Identification and characterization of a tospovirus causing chlorotic ringspots on Phalaenopsis orchids
A putative virus-induced disease showing chlorotic ringspots on leaves of Phalaenopsis orchids has been observed in Taiwan for several years. A virus culture, 91-orchid-1, isolated from a Phalaenopsis orchid bearing chlorotic ringspot symptoms was established in Chenopodium quinoa and Nicotiana bent...
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| Veröffentlicht in: | European journal of plant pathology 2008-02, Vol.120 (2), p.199-209 |
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| creator | Zheng, You-Xiu Chen, Ching-Chung Yang, Chia-Jin Yeh, Shyi-Dong Jan, Fuh-Jyh |
| description | A putative virus-induced disease showing chlorotic ringspots on leaves of Phalaenopsis orchids has been observed in Taiwan for several years. A virus culture, 91-orchid-1, isolated from a Phalaenopsis orchid bearing chlorotic ringspot symptoms was established in Chenopodium quinoa and Nicotiana benthamiana, and characterized serologically and biologically. The virus reacted slightly with the antiserum of Watermelon silver mottle virus (WSMoV) but not with those of Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV) and Groundnut ringspot virus (GRSV). Isometric particles measuring about 70-100 nm were observed. Inoculation with isolated virus was conducted to confirm that 91-orchid-1 is the causal agent of chlorotic ringspot disease of Phalaenopsis orchids. To determine the taxonomic relationships of the virus, the conserved region of L RNA and the complete nucleocapsid gene (N gene) were cloned and sequenced. The sequence of conserved region of L RNA shares 83.8, 82.5, 64.4 and 64.9% nucleotide identities and 96.5, 97.7, 67.3 and 67.6% amino acid identities with those of Peanut bud necrosis virus (PBNV), WSMoV, TSWV and INSV, respectively, indicating that 91-orchid-1 is a tospovirus related to WSMoV. The complete nucleotide sequence of the N gene determined from a cDNA clone was found to be 828 nucleotides long encoding 275 amino acids. Sequence analyses of the N gene showed that 91-orchid-1 is an isolate of Capsicum chlorosis virus (CaCV) which has been reported to infect tomato and capsicum plants in Australia and Thailand. 91-orchid-1 is therefore designated as CaCV-Ph. To our knowledge, this is the first formal report of a tospovirus infecting Phalaenopsis orchids. |
| doi_str_mv | 10.1007/s10658-007-9208-7 |
| format | Article |
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A virus culture, 91-orchid-1, isolated from a Phalaenopsis orchid bearing chlorotic ringspot symptoms was established in Chenopodium quinoa and Nicotiana benthamiana, and characterized serologically and biologically. The virus reacted slightly with the antiserum of Watermelon silver mottle virus (WSMoV) but not with those of Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV) and Groundnut ringspot virus (GRSV). Isometric particles measuring about 70-100 nm were observed. Inoculation with isolated virus was conducted to confirm that 91-orchid-1 is the causal agent of chlorotic ringspot disease of Phalaenopsis orchids. To determine the taxonomic relationships of the virus, the conserved region of L RNA and the complete nucleocapsid gene (N gene) were cloned and sequenced. The sequence of conserved region of L RNA shares 83.8, 82.5, 64.4 and 64.9% nucleotide identities and 96.5, 97.7, 67.3 and 67.6% amino acid identities with those of Peanut bud necrosis virus (PBNV), WSMoV, TSWV and INSV, respectively, indicating that 91-orchid-1 is a tospovirus related to WSMoV. The complete nucleotide sequence of the N gene determined from a cDNA clone was found to be 828 nucleotides long encoding 275 amino acids. Sequence analyses of the N gene showed that 91-orchid-1 is an isolate of Capsicum chlorosis virus (CaCV) which has been reported to infect tomato and capsicum plants in Australia and Thailand. 91-orchid-1 is therefore designated as CaCV-Ph. To our knowledge, this is the first formal report of a tospovirus infecting Phalaenopsis orchids.