Generation of a new strain of NOD/SCID/IL2Rγ -/- mice with targeted disruption of Prkdc and IL2Rγ genes using CRISPR/Cas9 system
The NOD/SCID/IL2Rγ (NSG) mouse strain is the most widely used immunodeficient strain for xenograft transplantation. However, the existing SCID mutation is a spontaneous mutation of the Prkdc gene, which leads to leaky T cell developmental block and difficulty in genotyping. It is therefore important...
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| Veröffentlicht in: | Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2018-06, Vol.38 (6), p.639-646 |
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| container_title | Nan fang yi ke da xue xue bao = Journal of Southern Medical University |
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| creator | Liu, Ya-Chen Chen, Qu Yang, Xing-Long Tang, Qing-Shuang Yao, Kai-Tai Xu, Yang |
| description | The NOD/SCID/IL2Rγ
(NSG) mouse strain is the most widely used immunodeficient strain for xenograft transplantation. However, the existing SCID mutation is a spontaneous mutation of the Prkdc gene, which leads to leaky T cell developmental block and difficulty in genotyping. It is therefore important to develop a new strain of NSG mice with targeted disruption of Prkdc and IL2Rγ genes.
Targeted disruption of Prkdc and IL2Rγ genes was achieved using the CRISPR/Cas9 system. By intercrossing the knockout and NOD mice, we obtained a novel strain of NOD/SCID/IL2Rγ
(NSG) mice, denoted as cNSG (Chinese NSG) mice.
In addition to the NOD mutation, cNSG mice exhibited a complete absence of T cells, B cells and NK cells. cNSG mice allowed more efficient engraftment of human cancer cells than the commonly used immunodeficient nude mice.
cNSG mice will provide an important xenotransplantation model for biomedical research. |
| format | Article |
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(NSG) mouse strain is the most widely used immunodeficient strain for xenograft transplantation. However, the existing SCID mutation is a spontaneous mutation of the Prkdc gene, which leads to leaky T cell developmental block and difficulty in genotyping. It is therefore important to develop a new strain of NSG mice with targeted disruption of Prkdc and IL2Rγ genes.
Targeted disruption of Prkdc and IL2Rγ genes was achieved using the CRISPR/Cas9 system. By intercrossing the knockout and NOD mice, we obtained a novel strain of NOD/SCID/IL2Rγ
(NSG) mice, denoted as cNSG (Chinese NSG) mice.
In addition to the NOD mutation, cNSG mice exhibited a complete absence of T cells, B cells and NK cells. cNSG mice allowed more efficient engraftment of human cancer cells than the commonly used immunodeficient nude mice.
cNSG mice will provide an important xenotransplantation model for biomedical research.</description><identifier>ISSN: 1673-4254</identifier><identifier>PMID: 29997084</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; B-Lymphocytes ; CRISPR-Cas Systems ; DNA-Activated Protein Kinase - genetics ; DNA-Binding Proteins - genetics ; Interleukin Receptor Common gamma Subunit - genetics ; Killer Cells, Natural ; Mice ; Mice, Inbred NOD - genetics ; Mice, Knockout ; Mice, Nude ; Mice, SCID - genetics ; Models, Animal ; Nuclear Proteins - genetics ; Selective Breeding - genetics ; Species Specificity ; T-Lymphocytes ; Transplantation, Heterologous</subject><ispartof>Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2018-06, Vol.38 (6), p.639-646</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29997084$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Ya-Chen</creatorcontrib><creatorcontrib>Chen, Qu</creatorcontrib><creatorcontrib>Yang, Xing-Long</creatorcontrib><creatorcontrib>Tang, Qing-Shuang</creatorcontrib><creatorcontrib>Yao, Kai-Tai</creatorcontrib><creatorcontrib>Xu, Yang</creatorcontrib><title>Generation of a new strain of NOD/SCID/IL2Rγ -/- mice with targeted disruption of Prkdc and IL2Rγ genes using CRISPR/Cas9 system</title><title>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</title><addtitle>Nan Fang Yi Ke Da Xue Xue Bao</addtitle><description>The NOD/SCID/IL2Rγ
(NSG) mouse strain is the most widely used immunodeficient strain for xenograft transplantation. However, the existing SCID mutation is a spontaneous mutation of the Prkdc gene, which leads to leaky T cell developmental block and difficulty in genotyping. It is therefore important to develop a new strain of NSG mice with targeted disruption of Prkdc and IL2Rγ genes.
Targeted disruption of Prkdc and IL2Rγ genes was achieved using the CRISPR/Cas9 system. By intercrossing the knockout and NOD mice, we obtained a novel strain of NOD/SCID/IL2Rγ
(NSG) mice, denoted as cNSG (Chinese NSG) mice.
In addition to the NOD mutation, cNSG mice exhibited a complete absence of T cells, B cells and NK cells. cNSG mice allowed more efficient engraftment of human cancer cells than the commonly used immunodeficient nude mice.
