Visualizing Energy Charge in Breast Carcinoma Tissues by MALDI Mass-spectrometry Imaging Profiles of Low-molecular-weight Metabolites
Metabolomics is widely used for biomarker discovery, but conventional mass-spectrometry extraction procedures lose the spatial localization of metabolites. In this study, we directly analyzed breast carcinoma tissues embedded in frozen tissue microarrays (fTMAs) using MALDI mass-spectrometry imaging...
Gespeichert in:
| Veröffentlicht in: | Anticancer research 2018-07, Vol.38 (7), p.4267-4272 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4272 |
|---|---|
| container_issue | 7 |
| container_start_page | 4267 |
| container_title | Anticancer research |
| container_volume | 38 |
| creator | Torata, Nobuhiro Kubo, Makoto Miura, Daisuke Ohuchida, Kenoki Mizuuchi, Yusuke Fujimura, Yoshinori Hayakawa, Eisuke Kai, Masaya Oda, Yoshinao Mizumoto, Kazuhiro Hashizume, Makoto Nakamura, Masafumi |
| description | Metabolomics is widely used for biomarker discovery, but conventional mass-spectrometry extraction procedures lose the spatial localization of metabolites. In this study, we directly analyzed breast carcinoma tissues embedded in frozen tissue microarrays (fTMAs) using MALDI mass-spectrometry imaging (MALDI-MSI).
A total of 119 breast tissues (84 carcinoma and 35 normal) were used. MSI data were extracted from each tissue.
Overall, 185 of 1,915 peaks which were commonly detected in 60% of target areas were subjected to further analysis. One hundred and fifty-two peaks of carcinoma showed significantly higher intensity than normal. Comparing metabolite profiles from carcinoma and normal tissues, energy charge (EC) and the sum of adenosine phosphate compound (AXP) indicated significantly higher intensities in cancerous tissues than normal. But comparisons of EC and AXP among lymph node metastasis, tumor size and tumor subtypes indicated no significant differences.
Breast carcinoma tissues had higher EC and AXP values than normal. MALDI-MSI could be a tool for characterizing breast carcinoma. |
| doi_str_mv | 10.21873/anticanres.12723 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2064240167</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2138074736</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-7a317d979fa6cb248d09b1ed1fcdbb2f9df5c8a9683906513e03004cb25c17e3</originalsourceid><addsrcrecordid>eNpdkcFO3DAURa2KqkxpP6CbyhKbbgLPdmLHSxhoO9KMYDHqNnKcl2CUxIPtCE33_DcpUJBYvc25V_fpEPKNwQlnpRKnZkzOmjFgPGFccfGBLJjSLFOFgAOyAF5ApgCKQ_I5xlsAKXUpPpFDrrWCQsKCPPxxcTK9--vGjl6OGLo9Xd6Y0CF1Iz0PaGKiSxOsG_1g6NbFOGGk9Z5uztYXK7oxMWZxhzYFP2AKe7oaTPev7Dr41vUz61u69vfZ4Hu0U29Cdo-uu0l0g8nUvncJ4xfysTV9xK8v94hsf15ul7-z9dWv1fJsndlclClTRjDVaKVbI23N87IBXTNsWGubuuatbtrClkbLUmiQBRMIAiCf0cIyheKI_Hiu3QV_N7-RqsFFi31vRvRTrDjInOfApJrR43forZ_COI-rOBMlqFwJOVPsmbLBxxiwrXbBDSbsKwbVk6LqTVH1pGjOfH9pnuoBm9fEfyfiEY6zkMY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2138074736</pqid></control><display><type>article</type><title>Visualizing Energy Charge in Breast Carcinoma Tissues by MALDI Mass-spectrometry Imaging Profiles of Low-molecular-weight Metabolites</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Torata, Nobuhiro ; Kubo, Makoto ; Miura, Daisuke ; Ohuchida, Kenoki ; Mizuuchi, Yusuke ; Fujimura, Yoshinori ; Hayakawa, Eisuke ; Kai, Masaya ; Oda, Yoshinao ; Mizumoto, Kazuhiro ; Hashizume, Makoto ; Nakamura, Masafumi</creator><creatorcontrib>Torata, Nobuhiro ; Kubo, Makoto ; Miura, Daisuke ; Ohuchida, Kenoki ; Mizuuchi, Yusuke ; Fujimura, Yoshinori ; Hayakawa, Eisuke ; Kai, Masaya ; Oda, Yoshinao ; Mizumoto, Kazuhiro ; Hashizume, Makoto ; Nakamura, Masafumi</creatorcontrib><description>Metabolomics is widely used for biomarker discovery, but conventional mass-spectrometry extraction procedures lose the spatial localization of metabolites. In this study, we directly analyzed breast carcinoma tissues embedded in frozen tissue microarrays (fTMAs) using MALDI mass-spectrometry imaging (MALDI-MSI).
