Subcellular localization and expression of E-cadherin and SNAIL are relevant since early stages of oral carcinogenesis
The biological process of epithelial-to-mesenchymal transition (EMT) has been studied in oral squamous cell carcinoma (OSCC) metastasis, but it is rarely evaluated at several stages of oral carcinogenesis. This study aimed to analyze the presence of SNAIL and E-cadherin proteins, markers of EMT, in...
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| Veröffentlicht in: | Pathology, research and practice research and practice, 2018-08, Vol.214 (8), p.1185-1191 |
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| creator | Lopes, Nathália Martins Xavier, Flávia Caló Aquino Ortiz, Rafael Carneiro Amôr, Nádia Ghinelli Garlet, Gustavo Pompermaier Lara, Vanessa Soares Batista, Aline Carvalho Costa, Nádia Lago Rodini, Camila Oliveira |
| description | The biological process of epithelial-to-mesenchymal transition (EMT) has been studied in oral squamous cell carcinoma (OSCC) metastasis, but it is rarely evaluated at several stages of oral carcinogenesis. This study aimed to analyze the presence of SNAIL and E-cadherin proteins, markers of EMT, in the development and progression of OSCC, evaluating excised specimens of potentially malignant lesions (oral leukoplakia with and without dysplasia-OL and OLD, respectively), tumor tissues (OSCC), metastatic lymph nodes (LN), and normal oral mucosa (NOM) by immunohistochemistry, considering subcellular localization. Additionally, SNAIL and E-cadherin transcripts were evaluated in vitro by qPCR, using SCC-9 cell line in comparison to human keratinocytes (HPEC). There was a significant increase in nuclear expression of SNAIL from NOM to OLD followed by a noticeable decrease in nuclear expression accompanied by increased cytoplasmic expression in OSCC (p |
| doi_str_mv | 10.1016/j.prp.2018.06.004 |
| format | Article |
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This study aimed to analyze the presence of SNAIL and E-cadherin proteins, markers of EMT, in the development and progression of OSCC, evaluating excised specimens of potentially malignant lesions (oral leukoplakia with and without dysplasia-OL and OLD, respectively), tumor tissues (OSCC), metastatic lymph nodes (LN), and normal oral mucosa (NOM) by immunohistochemistry, considering subcellular localization. Additionally, SNAIL and E-cadherin transcripts were evaluated in vitro by qPCR, using SCC-9 cell line in comparison to human keratinocytes (HPEC). There was a significant increase in nuclear expression of SNAIL from NOM to OLD followed by a noticeable decrease in nuclear expression accompanied by increased cytoplasmic expression in OSCC (p<0.05). The E-cadherin cytoplasmic expression was remarkable and statistically significant higher in OSCC and LN, both compared to NOM (p< 0.0001), OL (p<0.01) and OLD (p< 0.0001 and p<0.001, respectively). In vitro, E-cadherin and SNAIL transcripts were lower in SCC-9 compared to HPEC cells, although only the decrease of E-cadherin was statistically significant (p<0.05). Regarding the association of E-cadherin and SNAIL expression with the clinical findings, the analysis revealed an association between the cytoplasmic expression of SNAIL and the invasion pattern (p=0.05) in OSCC. The increased nuclear SNAIL expression may be characteristic of OLD, and the presence of E-cadherin in cell cytoplasm a marker of transformation to malignancy of potentially malignant oral leukoplakias into OSCC.]]