Improving the fermentation performance of Clostridium acetobutylicum ATCC 824 by strengthening the VB1 biosynthesis pathway
Vitamin B1 (VB1) is an essential coenzyme for carbohydrate metabolism and involved in energy generation in most organisms. In this study, we found that insufficient biosynthesis of VB1 in Clostridium acetobutylicum ATCC 824 is a major limiting factor for efficient acetone-butanol-ethanol (ABE) ferme...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2018-09, Vol.102 (18), p.8107-8119 |
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| creator | Liao, Zhengping Suo, Yukai Xue, Chuang Fu, Hongxin Wang, Jufang |
| description | Vitamin B1 (VB1) is an essential coenzyme for carbohydrate metabolism and involved in energy generation in most organisms. In this study, we found that insufficient biosynthesis of VB1 in
Clostridium acetobutylicum
ATCC 824 is a major limiting factor for efficient acetone-butanol-ethanol (ABE) fermentation. In order to improve the fermentation performance of
C. acetobutylicum
ATCC 824, the VB1 biosynthesis pathway was strengthened by overexpressing the
thiC
,
thiG
, and
thiE
genes. The engineered strain 824(thiCGE) showed enhanced VB1 and energy synthesis, resulting in better growth, faster sugar consumption, higher solvents production, and lower acids formation than the wild-type strain in both VB1 free and normal P2 medium (1 mg/L). Compared with the wild-type strain, 824(thiCGE) produced 13.0 ± 0.1% or 12.7 ± 1.2% more butanol in VB1 free P2 medium when glucose or xylose was used as the substrate, respectively. When mixed sugar (glucose:xylose = 2:1) was used as the substrate in VB1 free P2 medium, the xylose consumption rate and butanol titer of 824(thiCGE) were 45.8 ± 1.9% and 20.4 ± 0.3% higher than those of the wild-type strain. All these results demonstrated that this metabolic engineering strategy could provide a new and effective way to improve the cellular performance of solventogenic clostridia. In addition, it may have some potential application value in ABE fermentation using simple medium and/or lignocellulosic biomass. |
| doi_str_mv | 10.1007/s00253-018-9208-x |
| format | Article |
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Clostridium acetobutylicum
ATCC 824 is a major limiting factor for efficient acetone-butanol-ethanol (ABE) fermentation. In order to improve the fermentation performance of
C. acetobutylicum
ATCC 824, the VB1 biosynthesis pathway was strengthened by overexpressing the
thiC
,
thiG
, and
thiE
genes. The engineered strain 824(thiCGE) showed enhanced VB1 and energy synthesis, resulting in better growth, faster sugar consumption, higher solvents production, and lower acids formation than the wild-type strain in both VB1 free and normal P2 medium (1 mg/L). Compared with the wild-type strain, 824(thiCGE) produced 13.0 ± 0.1% or 12.7 ± 1.2% more butanol in VB1 free P2 medium when glucose or xylose was used as the substrate, respectively. When mixed sugar (glucose:xylose = 2:1) was used as the substrate in VB1 free P2 medium, the xylose consumption rate and butanol titer of 824(thiCGE) were 45.8 ± 1.9% and 20.4 ± 0.3% higher than those of the wild-type strain. All these results demonstrated that this metabolic engineering strategy could provide a new and effective way to improve the cellular performance of solventogenic clostridia. In addition, it may have some potential application value in ABE fermentation using simple medium and/or lignocellulosic biomass.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-018-9208-x</identifier><identifier>PMID: 29987383</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acetone ; Bacteria ; Bioenergy and Biofuels ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Butanol ; Carbohydrate metabolism ; Carbohydrates ; Clostridium ; Energy metabolism ; Ethanol ; Fermentation ; Glucose ; Life Sciences ; Lignocellulose ; Metabolic engineering ; Metabolism ; Microbial Genetics and Genomics ; Microbiology ; Solvents ; Substrates ; Sugar ; Vitamin B1 ; Xylose ; Yeast</subject><ispartof>Applied microbiology and biotechnology, 2018-09, Vol.102 (18), p.8107-8119</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>Applied Microbiology and Biotechnology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-b8a0aa9ead79ffbb9c4ee7316468c8fef268601139b1c5148f9c8ecced6f492b3</citedby><cites>FETCH-LOGICAL-c409t-b8a0aa9ead79ffbb9c4ee7316468c8fef268601139b1c5148f9c8ecced6f492b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-018-9208-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-018-9208-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29987383$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liao, Zhengping</creatorcontrib><creatorcontrib>Suo, Yukai</creatorcontrib><creatorcontrib>Xue, Chuang</creatorcontrib><creatorcontrib>Fu, Hongxin</creatorcontrib><creatorcontrib>Wang, Jufang</creatorcontrib><title>Improving the fermentation performance of Clostridium acetobutylicum ATCC 824 by strengthening the VB1 biosynthesis pathway</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Vitamin B1 (VB1) is an essential coenzyme for carbohydrate metabolism and involved in energy generation in most organisms. In this study, we found that insufficient biosynthesis of VB1 in
