Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1–4 infections in wild rats from Lithuania
•Rat HEV infects Black (Rattus rattus) and Norway (R. norvegicus) rats from Lithuania.•Partial ORF1-derived sequences of rat HEV show geographically-related clustering.•Prevalence of anti-rat HEV antibodies indicates a high-rate of virus circulation in rats.•The low level of parallel rat HEV RNA and...
Gespeichert in:
| Veröffentlicht in: | Veterinary microbiology 2018-07, Vol.221, p.129-133 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 133 |
|---|---|
| container_issue | |
| container_start_page | 129 |
| container_title | Veterinary microbiology |
| container_volume | 221 |
| creator | Simanavicius, Martynas Juskaite, Karolina Verbickaite, Arune Jasiulionis, Marius Tamosiunas, Paulius Lukas Petraityte-Burneikiene, Rasa Zvirbliene, Aurelija Ulrich, Rainer G. Kucinskaite-Kodze, Indre |
| description | •Rat HEV infects Black (Rattus rattus) and Norway (R. norvegicus) rats from Lithuania.•Partial ORF1-derived sequences of rat HEV show geographically-related clustering.•Prevalence of anti-rat HEV antibodies indicates a high-rate of virus circulation in rats.•The low level of parallel rat HEV RNA and anti-rat HEV antibody detection suggest a non-persistent infection.
Rat hepatitis E virus (HEV) is an orthohepevirus which is related to other HEV found in humans and other mammals. It was first identified in Norway rats (Rattus norvegicus) from Germany in 2010, and later it has been detected in Black rats (Rattus rattus) and Norway rats from USA, China, Indonesia, Vietnam and many European countries. In this study, we describe molecular and serological investigations of Black and Norway rats trapped in Lithuania, Eastern Europe, for infections with rat HEV and human HEV genotypes 1–4. Rat HEV-specific real-time reverse transcription-PCR (RT-qPCR) analysis of rat liver samples revealed the presence of rat HEV in 9 of 109 (8.3%) samples. In contrast, a RT-qPCR specific for HEV genotypes 1–4 did not reveal any positive samples. A nested broad spectrum RT-PCR was used for a confirmation of rat HEV infection with a subsequent sequencing of the amplified rat HEV genome fragment. Phylogenetic analysis revealed a clustering of all newly identified rat HEV sequences with Norway rat-derived rat HEV sequences from Germany within the species Orthohepevirus C. An indirect ELISA using a yeast-expressed truncated rat HEV capsid protein variant revealed 31.2% seropositive samples indicating a high rate of rat HEV circulation in the rat population examined. In conclusion, the current investigation confirms rat HEV infections in Norway and Black rats in Lithuania, Eastern Europe, and the non-persistent nature of HEV infection. |
| doi_str_mv | 10.1016/j.vetmic.2018.06.014 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2067136403</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378113518304097</els_id><sourcerecordid>2097969692</sourcerecordid><originalsourceid>FETCH-LOGICAL-c390t-1c61980f13234ae180d30a377085f6e47dcdb8efc86e731396a77c293e3c54933</originalsourceid><addsrcrecordid>eNp9kc2KFDEQx4Mo7rj6BiIBLx7sttLpycdFkHX9gAEveg6ZdLWTYToZk_TI3jz4Br6hT2KGXj0oSB1SVP3qX6H-hDxm0DJg4sW-PWGZvGs7YKoF0QLr75AVU5I33brv7pIVcKkaxvj6gjzIeQ8AvRZwn1x0WismtFqR76-xoCs-BhpHmmyhOzza4ovP9JqefJrzc7qdCw2xtubJBlrbu_gZg3f_srTWY7k5ImU_v_3oqQ_jop5rSr_6w3DekemY4kQ3vuxmG7x9SO6N9pDx0e17ST69uf549a7ZfHj7_urVpnFcQ2mYE0wrGBnveG-RKRg4WC4lqPUosJeDG7YKR6cESs64FlZK12mO3K17zfklebboHlP8MmMuZvLZ4eFgA8Y5mw6EZFz0cEaf_oXu45xC_V2ltNSiRlepfqFcijknHM0x-cmmG8PAnF0ye7O4ZM4uGRCmulTHntyKz9sJhz9Dv22pwMsFwHqNk8dksvMYHA4-1XuaIfr_b_gFn2umEQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2097969692</pqid></control><display><type>article</type><title>Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1–4 infections in wild rats from Lithuania</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Simanavicius, Martynas ; Juskaite, Karolina ; Verbickaite, Arune ; Jasiulionis, Marius ; Tamosiunas, Paulius Lukas ; Petraityte-Burneikiene, Rasa ; Zvirbliene, Aurelija ; Ulrich, Rainer G. ; Kucinskaite-Kodze, Indre</creator><creatorcontrib>Simanavicius, Martynas ; Juskaite, Karolina ; Verbickaite, Arune ; Jasiulionis, Marius ; Tamosiunas, Paulius Lukas ; Petraityte-Burneikiene, Rasa ; Zvirbliene, Aurelija ; Ulrich, Rainer G. ; Kucinskaite-Kodze, Indre</creatorcontrib><description>•Rat HEV infects Black (Rattus rattus) and Norway (R. norvegicus) rats from Lithuania.•Partial ORF1-derived sequences of rat HEV show geographically-related clustering.•Prevalence of anti-rat HEV antibodies indicates a high-rate of virus circulation in rats.•The low level of parallel rat HEV RNA and anti-rat HEV antibody detection suggest a non-persistent infection.
