Agrobacterium-mediated transformation of a low glutelin mutant of 'Koshihikari' rice variety using the mutated-acetolactate synthase gene derived from rice genome as a selectable marker
We have developed an efficient rice transformation system that uses only rice genome-derived components. The transgenic 'Koshihikari' rice, low-glutelin mutant a123, is capable of accumulating large amounts of bioactive peptides in the endosperm. Agrobacterium-mediated transformation using...
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| Veröffentlicht in: | Plant cell reports 2007-09, Vol.26 (9), p.1567-1573 |
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| creator | Wakasa, Yuhya Ozawa, Kenjirou Takaiwa, Fumio |
| description | We have developed an efficient rice transformation system that uses only rice genome-derived components. The transgenic 'Koshihikari' rice, low-glutelin mutant a123, is capable of accumulating large amounts of bioactive peptides in the endosperm. Agrobacterium-mediated transformation using the mutated-acetolactate synthase (mALS) gene expressed under the control of the callus-specific promoter (CSP) as a selectable marker was used to introduce GFP and an anti-hypertensive hexapeptide into 'Koshihikari' a123. The CSP:mALS gene cassette confers pyrimidinyl carboxy herbicide resistance to transgenic rice callus, but is not expressed in regenerated plants. Transformation efficiency of transgenic rice line a123 was improved from about 10% to about 30% by modifying callus induction, callus selection and regeneration media conventionally used for rice tissue culture. |
| doi_str_mv | 10.1007/s00299-007-0373-0 |
| format | Article |
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The transgenic 'Koshihikari' rice, low-glutelin mutant a123, is capable of accumulating large amounts of bioactive peptides in the endosperm. Agrobacterium-mediated transformation using the mutated-acetolactate synthase (mALS) gene expressed under the control of the callus-specific promoter (CSP) as a selectable marker was used to introduce GFP and an anti-hypertensive hexapeptide into 'Koshihikari' a123. The CSP:mALS gene cassette confers pyrimidinyl carboxy herbicide resistance to transgenic rice callus, but is not expressed in regenerated plants. Transformation efficiency of transgenic rice line a123 was improved from about 10% to about 30% by modifying callus induction, callus selection and regeneration media conventionally used for rice tissue culture.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-007-0373-0</identifier><identifier>PMID: 17516071</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Berlin/Heidelberg : Springer-Verlag</publisher><subject>acetolactate synthase ; Acetolactate Synthase - genetics ; Agrobacterium ; Biological and medical sciences ; Biotechnology ; Culture Media ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic Markers ; Genetic technics ; Genetic Vectors ; Genome, Plant - genetics ; Glutens - metabolism ; Herbicides ; Koshihikari ; Methods. Procedures. Technologies ; Microbiology ; Mutation - genetics ; Oryza - genetics ; Oryza sativa ; Peptides ; Plants, Genetically Modified ; Rhizobium - physiology ; Rice ; Transformation, Genetic ; Transgenic animals and transgenic plants ; Transgenic plants ; Transgenic rice</subject><ispartof>Plant cell reports, 2007-09, Vol.26 (9), p.1567-1573</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-4a0234679e6b28c51920cd312274604dc26aaf3a2b146d5331f24bdd0f5c4c6b3</citedby><cites>FETCH-LOGICAL-c508t-4a0234679e6b28c51920cd312274604dc26aaf3a2b146d5331f24bdd0f5c4c6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19063211$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17516071$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wakasa, Yuhya</creatorcontrib><creatorcontrib>Ozawa, Kenjirou</creatorcontrib><creatorcontrib>Takaiwa, Fumio</creatorcontrib><title>Agrobacterium-mediated transformation of a low glutelin mutant of 'Koshihikari' rice variety using the mutated-acetolactate synthase gene derived from rice genome as a selectable marker</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>We have developed an efficient rice transformation system that uses only rice genome-derived components. The transgenic 'Koshihikari' rice, low-glutelin mutant a123, is capable of accumulating large amounts of bioactive peptides in the endosperm. Agrobacterium-mediated transformation using the mutated-acetolactate synthase (mALS) gene expressed under the control of the callus-specific promoter (CSP) as a selectable marker was used to introduce GFP and an anti-hypertensive hexapeptide into 'Koshihikari' a123. The CSP:mALS gene cassette confers pyrimidinyl carboxy herbicide resistance to transgenic rice callus, but is not expressed in regenerated plants. Transformation efficiency of transgenic rice line a123 was improved from about 10% to about 30% by modifying callus induction, callus selection and regeneration media conventionally used for rice tissue culture.