Molecular discrimination of new isolates of Bifidobacterium animalis subsp. lactis from reference strains and commercial probiotic strains
Fifteen new isolates from pig faeces were identified as Bifidobacterium animalis subsp. lactis by polymerase chain reaction (PCR) using primers specific for this subspecies. Ten of the isolates could be differentiated at strain level by randomly amplified polymorphic DNA (RAPD) analysis and pulsed-f...
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| Veröffentlicht in: | International dairy journal 2007-05, Vol.17 (5), p.565-573 |
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| creator | Mayer, Helmut K. Amtmann, Ernst Philippi, Elisabeth Steinegger, Gudrun Mayrhofer, Sigrid Kneifel, Wolfgang |
| description | Fifteen new isolates from pig faeces were identified as Bifidobacterium animalis subsp. lactis by polymerase chain reaction (PCR) using primers specific for this subspecies. Ten of the isolates could be differentiated at strain level by randomly amplified polymorphic DNA (RAPD) analysis and pulsed-field gel electrophoresis (PFGE). These new strains were different from the type strain and other reference strains of this subspecies, and from all commercial dairy strains with probiotic functionality. Thus, possibly some of the isolates are potential candidates for new probiotic Bifidobacterium strains that can be unambiguously identified by RAPD-PCR and PFGE, which could be of interest for the food industry. In contrast, reference strains and commercial dairy strains revealed great genetic homogeneity, showing almost identical DNA fingerprints using RAPD-PCR and PFGE. Some reference strains and all commercial probiotic strains that had been originally designated as B. animalis or B. lactis have to be assigned to B. animalis subsp. lactis to be correctly labelled. |
| doi_str_mv | 10.1016/j.idairyj.2006.05.008 |
| format | Article |
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Ten of the isolates could be differentiated at strain level by randomly amplified polymorphic DNA (RAPD) analysis and pulsed-field gel electrophoresis (PFGE). These new strains were different from the type strain and other reference strains of this subspecies, and from all commercial dairy strains with probiotic functionality. Thus, possibly some of the isolates are potential candidates for new probiotic Bifidobacterium strains that can be unambiguously identified by RAPD-PCR and PFGE, which could be of interest for the food industry. In contrast, reference strains and commercial dairy strains revealed great genetic homogeneity, showing almost identical DNA fingerprints using RAPD-PCR and PFGE. Some reference strains and all commercial probiotic strains that had been originally designated as B. animalis or B. lactis have to be assigned to B. animalis subsp. lactis to be correctly labelled.</description><identifier>ISSN: 0958-6946</identifier><identifier>EISSN: 1879-0143</identifier><identifier>DOI: 10.1016/j.idairyj.2006.05.008</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Bifidobacterium animalis ; Bifidobacterium animalis subsp. lactis ; Biological and medical sciences ; Food industries ; Fundamental and applied biological sciences. Psychology ; Identification ; intestinal microorganisms ; Milk and cheese industries. Ice creams ; molecular conformation ; nonpathogenic strains ; PCR ; PFGE ; probiotic bacteria ; Probiotics ; RAPD-PCR ; strain differences</subject><ispartof>International dairy journal, 2007-05, Vol.17 (5), p.565-573</ispartof><rights>2006 Elsevier Ltd</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c309t-d91568d4952d63dcdb25d8b70d0ec8fc1997f5b4d0d31b8e410d203843c6800e3</citedby><cites>FETCH-LOGICAL-c309t-d91568d4952d63dcdb25d8b70d0ec8fc1997f5b4d0d31b8e410d203843c6800e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.idairyj.2006.05.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27928,27929,45999</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18486099$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Mayer, Helmut K.</creatorcontrib><creatorcontrib>Amtmann, Ernst</creatorcontrib><creatorcontrib>Philippi, Elisabeth</creatorcontrib><creatorcontrib>Steinegger, Gudrun</creatorcontrib><creatorcontrib>Mayrhofer, Sigrid</creatorcontrib><creatorcontrib>Kneifel, Wolfgang</creatorcontrib><title>Molecular discrimination of new isolates of Bifidobacterium animalis subsp. lactis from reference strains and commercial probiotic strains</title><title>International dairy journal</title><description>Fifteen new isolates from pig faeces were identified as Bifidobacterium animalis subsp. lactis by polymerase chain reaction (PCR) using primers specific for this subspecies. Ten of the isolates could be differentiated at strain level by randomly amplified polymorphic DNA (RAPD) analysis and pulsed-field gel electrophoresis (PFGE). These new strains were different from the type strain and other reference strains of this subspecies, and from all commercial dairy strains with probiotic functionality. Thus, possibly some of the isolates are potential candidates for new probiotic Bifidobacterium strains that can be unambiguously identified by RAPD-PCR and PFGE, which could be of interest for the food industry. In contrast, reference strains and commercial dairy strains revealed great genetic homogeneity, showing almost identical DNA fingerprints using RAPD-PCR and PFGE. Some reference strains and all commercial probiotic strains that had been originally designated as B. animalis or B. lactis have to be assigned to B. animalis subsp. lactis to be correctly labelled.