Insights into the phylogeny of false-branching heterocytous cyanobacteria with the description of Scytonema pachmarhiense sp. nov. isolated from Pachmarhi Biosphere Reserve, India
A false branching cyanobacterium (strain 10A1_PS) was isolated from a freshwater body of the Pachmarhi Biosphere Reserve, India and was characterised using the polyphasic approach. The detailed morphological examination indicated that the strain belonged to the complex genus Scytonema as it exhibite...
Gespeichert in:
Veröffentlicht in: | FEMS microbiology letters 2018-08, Vol.365 (15) |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | 15 |
container_start_page | |
container_title | FEMS microbiology letters |
container_volume | 365 |
creator | Saraf, Aniket Dawda, Himanshu G Suradkar, Archana Batule, Priyanka Behere, Isha Kotulkar, Manasi Kumat, Ankita Singh, Prashant |
description | A false branching cyanobacterium (strain 10A1_PS) was isolated from a freshwater body of the Pachmarhi Biosphere Reserve, India and was characterised using the polyphasic approach. The detailed morphological examination indicated that the strain belonged to the complex genus Scytonema as it exhibited typical false branching character whose frequency increased with age of the culture. As the family Scytonemataceae and the genus Scytonema has been shown to be polyphyletic in many studies, we provide deep insights into the phylogenetic complexities within the family Scytonemataceae based on 16S rRNA gene phylogeny along with complete morphological, molecular and phylogenetic characterisation of the strain. The 16S rRNA gene phylogenetic tree inferred by Bayesian Inference, Neighbor-Joining and Maximum Parsimony methods showed that the strain clustered within the Scytonema sensu stricto clade. The phylogenetic distance and the positioning of the strain clearly indicated it to be different from other Scytonema species. Further analysis using rbcL phylogeny, folded secondary structures of the 16S-23S ITS, p-distance and percentage pairwise similarity matrix clearly distinguished the strain 10A1_PS from the other closely related species. In accordance with the International Code of Nomenclature for Algae, Fungi and Plants, we propose the name of the new species to be Scytonema pachmarhiense. |
doi_str_mv | 10.1093/femsle/fny160 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2067132904</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2399987638</sourcerecordid><originalsourceid>FETCH-LOGICAL-c321t-c020225b63164414aedd8ed234083633269e95d932b402acb79b2d2ebede26703</originalsourceid><addsrcrecordid>eNpdkUtv1DAUhS0Eog9YskWW2LBopn5knHhJKx4jVaIqsI4c-2bsKrGD7SnK7-IP1tNpUcXqXl19Ojr3HITeUbKiRPLzAaY0wvngFyrIC3RM101dCSnal8_2I3SS0i0hpGZEvEZHTMqWcc6P0d-NT25rc8LO54CzBTzbZQxb8AsOAx7UmKDqo_LaOr_FFjLEoJccdgnrRfnQK11OTuE_LtsHAQNJRzdnF_xe4see9jApPCttJxWtA58Ap3mFfbhbYZfCqDIYPMQw4esnCF-4kGYLEfANJIh3cIY33jj1Br16sPX2cZ6iX18-_7z8Vl19_7q5_HRVac5orjRhhLF1LzgVdU1rBca0YBivScsF50xIkGsjOetrwpTuG9kzw6AHA0w0hJ-ijwfdOYbfO0i5m1zSMI7KQ3m_K1k2lDNJ6oJ--A-9Dbvoi7uOcVnSbgRvC1UdKB1DShGGbo6u_Lp0lHT7NrtDm92hzcK_f1Td9ROYf_RTffweVVGgkQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2399987638</pqid></control><display><type>article</type><title>Insights into the phylogeny of false-branching heterocytous cyanobacteria with the description of Scytonema pachmarhiense sp. nov. isolated from Pachmarhi Biosphere Reserve, India</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Saraf, Aniket ; Dawda, Himanshu G ; Suradkar, Archana ; Batule, Priyanka ; Behere, Isha ; Kotulkar, Manasi ; Kumat, Ankita ; Singh, Prashant</creator><creatorcontrib>Saraf, Aniket ; Dawda, Himanshu G ; Suradkar, Archana ; Batule, Priyanka ; Behere, Isha ; Kotulkar, Manasi ; Kumat, Ankita ; Singh, Prashant</creatorcontrib><description>A false branching cyanobacterium (strain 10A1_PS) was isolated from a freshwater body of the Pachmarhi Biosphere Reserve, India and was characterised using the polyphasic approach. The detailed morphological examination indicated that the strain belonged to the complex genus Scytonema as it exhibited typical false branching character whose frequency increased with age of the culture. As the family Scytonemataceae and the genus Scytonema has been shown to be polyphyletic in many studies, we provide deep insights into the phylogenetic complexities within the family Scytonemataceae based on 16S rRNA gene phylogeny along with complete morphological, molecular and phylogenetic characterisation of the strain. The 16S rRNA gene phylogenetic tree inferred by Bayesian Inference, Neighbor-Joining and Maximum Parsimony methods showed that the strain clustered within the Scytonema sensu stricto clade. The phylogenetic distance and the positioning of the strain clearly indicated it to be different from other Scytonema species. Further analysis using rbcL phylogeny, folded secondary structures of the 16S-23S ITS, p-distance and percentage pairwise similarity matrix clearly distinguished the strain 10A1_PS from the other closely related species. In accordance with the International Code of Nomenclature for Algae, Fungi and Plants, we propose the name of the new species to be Scytonema pachmarhiense.