Salivary and Serum Anti-Desmoglein 1 and 3 ELISA and Indirect Immunofluorescence in Pemphigus Vulgaris: Correlations with Serum ELISA, Indirect Immunofluorescence and Disease Severity
Anti-desmoglein (anti-Dsg) ELISA and indirect immunofluorescence (IIF) are used for the diagnosis of pemphigus vulgaris (PV). The value of salivary ELISA, serum ELISA, and IIF in the diagnosis of PV, and the correlation of salivary anti-Dsg1 and anti-Dsg3 ELISA with serum ELISA, serum and salivary I...
Gespeichert in:
| Veröffentlicht in: | Acta dermatovenerologica Croatica 2018-06, Vol.26 (2), p.91-99 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 99 |
|---|---|
| container_issue | 2 |
| container_start_page | 91 |
| container_title | Acta dermatovenerologica Croatica |
| container_volume | 26 |
| creator | Koopai, Maryam Mortazavi, Hossein Khatami, Alireza Khodashenas, Zohreh |
| description | Anti-desmoglein (anti-Dsg) ELISA and indirect immunofluorescence (IIF) are used for the diagnosis of pemphigus vulgaris (PV). The value of salivary ELISA, serum ELISA, and IIF in the diagnosis of PV, and the correlation of salivary anti-Dsg1 and anti-Dsg3 ELISA with serum ELISA, serum and salivary IIF titers, and disease severity in patients with PV were evaluated. Fifty newly diagnosed patients with PV were enrolled in the study. Demographic data and disease-severity scores were recorded for each patient. Anti-Dsg1 and anti-Dsg3 ELISA and IIF were performed on both serum and salivary samples. Given the cut-off value of 20 RU/mL for Dsg1 and Dsg3, serum Dsg1 and Dsg3 ELISA were positive in 41 (82%) and 40 (80%) patients, and saliva Dsg1 and the Dsg3 ELISA were positive in 2 (4%) and 3 (6%) patients, respectively. Using the cut-off value of 13.4 RU/mL and 7.7 RU/mL for Dsg3 and Dsg1 salivary ELISA, 25 (50%) and 23 (46%) patients tested positive for Dsg3 and Dsg1, respectively. Serum IIF results were positive in 35 (70%) patients, and salivary IIF results were positive in 16 (32%) patients. Salivary anti-Dsg1 and anti-Dsg3 showed moderate correlations with the total pemphigus disease area index (PDAI) score (r=0.466, P |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_2068352087</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2068352087</sourcerecordid><originalsourceid>FETCH-LOGICAL-p211t-40d1b0e86526dd5a83b133f695caed8351220fc129af6338b558eab0ec9078093</originalsourceid><addsrcrecordid>eNp9kN1KwzAUx4sgbk5fQXLphYV8LG3q3djmLAwUpt6WtD3dIklak2ayJ_P1rHPeenUOnP_H4XcWjYmYpnHCmRhFl96_Y5yIlJKLaESzTGQiYePoayO12kt3QNLWaAMuGDSzvYoX4E271aAsIscbQ8t1vpkd99zWykHVo9yYYNtGh9aBr8BWgAbDM5hup7bBo7egt9Ipf4_mrXOgZa9a69Gn6nensmPo3b-JP40L5UF6GEx7cKo_XEXnjdQerk9zEr0-LF_mj_H6aZXPZ-u4o4T08RTXpMQgEk6TuuZSsJIw1iQZryTUgnFCKW4qQjPZJIyJknMBcnBUGU4Fztgkuv3N7Vz7EcD3hVHDW1pLC23wBR2YMk6xSAfpzUkaSgN10TllBrDFH2z2DQE-fGI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2068352087</pqid></control><display><type>article</type><title>Salivary and Serum Anti-Desmoglein 1 and 3 ELISA and Indirect Immunofluorescence in Pemphigus Vulgaris: Correlations with Serum ELISA, Indirect Immunofluorescence and Disease Severity</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Koopai, Maryam ; Mortazavi, Hossein ; Khatami, Alireza ; Khodashenas, Zohreh</creator><creatorcontrib>Koopai, Maryam ; Mortazavi, Hossein ; Khatami, Alireza ; Khodashenas, Zohreh</creatorcontrib><description>Anti-desmoglein (anti-Dsg) ELISA and indirect immunofluorescence (IIF) are used for the diagnosis of pemphigus vulgaris (PV). The value of salivary