</description><subject>Bifidobacterium animalis</subject><subject>Bifidobacterium animalis subsp. lactis</subject><subject>Biological and medical sciences</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Identification</subject><subject>intestinal microorganisms</subject><subject>Milk and cheese industries. Ice creams</subject><subject>molecular conformation</subject><subject>nonpathogenic strains</subject><subject>PCR</subject><subject>PFGE</subject><subject>probiotic bacteria</subject><subject>Probiotics</subject><subject>RAPD-PCR</subject><subject>strain differences</subject><issn>0958-6946</issn><issn>1879-0143</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkM1u1DAQgCMEEkvhERC-wC1hHMeJfUJQ8Se16qH0bDn2BM3KiRc7adVX4Knxahdx5DQazTd_X1W95tBw4P37fUPeUnrcNy1A34BsANSTasfVoGvgnXha7UBLVfe6659XL3LeA_ABhN5Vv69jQLcFm5in7BLNtNiV4sLixBZ8YJRjsCvmY_6JJvJxtG7FRNvM7EKzDZRZ3sZ8aFgolZJNKc4s4YQJF4csr8nSkgvtmYvzjMmRDeyQ4khxJfcXeFk9m2zI-OocL6q7L59_XH6rr26-fr_8eFU7AXqtveayV77TsvW98M6PrfRqHMADOjU5rvUwybHz4AUfFXYcfAtCdcL1CgDFRfXuNLdc8GvDvJq5fI4h2AXjlk0LvWy1agsoT6BLMefykDkUPzY9Gg7maN7szdm8OZo3IE0xX_renhfY7GyYkl0c5X_NqlM9aF24NydustHYn6kwd7ctcAEwSKGFLMSHE4HFxz1hMtnRUaqnhG41PtJ_bvkDh1mpEw</recordid><startdate>200705</startdate><enddate>200705</enddate><creator>Mayer, Helmut K.</creator><creator>Amtmann, Ernst</creator><creator>Philippi, Elisabeth</creator><creator>Steinegger, Gudrun</creator><creator>Mayrhofer, Sigrid</creator><creator>Kneifel, Wolfgang</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>200705</creationdate><title>Molecular discrimination of new isolates of Bifidobacterium animalis subsp. lactis from reference strains and commercial probiotic strains</title><author>Mayer, Helmut K. ; Amtmann, Ernst ; Philippi, Elisabeth ; Steinegger, Gudrun ; Mayrhofer, Sigrid ; Kneifel, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c309t-d91568d4952d63dcdb25d8b70d0ec8fc1997f5b4d0d31b8e410d203843c6800e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Bifidobacterium animalis</topic><topic>Bifidobacterium animalis subsp. lactis</topic><topic>Biological and medical sciences</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. 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Ice creams</topic><topic>molecular conformation</topic><topic>nonpathogenic strains</topic><topic>PCR</topic><topic>PFGE</topic><topic>probiotic bacteria</topic><topic>Probiotics</topic><topic>RAPD-PCR</topic><topic>strain differences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mayer, Helmut K.</creatorcontrib><creatorcontrib>Amtmann, Ernst</creatorcontrib><creatorcontrib>Philippi, Elisabeth</creatorcontrib><creatorcontrib>Steinegger, Gudrun</creatorcontrib><creatorcontrib>Mayrhofer, Sigrid</creatorcontrib><creatorcontrib>Kneifel, Wolfgang</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International dairy journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mayer, Helmut K.</au><au>Amtmann, Ernst</au><au>Philippi, Elisabeth</au><au>Steinegger, Gudrun</au><au>Mayrhofer, Sigrid</au><au>Kneifel, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular discrimination of new isolates of Bifidobacterium animalis subsp. lactis from reference strains and commercial probiotic strains</atitle><jtitle>International dairy journal</jtitle><date>2007-05</date><risdate>2007</risdate><volume>17</volume><issue>5</issue><spage>565</spage><epage>573</epage><pages>565-573</pages><issn>0958-6946</issn><eissn>1879-0143</eissn><abstract>Fifteen new isolates from pig faeces were identified as Bifidobacterium animalis subsp. lactis by polymerase chain reaction (PCR) using primers specific for this subspecies. Ten of the isolates could be differentiated at strain level by randomly amplified polymorphic DNA (RAPD) analysis and pulsed-field gel electrophoresis (PFGE). These new strains were different from the type strain and other reference strains of this subspecies, and from all commercial dairy strains with probiotic functionality. Thus, possibly some of the isolates are potential candidates for new probiotic Bifidobacterium strains that can be unambiguously identified by RAPD-PCR and PFGE, which could be of interest for the food industry. In contrast, reference strains and commercial dairy strains revealed great genetic homogeneity, showing almost identical DNA fingerprints using RAPD-PCR and PFGE. 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| ispartof | International dairy journal, 2007-05, Vol.17 (5), p.565-573 |
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| subjects | Bifidobacterium animalis Bifidobacterium animalis subsp. lactis Biological and medical sciences Food industries Fundamental and applied biological sciences. Psychology Identification intestinal microorganisms Milk and cheese industries. Ice creams molecular conformation nonpathogenic strains PCR PFGE probiotic bacteria Probiotics RAPD-PCR strain differences |
| title | Molecular discrimination of new isolates of Bifidobacterium animalis subsp. lactis from reference strains and commercial probiotic strains |
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