Hydrogen peroxide modulates redox status, energy metabolism, and gene expression in a dose‐ and time‐dependent manner in rat liver
Aim The aim of this study was to investigate the effect of three different hydrogen peroxide (H2O2) levels on blood and liver oxidative status, energy metabolites, and gene expression in male albino rats at two time intervals (2 and 4 weeks). Methods A total of 32 rats were divided into four groups....
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| Veröffentlicht in: | Journal of biochemical and molecular toxicology 2018-10, Vol.32 (10), p.e22199-N/A |
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| creator | Ahmed‐Farid, Omar A. Rizk, Hanan A. Shehata, Ahmed M. |
| description | Aim
The aim of this study was to investigate the effect of three different hydrogen peroxide (H2O2) levels on blood and liver oxidative status, energy metabolites, and gene expression in male albino rats at two time intervals (2 and 4 weeks).
Methods
A total of 32 rats were divided into four groups. The first group received tap water and served as control. The second group received low dose of hydrogen peroxide (H2O2; 0.25%), The third group received medium dose of H2O2 (0.5%) and the fourth group received high dose of H2O2 (1%) in drinking water.
Results
Present data showed that medium and high dose increased oxidative stress markers, decreased cell energy, and decreased antioxidant enzyme gene expression (GPx and Nrf2) and its downstream in contrast low dose did not show significant effects.
Conclusion
This study might indicate that hydrogen peroxide medium level is the best dose for redox model status. |
| doi_str_mv | 10.1002/jbt.22199 |
| format | Article |
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The aim of this study was to investigate the effect of three different hydrogen peroxide (H2O2) levels on blood and liver oxidative status, energy metabolites, and gene expression in male albino rats at two time intervals (2 and 4 weeks).
Methods
A total of 32 rats were divided into four groups. The first group received tap water and served as control. The second group received low dose of hydrogen peroxide (H2O2; 0.25%), The third group received medium dose of H2O2 (0.5%) and the fourth group received high dose of H2O2 (1%) in drinking water.
Results
Present data showed that medium and high dose increased oxidative stress markers, decreased cell energy, and decreased antioxidant enzyme gene expression (GPx and Nrf2) and its downstream in contrast low dose did not show significant effects.
Conclusion
This study might indicate that hydrogen peroxide medium level is the best dose for redox model status.</description><identifier>ISSN: 1095-6670</identifier><identifier>EISSN: 1099-0461</identifier><identifier>DOI: 10.1002/jbt.22199</identifier><identifier>PMID: 29992723</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Alanine Transaminase - blood ; Animals ; Antioxidants ; Aspartate Aminotransferases - blood ; Biomarkers - metabolism ; cell energy ; Deoxyguanosine - analogs & derivatives ; Deoxyguanosine - metabolism ; Dose-Response Relationship, Drug ; Drinking water ; Energy metabolism ; Energy Metabolism - drug effects ; Gene expression ; Gene Expression - drug effects ; Glutathione Peroxidase - metabolism ; Hydrogen ; Hydrogen peroxide ; Hydrogen Peroxide - administration & dosage ; Hydrogen Peroxide - toxicity ; Liver ; Liver - drug effects ; Liver - enzymology ; Liver - metabolism ; Male ; Malondialdehyde - metabolism ; Metabolism ; Metabolites ; NF-E2-Related Factor 2 - metabolism ; Nrf2 pathway ; NRF2 protein ; Oxidation-Reduction ; Oxidative Stress ; oxidative stress model ; Rats ; Rats, Sprague-Dawley ; Reactive Oxygen Species - metabolism ; Rodents ; Time dependence ; Time Factors</subject><ispartof>Journal of biochemical and molecular toxicology, 2018-10, Vol.32 (10), p.e22199-N/A</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-615de1ef8dfe65d4b6b74b722177a6c985bb26b2f98d89a35dbfd58cad6167ad3</citedby><cites>FETCH-LOGICAL-c3539-615de1ef8dfe65d4b6b74b722177a6c985bb26b2f98d89a35dbfd58cad6167ad3</cites><orcidid>0000-0002-1020-5777</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbt.22199$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbt.22199$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29992723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahmed‐Farid, Omar A.</creatorcontrib><creatorcontrib>Rizk, Hanan A.</creatorcontrib><creatorcontrib>Shehata, Ahmed M.</creatorcontrib><title>Hydrogen peroxide modulates redox status, energy metabolism, and gene expression in a dose‐ and time‐dependent manner in rat liver</title><title>Journal of biochemical and molecular toxicology</title><addtitle>J Biochem Mol Toxicol</addtitle><description>Aim
The aim of this study was to investigate the effect of three different hydrogen peroxide (H2O2) levels on blood and liver oxidative status, energy metabolites, and gene expression in male albino rats at two time intervals (2 and 4 weeks).