</description><subject>acetolactate synthase</subject><subject>Acetolactate Synthase - genetics</subject><subject>Agrobacterium</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Culture Media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic Markers</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>Genome, Plant - genetics</subject><subject>Glutens - metabolism</subject><subject>Herbicides</subject><subject>Koshihikari</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Mutation - genetics</subject><subject>Oryza - genetics</subject><subject>Oryza sativa</subject><subject>Peptides</subject><subject>Plants, Genetically Modified</subject><subject>Rhizobium - physiology</subject><subject>Rice</subject><subject>Transformation, Genetic</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><subject>Transgenic rice</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkc1u1TAQhSMEopfCA7ABCwm6CoztxE6WVcWfqMQCKrGLHGeS6zaJi-0U3Ufj7ZiQK1Vig7zw2P7mjI9Olj3n8JYD6HcRQNR1TmUOUsscHmQ7XkiRC5A_HmY70ILnWvPiJHsS4zUAPWr1ODvhuuQKNN9lv8-H4FtjEwa3TPmEnTMJO5aCmWPvw2SS8zPzPTNs9L_YMC4JRzezaUlmTuvD2Rcf927vbkxwZyw4i-yOSkwHtkQ3Dyzt8S9OurmxmPxI8-jE4mFOexORDTgj6-gLdzS6D37aZOjaT8hMpOERR6SudiQtE24wPM0e9WaM-Oy4n2ZXH95_v_iUX379-Pni_DK3JVQpLwwIWShdo2pFZUteC7Cd5ELoQkHRWaGM6aURLS9UV0rJe1G0XQd9aQurWnmavdl0b4P_uWBMzeSixXE0M_olNgKUrBSv_gvyuhQaJBD46h_w2i9hJhNNBbqoaWmC-AbZ4GMM2De3wZHzQ8OhWdNvtvSbtVzTb1bhF0fhpaUk7zuOcRPw-giYaM3YU8jWxXuuJi-Cr9zLjeuNb8wQiLn6JoBLGlZXilz8AcVvw5Q</recordid><startdate>20070901</startdate><enddate>20070901</enddate><creator>Wakasa, Yuhya</creator><creator>Ozawa, Kenjirou</creator><creator>Takaiwa, Fumio</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope></search><sort><creationdate>20070901</creationdate><title>Agrobacterium-mediated transformation of a low glutelin mutant of 'Koshihikari' rice variety using the mutated-acetolactate synthase gene derived from rice genome as a selectable marker</title><author>Wakasa, Yuhya ; Ozawa, Kenjirou ; Takaiwa, Fumio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-4a0234679e6b28c51920cd312274604dc26aaf3a2b146d5331f24bdd0f5c4c6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>acetolactate synthase</topic><topic>Acetolactate Synthase - genetics</topic><topic>Agrobacterium</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Culture Media</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic Markers</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>Genome, Plant - genetics</topic><topic>Glutens - metabolism</topic><topic>Herbicides</topic><topic>Koshihikari</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Mutation - genetics</topic><topic>Oryza - genetics</topic><topic>Oryza sativa</topic><topic>Peptides</topic><topic>Plants, Genetically Modified</topic><topic>Rhizobium - physiology</topic><topic>Rice</topic><topic>Transformation, Genetic</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><topic>Transgenic rice</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wakasa, Yuhya</creatorcontrib><creatorcontrib>Ozawa, Kenjirou</creatorcontrib><creatorcontrib>Takaiwa, Fumio</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wakasa, Yuhya</au><au>Ozawa, Kenjirou</au><au>Takaiwa, Fumio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Agrobacterium-mediated transformation of a low glutelin mutant of 'Koshihikari' rice variety using the mutated-acetolactate synthase gene derived from rice genome as a selectable marker</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>26</volume><issue>9</issue><spage>1567</spage><epage>1573</epage><pages>1567-1573</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>We have developed an efficient rice transformation system that uses only rice genome-derived components. The transgenic 'Koshihikari' rice, low-glutelin mutant a123, is capable of accumulating large amounts of bioactive peptides in the endosperm. Agrobacterium-mediated transformation using the mutated-acetolactate synthase (mALS) gene expressed under the control of the callus-specific promoter (CSP) as a selectable marker was used to introduce GFP and an anti-hypertensive hexapeptide into 'Koshihikari' a123. The CSP:mALS gene cassette confers pyrimidinyl carboxy herbicide resistance to transgenic rice callus, but is not expressed in regenerated plants. Transformation efficiency of transgenic rice line a123 was improved from about 10% to about 30% by modifying callus induction, callus selection and regeneration media conventionally used for rice tissue culture.</abstract><cop>Berlin</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>17516071</pmid><doi>10.1007/s00299-007-0373-0</doi><tpages>7</tpages></addata></record> |
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| subjects | acetolactate synthase Acetolactate Synthase - genetics Agrobacterium Biological and medical sciences Biotechnology Culture Media Fundamental and applied biological sciences. Psychology Genetic engineering Genetic Markers Genetic technics Genetic Vectors Genome, Plant - genetics Glutens - metabolism Herbicides Koshihikari Methods. Procedures. Technologies Microbiology Mutation - genetics Oryza - genetics Oryza sativa Peptides Plants, Genetically Modified Rhizobium - physiology Rice Transformation, Genetic Transgenic animals and transgenic plants Transgenic plants Transgenic rice |
| title | Agrobacterium-mediated transformation of a low glutelin mutant of 'Koshihikari' rice variety using the mutated-acetolactate synthase gene derived from rice genome as a selectable marker |
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