Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum)
Pea (Pisum sativum) is one of relatively few genetically amenable plant species with compound leaves. Pea leaves have a variety of specialized organs: leaflets, tendrils, pulvini and stipules, which enable the identification of mutations that transform or affect distinct parts of the leaf. Character...
Gespeichert in:
| Veröffentlicht in: | The New phytologist 2018-10, Vol.220 (1), p.288-299 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 299 |
|---|---|
| container_issue | 1 |
| container_start_page | 288 |
| container_title | The New phytologist |
| container_volume | 220 |
| creator | Moreau, Carol Hofer, Julie M. I. Eléouët, Morgane Sinjushin, Andrey Ambrose, Mike Skøt, Kirsten Blackmore, Tina Swain, Martin Hegarty, Matthew Balanzà, Vicente Ferrándiz, Cristina Ellis, T. H. Noel |
| description | Pea (Pisum sativum) is one of relatively few genetically amenable plant species with compound leaves. Pea leaves have a variety of specialized organs: leaflets, tendrils, pulvini and stipules, which enable the identification of mutations that transform or affect distinct parts of the leaf. Characterization of these mutations offers insights into the development and evolution of novel leaf traits. The previously characterized morphological gene Cochleata, conferring stipule identity, was known to interact with Stipules reduced (St), which conditions stipule size in pea, but the St gene remained unknown.
Here we analysed Fast Neutron irradiated pea mutants by restriction site associated DNA sequencing.
We identified St as a gene encoding a C2H2 zinc finger transcription factor that is regulated by Cochleata. St regulates both cell division and cell expansion in the stipule.
Our approach shows how systematic genome-wide screens can be used successfully for the analysis of traits in species for which whole genome sequences are not available. |
| doi_str_mv | 10.1111/nph.15286 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2064766830</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>90024552</jstor_id><sourcerecordid>90024552</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4106-1f82b5692d899589916bc32536d2dcbf7d9493351da946e6f3067ed81534f62d3</originalsourceid><addsrcrecordid>eNp1kV1rFTEQhoMo9li98AcoAW9a6Lb5nE0upagtlFpRwbtlz2bS5rC7WZNd5fx70562F0IHhrl55mF4h5C3nB3zUifjdHPMtTDwjKy4AlsZLuvnZMWYMBUo-LVHXuW8YYxZDeIl2RPW1kqBWZFv5w7HOfjQtXOII42efp_DtPSYaUK3dOiOaEt7bD0dYppuYh-vt_QaR6RhpBO29OAq5GWguQj-LMPha_LCt33GN_dzn_z8_OnH6Vl18fXL-enHi6pTnEHFvRFrDVY4Y60uzWHdSaElOOG6ta-dVVZKzV1rFSB4yaBGZ7iWyoNwcp8c7LxTir8XzHMzhNxh37cjxiU3goGqAYxkBf3wH7qJSxrLdYWymtcG7C11uKO6FHNO6JsphaFN24az5jbnpuTc3OVc2Pf3xmU9oHskH4ItwMkO-Bt63D5tai6vzh6U73YbmzzH9LhhyxOV1kL-AxtCjmI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2095178690</pqid></control><display><type>article</type><title>Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum)</title><source>Wiley-Blackwell Journals</source><source>Wiley-Blackwell Backfiles (Open access)</source><source>MEDLINE</source><source>JSTOR</source><source>EZB Electronic Journals Library</source><creator>Moreau, Carol ; Hofer, Julie M. I. ; Eléouët, Morgane ; Sinjushin, Andrey ; Ambrose, Mike ; Skøt, Kirsten ; Blackmore, Tina ; Swain, Martin ; Hegarty, Matthew ; Balanzà, Vicente ; Ferrándiz, Cristina ; Ellis, T. H. Noel</creator><creatorcontrib>Moreau, Carol ; Hofer, Julie M. I. ; Eléouët, Morgane ; Sinjushin, Andrey ; Ambrose, Mike ; Skøt, Kirsten ; Blackmore, Tina ; Swain, Martin ; Hegarty, Matthew ; Balanzà, Vicente ; Ferrándiz, Cristina ; Ellis, T. H. Noel</creatorcontrib><description>Pea (Pisum sativum) is one of relatively few genetically amenable plant species with compound leaves. Pea leaves have a variety of specialized organs: leaflets, tendrils, pulvini and stipules, which enable the identification of mutations that transform or affect distinct parts of the leaf. Characterization of these mutations offers insights into the development and evolution of novel leaf traits. The previously characterized morphological gene Cochleata, conferring stipule identity, was known to interact with Stipules reduced (St), which conditions stipule size in pea, but the St gene remained unknown.