Methods
A total of 32 rats were divided into four groups. The first group received tap water and served as control. The second group received low dose of hydrogen peroxide (H2O2; 0.25%), The third group received medium dose of H2O2 (0.5%) and the fourth group received high dose of H2O2 (1%) in drinking water.
Results
Present data showed that medium and high dose increased oxidative stress markers, decreased cell energy, and decreased antioxidant enzyme gene expression (GPx and Nrf2) and its downstream in contrast low dose did not show significant effects.
Conclusion
This study might indicate that hydrogen peroxide medium level is the best dose for redox model status.</description><subject>Alanine Transaminase - blood</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Aspartate Aminotransferases - blood</subject><subject>Biomarkers - metabolism</subject><subject>cell energy</subject><subject>Deoxyguanosine - analogs & derivatives</subject><subject>Deoxyguanosine - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drinking water</subject><subject>Energy metabolism</subject><subject>Energy Metabolism - drug effects</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Hydrogen</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - administration & dosage</subject><subject>Hydrogen Peroxide - toxicity</subject><subject>Liver</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Malondialdehyde - metabolism</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>NF-E2-Related Factor 2 - metabolism</subject><subject>Nrf2 pathway</subject><subject>NRF2 protein</subject><subject>Oxidation-Reduction</subject><subject>Oxidative Stress</subject><subject>oxidative stress model</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Rodents</subject><subject>Time dependence</subject><subject>Time Factors</subject><issn>1095-6670</issn><issn>1099-0461</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10bFu1TAUBmALUdFSGHgBZIkFpKa1ndiJR6iAtqrEUmbLjk8qXyV2sJ1y79apM8_Ik-B7b8uAxOQzfOfXsX6E3lBySglhZyuTTxmjUj5DR5RIWZFG0Oe7mVdCtOQQvUxpRQjhsuUv0CGTUrKW1Ufo4WJjY7gFj2eIYe0s4CnYZdQZEo5gwxqnrPOSTjB4iLcbPEHWJowuTSdYe4vLLmBYzxFScsFj57HGNiT4ff9rB7KbtrOFGbwFn_GkfYnawqgzHt0dxFfoYNBjgteP7zH6_uXzzflFdf3t6-X5x-uqr3ktK0G5BQpDZwcQ3DZGmLYxbfl722rRy44bw4Rhg-xsJ3XNrRks73ptBRWttvUxer_PnWP4sUDKanKph3HUHsKSFCOiq5u64bLQd__QVViiL9cpRhnpGsprWtSHvepjSCnCoOboJh03ihK1LUeVctSunGLfPiYuZgL7Vz61UcDZHvx0I2z-n6SuPt3sI_8A1tmcjQ</recordid><startdate>201810</startdate><enddate>201810</enddate><creator>Ahmed‐Farid, Omar A.</creator><creator>Rizk, Hanan A.</creator><creator>Shehata, Ahmed M.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1020-5777</orcidid></search><sort><creationdate>201810</creationdate><title>Hydrogen peroxide modulates redox status, energy metabolism, and gene expression in a dose‐ and time‐dependent manner in rat liver</title><author>Ahmed‐Farid, Omar A. ; Rizk, Hanan A. ; Shehata, Ahmed M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-615de1ef8dfe65d4b6b74b722177a6c985bb26b2f98d89a35dbfd58cad6167ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alanine Transaminase - blood</topic><topic>Animals</topic><topic>Antioxidants</topic><topic>Aspartate Aminotransferases - blood</topic><topic>Biomarkers - metabolism</topic><topic>cell energy</topic><topic>Deoxyguanosine - analogs & derivatives</topic><topic>Deoxyguanosine - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drinking water</topic><topic>Energy metabolism</topic><topic>Energy Metabolism - drug effects</topic><topic>Gene expression</topic><topic>Gene Expression - drug effects</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Hydrogen</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - administration & dosage</topic><topic>Hydrogen Peroxide - toxicity</topic><topic>Liver</topic><topic>Liver - drug effects</topic><topic>Liver - enzymology</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Malondialdehyde - metabolism</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>NF-E2-Related Factor 2 - metabolism</topic><topic>Nrf2 pathway</topic><topic>NRF2 protein</topic><topic>Oxidation-Reduction</topic><topic>Oxidative Stress</topic><topic>oxidative stress model</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Rodents</topic><topic>Time dependence</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahmed‐Farid, Omar A.</creatorcontrib><creatorcontrib>Rizk, Hanan A.</creatorcontrib><creatorcontrib>Shehata, Ahmed M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemical and molecular toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahmed‐Farid, Omar A.</au><au>Rizk, Hanan A.</au><au>Shehata, Ahmed M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrogen peroxide modulates redox status, energy metabolism, and gene expression in a dose‐ and time‐dependent manner in rat liver</atitle><jtitle>Journal of biochemical and molecular toxicology</jtitle><addtitle>J Biochem Mol Toxicol</addtitle><date>2018-10</date><risdate>2018</risdate><volume>32</volume><issue>10</issue><spage>e22199</spage><epage>N/A</epage><pages>e22199-N/A</pages><issn>1095-6670</issn><eissn>1099-0461</eissn><abstract>Aim
The aim of this study was to investigate the effect of three different hydrogen peroxide (H2O2) levels on blood and liver oxidative status, energy metabolites, and gene expression in male albino rats at two time intervals (2 and 4 weeks).
Methods
A total of 32 rats were divided into four groups. The first group received tap water and served as control. The second group received low dose of hydrogen peroxide (H2O2; 0.25%), The third group received medium dose of H2O2 (0.5%) and the fourth group received high dose of H2O2 (1%) in drinking water.
Results
Present data showed that medium and high dose increased oxidative stress markers, decreased cell energy, and decreased antioxidant enzyme gene expression (GPx and Nrf2) and its downstream in contrast low dose did not show significant effects.
Conclusion
This study might indicate that hydrogen peroxide medium level is the best dose for redox model status.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29992723</pmid><doi>10.1002/jbt.22199</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-1020-5777</orcidid></addata></record> |
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| subjects | Alanine Transaminase - blood Animals Antioxidants Aspartate Aminotransferases - blood Biomarkers - metabolism cell energy Deoxyguanosine - analogs & derivatives Deoxyguanosine - metabolism Dose-Response Relationship, Drug Drinking water Energy metabolism Energy Metabolism - drug effects Gene expression Gene Expression - drug effects Glutathione Peroxidase - metabolism Hydrogen Hydrogen peroxide Hydrogen Peroxide - administration & dosage Hydrogen Peroxide - toxicity Liver Liver - drug effects Liver - enzymology Liver - metabolism Male Malondialdehyde - metabolism Metabolism Metabolites NF-E2-Related Factor 2 - metabolism Nrf2 pathway NRF2 protein Oxidation-Reduction Oxidative Stress oxidative stress model Rats Rats, Sprague-Dawley Reactive Oxygen Species - metabolism Rodents Time dependence Time Factors |
| title | Hydrogen peroxide modulates redox status, energy metabolism, and gene expression in a dose‐ and time‐dependent manner in rat liver |
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