Physiological Improvement to Enhance Escherichia coli Cell- Surface Display via Reducing Extracytoplasmic Stress
Cell physiology was impaired when enhanced yellow fluorescence protein (EYFP) was displayed on the Escherichia coli cell surface, resulting in growth arrest and poor display performance. Coexpression of Skp, a periplasmic chaperone known to interact with several outer membrane proteins for their tra...
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Veröffentlicht in: | Biotechnology progress 2008-01, Vol.24 (2), p.293-301 |
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Sprache: | eng |
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