></description><identifier>ISSN: 0344-0338</identifier><identifier>EISSN: 1618-0631</identifier><identifier>DOI: 10.1016/j.prp.2018.06.004</identifier><identifier>PMID: 29970306</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Adult ; Aged ; Antigens, CD ; Cadherins - metabolism ; Carcinogenesis - metabolism ; Carcinogenesis - pathology ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; Cell Nucleus - metabolism ; Cytoplasm - metabolism ; Epithelial dysplasia ; Epithelial-Mesenchymal Transition - physiology ; Epithelial-To-Mesenchymal transition ; Female ; Head and Neck Neoplasms - metabolism ; Head and Neck Neoplasms - pathology ; Humans ; Male ; Middle Aged ; Mouth Neoplasms - metabolism ; Mouth Neoplasms - pathology ; Oral carcinogenesis ; Oral leukoplakia ; Oral squamous cell carcinoma ; Precancerous Conditions - metabolism ; Precancerous Conditions - pathology ; Snail Family Transcription Factors - metabolism ; Squamous Cell Carcinoma of Head and Neck</subject><ispartof>Pathology, research and practice, 2018-08, Vol.214 (8), p.1185-1191</ispartof><rights>2018 Elsevier GmbH</rights><rights>Copyright © 2018 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-28c94ffa6a2ceec45e1b0ee44fdbe799564272a57c94adcc46ea2a62465cfe3</citedby><cites>FETCH-LOGICAL-c353t-28c94ffa6a2ceec45e1b0ee44fdbe799564272a57c94adcc46ea2a62465cfe3</cites><orcidid>0000-0003-0198-5828 ; 0000-0002-5071-8382 ; 0000-0002-5616-523X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.prp.2018.06.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29970306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lopes, Nathália Martins</creatorcontrib><creatorcontrib>Xavier, Flávia Caló Aquino</creatorcontrib><creatorcontrib>Ortiz, Rafael Carneiro</creatorcontrib><creatorcontrib>Amôr, Nádia Ghinelli</creatorcontrib><creatorcontrib>Garlet, Gustavo Pompermaier</creatorcontrib><creatorcontrib>Lara, Vanessa Soares</creatorcontrib><creatorcontrib>Batista, Aline Carvalho</creatorcontrib><creatorcontrib>Costa, Nádia Lago</creatorcontrib><creatorcontrib>Rodini, Camila Oliveira</creatorcontrib><title>Subcellular localization and expression of E-cadherin and SNAIL are relevant since early stages of oral carcinogenesis</title><title>Pathology, research and practice</title><addtitle>Pathol Res Pract</addtitle><description><![CDATA[The biological process of epithelial-to-mesenchymal transition (EMT) has been studied in oral squamous cell carcinoma (OSCC) metastasis, but it is rarely evaluated at several stages of oral carcinogenesis. This study aimed to analyze the presence of SNAIL and E-cadherin proteins, markers of EMT, in the development and progression of OSCC, evaluating excised specimens of potentially malignant lesions (oral leukoplakia with and without dysplasia-OL and OLD, respectively), tumor tissues (OSCC), metastatic lymph nodes (LN), and normal oral mucosa (NOM) by immunohistochemistry, considering subcellular localization. Additionally, SNAIL and E-cadherin transcripts were evaluated in vitro by qPCR, using SCC-9 cell line in comparison to human keratinocytes (HPEC). There was a significant increase in nuclear expression of SNAIL from NOM to OLD followed by a noticeable decrease in nuclear expression accompanied by increased cytoplasmic expression in OSCC (p<0.05). The E-cadherin cytoplasmic expression was remarkable and statistically significant higher in OSCC and LN, both compared to NOM (p< 0.0001), OL (p<0.01) and OLD (p< 0.0001 and p<0.001, respectively). In vitro, E-cadherin and SNAIL transcripts were lower in SCC-9 compared to HPEC cells, although only the decrease of E-cadherin was statistically significant (p<0.05). Regarding the association of E-cadherin and SNAIL expression with the clinical findings, the analysis revealed an association between the cytoplasmic expression of SNAIL and the invasion pattern (p=0.05) in OSCC. The increased nuclear SNAIL expression may be characteristic of OLD, and the presence of E-cadherin in cell cytoplasm a marker of transformation to malignancy of potentially malignant oral leukoplakias into OSCC.]]