A total of 119 breast tissues (84 carcinoma and 35 normal) were used. MSI data were extracted from each tissue.
Overall, 185 of 1,915 peaks which were commonly detected in 60% of target areas were subjected to further analysis. One hundred and fifty-two peaks of carcinoma showed significantly higher intensity than normal. Comparing metabolite profiles from carcinoma and normal tissues, energy charge (EC) and the sum of adenosine phosphate compound (AXP) indicated significantly higher intensities in cancerous tissues than normal. But comparisons of EC and AXP among lymph node metastasis, tumor size and tumor subtypes indicated no significant differences.
Breast carcinoma tissues had higher EC and AXP values than normal. MALDI-MSI could be a tool for characterizing breast carcinoma.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>DOI: 10.21873/anticanres.12723</identifier><identifier>PMID: 29970560</identifier><language>eng</language><publisher>Greece: International Institute of Anticancer Research</publisher><subject>Adenosine ; Adult ; Aged ; Biomarkers ; Breast ; Breast cancer ; Breast carcinoma ; Breast Neoplasms - diagnostic imaging ; Breast Neoplasms - metabolism ; Energy charge ; Female ; Humans ; Localization ; Low molecular weights ; Lymph nodes ; Male ; Metabolites ; Metabolomics ; Metabolomics - methods ; Metastases ; Middle Aged ; Scientific imaging ; Spatial discrimination ; Spectrometry ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Spectroscopy ; Target detection ; Tissues ; Tumors</subject><ispartof>Anticancer research, 2018-07, Vol.38 (7), p.4267-4272</ispartof><rights>Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.</rights><rights>Copyright International Institute of Anticancer Research Jul 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-7a317d979fa6cb248d09b1ed1fcdbb2f9df5c8a9683906513e03004cb25c17e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29970560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Torata, Nobuhiro</creatorcontrib><creatorcontrib>Kubo, Makoto</creatorcontrib><creatorcontrib>Miura, Daisuke</creatorcontrib><creatorcontrib>Ohuchida, Kenoki</creatorcontrib><creatorcontrib>Mizuuchi, Yusuke</creatorcontrib><creatorcontrib>Fujimura, Yoshinori</creatorcontrib><creatorcontrib>Hayakawa, Eisuke</creatorcontrib><creatorcontrib>Kai, Masaya</creatorcontrib><creatorcontrib>Oda, Yoshinao</creatorcontrib><creatorcontrib>Mizumoto, Kazuhiro</creatorcontrib><creatorcontrib>Hashizume, Makoto</creatorcontrib><creatorcontrib>Nakamura, Masafumi</creatorcontrib><title>Visualizing Energy Charge in Breast Carcinoma Tissues by MALDI Mass-spectrometry Imaging Profiles of Low-molecular-weight Metabolites</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Metabolomics is widely used for biomarker discovery, but conventional mass-spectrometry extraction procedures lose the spatial localization of metabolites. In this study, we directly analyzed breast carcinoma tissues embedded in frozen tissue microarrays (fTMAs) using MALDI mass-spectrometry imaging (MALDI-MSI).
A total of 119 breast tissues (84 carcinoma and 35 normal) were used. MSI data were extracted from each tissue.
Overall, 185 of 1,915 peaks which were commonly detected in 60% of target areas were subjected to further analysis. One hundred and fifty-two peaks of carcinoma showed significantly higher intensity than normal. Comparing metabolite profiles from carcinoma and normal tissues, energy charge (EC) and the sum of adenosine phosphate compound (AXP) indicated significantly higher intensities in cancerous tissues than normal. But comparisons of EC and AXP among lymph node metastasis, tumor size and tumor subtypes indicated no significant differences.