cNSG mice will provide an important xenotransplantation model for biomedical research.</description><subject>Animals</subject><subject>B-Lymphocytes</subject><subject>CRISPR-Cas Systems</subject><subject>DNA-Activated Protein Kinase - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Interleukin Receptor Common gamma Subunit - genetics</subject><subject>Killer Cells, Natural</subject><subject>Mice</subject><subject>Mice, Inbred NOD - genetics</subject><subject>Mice, Knockout</subject><subject>Mice, Nude</subject><subject>Mice, SCID - genetics</subject><subject>Models, Animal</subject><subject>Nuclear Proteins - genetics</subject><subject>Selective Breeding - genetics</subject><subject>Species Specificity</subject><subject>T-Lymphocytes</subject><subject>Transplantation, Heterologous</subject><issn>1673-4254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE1OwzAQRrMA0ar0CshLNlEcJ3biJUqhRKpo1cI6miTjYtH8YDuquu2VuAdnokC7Gn3S-95o5sobhyKJ_JjxeORNrdUl5WGUUC7ojTdiUsqEpvHYO86xRQNOdy3pFAHS4p5YZ0D_5ZflLNhk-SzIF2z9_UX8wCeNrpDstXsnDswWHdak1tYM_UWyMh91RaCtybm1Pe2wZLC63ZJsnW9W6yADK4k9WIfNrXetYGdxep4T7-3p8TV79hfLeZ49LPw-ZML5yDkDSCoJv0eUFVQJ8ljUKmQcQ6FoSaNUMS6QRTSuSkRJI0UVJhBKSCGaePf_3t50nwNaVzTaVrjbQYvdYAtGRSrD-GQ_oXdndCgbrIve6AbMobj8LfoBOglpHw</recordid><startdate>20180620</startdate><enddate>20180620</enddate><creator>Liu, Ya-Chen</creator><creator>Chen, Qu</creator><creator>Yang, Xing-Long</creator><creator>Tang, Qing-Shuang</creator><creator>Yao, Kai-Tai</creator><creator>Xu, Yang</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20180620</creationdate><title>Generation of a new strain of NOD/SCID/IL2Rγ -/- mice with targeted disruption of Prkdc and IL2Rγ genes using CRISPR/Cas9 system</title><author>Liu, Ya-Chen ; Chen, Qu ; Yang, Xing-Long ; Tang, Qing-Shuang ; Yao, Kai-Tai ; Xu, Yang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-e552aa7c9a0560bcac7e546df125e16f0b038f256e2304cbee903f0fe7a19a8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2018</creationdate><topic>Animals</topic><topic>B-Lymphocytes</topic><topic>CRISPR-Cas Systems</topic><topic>DNA-Activated Protein Kinase - genetics</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Interleukin Receptor Common gamma Subunit - genetics</topic><topic>Killer Cells, Natural</topic><topic>Mice</topic><topic>Mice, Inbred NOD - genetics</topic><topic>Mice, Knockout</topic><topic>Mice, Nude</topic><topic>Mice, SCID - genetics</topic><topic>Models, Animal</topic><topic>Nuclear Proteins - genetics</topic><topic>Selective Breeding - genetics</topic><topic>Species Specificity</topic><topic>T-Lymphocytes</topic><topic>Transplantation, Heterologous</topic><toplevel>online_resources</toplevel><creatorcontrib>Liu, Ya-Chen</creatorcontrib><creatorcontrib>Chen, Qu</creatorcontrib><creatorcontrib>Yang, Xing-Long</creatorcontrib><creatorcontrib>Tang, Qing-Shuang</creatorcontrib><creatorcontrib>Yao, Kai-Tai</creatorcontrib><creatorcontrib>Xu, Yang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Ya-Chen</au><au>Chen, Qu</au><au>Yang, Xing-Long</au><au>Tang, Qing-Shuang</au><au>Yao, Kai-Tai</au><au>Xu, Yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of a new strain of NOD/SCID/IL2Rγ -/- mice with targeted disruption of Prkdc and IL2Rγ genes using CRISPR/Cas9 system</atitle><jtitle>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</jtitle><addtitle>Nan Fang Yi Ke Da Xue Xue Bao</addtitle><date>2018-06-20</date><risdate>2018</risdate><volume>38</volume><issue>6</issue><spage>639</spage><epage>646</epage><pages>639-646</pages><issn>1673-4254</issn><abstract>The NOD/SCID/IL2Rγ
(NSG) mouse strain is the most widely used immunodeficient strain for xenograft transplantation. However, the existing SCID mutation is a spontaneous mutation of the Prkdc gene, which leads to leaky T cell developmental block and difficulty in genotyping. It is therefore important to develop a new strain of NSG mice with targeted disruption of Prkdc and IL2Rγ genes.
Targeted disruption of Prkdc and IL2Rγ genes was achieved using the CRISPR/Cas9 system. By intercrossing the knockout and NOD mice, we obtained a novel strain of NOD/SCID/IL2Rγ
(NSG) mice, denoted as cNSG (Chinese NSG) mice.
In addition to the NOD mutation, cNSG mice exhibited a complete absence of T cells, B cells and NK cells. cNSG mice allowed more efficient engraftment of human cancer cells than the commonly used immunodeficient nude mice.
cNSG mice will provide an important xenotransplantation model for biomedical research.</abstract><cop>China</cop><pmid>29997084</pmid><tpages>8</tpages></addata></record> |
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| ispartof | Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2018-06, Vol.38 (6), p.639-646 |
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| source | MEDLINE; PubMed Central |
| subjects | Animals B-Lymphocytes CRISPR-Cas Systems DNA-Activated Protein Kinase - genetics DNA-Binding Proteins - genetics Interleukin Receptor Common gamma Subunit - genetics Killer Cells, Natural Mice Mice, Inbred NOD - genetics Mice, Knockout Mice, Nude Mice, SCID - genetics Models, Animal Nuclear Proteins - genetics Selective Breeding - genetics Species Specificity T-Lymphocytes Transplantation, Heterologous |
| title | Generation of a new strain of NOD/SCID/IL2Rγ -/- mice with targeted disruption of Prkdc and IL2Rγ genes using CRISPR/Cas9 system |
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