Estrogen-Induced Alterations in amh and dmrt1 Expression Signal for Disruption in Male Sexual Development in the Zebrafish
Dmrt1 and amh are genes involved in vertebrate sex differentiation. In this study, we cloned dmrt1 and amh cDNAs in zebrafish (Danio rerio) and investigated the effects of exposure to 17α-ethinylestradiol (EE2), during early life on their patterns of expression and impact on the subsequent gonadal p...
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| Veröffentlicht in: | Environmental science & technology 2007-09, Vol.41 (17), p.6305-6310 |
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| creator | Schulz, Rüdiger W Bogerd, Jan Male, Rune Ball, Jonathan Fenske, Martina Olsen, Lisbeth C Tyler, Charles R |
| description | Dmrt1 and amh are genes involved in vertebrate sex differentiation. In this study, we cloned dmrt1 and amh cDNAs in zebrafish (Danio rerio) and investigated the effects of exposure to 17α-ethinylestradiol (EE2), during early life on their patterns of expression and impact on the subsequent gonadal phenotype. Expression of both amh and dmrt1 in embryos was detected as early as at 1 day post fertilization (dpf) and enhanced expression of amh from 25 dpf was associated with the period of early gonadal differentiation. Sex-dependent differences in enhanced green fluorescent protein transgene expression driven by the promoter of the germ cell-specific vas gene were exploited to show that at 28dpf and 56dpf both amh and dmrt1 mRNA were overexpressed in males compared with females. Exposure during early life to environmentally relevant concentrations of EE2 had a suppressive effect on the expression of both amh and dmrt1 mRNAs and this was associated with a cessation/retardation in male gonadal sex development. Our findings indicate that estrogen-induced suppression in expression of dmrt1 and amh during early life correlate with subsequent disruptive effects on the sexual phenotype in males. |
| doi_str_mv | 10.1021/es070785+ |
| format | Article |
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In this study, we cloned dmrt1 and amh cDNAs in zebrafish (Danio rerio) and investigated the effects of exposure to 17α-ethinylestradiol (EE2), during early life on their patterns of expression and impact on the subsequent gonadal phenotype. Expression of both amh and dmrt1 in embryos was detected as early as at 1 day post fertilization (dpf) and enhanced expression of amh from 25 dpf was associated with the period of early gonadal differentiation. Sex-dependent differences in enhanced green fluorescent protein transgene expression driven by the promoter of the germ cell-specific vas gene were exploited to show that at 28dpf and 56dpf both amh and dmrt1 mRNA were overexpressed in males compared with females. Exposure during early life to environmentally relevant concentrations of EE2 had a suppressive effect on the expression of both amh and dmrt1 mRNAs and this was associated with a cessation/retardation in male gonadal sex development. Our findings indicate that estrogen-induced suppression in expression of dmrt1 and amh during early life correlate with subsequent disruptive effects on the sexual phenotype in males.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/es070785+</identifier><identifier>PMID: 17937319</identifier><identifier>CODEN: ESTHAG</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animal reproduction ; Animal, plant and microbial ecology ; Animals ; Anti-Mullerian Hormone - genetics ; Applied ecology ; Biological and medical sciences ; Cloning ; Danio rerio ; Ecotoxicology, biological effects of pollution ; Estrogens ; Estrogens - toxicity ; Fish ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Developmental - drug effects ; Gene Expression Regulation, Developmental - physiology ; General aspects ; Genotype & phenotype ; Germ Cells - cytology ; Gonads - drug effects ; Gonads - growth & development ; Male ; Males ; Phenotype ; Proteins ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sexual Development - drug effects ; Sexual Development - physiology ; Time Factors ; Transcription Factors - genetics ; Water Pollutants, Chemical - toxicity ; Zebrafish ; Zebrafish - physiology</subject><ispartof>Environmental science & technology, 2007-09, Vol.41 (17), p.6305-6310</ispartof><rights>2008 INIST-CNRS</rights><rights>Copyright American Chemical Society Sep 1, 