Here we analysed Fast Neutron irradiated pea mutants by restriction site associated DNA sequencing.
We identified St as a gene encoding a C2H2 zinc finger transcription factor that is regulated by Cochleata. St regulates both cell division and cell expansion in the stipule.
Our approach shows how systematic genome-wide screens can be used successfully for the analysis of traits in species for which whole genome sequences are not available.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.15286</identifier><identifier>PMID: 29974468</identifier><language>eng</language><publisher>England: New Phytologist Trust</publisher><subject>Body organs ; C2H2 zinc finger ; Cell division ; Deoxyribonucleic acid ; DNA ; DNA sequences ; DNA sequencing ; Fast Neutron ; Fast neutrons ; Gene Expression Regulation, Plant ; Genes, Plant ; Genetic Association Studies ; Genomes ; Identification ; leaf ; Leaves ; Medicago - genetics ; Morphology ; mutant ; Mutants ; Mutation ; Mutation - genetics ; Organs ; pea (Pisum sativum) ; Phenotype ; Phylogeny ; Pisum sativum ; Pisum sativum - anatomy & histology ; Pisum sativum - genetics ; Plant Epidermis - cytology ; Plant Leaves - anatomy & histology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; RAD sequencing ; Screens ; stipule ; Transcription ; Zinc ; Zinc finger proteins</subject><ispartof>The New phytologist, 2018-10, Vol.220 (1), p.288-299</ispartof><rights>2018 New Phytologist Trust</rights><rights>2018 The Authors New Phytologist © 2018 New Phytologist Trust</rights><rights>2018 The Authors New Phytologist © 2018 New Phytologist Trust.</rights><rights>Copyright © 2018 New Phytologist Trust</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4106-1f82b5692d899589916bc32536d2dcbf7d9493351da946e6f3067ed81534f62d3</citedby><cites>FETCH-LOGICAL-c4106-1f82b5692d899589916bc32536d2dcbf7d9493351da946e6f3067ed81534f62d3</cites><orcidid>0000-0002-0158-7574 ; 0000-0002-2460-1068</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/90024552$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/90024552$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,782,786,805,1419,1435,27933,27934,45583,45584,46418,46842,58026,58259</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29974468$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moreau, Carol</creatorcontrib><creatorcontrib>Hofer, Julie M. I.</creatorcontrib><creatorcontrib>Eléouët, Morgane</creatorcontrib><creatorcontrib>Sinjushin, Andrey</creatorcontrib><creatorcontrib>Ambrose, Mike</creatorcontrib><creatorcontrib>Skøt, Kirsten</creatorcontrib><creatorcontrib>Blackmore, Tina</creatorcontrib><creatorcontrib>Swain, Martin</creatorcontrib><creatorcontrib>Hegarty, Matthew</creatorcontrib><creatorcontrib>Balanzà, Vicente</creatorcontrib><creatorcontrib>Ferrándiz, Cristina</creatorcontrib><creatorcontrib>Ellis, T. H. Noel</creatorcontrib><title>Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum)</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>Pea (Pisum sativum) is one of relatively few genetically amenable plant species with compound leaves. Pea leaves have a variety of specialized organs: leaflets, tendrils, pulvini and stipules, which enable the identification of mutations that transform or affect distinct parts of the leaf. Characterization of these mutations offers insights into the development and evolution of novel leaf traits. The previously characterized morphological gene Cochleata, conferring stipule identity, was known to interact with Stipules reduced (St), which conditions stipule size in pea, but the St gene remained unknown.
Here we analysed Fast Neutron irradiated pea mutants by restriction site associated DNA sequencing.
We identified St as a gene encoding a C2H2 zinc finger transcription factor that is regulated by Cochleata. St regulates both cell division and cell expansion in the stipule.