</description><identifier>ISSN: 0929-1873</identifier><identifier>EISSN: 1573-8469</identifier><identifier>DOI: 10.1007/s10658-007-9208-7</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Agriculture ; Amino acids ; Arachis hypogaea ; Biomedical and Life Sciences ; Bunyaviridae ; Capsicum ; Chenopodium quinoa ; Ecology ; Flowers & plants ; Full Research Paper ; Groundnut ringspot virus ; Impatiens necrotic spot virus ; Life Sciences ; Lycopersicon esculentum ; New host record ; Nicotiana benthamiana ; Orchid virus ; Orchidaceae ; Phalaenopsis ; phylogeny ; Plant diseases ; Plant Pathology ; Plant Sciences ; Studies ; Tomato spotted wilt virus ; Tomatoes ; Tospovirus ; Viruses ; Watermelon silver mottle virus</subject><ispartof>European journal of plant pathology, 2008-02, Vol.120 (2), p.199-209</ispartof><rights>KNPV 2007</rights><rights>KNPV 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-b13276143e53c0dddd7d6c5496af34f8f53eb507bef08cf1e0d083eacaacafaf3</citedby><cites>FETCH-LOGICAL-c370t-b13276143e53c0dddd7d6c5496af34f8f53eb507bef08cf1e0d083eacaacafaf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10658-007-9208-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10658-007-9208-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Zheng, You-Xiu</creatorcontrib><creatorcontrib>Chen, Ching-Chung</creatorcontrib><creatorcontrib>Yang, Chia-Jin</creatorcontrib><creatorcontrib>Yeh, Shyi-Dong</creatorcontrib><creatorcontrib>Jan, Fuh-Jyh</creatorcontrib><title>Identification and characterization of a tospovirus causing chlorotic ringspots on Phalaenopsis orchids</title><title>European journal of plant pathology</title><addtitle>Eur J Plant Pathol</addtitle><description>A putative virus-induced disease showing chlorotic ringspots on leaves of Phalaenopsis orchids has been observed in Taiwan for several years. A virus culture, 91-orchid-1, isolated from a Phalaenopsis orchid bearing chlorotic ringspot symptoms was established in Chenopodium quinoa and Nicotiana benthamiana, and characterized serologically and biologically. The virus reacted slightly with the antiserum of Watermelon silver mottle virus (WSMoV) but not with those of Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV) and Groundnut ringspot virus (GRSV). Isometric particles measuring about 70-100 nm were observed. Inoculation with isolated virus was conducted to confirm that 91-orchid-1 is the causal agent of chlorotic ringspot disease of Phalaenopsis orchids. To determine the taxonomic relationships of the virus, the conserved region of L RNA and the complete nucleocapsid gene (N gene) were cloned and sequenced. The sequence of conserved region of L RNA shares 83.8, 82.5, 64.4 and 64.9% nucleotide identities and 96.5, 97.7, 67.3 and 67.6% amino acid identities with those of Peanut bud necrosis virus (PBNV), WSMoV, TSWV and INSV, respectively, indicating that 91-orchid-1 is a tospovirus related to WSMoV. The complete nucleotide sequence of the N gene determined from a cDNA clone was found to be 828 nucleotides long encoding 275 amino acids. Sequence analyses of the N gene showed that 91-orchid-1 is an isolate of Capsicum chlorosis virus (CaCV) which has been reported to infect tomato and capsicum plants in Australia and Thailand. 91-orchid-1 is therefore designated as CaCV-Ph. To our knowledge, this is the first formal report of a tospovirus infecting Phalaenopsis orchids.</description><subject>Agriculture</subject><subject>Amino acids</subject><subject>Arachis hypogaea</subject><subject>Biomedical and Life Sciences</subject><subject>Bunyaviridae</subject><subject>Capsicum</subject><subject>Chenopodium quinoa</subject><subject>Ecology</subject><subject>Flowers & plants</subject><subject>Full Research Paper</subject><subject>Groundnut ringspot virus</subject><subject>Impatiens necrotic spot virus</subject><subject>Life Sciences</subject><subject>Lycopersicon esculentum</subject><subject>New host record</subject><subject>Nicotiana benthamiana</subject><subject>Orchid virus</subject><subject>Orchidaceae</subject><subject>Phalaenopsis</subject><subject>phylogeny</subject><subject>Plant diseases</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Studies</subject><subject>Tomato spotted wilt virus</subject><subject>Tomatoes</subject><subject>Tospovirus</subject><subject>Viruses</subject><subject>Watermelon silver mottle virus</subject><issn>0929-1873</issn><issn>1573-8469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1LxDAQhoMouH78AE8WD96qk6RtkqOIXyAoqOeQTZPdSG1qphX015ulguDBEMhM8rxDeAg5onBGAcQ5UmhqWeayVAxkKbbIgtaCl7Jq1DZZgGKqpFLwXbKH-AoZVIotyOqudf0YfLBmDLEvTN8Wdm2SsaNL4Wu-jL4wxRhxiB8hTVhYM2HoVxnsYopjsEXKbX4escj449p0xvVxwJD7ZNehxQOy402H7vDn3Ccv11fPl7fl_cPN3eXFfWm5gLFcUs5EQyvuam6hzUu0ja0r1RjPKy99zd2yBrF0HqT11EELkjtjTd4-M_vkdJ47pPg-ORz1W0Drus70Lk6oWdakGKsyePIHfI1T6vPfNKOSCqrqJkN0hmyKiMl5PaTwZtKnpqA33vXsXW_KjXctcobNGRw2Vlz6Hfxf6HgOeRO1WaWA-uWJAeUAshIVCP4N2h6SDg</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>Zheng, You-Xiu</creator><creator>Chen, Ching-Chung</creator><creator>Yang, Chia-Jin</creator><creator>Yeh, Shyi-Dong</creator><creator>Jan, Fuh-Jyh</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X2</scope><scope>88A</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7TM</scope></search><sort><creationdate>20080201</creationdate><title>Identification