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a533t-c09247af00734bd5af3c04e4773319b37633d322df97c974a1a1642df270dc0e3</citedby><cites>FETCH-LOGICAL-a533t-c09247af00734bd5af3c04e4773319b37633d322df97c974a1a1642df270dc0e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/es070785+$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/es070785+$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19050320$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17937319$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schulz, Rüdiger W</creatorcontrib><creatorcontrib>Bogerd, Jan</creatorcontrib><creatorcontrib>Male, Rune</creatorcontrib><creatorcontrib>Ball, Jonathan</creatorcontrib><creatorcontrib>Fenske, Martina</creatorcontrib><creatorcontrib>Olsen, Lisbeth C</creatorcontrib><creatorcontrib>Tyler, Charles R</creatorcontrib><title>Estrogen-Induced Alterations in amh and dmrt1 Expression Signal for Disruption in Male Sexual Development in the Zebrafish</title><title>Environmental science & technology</title><addtitle>Environ. 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Exposure during early life to environmentally relevant concentrations of EE2 had a suppressive effect on the expression of both amh and dmrt1 mRNAs and this was associated with a cessation/retardation in male gonadal sex development. Our findings indicate that estrogen-induced suppression in expression of dmrt1 and amh during early life correlate with subsequent disruptive effects on the sexual phenotype in males.</description><subject>Animal reproduction</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Anti-Mullerian Hormone - genetics</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Cloning</subject><subject>Danio rerio</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Estrogens</subject><subject>Estrogens - toxicity</subject><subject>Fish</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. 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Sci. Technol</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>41</volume><issue>17</issue><spage>6305</spage><epage>6310</epage><pages>6305-6310</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><coden>ESTHAG</coden><abstract>Dmrt1 and amh are genes involved in vertebrate sex differentiation. In this study, we cloned dmrt1 and amh cDNAs in zebrafish (Danio rerio) and investigated the effects of exposure to 17α-ethinylestradiol (EE2), during early life on their patterns of expression and impact on the subsequent gonadal phenotype. Expression of both amh and dmrt1 in embryos was detected as early as at 1 day post fertilization (dpf) and enhanced expression of amh from 25 dpf was associated with the period of early gonadal differentiation. Sex-dependent differences in enhanced green fluorescent protein transgene expression driven by the promoter of the germ cell-specific vas gene were exploited to show that at 28dpf and 56dpf both amh and dmrt1 mRNA were overexpressed in males compared with females. Exposure during early life to environmentally relevant concentrations of EE2 had a suppressive effect on the expression of both amh and dmrt1 mRNAs and this was associated with a cessation/retardation in male gonadal sex development. Our findings indicate that estrogen-induced suppression in expression of dmrt1 and amh during early life correlate with subsequent disruptive effects on the sexual phenotype in males.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>17937319</pmid><doi>10.1021/es070785+</doi><tpages>6</tpages></addata></record> |
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| subjects | Animal reproduction Animal, plant and microbial ecology Animals Anti-Mullerian Hormone - genetics Applied ecology Biological and medical sciences Cloning Danio rerio Ecotoxicology, biological effects of pollution Estrogens Estrogens - toxicity Fish Freshwater Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Developmental - drug effects Gene Expression Regulation, Developmental - physiology General aspects Genotype & phenotype Germ Cells - cytology Gonads - drug effects Gonads - growth & development Male Males Phenotype Proteins RNA, Messenger - genetics RNA, Messenger - metabolism Sexual Development - drug effects Sexual Development - physiology Time Factors Transcription Factors - genetics Water Pollutants, Chemical - toxicity Zebrafish Zebrafish - physiology |
| title | Estrogen-Induced Alterations in amh and dmrt1 Expression Signal for Disruption in Male Sexual Development in the Zebrafish |
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