Rat hepatitis E virus (HEV) is an orthohepevirus which is related to other HEV found in humans and other mammals. It was first identified in Norway rats (Rattus norvegicus) from Germany in 2010, and later it has been detected in Black rats (Rattus rattus) and Norway rats from USA, China, Indonesia, Vietnam and many European countries. In this study, we describe molecular and serological investigations of Black and Norway rats trapped in Lithuania, Eastern Europe, for infections with rat HEV and human HEV genotypes 1–4. Rat HEV-specific real-time reverse transcription-PCR (RT-qPCR) analysis of rat liver samples revealed the presence of rat HEV in 9 of 109 (8.3%) samples. In contrast, a RT-qPCR specific for HEV genotypes 1–4 did not reveal any positive samples. A nested broad spectrum RT-PCR was used for a confirmation of rat HEV infection with a subsequent sequencing of the amplified rat HEV genome fragment. Phylogenetic analysis revealed a clustering of all newly identified rat HEV sequences with Norway rat-derived rat HEV sequences from Germany within the species Orthohepevirus C. An indirect ELISA using a yeast-expressed truncated rat HEV capsid protein variant revealed 31.2% seropositive samples indicating a high rate of rat HEV circulation in the rat population examined. In conclusion, the current investigation confirms rat HEV infections in Norway and Black rats in Lithuania, Eastern Europe, and the non-persistent nature of HEV infection.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2018.06.014</identifier><identifier>PMID: 29981698</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Animals, Wild - virology ; Capsid protein ; Enzyme-linked immunosorbent assay ; Genomes ; Genotype ; Genotype C1 ; Genotypes ; Hepatitis ; Hepatitis Antibodies ; Hepatitis E - epidemiology ; Hepatitis E - veterinary ; Hepatitis E - virology ; Hepatitis E virus (HEV) ; Hepatitis E virus - classification ; Infections ; Lithuania - epidemiology ; Liver ; Mammals ; Phylogeny ; Polymerase chain reaction ; Rat HEV ; Rats ; Rattus norvegicus ; Reverse Transcriptase Polymerase Chain Reaction ; Reverse transcription ; Rodent Diseases - epidemiology ; Rodent Diseases - virology ; Rodents ; Viruses</subject><ispartof>Veterinary microbiology, 2018-07, Vol.221, p.129-133</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Jul 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-1c61980f13234ae180d30a377085f6e47dcdb8efc86e731396a77c293e3c54933</citedby><cites>FETCH-LOGICAL-c390t-1c61980f13234ae180d30a377085f6e47dcdb8efc86e731396a77c293e3c54933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113518304097$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29981698$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simanavicius, Martynas</creatorcontrib><creatorcontrib>Juskaite, Karolina</creatorcontrib><creatorcontrib>Verbickaite, Arune</creatorcontrib><creatorcontrib>Jasiulionis, Marius</creatorcontrib><creatorcontrib>Tamosiunas, Paulius Lukas</creatorcontrib><creatorcontrib>Petraityte-Burneikiene, Rasa</creatorcontrib><creatorcontrib>Zvirbliene, Aurelija</creatorcontrib><creatorcontrib>Ulrich, Rainer G.</creatorcontrib><creatorcontrib>Kucinskaite-Kodze, Indre</creatorcontrib><title>Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1–4 infections in wild rats from Lithuania</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>•Rat HEV infects Black (Rattus rattus) and Norway (R. norvegicus) rats from Lithuania.•Partial ORF1-derived sequences of rat HEV show geographically-related clustering.•Prevalence of anti-rat HEV antibodies indicates a high-rate of virus circulation in rats.•The low level of parallel rat HEV RNA and anti-rat HEV antibody detection suggest a non-persistent infection.
