FabG Mediates Polyhydroxyalkanoate Production from Both Related and Nonrelated Carbon Sources in Recombinant Escherichia coli LS5218

FabG Mediates Polyhydroxyalkanoate Production from Both Related and Nonrelated Carbon Sources in Recombinant Escherichia coli LS5218

Polyhydroxyalkanoates (PHAs) composed of a mixture of short-chain-length- medium-chain-length (SCL-MCL) hydroxyacyl monomers are biologically produced polyesters that have properties ranging from thermoplastic to elastomeric, dependent on the molar ratio of SCL to MCL monomers incorporated into the...

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Veröffentlicht in:Biotechnology progress 2008-01, Vol.24 (2), p.342-351
Hauptverfasser: Nomura, Christopher T, Tanaka, Tomoyo, Eguen, Tenai E, Appah, Alexandria S, Matsumoto, Ken'ichiro, Taguchi, Seiichi, Ortiz, CLeo, Doi, Yoshiharu
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Escherichia coli
Pseudomonas
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container_end_page 351
container_issue 2
container_start_page 342
container_title Biotechnology progress
container_volume 24
creator Nomura, Christopher T
Tanaka, Tomoyo
Eguen, Tenai E
Appah, Alexandria S
Matsumoto, Ken'ichiro
Taguchi, Seiichi
Ortiz, CLeo
Doi, Yoshiharu
description Polyhydroxyalkanoates (PHAs) composed of a mixture of short-chain-length- medium-chain-length (SCL-MCL) hydroxyacyl monomers are biologically produced polyesters that have properties ranging from thermoplastic to elastomeric, dependent on the molar ratio of SCL to MCL monomers incorporated into the copolymer. Because of the potential wide range of properties and applications for SCL-MCL PHA copolymers, it is important to develop and characterize novel metabolic pathways for SCL-MCL PHA production. The current study shows that coexpression of fabG genes from either E. coli or Pseudomonas sp. 61-3 with fabH(F87T) and PHA synthase genes enhances the production of SCL-MCL PHA copolymer from both related and nonrelated carbon sources in Escherichia coli LS5218, indicating the flexibility of FabG as a monomer- supplying enzyme for biological PHA production.
doi_str_mv 10.1021/bp070303yPII:S8756-7938(07)00303-7
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subjects Escherichia coli
Pseudomonas
title FabG Mediates Polyhydroxyalkanoate Production from Both Related and Nonrelated Carbon Sources in Recombinant Escherichia coli LS5218
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