A complementary forensic ‘proteo-genomic’ approach for the direct identification of biological fluid traces under fingernails
Violent contact between individuals during a crime can result in body fluids becoming trapped under the fingernails of the individuals involved. The traces under fingernails represent valuable forensic evidence because DNA profiling can indicate from whom the trace originated and proteomic methods c...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2018-09, Vol.410 (24), p.6165-6175 |
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| creator | Kamanna, Sathisha Henry, Julianne Voelcker, Nico Linacre, Adrian Paul Kirkbride, K. |
| description | Violent contact between individuals during a crime can result in body fluids becoming trapped under the fingernails of the individuals involved. The traces under fingernails represent valuable forensic evidence because DNA profiling can indicate from whom the trace originated and proteomic methods can be used to determine the type of fluid in the trace, thus providing evidence as to the circumstances surrounding the crime. Here, we present an initial study of an analytical strategy that involves two complementary techniques, direct PCR DNA profiling and direct mass spectrometry-based protein biomarker detection, for the comprehensive examination of traces of biological fluids gathered from underneath fingernails. With regard to protein biomarker detection, direct MALDI-ToF MS/MS is very sensitive, allowing results to be obtained from biological material present on only a few fibres plucked from a microswab used to collect the traces. Human cornulin, a protein biomarker for vaginal fluid, could be detected up to 5 h after it had been deposited under fingernails whereas haemoglobin, a biomarker for blood, is somewhat more persistent under fingernails and could be detected up to 18 h post-deposition. Bottom-up tandem mass spectrometry techniques were used to provide a high level of confidence in assigning the identity of protein biomarkers. nLC-ESI-qToF MS/MS offered higher levels of confidence and the ability to detect traces that had been present under fingernails for longer periods of time, but this performance came with the cost of longer analysis time and a more laborious sampling approach.
Graphical abstract
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| doi_str_mv | 10.1007/s00216-018-1223-3 |
| format | Article |
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Graphical abstract
ᅟ</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-018-1223-3</identifier><identifier>PMID: 29998367</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analytical Chemistry ; Biochemistry ; Biological materials ; Biomarkers ; Body fluids ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Cost analysis ; Crime ; Deoxyribonucleic acid ; DNA ; DNA fingerprinting ; Fibers ; Fingernails ; Fluids ; Food Science ; Forensic engineering ; Forensic genetics ; Forensic science ; Forensic sciences ; Genetic aspects ; Hemoglobin ; Laboratory Medicine ; Mass spectrometry ; Mass spectroscopy ; Methods ; Monitoring/Environmental Analysis ; Proteins ; Research Paper ; Scientific imaging ; Spectroscopy ; Vagina</subject><ispartof>Analytical and bioanalytical chemistry, 2018-09, Vol.410 (24), p.6165-6175</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-9f817365935265cefd4ae8fa99b1d9ae61d400a3672225fe480cb22562d6e4f83</citedby><cites>FETCH-LOGICAL-c476t-9f817365935265cefd4ae8fa99b1d9ae61d400a3672225fe480cb22562d6e4f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-018-1223-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-018-1223-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29998367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kamanna, Sathisha</creatorcontrib><creatorcontrib>Henry, Julianne</creatorcontrib><creatorcontrib>Voelcker, Nico</creatorcontrib><creatorcontrib>Linacre, Adrian</creatorcontrib><creatorcontrib>Paul Kirkbride, K.</creatorcontrib><title>A complementary forensic ‘proteo-genomic’ approach for the direct identification of biological fluid traces under fingernails</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Violent contact between individuals during a crime can result in body fluids becoming trapped under the fingernails of the individuals involved. The traces under fingernails represent valuable forensic evidence because DNA profiling can indicate from whom the trace originated and proteomic methods can be used to determine the type of fluid in the trace, thus providing evidence as to the circumstances surrounding the crime. Here, we present an initial study of an analytical strategy that involves two complementary techniques, direct PCR DNA profiling and direct mass spectrometry-based protein biomarker detection, for the comprehensive examination of traces of biological fluids gathered from underneath fingernails. With regard to protein biomarker detection, direct MALDI-ToF MS/MS is very sensitive, allowing results to be obtained from biological material present on only a few fibres plucked from a microswab used to collect the traces. Human cornulin, a protein biomarker for vaginal fluid, could be detected up to 5 h after it had been deposited under fingernails whereas haemoglobin, a biomarker for blood, is somewhat more persistent under fingernails and could be detected up to 18 h post-deposition. Bottom-up tandem mass spectrometry techniques were used to provide a high level of confidence in assigning the identity of protein biomarkers. nLC-ESI-qToF MS/MS offered higher levels of confidence and the ability to detect traces that had been present under fingernails for longer periods of time, but this performance came with the cost of longer analysis time and a more laborious sampling approach.
Graphical abstract
ᅟ</description><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Biological materials</subject><subject>Biomarkers</subject><subject>Body fluids</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cost analysis</subject><subject>Crime</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA fingerprinting</subject><subject>Fibers</subject><subject>Fingernails</subject><subject>Fluids</subject><subject>Food Science</subject><subject>Forensic engineering</subject><subject>Forensic genetics</subject><subject>Forensic science</subject><subject>Forensic sciences</subject><subject>Genetic aspects</subject><subject>Hemoglobin</subject><subject>Laboratory Medicine</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Methods</subject><subject>Monitoring/Environmental 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fingernails</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2018-09-01</date><risdate>2018</risdate><volume>410</volume><issue>24</issue><spage>6165</spage><epage>6175</epage><pages>6165-6175</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Violent contact between individuals during a crime can result in body fluids becoming trapped under the fingernails of the individuals involved. The traces under fingernails represent valuable forensic evidence because DNA profiling can indicate from whom the trace originated and proteomic methods can be used to determine the type of fluid in the trace, thus providing evidence as to the circumstances surrounding the crime. Here, we present an initial study of an analytical strategy that involves two complementary techniques, direct PCR DNA profiling and direct mass spectrometry-based protein biomarker detection, for the comprehensive examination of traces of biological fluids gathered from underneath fingernails. With regard to protein biomarker detection, direct MALDI-ToF MS/MS is very sensitive, allowing results to be obtained from biological material present on only a few fibres plucked from a microswab used to collect the traces. Human cornulin, a protein biomarker for vaginal fluid, could be detected up to 5 h after it had been deposited under fingernails whereas haemoglobin, a biomarker for blood, is somewhat more persistent under fingernails and could be detected up to 18 h post-deposition. Bottom-up tandem mass spectrometry techniques were used to provide a high level of confidence in assigning the identity of protein biomarkers. nLC-ESI-qToF MS/MS offered higher levels of confidence and the ability to detect traces that had been present under fingernails for longer periods of time, but this performance came with the cost of longer analysis time and a more laborious sampling approach.
Graphical abstract
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| subjects | Analytical Chemistry Biochemistry Biological materials Biomarkers Body fluids Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Cost analysis Crime Deoxyribonucleic acid DNA DNA fingerprinting Fibers Fingernails Fluids Food Science Forensic engineering Forensic genetics Forensic science Forensic sciences Genetic aspects Hemoglobin Laboratory Medicine Mass spectrometry Mass spectroscopy Methods Monitoring/Environmental Analysis Proteins Research Paper Scientific imaging Spectroscopy Vagina |
| title | A complementary forensic ‘proteo-genomic’ approach for the direct identification of biological fluid traces under fingernails |
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