></description><subject>Adult</subject><subject>Aged</subject><subject>Antigens, CD</subject><subject>Cadherins - metabolism</subject><subject>Carcinogenesis - metabolism</subject><subject>Carcinogenesis - pathology</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytoplasm - metabolism</subject><subject>Epithelial dysplasia</subject><subject>Epithelial-Mesenchymal Transition - physiology</subject><subject>Epithelial-To-Mesenchymal transition</subject><subject>Female</subject><subject>Head and Neck Neoplasms - metabolism</subject><subject>Head and Neck Neoplasms - pathology</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mouth Neoplasms - metabolism</subject><subject>Mouth Neoplasms - pathology</subject><subject>Oral carcinogenesis</subject><subject>Oral leukoplakia</subject><subject>Oral squamous cell carcinoma</subject><subject>Precancerous Conditions - metabolism</subject><subject>Precancerous Conditions - pathology</subject><subject>Snail Family Transcription Factors - metabolism</subject><subject>Squamous Cell Carcinoma of Head and Neck</subject><issn>0344-0338</issn><issn>1618-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAQhi0EokvbB-CCfOSSMHYcJxGnqipQadUelrs160yKV9442MmK8vQ42sKR08jj7_-l-Rh7L6AUIPSnQznFqZQg2hJ0CaBesY3Qoi1AV-I120ClVAFV1V6wdykdAKABJd6yC9l1DVSgN-y0W_aWvF88Ru6DRe9-4-zCyHHsOf2aIqW0PsPA7wqL_Q-K7vy5e7i533KMxCN5OuE48-RGS5ww-meeZnyitOZCRM8tRuvG8EQjJZeu2JsBfaLrl3nJdl_uvt9-K7aPX-9vb7aFrepqLmRrOzUMqFFaIqtqEnsgUmro99R0Xa2VbCTWTcawt1ZpQolaKl3bgapL9vHcOsXwc6E0m6NL67U4UliSkZDzSohWZlScURtDSpEGM0V3xPhsBJhVtjnkzWRW2Qa0ybJz5sNL_bI_Uv8v8dduBj6fAconnhxFk6yjrKh3kexs-uD-U_8HO42R_w</recordid><startdate>201808</startdate><enddate>201808</enddate><creator>Lopes, Nathália Martins</creator><creator>Xavier, Flávia Caló Aquino</creator><creator>Ortiz, Rafael Carneiro</creator><creator>Amôr, Nádia Ghinelli</creator><creator>Garlet, Gustavo Pompermaier</creator><creator>Lara, Vanessa Soares</creator><creator>Batista, Aline Carvalho</creator><creator>Costa, Nádia Lago</creator><creator>Rodini, Camila Oliveira</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0198-5828</orcidid><orcidid>https://orcid.org/0000-0002-5071-8382</orcidid><orcidid>https://orcid.org/0000-0002-5616-523X</orcidid></search><sort><creationdate>201808</creationdate><title>Subcellular localization and expression of E-cadherin and SNAIL are relevant since early stages of oral carcinogenesis</title><author>Lopes, Nathália Martins ; Xavier, Flávia Caló Aquino ; Ortiz, Rafael Carneiro ; Amôr, Nádia Ghinelli ; Garlet, Gustavo Pompermaier ; Lara, Vanessa Soares ; Batista, Aline Carvalho ; Costa, Nádia Lago ; Rodini, Camila Oliveira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-28c94ffa6a2ceec45e1b0ee44fdbe799564272a57c94adcc46ea2a62465cfe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Antigens, CD</topic><topic>Cadherins - metabolism</topic><topic>Carcinogenesis - metabolism</topic><topic>Carcinogenesis - pathology</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Cell Nucleus - metabolism</topic><topic>Cytoplasm - metabolism</topic><topic>Epithelial dysplasia</topic><topic>Epithelial-Mesenchymal Transition - physiology</topic><topic>Epithelial-To-Mesenchymal transition</topic><topic>Female</topic><topic>Head and Neck Neoplasms - metabolism</topic><topic>Head and Neck Neoplasms - pathology</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Mouth Neoplasms - metabolism</topic><topic>Mouth Neoplasms - pathology</topic><topic>Oral carcinogenesis</topic><topic>Oral leukoplakia</topic><topic>Oral squamous cell carcinoma</topic><topic>Precancerous Conditions - metabolism</topic><topic>Precancerous Conditions - pathology</topic><topic>Snail Family Transcription Factors - metabolism</topic><topic>Squamous Cell Carcinoma of Head and Neck</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lopes, Nathália Martins</creatorcontrib><creatorcontrib>Xavier, Flávia Caló Aquino</creatorcontrib><creatorcontrib>Ortiz, Rafael Carneiro</creatorcontrib><creatorcontrib>Amôr, Nádia Ghinelli</creatorcontrib><creatorcontrib>Garlet, Gustavo Pompermaier</creatorcontrib><creatorcontrib>Lara, Vanessa Soares</creatorcontrib><creatorcontrib>Batista, Aline Carvalho</creatorcontrib><creatorcontrib>Costa, Nádia Lago</creatorcontrib><creatorcontrib>Rodini, Camila Oliveira</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pathology, research