Rat hepatitis E virus (HEV) is an orthohepevirus which is related to other HEV found in humans and other mammals. It was first identified in Norway rats (Rattus norvegicus) from Germany in 2010, and later it has been detected in Black rats (Rattus rattus) and Norway rats from USA, China, Indonesia, Vietnam and many European countries. In this study, we describe molecular and serological investigations of Black and Norway rats trapped in Lithuania, Eastern Europe, for infections with rat HEV and human HEV genotypes 1–4. Rat HEV-specific real-time reverse transcription-PCR (RT-qPCR) analysis of rat liver samples revealed the presence of rat HEV in 9 of 109 (8.3%) samples. In contrast, a RT-qPCR specific for HEV genotypes 1–4 did not reveal any positive samples. A nested broad spectrum RT-PCR was used for a confirmation of rat HEV infection with a subsequent sequencing of the amplified rat HEV genome fragment. Phylogenetic analysis revealed a clustering of all newly identified rat HEV sequences with Norway rat-derived rat HEV sequences from Germany within the species Orthohepevirus C. An indirect ELISA using a yeast-expressed truncated rat HEV capsid protein variant revealed 31.2% seropositive samples indicating a high rate of rat HEV circulation in the rat population examined. In conclusion, the current investigation confirms rat HEV infections in Norway and Black rats in Lithuania, Eastern Europe, and the non-persistent nature of HEV infection.</description><subject>Animals</subject><subject>Animals, Wild - virology</subject><subject>Capsid protein</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Genomes</subject><subject>Genotype</subject><subject>Genotype C1</subject><subject>Genotypes</subject><subject>Hepatitis</subject><subject>Hepatitis Antibodies</subject><subject>Hepatitis E - epidemiology</subject><subject>Hepatitis E - veterinary</subject><subject>Hepatitis E - virology</subject><subject>Hepatitis E virus (HEV)</subject><subject>Hepatitis E virus - classification</subject><subject>Infections</subject><subject>Lithuania - epidemiology</subject><subject>Liver</subject><subject>Mammals</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Rat HEV</subject><subject>Rats</subject><subject>Rattus norvegicus</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Reverse transcription</subject><subject>Rodent Diseases - epidemiology</subject><subject>Rodent Diseases - virology</subject><subject>Rodents</subject><subject>Viruses</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2KFDEQx4Mo7rj6BiIBLx7sttLpycdFkHX9gAEveg6ZdLWTYToZk_TI3jz4Br6hT2KGXj0oSB1SVP3qX6H-hDxm0DJg4sW-PWGZvGs7YKoF0QLr75AVU5I33brv7pIVcKkaxvj6gjzIeQ8AvRZwn1x0WismtFqR76-xoCs-BhpHmmyhOzza4ovP9JqefJrzc7qdCw2xtubJBlrbu_gZg3f_srTWY7k5ImU_v_3oqQ_jop5rSr_6w3DekemY4kQ3vuxmG7x9SO6N9pDx0e17ST69uf549a7ZfHj7_urVpnFcQ2mYE0wrGBnveG-RKRg4WC4lqPUosJeDG7YKR6cESs64FlZK12mO3K17zfklebboHlP8MmMuZvLZ4eFgA8Y5mw6EZFz0cEaf_oXu45xC_V2ltNSiRlepfqFcijknHM0x-cmmG8PAnF0ye7O4ZM4uGRCmulTHntyKz9sJhz9Dv22pwMsFwHqNk8dksvMYHA4-1XuaIfr_b_gFn2umEQ</recordid><startdate>201807</startdate><enddate>201807</enddate><creator>Simanavicius, Martynas</creator><creator>Juskaite, Karolina</creator><creator>Verbickaite, Arune</creator><creator>Jasiulionis, Marius</creator><creator>Tamosiunas, Paulius Lukas</creator><creator>Petraityte-Burneikiene, Rasa</creator><creator>Zvirbliene, Aurelija</creator><creator>Ulrich, Rainer G.</creator><creator>Kucinskaite-Kodze, Indre</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>201807</creationdate><title>Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1–4 infections in wild rats from Lithuania</title><author>Simanavicius, Martynas ; Juskaite, Karolina ; Verbickaite, Arune ; Jasiulionis, Marius ; Tamosiunas, Paulius Lukas ; Petraityte-Burneikiene, Rasa ; Zvirbliene, Aurelija ; Ulrich, Rainer G. ; Kucinskaite-Kodze, Indre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-1c61980f13234ae180d30a377085f6e47dcdb8efc86e731396a77c293e3c54933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Animals, Wild - virology</topic><topic>Capsid protein</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Genomes</topic><topic>Genotype</topic><topic>Genotype C1</topic><topic>Genotypes</topic><topic>Hepatitis</topic><topic>Hepatitis Antibodies</topic><topic>Hepatitis E - epidemiology</topic><topic>Hepatitis E - veterinary</topic><topic>Hepatitis E - virology</topic><topic>Hepatitis E virus (HEV)</topic><topic>Hepatitis E virus - classification</topic><topic>Infections</topic><topic>Lithuania - epidemiology</topic><topic>Liver</topic><topic>Mammals</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Rat HEV</topic><topic>Rats</topic><topic>Rattus norvegicus</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Reverse transcription</topic><topic>Rodent Diseases - epidemiology</topic><topic>Rodent Diseases - virology</topic><topic>Rodents</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simanavicius, Martynas</creatorcontrib><creatorcontrib>Juskaite, Karolina</creatorcontrib><creatorcontrib>Verbickaite, Arune</creatorcontrib><creatorcontrib>Jasiulionis, Marius</creatorcontrib><creatorcontrib>Tamosiunas, Paulius Lukas</creatorcontrib><creatorcontrib>Petraityte-Burneikiene, Rasa</creatorcontrib><creatorcontrib>Zvirbliene, Aurelija</creatorcontrib><creatorcontrib>Ulrich, Rainer G.