</description><identifier>ISSN: 1574-6968</identifier><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1093/femsle/fny160</identifier><identifier>PMID: 29982333</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Algae ; Bayesian analysis ; Biosphere ; Cyanobacteria ; Fungi ; Microbiology ; Morphology ; New species ; Nomenclature ; Phylogenetics ; Phylogeny ; rRNA 16S ; Scytonema ; Scytonemataceae ; Statistical inference</subject><ispartof>FEMS microbiology letters, 2018-08, Vol.365 (15)</ispartof><rights>FEMS 2018.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c321t-c020225b63164414aedd8ed234083633269e95d932b402acb79b2d2ebede26703</citedby><cites>FETCH-LOGICAL-c321t-c020225b63164414aedd8ed234083633269e95d932b402acb79b2d2ebede26703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29982333$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saraf, Aniket</creatorcontrib><creatorcontrib>Dawda, Himanshu G</creatorcontrib><creatorcontrib>Suradkar, Archana</creatorcontrib><creatorcontrib>Batule, Priyanka</creatorcontrib><creatorcontrib>Behere, Isha</creatorcontrib><creatorcontrib>Kotulkar, Manasi</creatorcontrib><creatorcontrib>Kumat, Ankita</creatorcontrib><creatorcontrib>Singh, Prashant</creatorcontrib><title>Insights into the phylogeny of false-branching heterocytous cyanobacteria with the description of Scytonema pachmarhiense sp. nov. isolated from Pachmarhi Biosphere Reserve, India</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>A false branching cyanobacterium (strain 10A1_PS) was isolated from a freshwater body of the Pachmarhi Biosphere Reserve, India and was characterised using the polyphasic approach. The detailed morphological examination indicated that the strain belonged to the complex genus Scytonema as it exhibited typical false branching character whose frequency increased with age of the culture. As the family Scytonemataceae and the genus Scytonema has been shown to be polyphyletic in many studies, we provide deep insights into the phylogenetic complexities within the family Scytonemataceae based on 16S rRNA gene phylogeny along with complete morphological, molecular and phylogenetic characterisation of the strain. The 16S rRNA gene phylogenetic tree inferred by Bayesian Inference, Neighbor-Joining and Maximum Parsimony methods showed that the strain clustered within the Scytonema sensu stricto clade. The phylogenetic distance and the positioning of the strain clearly indicated it to be different from other Scytonema species. Further analysis using rbcL phylogeny, folded secondary structures of the 16S-23S ITS, p-distance and percentage pairwise similarity matrix clearly distinguished the strain 10A1_PS from the other closely related species. In accordance with the International Code of Nomenclature for Algae, Fungi and Plants, we propose the name of the new species to be Scytonema pachmarhiense.</description><subject>Algae</subject><subject>Bayesian analysis</subject><subject>Biosphere</subject><subject>Cyanobacteria</subject><subject>Fungi</subject><subject>Microbiology</subject><subject>Morphology</subject><subject>New species</subject><subject>Nomenclature</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>rRNA 16S</subject><subject>Scytonema</subject><subject>Scytonemataceae</subject><subject>Statistical inference</subject><issn>1574-6968</issn><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkUtv1DAUhS0Eog9YskWW2LBopn5knHhJKx4jVaIqsI4c-2bsKrGD7SnK7-IP1tNpUcXqXl19Ojr3HITeUbKiRPLzAaY0wvngFyrIC3RM101dCSnal8_2I3SS0i0hpGZEvEZHTMqWcc6P0d-NT25rc8LO54CzBTzbZQxb8AsOAx7UmKDqo_LaOr_FFjLEoJccdgnrRfnQK11OTuE_LtsHAQNJRzdnF_xe4see9jApPCttJxWtA58Ap3mFfbhbYZfCqDIYPMQw4esnCF-4kGYLEfANJIh3cIY33jj1Br16sPX2cZ6iX18-_7z8Vl19_7q5_HRVac5orjRhhLF1LzgVdU1rBca0YBivScsF50xIkGsjOetrwpTuG9kzw6AHA0w0hJ-ijwfdOYbfO0i5m1zSMI7KQ3m_K1k2lDNJ6oJ--A-9Dbvoi7uOcVnSbgRvC1UdKB1DShGGbo6u_Lp0lHT7NrtDm92hzcK_f1Td9ROYf_RTffweVVGgkQ</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>Saraf, Aniket</creator><creator>Dawda, Himanshu G</creator><creator>Suradkar, Archana</creator><creator>Batule, Priyanka</creator><creator>Behere, Isha</creator><creator>Kotulkar, Manasi</creator><creator>Kumat, Ankita</creator><creator>Singh, Prashant</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20180801</creationdate><title>Insights into the phylogeny of false-branching heterocytous