Establishment and characteristics of two syngeneic human osteosarcoma cell lines from primary tumor and skip metastases
Aim： To characterize and compare the different biological behaviors of 2 novel human osteosarcoma cell lines, Zos and Zos-M, established respectively from the primary tumor and the skip metastasis of an osteosarcoma patient. Methods： In vitro studies included morphological observations, karyotype an...
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| Veröffentlicht in: | Acta pharmacologica Sinica 2008-03, Vol.29 (3), p.325-332 |
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| creator | Zou, Chang-ye Wang, Jin Shen, Jing-nan Huang, Gang Jin, Song Yin, Jun-qiang Guo, Qian-chen Li, Hao-miao Luo, Lan Zhang, Meng Zhang, Long-juan |
| description | Aim： To characterize and compare the different biological behaviors of 2 novel human osteosarcoma cell lines, Zos and Zos-M, established respectively from the primary tumor and the skip metastasis of an osteosarcoma patient. Methods： In vitro studies included morphological observations, karyotype analysis, 3-（4,5- dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide cell proliferation assay, and cell sensitivity to chemotherapeutic drugs. Subcutaneous and intravenous inoculations into nude mice were carried out to study the tumorigenicity and the metastatic potential. RT-PCR was performed to assess the expression of the osteoblastic markers and some metastasis-related genes. Results： Both cell lines remained stable for more than 100 passages in vitro without interruption. The RT- PCR examination indicated that they retained the molecular characteristics of an osteoblastic lineage. The karyotype analysis displayed aneuploidy and various structural abnormalities. Both cell lines are tumorigenic; Zos-M differs from Zos by the former＇s ability to develop lung metastasis after intravenous injection. The comparison of the expression patterns of some metastasis-related genes revealed that the decreased expression of cadherin-11 in Zos-M may correlate with a high potential of metastases. Moreover, both cell lines are less sensitive to the current chemotherapy protocols. Conclusion： The establishment of osteosarcoma cell lines, Zos and Zos-M, and related animal models provide a useful resource for studying the aggressive behavior of osteosarcoma and will be helpful for screening effective treatment strategies. |
| doi_str_mv | 10.1111/j.1745-7254.2008.00756.x |
| format | Article |
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Methods： In vitro studies included morphological observations, karyotype analysis, 3-（4,5- dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide cell proliferation assay, and cell sensitivity to chemotherapeutic drugs. Subcutaneous and intravenous inoculations into nude mice were carried out to study the tumorigenicity and the metastatic potential. RT-PCR was performed to assess the expression of the osteoblastic markers and some metastasis-related genes. Results： Both cell lines remained stable for more than 100 passages in vitro without interruption. The RT- PCR examination indicated that they retained the molecular characteristics of an osteoblastic lineage. The karyotype analysis displayed aneuploidy and various structural abnormalities. Both cell lines are tumorigenic; Zos-M differs from Zos by the former＇s ability to develop lung metastasis after intravenous injection. The comparison of the expression patterns of some metastasis-related genes revealed that the decreased expression of cadherin-11 in Zos-M may correlate with a high potential of metastases. Moreover, both cell lines are less sensitive to the current chemotherapy protocols. Conclusion： The establishment of osteosarcoma cell lines, Zos and Zos-M, and related animal models provide a useful resource for studying the aggressive behavior of osteosarcoma and will be helpful for screening effective treatment strategies.