and practice</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lopes, Nathália Martins</au><au>Xavier, Flávia Caló Aquino</au><au>Ortiz, Rafael Carneiro</au><au>Amôr, Nádia Ghinelli</au><au>Garlet, Gustavo Pompermaier</au><au>Lara, Vanessa Soares</au><au>Batista, Aline Carvalho</au><au>Costa, Nádia Lago</au><au>Rodini, Camila Oliveira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Subcellular localization and expression of E-cadherin and SNAIL are relevant since early stages of oral carcinogenesis</atitle><jtitle>Pathology, research and practice</jtitle><addtitle>Pathol Res Pract</addtitle><date>2018-08</date><risdate>2018</risdate><volume>214</volume><issue>8</issue><spage>1185</spage><epage>1191</epage><pages>1185-1191</pages><issn>0344-0338</issn><eissn>1618-0631</eissn><abstract><![CDATA[The biological process of epithelial-to-mesenchymal transition (EMT) has been studied in oral squamous cell carcinoma (OSCC) metastasis, but it is rarely evaluated at several stages of oral carcinogenesis. This study aimed to analyze the presence of SNAIL and E-cadherin proteins, markers of EMT, in the development and progression of OSCC, evaluating excised specimens of potentially malignant lesions (oral leukoplakia with and without dysplasia-OL and OLD, respectively), tumor tissues (OSCC), metastatic lymph nodes (LN), and normal oral mucosa (NOM) by immunohistochemistry, considering subcellular localization. Additionally, SNAIL and E-cadherin transcripts were evaluated in vitro by qPCR, using SCC-9 cell line in comparison to human keratinocytes (HPEC). There was a significant increase in nuclear expression of SNAIL from NOM to OLD followed by a noticeable decrease in nuclear expression accompanied by increased cytoplasmic expression in OSCC (p<0.05). The E-cadherin cytoplasmic expression was remarkable and statistically significant higher in OSCC and LN, both compared to NOM (p< 0.0001), OL (p<0.01) and OLD (p< 0.0001 and p<0.001, respectively). In vitro, E-cadherin and SNAIL transcripts were lower in SCC-9 compared to HPEC cells, although only the decrease of E-cadherin was statistically significant (p<0.05). Regarding the association of E-cadherin and SNAIL expression with the clinical findings, the analysis revealed an association between the cytoplasmic expression of SNAIL and the invasion pattern (p=0.05) in OSCC. The increased nuclear SNAIL expression may be characteristic of OLD, and the presence of E-cadherin in cell cytoplasm a marker of transformation to malignancy of potentially malignant oral leukoplakias into OSCC.]]></abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>29970306</pmid><doi>10.1016/j.prp.2018.06.004</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0198-5828</orcidid><orcidid>https://orcid.org/0000-0002-5071-8382</orcidid><orcidid>https://orcid.org/0000-0002-5616-523X</orcidid></addata></record> |
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| ispartof | Pathology, research and practice, 2018-08, Vol.214 (8), p.1185-1191 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Aged Antigens, CD Cadherins - metabolism Carcinogenesis - metabolism Carcinogenesis - pathology Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Cell Nucleus - metabolism Cytoplasm - metabolism Epithelial dysplasia Epithelial-Mesenchymal Transition - physiology Epithelial-To-Mesenchymal transition Female Head and Neck Neoplasms - metabolism Head and Neck Neoplasms - pathology Humans Male Middle Aged Mouth Neoplasms - metabolism Mouth Neoplasms - pathology Oral carcinogenesis Oral leukoplakia Oral squamous cell carcinoma Precancerous Conditions - metabolism Precancerous Conditions - pathology Snail Family Transcription Factors - metabolism Squamous Cell Carcinoma of Head and Neck |
| title | Subcellular localization and expression of E-cadherin and SNAIL are relevant since early stages of oral carcinogenesis |
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