cyanobacteria with the description of Scytonema pachmarhiense sp. nov. isolated from Pachmarhi Biosphere Reserve, India</title><author>Saraf, Aniket ; Dawda, Himanshu G ; Suradkar, Archana ; Batule, Priyanka ; Behere, Isha ; Kotulkar, Manasi ; Kumat, Ankita ; Singh, Prashant</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c321t-c020225b63164414aedd8ed234083633269e95d932b402acb79b2d2ebede26703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Algae</topic><topic>Bayesian analysis</topic><topic>Biosphere</topic><topic>Cyanobacteria</topic><topic>Fungi</topic><topic>Microbiology</topic><topic>Morphology</topic><topic>New species</topic><topic>Nomenclature</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>rRNA 16S</topic><topic>Scytonema</topic><topic>Scytonemataceae</topic><topic>Statistical inference</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saraf, Aniket</creatorcontrib><creatorcontrib>Dawda, Himanshu G</creatorcontrib><creatorcontrib>Suradkar, Archana</creatorcontrib><creatorcontrib>Batule, Priyanka</creatorcontrib><creatorcontrib>Behere, Isha</creatorcontrib><creatorcontrib>Kotulkar, Manasi</creatorcontrib><creatorcontrib>Kumat, Ankita</creatorcontrib><creatorcontrib>Singh, Prashant</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saraf, Aniket</au><au>Dawda, Himanshu G</au><au>Suradkar, Archana</au><au>Batule, Priyanka</au><au>Behere, Isha</au><au>Kotulkar, Manasi</au><au>Kumat, Ankita</au><au>Singh, Prashant</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insights into the phylogeny of false-branching heterocytous cyanobacteria with the description of Scytonema pachmarhiense sp. nov. isolated from Pachmarhi Biosphere Reserve, India</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2018-08-01</date><risdate>2018</risdate><volume>365</volume><issue>15</issue><issn>1574-6968</issn><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>A false branching cyanobacterium (strain 10A1_PS) was isolated from a freshwater body of the Pachmarhi Biosphere Reserve, India and was characterised using the polyphasic approach. The detailed morphological examination indicated that the strain belonged to the complex genus Scytonema as it exhibited typical false branching character whose frequency increased with age of the culture. As the family Scytonemataceae and the genus Scytonema has been shown to be polyphyletic in many studies, we provide deep insights into the phylogenetic complexities within the family Scytonemataceae based on 16S rRNA gene phylogeny along with complete morphological, molecular and phylogenetic characterisation of the strain. The 16S rRNA gene phylogenetic tree inferred by Bayesian Inference, Neighbor-Joining and Maximum Parsimony methods showed that the strain clustered within the Scytonema sensu stricto clade. The phylogenetic distance and the positioning of the strain clearly indicated it to be different from other Scytonema species. Further analysis using rbcL phylogeny, folded secondary structures of the 16S-23S ITS, p-distance and percentage pairwise similarity matrix clearly distinguished the strain 10A1_PS from the other closely related species. In accordance with the International Code of Nomenclature for Algae, Fungi and Plants, we propose the name of the new species to be Scytonema pachmarhiense.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>29982333</pmid><doi>10.1093/femsle/fny160</doi></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1574-6968 |
ispartof | FEMS microbiology letters, 2018-08, Vol.365 (15) |
issn | 1574-6968 0378-1097 1574-6968 |
language | eng |
recordid | cdi_proquest_miscellaneous_2067132904 |
source | Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
subjects | Algae Bayesian analysis Biosphere Cyanobacteria Fungi Microbiology Morphology New species Nomenclature Phylogenetics Phylogeny rRNA 16S Scytonema Scytonemataceae Statistical inference |
title | Insights into the phylogeny of false-branching heterocytous cyanobacteria with the description of Scytonema pachmarhiense sp. nov. isolated from Pachmarhi Biosphere Reserve, India |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T14%3A31%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Insights%20into%20the%20phylogeny%20of%20false-branching%20heterocytous%20cyanobacteria%20with%20the%20description%20of%20Scytonema%20pachmarhiense%20sp.%20nov.%20isolated%20from%20Pachmarhi%20Biosphere%20Reserve,%20India&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Saraf,%20Aniket&rft.date=2018-08-01&rft.volume=365&rft.issue=15&rft.issn=1574-6968&rft.eissn=1574-6968&rft_id=info:doi/10.1093/femsle/fny160&rft_dat=%3Cproquest_cross%3E2399987638%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2399987638&rft_id=info:pmid/29982333&rfr_iscdi=true |