</description><identifier>ISSN: 1671-4083</identifier><identifier>EISSN: 1745-7254</identifier><identifier>DOI: 10.1111/j.1745-7254.2008.00756.x</identifier><identifier>PMID: 18298897</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Adolescent ; Animals ; Antimetabolites, Antineoplastic - pharmacology ; Biomarkers, Tumor - metabolism ; Biomedical and Life Sciences ; Biomedicine ; Cell Line, Tumor ; Cell Lineage ; Cell Proliferation - drug effects ; Collagen - metabolism ; Drug Combinations ; Extracellular Matrix - metabolism ; Formazans - metabolism ; Humans ; Immunology ; Inhibitory Concentration 50 ; Internal Medicine ; Karyotyping ; Laminin - metabolism ; Male ; Medical Microbiology ; Methotrexate - pharmacology ; Mice ; Mice, Nude ; Neoplasm Metastasis - pathology ; original-article ; Osteosarcoma - genetics ; Osteosarcoma - pathology ; Osteosarcoma - secondary ; Osteosarcoma - ultrastructure ; Pharmacology/Toxicology ; Proteoglycans - metabolism ; Tetrazolium Salts - metabolism ; Vaccine ; Xenograft Model Antitumor Assays ; 人类 ; 基因 ; 细胞 ; 肿瘤 ; 药物 ; 骨肉瘤</subject><ispartof>Acta pharmacologica Sinica, 2008-03, Vol.29 (3), p.325-332</ispartof><rights>CPS and SIMM 2008</rights><rights>Copyright Nature Publishing Group Mar 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-569f5e6df6314f4a03d3899ff3c9950a7b8f4381f78fd1ca9a2d8c5befd9df763</citedby><cites>FETCH-LOGICAL-c498t-569f5e6df6314f4a03d3899ff3c9950a7b8f4381f78fd1ca9a2d8c5befd9df763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/95561A/95561A.jpg</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18298897$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zou, Chang-ye</creatorcontrib><creatorcontrib>Wang, Jin</creatorcontrib><creatorcontrib>Shen, Jing-nan</creatorcontrib><creatorcontrib>Huang, Gang</creatorcontrib><creatorcontrib>Jin, Song</creatorcontrib><creatorcontrib>Yin, Jun-qiang</creatorcontrib><creatorcontrib>Guo, Qian-chen</creatorcontrib><creatorcontrib>Li, Hao-miao</creatorcontrib><creatorcontrib>Luo, Lan</creatorcontrib><creatorcontrib>Zhang, Meng</creatorcontrib><creatorcontrib>Zhang, Long-juan</creatorcontrib><title>Establishment and characteristics of two syngeneic human osteosarcoma cell lines from primary tumor and skip metastases</title><title>Acta pharmacologica Sinica</title><addtitle>Acta Pharmacol Sin</addtitle><addtitle>Acta Pharmacologica Sinica</addtitle><description>Aim： To characterize and compare the different biological behaviors of 2 novel human osteosarcoma cell lines, Zos and Zos-M, established respectively from the primary tumor and the skip metastasis of an osteosarcoma patient. Methods： In vitro studies included morphological observations, karyotype analysis, 3-（4,5- dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide cell proliferation assay, and cell sensitivity to chemotherapeutic drugs. Subcutaneous and intravenous inoculations into nude mice were carried out to study the tumorigenicity and the metastatic potential. RT-PCR was performed to assess the expression of the osteoblastic markers and some metastasis-related genes. Results： Both cell lines remained stable for more than 100 passages in vitro without interruption. The RT- PCR examination indicated that they retained the molecular characteristics of an osteoblastic lineage. The karyotype analysis displayed aneuploidy and various structural abnormalities. Both cell lines are tumorigenic; Zos-M differs from Zos by the former＇s ability to develop lung metastasis after intravenous injection. The comparison of the expression patterns of some metastasis-related genes revealed that the decreased expression of cadherin-11 in Zos-M may correlate with a high potential of metastases. Moreover, both cell lines are less sensitive to the current chemotherapy protocols. Conclusion： The establishment of osteosarcoma cell lines, Zos and Zos-M, and related animal models provide a useful resource for studying the aggressive behavior of osteosarcoma and will be helpful for screening effective treatment strategies.