Our approach shows how systematic genome-wide screens can be used successfully for the analysis of traits in species for which whole genome sequences are not available.</description><subject>Body organs</subject><subject>C2H2 zinc finger</subject><subject>Cell division</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA sequences</subject><subject>DNA sequencing</subject><subject>Fast Neutron</subject><subject>Fast neutrons</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes, Plant</subject><subject>Genetic Association Studies</subject><subject>Genomes</subject><subject>Identification</subject><subject>leaf</subject><subject>Leaves</subject><subject>Medicago - genetics</subject><subject>Morphology</subject><subject>mutant</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Mutation - genetics</subject><subject>Organs</subject><subject>pea (Pisum sativum)</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>Pisum sativum</subject><subject>Pisum sativum - anatomy & histology</subject><subject>Pisum sativum - genetics</subject><subject>Plant Epidermis - cytology</subject><subject>Plant Leaves - anatomy & histology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>RAD sequencing</subject><subject>Screens</subject><subject>stipule</subject><subject>Transcription</subject><subject>Zinc</subject><subject>Zinc finger proteins</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kV1rFTEQhoMo9li98AcoAW9a6Lb5nE0upagtlFpRwbtlz2bS5rC7WZNd5fx70562F0IHhrl55mF4h5C3nB3zUifjdHPMtTDwjKy4AlsZLuvnZMWYMBUo-LVHXuW8YYxZDeIl2RPW1kqBWZFv5w7HOfjQtXOII42efp_DtPSYaUK3dOiOaEt7bD0dYppuYh-vt_QaR6RhpBO29OAq5GWguQj-LMPha_LCt33GN_dzn_z8_OnH6Vl18fXL-enHi6pTnEHFvRFrDVY4Y60uzWHdSaElOOG6ta-dVVZKzV1rFSB4yaBGZ7iWyoNwcp8c7LxTir8XzHMzhNxh37cjxiU3goGqAYxkBf3wH7qJSxrLdYWymtcG7C11uKO6FHNO6JsphaFN24az5jbnpuTc3OVc2Pf3xmU9oHskH4ItwMkO-Bt63D5tai6vzh6U73YbmzzH9LhhyxOV1kL-AxtCjmI</recordid><startdate>20181001</startdate><enddate>20181001</enddate><creator>Moreau, Carol</creator><creator>Hofer, Julie M. I.</creator><creator>Eléouët, Morgane</creator><creator>Sinjushin, Andrey</creator><creator>Ambrose, Mike</creator><creator>Skøt, Kirsten</creator><creator>Blackmore, Tina</creator><creator>Swain, Martin</creator><creator>Hegarty, Matthew</creator><creator>Balanzà, Vicente</creator><creator>Ferrándiz, Cristina</creator><creator>Ellis, T. H. Noel</creator><general>New Phytologist Trust</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0158-7574</orcidid><orcidid>https://orcid.org/0000-0002-2460-1068</orcidid></search><sort><creationdate>20181001</creationdate><title>Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum)</title><author>Moreau, Carol ; Hofer, Julie M. I. ; Eléouët, Morgane ; Sinjushin, Andrey ; Ambrose, Mike ; Skøt, Kirsten ; Blackmore, Tina ; Swain, Martin ; Hegarty, Matthew ; Balanzà, Vicente ; Ferrándiz, Cristina ; Ellis, T. H. Noel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4106-1f82b5692d899589916bc32536d2dcbf7d9493351da946e6f3067ed81534f62d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Body organs</topic><topic>C2H2 zinc finger</topic><topic>Cell division</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA sequences</topic><topic>DNA sequencing</topic><topic>Fast Neutron</topic><topic>Fast neutrons</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes, Plant</topic><topic>Genetic Association Studies</topic><topic>Genomes</topic><topic>Identification</topic><topic>leaf</topic><topic>Leaves</topic><topic>Medicago - genetics</topic><topic>Morphology</topic><topic>mutant</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Mutation - genetics</topic><topic>Organs</topic><topic>pea (Pisum sativum)</topic><topic>Phenotype</topic><topic>Phylogeny</topic><topic>Pisum sativum</topic><topic>Pisum sativum - anatomy & histology</topic><topic>Pisum sativum - genetics</topic><topic>Plant Epidermis - cytology</topic><topic>Plant Leaves - anatomy & histology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>RAD sequencing</topic><topic>Screens</topic><topic>stipule</topic><topic>Transcription</topic><topic>Zinc</topic><topic>Zinc finger proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moreau, Carol</creatorcontrib><creatorcontrib>Hofer, Julie M. I.