and characterization of a tospovirus causing chlorotic ringspots on Phalaenopsis orchids</title><author>Zheng, You-Xiu ; Chen, Ching-Chung ; Yang, Chia-Jin ; Yeh, Shyi-Dong ; Jan, Fuh-Jyh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-b13276143e53c0dddd7d6c5496af34f8f53eb507bef08cf1e0d083eacaacafaf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Agriculture</topic><topic>Amino acids</topic><topic>Arachis hypogaea</topic><topic>Biomedical and Life Sciences</topic><topic>Bunyaviridae</topic><topic>Capsicum</topic><topic>Chenopodium quinoa</topic><topic>Ecology</topic><topic>Flowers & plants</topic><topic>Full Research Paper</topic><topic>Groundnut ringspot virus</topic><topic>Impatiens necrotic spot virus</topic><topic>Life Sciences</topic><topic>Lycopersicon esculentum</topic><topic>New host record</topic><topic>Nicotiana benthamiana</topic><topic>Orchid virus</topic><topic>Orchidaceae</topic><topic>Phalaenopsis</topic><topic>phylogeny</topic><topic>Plant diseases</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Studies</topic><topic>Tomato spotted wilt virus</topic><topic>Tomatoes</topic><topic>Tospovirus</topic><topic>Viruses</topic><topic>Watermelon silver mottle virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, You-Xiu</creatorcontrib><creatorcontrib>Chen, Ching-Chung</creatorcontrib><creatorcontrib>Yang, Chia-Jin</creatorcontrib><creatorcontrib>Yeh, Shyi-Dong</creatorcontrib><creatorcontrib>Jan, Fuh-Jyh</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Nucleic Acids Abstracts</collection><jtitle>European journal of plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, You-Xiu</au><au>Chen, Ching-Chung</au><au>Yang, Chia-Jin</au><au>Yeh, Shyi-Dong</au><au>Jan, Fuh-Jyh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of a tospovirus causing chlorotic ringspots on Phalaenopsis orchids</atitle><jtitle>European journal of plant pathology</jtitle><stitle>Eur J Plant Pathol</stitle><date>2008-02-01</date><risdate>2008</risdate><volume>120</volume><issue>2</issue><spage>199</spage><epage>209</epage><pages>199-209</pages><issn>0929-1873</issn><eissn>1573-8469</eissn><abstract>A putative virus-induced disease showing chlorotic ringspots on leaves of Phalaenopsis orchids has been observed in Taiwan for several years. A virus culture, 91-orchid-1, isolated from a Phalaenopsis orchid bearing chlorotic ringspot symptoms was established in Chenopodium quinoa and Nicotiana benthamiana, and characterized serologically and biologically. The virus reacted slightly with the antiserum of Watermelon silver mottle virus (WSMoV) but not with those of Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV) and Groundnut ringspot virus (GRSV). Isometric particles measuring about 70-100 nm were observed. Inoculation with isolated virus was conducted to confirm that 91-orchid-1 is the causal agent of chlorotic ringspot disease of Phalaenopsis orchids. To determine the taxonomic relationships of the virus, the conserved region of L RNA and the complete nucleocapsid gene (N gene) were cloned and sequenced. The sequence of conserved region of L RNA shares 83.8, 82.5, 64.4 and 64.9% nucleotide identities and 96.5, 97.7, 67.3 and 67.6% amino acid identities with those of Peanut bud necrosis virus (PBNV), WSMoV, TSWV and INSV, respectively, indicating that 91-orchid-1 is a tospovirus related to WSMoV. The complete nucleotide sequence of the N gene determined from a cDNA clone was found to be 828 nucleotides long encoding 275 amino acids. Sequence analyses of the N gene showed that 91-orchid-1 is an isolate of Capsicum chlorosis virus (CaCV) which has been reported to infect tomato and capsicum plants in Australia and Thailand. 91-orchid-1 is therefore designated as CaCV-Ph. To our knowledge, this is the first formal report of a tospovirus infecting Phalaenopsis orchids.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><doi>10.1007/s10658-007-9208-7</doi><tpages>11</tpages></addata></record> |
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| subjects | Agriculture Amino acids Arachis hypogaea Biomedical and Life Sciences Bunyaviridae Capsicum Chenopodium quinoa Ecology Flowers & plants Full Research Paper Groundnut ringspot virus Impatiens necrotic spot virus Life Sciences Lycopersicon esculentum New host record Nicotiana benthamiana Orchid virus Orchidaceae Phalaenopsis phylogeny Plant diseases Plant Pathology Plant Sciences Studies Tomato spotted wilt virus Tomatoes Tospovirus Viruses Watermelon silver mottle virus |
| title | Identification and characterization of a tospovirus causing chlorotic ringspots on Phalaenopsis orchids |
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