</description><subject>Adolescent</subject><subject>Animals</subject><subject>Antimetabolites, Antineoplastic - pharmacology</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Line, Tumor</subject><subject>Cell Lineage</subject><subject>Cell Proliferation - drug effects</subject><subject>Collagen - metabolism</subject><subject>Drug Combinations</subject><subject>Extracellular Matrix - metabolism</subject><subject>Formazans - metabolism</subject><subject>Humans</subject><subject>Immunology</subject><subject>Inhibitory Concentration 50</subject><subject>Internal Medicine</subject><subject>Karyotyping</subject><subject>Laminin - metabolism</subject><subject>Male</subject><subject>Medical Microbiology</subject><subject>Methotrexate - pharmacology</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Neoplasm Metastasis - pathology</subject><subject>original-article</subject><subject>Osteosarcoma - genetics</subject><subject>Osteosarcoma - pathology</subject><subject>Osteosarcoma - secondary</subject><subject>Osteosarcoma - ultrastructure</subject><subject>Pharmacology/Toxicology</subject><subject>Proteoglycans - metabolism</subject><subject>Tetrazolium Salts - metabolism</subject><subject>Vaccine</subject><subject>Xenograft Model Antitumor Assays</subject><subject>人类</subject><subject>基因</subject><subject>细胞</subject><subject>肿瘤</subject><subject>药物</subject><subject>骨肉瘤</subject><issn>1671-4083</issn><issn>1745-7254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNUcFu3CAURFWrJtn2FyrUQ292wdgGjlWUtpEi9dKeEcaQ9cbAhoeV5O-Ds1Yj9VQuIL2ZecMMQpiSmpbz9VBT3nYVb7q2bggRNSG86-vHN-j87-BtefecVi0R7AxdABwIYQ2j8j06o6KRQkh-jh6uIOthnmDvbchYhxGbvU7aZJsmyJMBHB3ODxHDU7i1wU4G7xevA46QbQSdTPQaGzvPeJ6CBexS9PiYJq_TE86Lj-lFFe6mI_Y267IPLHxA75yewX7c7h368_3q9-XP6ubXj-vLbzeVaaXIVddL19l-dD2jrWs1YSMTUjrHjJQd0XwQrmWCOi7cSI2WuhmF6QbrRjk63rMd-nLSPaZ4v1jIyk-wutXBxgVUQ_pW0pLMDn3-B3iISwrFm2ooIyVQIgtInEAmRYBkndo-qihRazPqoNYC1FqAWptRL82ox0L9tOkvg7fjK3GrogDkCQBlVKJOrwb-Q3wzb_Yx3N4Xuhq0uXPTbFXTC0o7ztgzc02qdA</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Zou, Chang-ye</creator><creator>Wang, Jin</creator><creator>Shen, Jing-nan</creator><creator>Huang, Gang</creator><creator>Jin, Song</creator><creator>Yin, Jun-qiang</creator><creator>Guo, Qian-chen</creator><creator>Li, Hao-miao</creator><creator>Luo, Lan</creator><creator>Zhang, Meng</creator><creator>Zhang, Long-juan</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20080301</creationdate><title>Establishment and characteristics of two syngeneic human osteosarcoma cell lines from primary tumor and skip metastases</title><author>Zou, Chang-ye ; Wang, Jin ; Shen, Jing-nan ; Huang, Gang ; Jin, Song ; Yin, Jun-qiang ; Guo, Qian-chen ; Li, Hao-miao ; Luo, Lan ; Zhang, Meng ; Zhang, Long-juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-569f5e6df6314f4a03d3899ff3c9950a7b8f4381f78fd1ca9a2d8c5befd9df763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adolescent</topic><topic>Animals</topic><topic>Antimetabolites, Antineoplastic - pharmacology</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Line, Tumor</topic><topic>Cell Lineage</topic><topic>Cell Proliferation - drug effects</topic><topic>Collagen - metabolism</topic><topic>Drug Combinations</topic><topic>Extracellular Matrix - metabolism</topic><topic>Formazans - metabolism</topic><topic>Humans</topic><topic>Immunology</topic><topic>Inhibitory Concentration 50</topic><topic>Internal Medicine</topic><topic>Karyotyping</topic><topic>Laminin - metabolism</topic><topic>Male</topic><topic>Medical Microbiology</topic><topic>Methotrexate - pharmacology</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Neoplasm Metastasis - pathology</topic><topic>original-article</topic><topic>Osteosarcoma - genetics</topic><topic>Osteosarcoma - pathology</topic><topic>Osteosarcoma - secondary</topic><topic>Osteosarcoma - ultrastructure</topic><topic>Pharmacology/Toxicology</topic><topic>Proteoglycans - metabolism</topic><topic>Tetrazolium Salts - metabolism</topic><topic>Vaccine</topic><topic>Xenograft Model Antitumor