</creatorcontrib><creatorcontrib>Eléouët, Morgane</creatorcontrib><creatorcontrib>Sinjushin, Andrey</creatorcontrib><creatorcontrib>Ambrose, Mike</creatorcontrib><creatorcontrib>Skøt, Kirsten</creatorcontrib><creatorcontrib>Blackmore, Tina</creatorcontrib><creatorcontrib>Swain, Martin</creatorcontrib><creatorcontrib>Hegarty, Matthew</creatorcontrib><creatorcontrib>Balanzà, Vicente</creatorcontrib><creatorcontrib>Ferrándiz, Cristina</creatorcontrib><creatorcontrib>Ellis, T. H. Noel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moreau, Carol</au><au>Hofer, Julie M. I.</au><au>Eléouët, Morgane</au><au>Sinjushin, Andrey</au><au>Ambrose, Mike</au><au>Skøt, Kirsten</au><au>Blackmore, Tina</au><au>Swain, Martin</au><au>Hegarty, Matthew</au><au>Balanzà, Vicente</au><au>Ferrándiz, Cristina</au><au>Ellis, T. H. Noel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum)</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2018-10-01</date><risdate>2018</risdate><volume>220</volume><issue>1</issue><spage>288</spage><epage>299</epage><pages>288-299</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Pea (Pisum sativum) is one of relatively few genetically amenable plant species with compound leaves. Pea leaves have a variety of specialized organs: leaflets, tendrils, pulvini and stipules, which enable the identification of mutations that transform or affect distinct parts of the leaf. Characterization of these mutations offers insights into the development and evolution of novel leaf traits. The previously characterized morphological gene Cochleata, conferring stipule identity, was known to interact with Stipules reduced (St), which conditions stipule size in pea, but the St gene remained unknown.
Here we analysed Fast Neutron irradiated pea mutants by restriction site associated DNA sequencing.
We identified St as a gene encoding a C2H2 zinc finger transcription factor that is regulated by Cochleata. St regulates both cell division and cell expansion in the stipule.
Our approach shows how systematic genome-wide screens can be used successfully for the analysis of traits in species for which whole genome sequences are not available.</abstract><cop>England</cop><pub>New Phytologist Trust</pub><pmid>29974468</pmid><doi>10.1111/nph.15286</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-0158-7574</orcidid><orcidid>https://orcid.org/0000-0002-2460-1068</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-646X |
| ispartof | The New phytologist, 2018-10, Vol.220 (1), p.288-299 |
| issn | 0028-646X 1469-8137 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2064766830 |
| source | Wiley-Blackwell Journals; Wiley-Blackwell Backfiles (Open access); MEDLINE; JSTOR; EZB Electronic Journals Library |
| subjects | Body organs C2H2 zinc finger Cell division Deoxyribonucleic acid DNA DNA sequences DNA sequencing Fast Neutron Fast neutrons Gene Expression Regulation, Plant Genes, Plant Genetic Association Studies Genomes Identification leaf Leaves Medicago - genetics Morphology mutant Mutants Mutation Mutation - genetics Organs pea (Pisum sativum) Phenotype Phylogeny Pisum sativum Pisum sativum - anatomy & histology Pisum sativum - genetics Plant Epidermis - cytology Plant Leaves - anatomy & histology Plant Proteins - genetics Plant Proteins - metabolism RAD sequencing Screens stipule Transcription Zinc Zinc finger proteins |
| title | Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-11-29T18%3A26%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20Stipules%20reduced,%20a%20leaf%20morphology%20gene%20in%20pea%20(Pisum%20sativum)&rft.jtitle=The%20New%20phytologist&rft.au=Moreau,%20Carol&rft.date=2018-10-01&rft.volume=220&rft.issue=1&rft.spage=288&rft.epage=299&rft.pages=288-299&rft.issn=0028-646X&rft.eissn=1469-8137&rft_id=info:doi/10.1111/nph.15286&rft_dat=%3Cjstor_proqu%3E90024552%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2095178690&rft_id=info:pmid/29974468&rft_jstor_id=90024552&rfr_iscdi=true |