Breast carcinoma tissues had higher EC and AXP values than normal. MALDI-MSI could be a tool for characterizing breast carcinoma.</description><subject>Adenosine</subject><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers</subject><subject>Breast</subject><subject>Breast cancer</subject><subject>Breast carcinoma</subject><subject>Breast Neoplasms - diagnostic imaging</subject><subject>Breast Neoplasms - metabolism</subject><subject>Energy charge</subject><subject>Female</subject><subject>Humans</subject><subject>Localization</subject><subject>Low molecular weights</subject><subject>Lymph nodes</subject><subject>Male</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Metabolomics - methods</subject><subject>Metastases</subject><subject>Middle Aged</subject><subject>Scientific imaging</subject><subject>Spatial discrimination</subject><subject>Spectrometry</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Spectroscopy</subject><subject>Target detection</subject><subject>Tissues</subject><subject>Tumors</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkcFO3DAURa2KqkxpP6CbyhKbbgLPdmLHSxhoO9KMYDHqNnKcl2CUxIPtCE33_DcpUJBYvc25V_fpEPKNwQlnpRKnZkzOmjFgPGFccfGBLJjSLFOFgAOyAF5ApgCKQ_I5xlsAKXUpPpFDrrWCQsKCPPxxcTK9--vGjl6OGLo9Xd6Y0CF1Iz0PaGKiSxOsG_1g6NbFOGGk9Z5uztYXK7oxMWZxhzYFP2AKe7oaTPev7Dr41vUz61u69vfZ4Hu0U29Cdo-uu0l0g8nUvncJ4xfysTV9xK8v94hsf15ul7-z9dWv1fJsndlclClTRjDVaKVbI23N87IBXTNsWGubuuatbtrClkbLUmiQBRMIAiCf0cIyheKI_Hiu3QV_N7-RqsFFi31vRvRTrDjInOfApJrR43forZ_COI-rOBMlqFwJOVPsmbLBxxiwrXbBDSbsKwbVk6LqTVH1pGjOfH9pnuoBm9fEfyfiEY6zkMY</recordid><startdate>201807</startdate><enddate>201807</enddate><creator>Torata, Nobuhiro</creator><creator>Kubo, Makoto</creator><creator>Miura, Daisuke</creator><creator>Ohuchida, Kenoki</creator><creator>Mizuuchi, Yusuke</creator><creator>Fujimura, Yoshinori</creator><creator>Hayakawa, Eisuke</creator><creator>Kai, Masaya</creator><creator>Oda, Yoshinao</creator><creator>Mizumoto, Kazuhiro</creator><creator>Hashizume, Makoto</creator><creator>Nakamura, Masafumi</creator><general>International Institute of Anticancer Research</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201807</creationdate><title>Visualizing Energy Charge in Breast Carcinoma Tissues by MALDI Mass-spectrometry Imaging Profiles of Low-molecular-weight Metabolites</title><author>Torata, Nobuhiro ; Kubo, Makoto ; Miura, Daisuke ; Ohuchida, Kenoki ; Mizuuchi, Yusuke ; Fujimura, Yoshinori ; Hayakawa, Eisuke ; Kai, Masaya ; Oda, Yoshinao ; Mizumoto, Kazuhiro ; Hashizume, Makoto ; Nakamura, Masafumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-7a317d979fa6cb248d09b1ed1fcdbb2f9df5c8a9683906513e03004cb25c17e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adenosine</topic><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers</topic><topic>Breast</topic><topic>Breast cancer</topic><topic>Breast carcinoma</topic><topic>Breast Neoplasms - diagnostic imaging</topic><topic>Breast Neoplasms - metabolism</topic><topic>Energy charge</topic><topic>Female</topic><topic>Humans</topic><topic>Localization</topic><topic>Low molecular weights</topic><topic>Lymph nodes</topic><topic>Male</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Metabolomics - methods</topic><topic>Metastases</topic><topic>Middle Aged</topic><topic>Scientific imaging</topic><topic>Spatial discrimination</topic><topic>Spectrometry</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Spectroscopy</topic><topic>Target