Genome-wide C-SWAT library for high-throughput yeast genome tagging

Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. We validated the library with targeted sequencing a...

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Veröffentlicht in:	Nature methods 2018-08, Vol.15 (8), p.598-600
Hauptverfasser:	Meurer, Matthias, Duan, Yuanqiang, Sass, Ehud, Kats, Ilia, Herbst, Konrad, Buchmuller, Benjamin C., Dederer, Verena, Huber, Florian, Kirrmaier, Daniel, Štefl, Martin, Van Laer, Koen, Dick, Tobias P., Lemberg, Marius K., Khmelinskii, Anton, Levy, Emmanuel D., Knop, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1511 631/208 631/553 631/61/191 631/80 Baking yeast Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Brief Communication Fluorescence Genomes Libraries Life Sciences Marking Open reading frames Proteins Proteomes Proteomics Regulatory sequences Saccharomyces cerevisiae Transcription Yeasts
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