ELISA, serum ELISA, and IIF in the diagnosis of PV, and the correlation of salivary anti-Dsg1 and anti-Dsg3 ELISA with serum ELISA, serum and salivary IIF titers, and disease severity in patients with PV were evaluated. Fifty newly diagnosed patients with PV were enrolled in the study. Demographic data and disease-severity scores were recorded for each patient. Anti-Dsg1 and anti-Dsg3 ELISA and IIF were performed on both serum and salivary samples. Given the cut-off value of 20 RU/mL for Dsg1 and Dsg3, serum Dsg1 and Dsg3 ELISA were positive in 41 (82%) and 40 (80%) patients, and saliva Dsg1 and the Dsg3 ELISA were positive in 2 (4%) and 3 (6%) patients, respectively. Using the cut-off value of 13.4 RU/mL and 7.7 RU/mL for Dsg3 and Dsg1 salivary ELISA, 25 (50%) and 23 (46%) patients tested positive for Dsg3 and Dsg1, respectively. Serum IIF results were positive in 35 (70%) patients, and salivary IIF results were positive in 16 (32%) patients. Salivary anti-Dsg1 and anti-Dsg3 showed moderate correlations with the total pemphigus disease area index (PDAI) score (r=0.466, P<0.001), (r=0.459, P<0.001), respectively. A moderate correlation between serum IIF and salivary IIF was also detected (r=0.413, P<0.001). Salivary anti-Dsg1 and anti-Dsg3 ELISA could be used as a safe and noninvasive method for the diagnosis of PV under certain circumstances, especially in children or elderly patients. Salivary ELISA is superior to salivary IIF.</description><identifier>EISSN: 1847-6538</identifier><identifier>PMID: 29989863</identifier><language>eng</language><publisher>Croatia</publisher><subject>Adult ; Aged ; Autoantibodies - metabolism ; Cross-Sectional Studies ; Desmoglein 1 - immunology ; Desmoglein 3 - immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Fluorescent Antibody Technique, Indirect ; Humans ; Male ; Middle Aged ; Pemphigus - diagnosis ; Pemphigus - immunology ; Pemphigus - metabolism ; Predictive Value of Tests ; Saliva - immunology ; Severity of Illness Index ; Young Adult</subject><ispartof>Acta dermatovenerologica Croatica, 2018-06, Vol.26 (2), p.91-99</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29989863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koopai, Maryam</creatorcontrib><creatorcontrib>Mortazavi, Hossein</creatorcontrib><creatorcontrib>Khatami, Alireza</creatorcontrib><creatorcontrib>Khodashenas, Zohreh</creatorcontrib><title>Salivary and Serum Anti-Desmoglein 1 and 3 ELISA and Indirect Immunofluorescence in Pemphigus Vulgaris: Correlations with Serum ELISA, Indirect Immunofluorescence and Disease Severity</title><title>Acta dermatovenerologica Croatica</title><addtitle>Acta Dermatovenerol Croat</addtitle><description>Anti-desmoglein (anti-Dsg) ELISA and indirect immunofluorescence (IIF) are used for the diagnosis of pemphigus vulgaris (PV). The value of salivary ELISA, serum ELISA, and IIF in the diagnosis of PV, and the correlation of salivary anti-Dsg1 and anti-Dsg3 ELISA with serum ELISA, serum and salivary IIF titers, and disease severity in patients with PV were evaluated. Fifty newly diagnosed patients with PV were enrolled in the study. Demographic data and disease-severity scores were recorded for each patient. Anti-Dsg1 and anti-Dsg3 ELISA and IIF were performed on both serum and salivary samples. Given the cut-off value of 20 RU/mL for Dsg1 and Dsg3, serum Dsg1 and Dsg3 ELISA were positive in 41 (82%) and 40 (80%) patients, and saliva Dsg1 and the Dsg3 ELISA were positive in 2 (4%) and 3 (6%) patients, respectively. Using