detection</topic><topic>Tissues</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Torata, Nobuhiro</creatorcontrib><creatorcontrib>Kubo, Makoto</creatorcontrib><creatorcontrib>Miura, Daisuke</creatorcontrib><creatorcontrib>Ohuchida, Kenoki</creatorcontrib><creatorcontrib>Mizuuchi, Yusuke</creatorcontrib><creatorcontrib>Fujimura, Yoshinori</creatorcontrib><creatorcontrib>Hayakawa, Eisuke</creatorcontrib><creatorcontrib>Kai, Masaya</creatorcontrib><creatorcontrib>Oda, Yoshinao</creatorcontrib><creatorcontrib>Mizumoto, Kazuhiro</creatorcontrib><creatorcontrib>Hashizume, Makoto</creatorcontrib><creatorcontrib>Nakamura, Masafumi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torata, Nobuhiro</au><au>Kubo, Makoto</au><au>Miura, Daisuke</au><au>Ohuchida, Kenoki</au><au>Mizuuchi, Yusuke</au><au>Fujimura, Yoshinori</au><au>Hayakawa, Eisuke</au><au>Kai, Masaya</au><au>Oda, Yoshinao</au><au>Mizumoto, Kazuhiro</au><au>Hashizume, Makoto</au><au>Nakamura, Masafumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Visualizing Energy Charge in Breast Carcinoma Tissues by MALDI Mass-spectrometry Imaging Profiles of Low-molecular-weight Metabolites</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2018-07</date><risdate>2018</risdate><volume>38</volume><issue>7</issue><spage>4267</spage><epage>4272</epage><pages>4267-4272</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Metabolomics is widely used for biomarker discovery, but conventional mass-spectrometry extraction procedures lose the spatial localization of metabolites. In this study, we directly analyzed breast carcinoma tissues embedded in frozen tissue microarrays (fTMAs) using MALDI mass-spectrometry imaging (MALDI-MSI).
A total of 119 breast tissues (84 carcinoma and 35 normal) were used. MSI data were extracted from each tissue.
Overall, 185 of 1,915 peaks which were commonly detected in 60% of target areas were subjected to further analysis. One hundred and fifty-two peaks of carcinoma showed significantly higher intensity than normal. Comparing metabolite profiles from carcinoma and normal tissues, energy charge (EC) and the sum of adenosine phosphate compound (AXP) indicated significantly higher intensities in cancerous tissues than normal. But comparisons of EC and AXP among lymph node metastasis, tumor size and tumor subtypes indicated no significant differences.
Breast carcinoma tissues had higher EC and AXP values than normal. MALDI-MSI could be a tool for characterizing breast carcinoma.</abstract><cop>Greece</cop><pub>International Institute of Anticancer Research</pub><pmid>29970560</pmid><doi>10.21873/anticanres.12723</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0250-7005 |
| ispartof | Anticancer research, 2018-07, Vol.38 (7), p.4267-4272 |
| issn | 0250-7005 1791-7530 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2064240167 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adenosine Adult Aged Biomarkers Breast Breast cancer Breast carcinoma Breast Neoplasms - diagnostic imaging Breast Neoplasms - metabolism Energy charge Female Humans Localization Low molecular weights Lymph nodes Male Metabolites Metabolomics Metabolomics - methods Metastases Middle Aged Scientific imaging Spatial discrimination Spectrometry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Spectroscopy Target detection Tissues Tumors |
| title | Visualizing Energy Charge in Breast Carcinoma Tissues by MALDI Mass-spectrometry Imaging Profiles of Low-molecular-weight Metabolites |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T23%3A03%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Visualizing%20Energy%20Charge%20in%20Breast%20Carcinoma%20Tissues%20by%20MALDI%20Mass-spectrometry%20Imaging%20Profiles%20of%20Low-molecular-weight%20Metabolites&rft.jtitle=Anticancer%20research&rft.au=Torata,%20Nobuhiro&rft.date=2018-07&rft.volume=38&rft.issue=7&rft.spage=4267&rft.epage=4272&rft.pages=4267-4272&rft.issn=0250-7005&rft.eissn=1791-7530&rft_id=info:doi/10.21873/anticanres.12723&rft_dat=%3Cproquest_cross%3E2138074736%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2138074736&rft_id=info:pmid/29970560&rfr_iscdi=true |