus B</topic><topic>Infections</topic><topic>Mosaic viruses</topic><topic>Phytopathology</topic><topic>Plant diseases</topic><topic>Plants</topic><topic>Reverse transcriptase polymerase chain reaction</topic><topic>Rootstocks</topic><topic>Satellite viruses</topic><topic>Short Communication</topic><topic>Strawberry latent ringspot virus</topic><topic>Tomato ringspot virus</topic><topic>Vineyards</topic><topic>Viruses</topic><topic>Vitaceae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fiore, N.</creatorcontrib><creatorcontrib>Prodan, S.</creatorcontrib><creatorcontrib>Montealegre, J.</creatorcontrib><creatorcontrib>Aballay, E.</creatorcontrib><creatorcontrib>Pino, A.M.</creatorcontrib><creatorcontrib>Zamorano, A.</creatorcontrib><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fiore, N.</au><au>Prodan, S.</au><au>Montealegre, J.</au><au>Aballay, E.</au><au>Pino, A.M.</au><au>Zamorano, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SURVEY OF GRAPEVINE VIRUSES IN CHILE</atitle><jtitle>Journal of plant pathology</jtitle><date>2008-03-01</date><risdate>2008</risdate><volume>90</volume><issue>1</issue><spage>125</spage><epage>130</epage><pages>125-130</pages><issn>1125-4653</issn><eissn>2239-7264</eissn><abstract>Grapevines from six Chilean regions were surveyed for virus diseases and tested for the presence of the most important viruses. ELISA testing of 2535 samples and confirmatory RT-PCR of some ELISA-negative samples from symptomatic and symptomless vines gave the following infection rates: 6.36% for Grapevine fanleaf virus (GFLV); 4.67% for Grapevine leafroll associated virus 1 (GLRaV-1); 16.05% for Grapevine leafroll associated virus 2 (GLRaV-2); 6.41% for Grapevine leafroll associated virus 3 (GLRaV-3); 0.26% for Grapevine leafroll associated virus 7 (GLRaV-7); 14.99% for Grapevine fleck virus (GFkV); 5.57% for Grapevine virus A (GVA) and 0.78% for Grapevine virus B (GVB). Strawberry latent ringspot virus (SLRSV) Tomato ringspot virus (ToRSV) and Arabis mosaic virus (ArMV) were not detected. Overall infection in the surveyed Chilean grapevines, considering ELISA and RT-PCR, was 32.35%. Virus infection ratio obtained from ELISA analysis in the six regions, varied between 21.19% (Región Metropolitana) and 74.26% (Coquimbo). RT-PCR was used for detection of the Red Globe strain of GLRaV-2 and Grapevine rupestris stem pitting associated virus (GRSPaV), and to confirm and extend ELISA results. GVB, GFkV, GRSPaV, GLRaV-2 RG and GLRaV-7 are new records for Chile.</abstract><pub>An International Journal of the Italian Phytopathological Society</pub><tpages>6</tpages></addata></record> |
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subjects | Arabis mosaic virus Grapevine fanleaf virus Grapevine fleck virus Grapevine virus A Grapevine virus B Infections Mosaic viruses Phytopathology Plant diseases Plants Reverse transcriptase polymerase chain reaction Rootstocks Satellite viruses Short Communication Strawberry latent ringspot virus Tomato ringspot virus Vineyards Viruses Vitaceae |
title | SURVEY OF GRAPEVINE VIRUSES IN CHILE |
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