the cut-off value of 13.4 RU/mL and 7.7 RU/mL for Dsg3 and Dsg1 salivary ELISA, 25 (50%) and 23 (46%) patients tested positive for Dsg3 and Dsg1, respectively. Serum IIF results were positive in 35 (70%) patients, and salivary IIF results were positive in 16 (32%) patients. Salivary anti-Dsg1 and anti-Dsg3 showed moderate correlations with the total pemphigus disease area index (PDAI) score (r=0.466, P<0.001), (r=0.459, P<0.001), respectively. A moderate correlation between serum IIF and salivary IIF was also detected (r=0.413, P<0.001). Salivary anti-Dsg1 and anti-Dsg3 ELISA could be used as a safe and noninvasive method for the diagnosis of PV under certain circumstances, especially in children or elderly patients. Salivary ELISA is superior to salivary IIF.</description><subject>Adult</subject><subject>Aged</subject><subject>Autoantibodies - metabolism</subject><subject>Cross-Sectional Studies</subject><subject>Desmoglein 1 - immunology</subject><subject>Desmoglein 3 - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Pemphigus - diagnosis</subject><subject>Pemphigus - immunology</subject><subject>Pemphigus - metabolism</subject><subject>Predictive Value of Tests</subject><subject>Saliva - immunology</subject><subject>Severity of Illness Index</subject><subject>Young Adult</subject><issn>1847-6538</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kN1KwzAUx4sgbk5fQXLphYV8LG3q3djmLAwUpt6WtD3dIklak2ayJ_P1rHPeenUOnP_H4XcWjYmYpnHCmRhFl96_Y5yIlJKLaESzTGQiYePoayO12kt3QNLWaAMuGDSzvYoX4E271aAsIscbQ8t1vpkd99zWykHVo9yYYNtGh9aBr8BWgAbDM5hup7bBo7egt9Ipf4_mrXOgZa9a69Gn6nensmPo3b-JP40L5UF6GEx7cKo_XEXnjdQerk9zEr0-LF_mj_H6aZXPZ-u4o4T08RTXpMQgEk6TuuZSsJIw1iQZryTUgnFCKW4qQjPZJIyJknMBcnBUGU4Fztgkuv3N7Vz7EcD3hVHDW1pLC23wBR2YMk6xSAfpzUkaSgN10TllBrDFH2z2DQE-fGI</recordid><startdate>201806</startdate><enddate>201806</enddate><creator>Koopai, Maryam</creator><creator>Mortazavi, Hossein</creator><creator>Khatami, Alireza</creator><creator>Khodashenas, Zohreh</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201806</creationdate><title>Salivary and Serum Anti-Desmoglein 1 and 3 ELISA and Indirect Immunofluorescence in Pemphigus Vulgaris: Correlations with Serum ELISA, Indirect Immunofluorescence and Disease Severity</title><author>Koopai, Maryam ; Mortazavi, Hossein ; Khatami, Alireza ; Khodashenas, Zohreh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-40d1b0e86526dd5a83b133f695caed8351220fc129af6338b558eab0ec9078093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Autoantibodies - metabolism</topic><topic>Cross-Sectional Studies</topic><topic>Desmoglein 1 - immunology</topic><topic>Desmoglein 3 - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Pemphigus - diagnosis</topic><topic>Pemphigus - immunology</topic><topic>Pemphigus - metabolism</topic><topic>Predictive Value of Tests</topic><topic>Saliva - immunology</topic><topic>Severity of Illness Index</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koopai, Maryam</creatorcontrib><creatorcontrib>Mortazavi, Hossein</creatorcontrib><creatorcontrib>Khatami, Alireza</creatorcontrib><creatorcontrib>Khodashenas, Zohreh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Acta dermatovenerologica Croatica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koopai, Maryam</au><au>Mortazavi, Hossein</au><au>Khatami, Alireza</au><au>Khodashenas, Zohreh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Salivary and Serum Anti-Desmoglein 1 and 3 ELISA and Indirect Immunofluorescence in Pemphigus Vulgaris: Correlations