Assays</topic><topic>人类</topic><topic>基因</topic><topic>细胞</topic><topic>肿瘤</topic><topic>药物</topic><topic>骨肉瘤</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zou, Chang-ye</creatorcontrib><creatorcontrib>Wang, Jin</creatorcontrib><creatorcontrib>Shen, Jing-nan</creatorcontrib><creatorcontrib>Huang, Gang</creatorcontrib><creatorcontrib>Jin, Song</creatorcontrib><creatorcontrib>Yin, Jun-qiang</creatorcontrib><creatorcontrib>Guo, Qian-chen</creatorcontrib><creatorcontrib>Li, Hao-miao</creatorcontrib><creatorcontrib>Luo, Lan</creatorcontrib><creatorcontrib>Zhang, Meng</creatorcontrib><creatorcontrib>Zhang, Long-juan</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Acta pharmacologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zou, Chang-ye</au><au>Wang, Jin</au><au>Shen, Jing-nan</au><au>Huang, Gang</au><au>Jin, Song</au><au>Yin, Jun-qiang</au><au>Guo, Qian-chen</au><au>Li, Hao-miao</au><au>Luo, Lan</au><au>Zhang, Meng</au><au>Zhang, Long-juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment and characteristics of two syngeneic human osteosarcoma cell lines from primary tumor and skip metastases</atitle><jtitle>Acta pharmacologica Sinica</jtitle><stitle>Acta Pharmacol Sin</stitle><addtitle>Acta Pharmacologica Sinica</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>29</volume><issue>3</issue><spage>325</spage><epage>332</epage><pages>325-332</pages><issn>1671-4083</issn><eissn>1745-7254</eissn><abstract>Aim： To characterize and compare the different biological behaviors of 2 novel human osteosarcoma cell lines, Zos and Zos-M, established respectively from the primary tumor and the skip metastasis of an osteosarcoma patient. Methods： In vitro studies included morphological observations, karyotype analysis, 3-（4,5- dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide cell proliferation assay, and cell sensitivity to chemotherapeutic drugs. Subcutaneous and intravenous inoculations into nude mice were carried out to study the tumorigenicity and the metastatic potential. RT-PCR was performed to assess the expression of the osteoblastic markers and some metastasis-related genes. Results： Both cell lines remained stable for more than 100 passages in vitro without interruption. The RT- PCR examination indicated that they retained the molecular characteristics of an osteoblastic lineage. The karyotype analysis displayed aneuploidy and various structural abnormalities. Both cell lines are tumorigenic; Zos-M differs from Zos by the former＇s ability to develop lung metastasis after intravenous injection. The comparison of the expression patterns of some metastasis-related genes revealed that the decreased expression of cadherin-11 in Zos-M may correlate with a high potential of metastases. Moreover, both cell lines are less sensitive to the current chemotherapy protocols. Conclusion： The establishment of osteosarcoma cell lines, Zos and Zos-M, and related animal models provide a useful resource for studying the aggressive behavior of osteosarcoma and will be helpful for screening effective treatment strategies.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>18298897</pmid><doi>10.1111/j.1745-7254.2008.00756.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Animals Antimetabolites, Antineoplastic - pharmacology Biomarkers, Tumor - metabolism Biomedical and Life Sciences Biomedicine Cell Line, Tumor Cell Lineage Cell Proliferation - drug effects Collagen - metabolism Drug Combinations Extracellular Matrix - metabolism Formazans - metabolism Humans Immunology Inhibitory Concentration 50 Internal Medicine Karyotyping Laminin - metabolism Male Medical Microbiology Methotrexate - pharmacology Mice Mice, Nude Neoplasm Metastasis - pathology original-article Osteosarcoma - genetics Osteosarcoma - pathology Osteosarcoma - secondary Osteosarcoma - ultrastructure Pharmacology/Toxicology Proteoglycans - metabolism Tetrazolium Salts - metabolism Vaccine Xenograft Model Antitumor Assays 人类 基因 细胞 肿瘤 药物 骨肉瘤 |
| title | Establishment and characteristics of two syngeneic human osteosarcoma cell lines from primary tumor and skip metastases |
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