Clostridium acetobutylicum
ATCC 824 is a major limiting factor for efficient acetone-butanol-ethanol (ABE) fermentation. In order to improve the fermentation performance of
C. acetobutylicum
ATCC 824, the VB1 biosynthesis pathway was strengthened by overexpressing the
thiC
,
thiG
, and
thiE
genes. The engineered strain 824(thiCGE) showed enhanced VB1 and energy synthesis, resulting in better growth, faster sugar consumption, higher solvents production, and lower acids formation than the wild-type strain in both VB1 free and normal P2 medium (1 mg/L). Compared with the wild-type strain, 824(thiCGE) produced 13.0 ± 0.1% or 12.7 ± 1.2% more butanol in VB1 free P2 medium when glucose or xylose was used as the substrate, respectively. When mixed sugar (glucose:xylose = 2:1) was used as the substrate in VB1 free P2 medium, the xylose consumption rate and butanol titer of 824(thiCGE) were 45.8 ± 1.9% and 20.4 ± 0.3% higher than those of the wild-type strain. All these results demonstrated that this metabolic engineering strategy could provide a new and effective way to improve the cellular performance of solventogenic clostridia. In addition, it may have some potential application value in ABE fermentation using simple medium and/or lignocellulosic biomass.</description><subject>Acetone</subject><subject>Bacteria</subject><subject>Bioenergy and Biofuels</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Butanol</subject><subject>Carbohydrate metabolism</subject><subject>Carbohydrates</subject><subject>Clostridium</subject><subject>Energy metabolism</subject><subject>Ethanol</subject><subject>Fermentation</subject><subject>Glucose</subject><subject>Life Sciences</subject><subject>Lignocellulose</subject><subject>Metabolic engineering</subject><subject>Metabolism</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Solvents</subject><subject>Substrates</subject><subject>Sugar</subject><subject>Vitamin 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Yukai</au><au>Xue, Chuang</au><au>Fu, Hongxin</au><au>Wang, Jufang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improving the fermentation performance of Clostridium acetobutylicum ATCC 824 by strengthening the VB1 biosynthesis pathway</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2018-09-01</date><risdate>2018</risdate><volume>102</volume><issue>18</issue><spage>8107</spage><epage>8119</epage><pages>8107-8119</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>Vitamin B1 (VB1) is an essential coenzyme for carbohydrate metabolism and involved in energy generation in most organisms. In this study, we found that insufficient biosynthesis of VB1 in
Clostridium acetobutylicum
ATCC 824 is a major limiting factor for efficient acetone-butanol-ethanol (ABE) fermentation. In order to improve the fermentation performance of
C. acetobutylicum
ATCC 824, the VB1 biosynthesis pathway was strengthened by overexpressing the
thiC
,
thiG
, and
thiE
genes. The engineered strain 824(thiCGE) showed enhanced VB1 and energy synthesis, resulting in better growth, faster sugar consumption, higher solvents production, and lower acids formation than the wild-type strain in both VB1 free and normal P2 medium (1 mg/L). Compared with the wild-type strain, 824(thiCGE) produced 13.0 ± 0.1% or 12.7 ± 1.2% more butanol in VB1 free P2 medium when glucose or xylose was used as the substrate, respectively. When mixed sugar (glucose:xylose = 2:1) was used as the substrate in VB1 free P2 medium, the xylose consumption rate and butanol titer of 824(thiCGE) were 45.8 ± 1.9% and 20.4 ± 0.3% higher than those of the wild-type strain. All these results demonstrated that this metabolic engineering strategy could provide a new and effective way to improve the cellular performance of solventogenic clostridia. In addition, it may have some potential application value in ABE fermentation using simple medium and/or lignocellulosic biomass.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29987383</pmid><doi>10.1007/s00253-018-9208-x</doi><tpages>13</tpages></addata></record> |
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| source | SpringerNature Journals |
| subjects | Acetone Bacteria Bioenergy and Biofuels Biomedical and Life Sciences Biosynthesis Biotechnology Butanol Carbohydrate metabolism Carbohydrates Clostridium Energy metabolism Ethanol Fermentation Glucose Life Sciences Lignocellulose Metabolic engineering Metabolism Microbial Genetics and Genomics Microbiology Solvents Substrates Sugar Vitamin B1 Xylose Yeast |
| title | Improving the fermentation performance of Clostridium acetobutylicum ATCC 824 by strengthening the VB1 biosynthesis pathway |
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