</creatorcontrib><creatorcontrib>Kucinskaite-Kodze, Indre</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simanavicius, Martynas</au><au>Juskaite, Karolina</au><au>Verbickaite, Arune</au><au>Jasiulionis, Marius</au><au>Tamosiunas, Paulius Lukas</au><au>Petraityte-Burneikiene, Rasa</au><au>Zvirbliene, Aurelija</au><au>Ulrich, Rainer G.</au><au>Kucinskaite-Kodze, Indre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1–4 infections in wild rats from Lithuania</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2018-07</date><risdate>2018</risdate><volume>221</volume><spage>129</spage><epage>133</epage><pages>129-133</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><abstract>•Rat HEV infects Black (Rattus rattus) and Norway (R. norvegicus) rats from Lithuania.•Partial ORF1-derived sequences of rat HEV show geographically-related clustering.•Prevalence of anti-rat HEV antibodies indicates a high-rate of virus circulation in rats.•The low level of parallel rat HEV RNA and anti-rat HEV antibody detection suggest a non-persistent infection.
Rat hepatitis E virus (HEV) is an orthohepevirus which is related to other HEV found in humans and other mammals. It was first identified in Norway rats (Rattus norvegicus) from Germany in 2010, and later it has been detected in Black rats (Rattus rattus) and Norway rats from USA, China, Indonesia, Vietnam and many European countries. In this study, we describe molecular and serological investigations of Black and Norway rats trapped in Lithuania, Eastern Europe, for infections with rat HEV and human HEV genotypes 1–4. Rat HEV-specific real-time reverse transcription-PCR (RT-qPCR) analysis of rat liver samples revealed the presence of rat HEV in 9 of 109 (8.3%) samples. In contrast, a RT-qPCR specific for HEV genotypes 1–4 did not reveal any positive samples. A nested broad spectrum RT-PCR was used for a confirmation of rat HEV infection with a subsequent sequencing of the amplified rat HEV genome fragment. Phylogenetic analysis revealed a clustering of all newly identified rat HEV sequences with Norway rat-derived rat HEV sequences from Germany within the species Orthohepevirus C. An indirect ELISA using a yeast-expressed truncated rat HEV capsid protein variant revealed 31.2% seropositive samples indicating a high rate of rat HEV circulation in the rat population examined. In conclusion, the current investigation confirms rat HEV infections in Norway and Black rats in Lithuania, Eastern Europe, and the non-persistent nature of HEV infection.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>29981698</pmid><doi>10.1016/j.vetmic.2018.06.014</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1135 |
| ispartof | Veterinary microbiology, 2018-07, Vol.221, p.129-133 |
| issn | 0378-1135 1873-2542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2067136403 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Animals, Wild - virology Capsid protein Enzyme-linked immunosorbent assay Genomes Genotype Genotype C1 Genotypes Hepatitis Hepatitis Antibodies Hepatitis E - epidemiology Hepatitis E - veterinary Hepatitis E - virology Hepatitis E virus (HEV) Hepatitis E virus - classification Infections Lithuania - epidemiology Liver Mammals Phylogeny Polymerase chain reaction Rat HEV Rats Rattus norvegicus Reverse Transcriptase Polymerase Chain Reaction Reverse transcription Rodent Diseases - epidemiology Rodent Diseases - virology Rodents Viruses |
| title | Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1–4 infections in wild rats from Lithuania |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T22%3A58%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20rat%20hepatitis%20E%20virus,%20but%20not%20human%20pathogenic%20hepatitis%20E%20virus%20genotype%201%E2%80%934%20infections%20in%20wild%20rats%20from%20Lithuania&rft.jtitle=Veterinary%20microbiology&rft.au=Simanavicius,%20Martynas&rft.date=2018-07&rft.volume=221&rft.spage=129&rft.epage=133&rft.pages=129-133&rft.issn=0378-1135&rft.eissn=1873-2542&rft_id=info:doi/10.1016/j.vetmic.2018.06.014&rft_dat=%3Cproquest_cross%3E2097969692%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2097969692&rft_id=info:pmid/29981698&rft_els_id=S0378113518304097&rfr_iscdi=true |