with Serum ELISA, Indirect Immunofluorescence and Disease Severity</atitle><jtitle>Acta dermatovenerologica Croatica</jtitle><addtitle>Acta Dermatovenerol Croat</addtitle><date>2018-06</date><risdate>2018</risdate><volume>26</volume><issue>2</issue><spage>91</spage><epage>99</epage><pages>91-99</pages><eissn>1847-6538</eissn><abstract>Anti-desmoglein (anti-Dsg) ELISA and indirect immunofluorescence (IIF) are used for the diagnosis of pemphigus vulgaris (PV). The value of salivary ELISA, serum ELISA, and IIF in the diagnosis of PV, and the correlation of salivary anti-Dsg1 and anti-Dsg3 ELISA with serum ELISA, serum and salivary IIF titers, and disease severity in patients with PV were evaluated. Fifty newly diagnosed patients with PV were enrolled in the study. Demographic data and disease-severity scores were recorded for each patient. Anti-Dsg1 and anti-Dsg3 ELISA and IIF were performed on both serum and salivary samples. Given the cut-off value of 20 RU/mL for Dsg1 and Dsg3, serum Dsg1 and Dsg3 ELISA were positive in 41 (82%) and 40 (80%) patients, and saliva Dsg1 and the Dsg3 ELISA were positive in 2 (4%) and 3 (6%) patients, respectively. Using the cut-off value of 13.4 RU/mL and 7.7 RU/mL for Dsg3 and Dsg1 salivary ELISA, 25 (50%) and 23 (46%) patients tested positive for Dsg3 and Dsg1, respectively. Serum IIF results were positive in 35 (70%) patients, and salivary IIF results were positive in 16 (32%) patients. Salivary anti-Dsg1 and anti-Dsg3 showed moderate correlations with the total pemphigus disease area index (PDAI) score (r=0.466, P<0.001), (r=0.459, P<0.001), respectively. A moderate correlation between serum IIF and salivary IIF was also detected (r=0.413, P<0.001). Salivary anti-Dsg1 and anti-Dsg3 ELISA could be used as a safe and noninvasive method for the diagnosis of PV under certain circumstances, especially in children or elderly patients. Salivary ELISA is superior to salivary IIF.</abstract><cop>Croatia</cop><pmid>29989863</pmid><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1847-6538 |
| ispartof | Acta dermatovenerologica Croatica, 2018-06, Vol.26 (2), p.91-99 |
| issn | 1847-6538 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2068352087 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adult Aged Autoantibodies - metabolism Cross-Sectional Studies Desmoglein 1 - immunology Desmoglein 3 - immunology Enzyme-Linked Immunosorbent Assay Female Fluorescent Antibody Technique, Indirect Humans Male Middle Aged Pemphigus - diagnosis Pemphigus - immunology Pemphigus - metabolism Predictive Value of Tests Saliva - immunology Severity of Illness Index Young Adult |
| title | Salivary and Serum Anti-Desmoglein 1 and 3 ELISA and Indirect Immunofluorescence in Pemphigus Vulgaris: Correlations with Serum ELISA, Indirect Immunofluorescence and Disease Severity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T23%3A42%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Salivary%20and%20Serum%20Anti-Desmoglein%201%20and%203%20ELISA%20and%20Indirect%20Immunofluorescence%20in%20Pemphigus%20Vulgaris:%20Correlations%20with%20Serum%20ELISA,%20Indirect%20Immunofluorescence%20and%20Disease%20Severity&rft.jtitle=Acta%20dermatovenerologica%20Croatica&rft.au=Koopai,%20Maryam&rft.date=2018-06&rft.volume=26&rft.issue=2&rft.spage=91&rft.epage=99&rft.pages=91-99&rft.eissn=1847-6538&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E2068352087%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2068352087&rft_id=info:pmid/29989863&rfr_iscdi=true |