Development of a droplet digital RT-PCR for the quantification of foot-and-mouth virus RNA

•The droplet-digital PCR was able to quantify FMDV in bovine clinical samples.•The cut-off was determined with accuracy using fifty pre-tested negative samples.•Annealing temperature (AT) was essential to stabilize the total number of droplets.•AT was crucial to differentiate the amplitude of positi...

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Veröffentlicht in:	Journal of virological methods 2018-09, Vol.259, p.129-134
Hauptverfasser:	Pinheiro-de-Oliveira, Tatiana F., Fonseca, Antonio A., Camargos, Marcelo F., Laguardia-Nascimento, Mateus, de Oliveira, Anapolino M., Cottorello, Ana C.P., Goes-Neto, Aristoteles, Barbosa-Stancioli, Edel F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Absolute quantitation Animals Aphthovirus Cattle Cattle Diseases - virology Digital PCR Droplet FMDV identification Foot-and-Mouth Disease - virology Foot-and-Mouth Disease Virus - genetics Foot-and-Mouth Disease Virus - isolation & purification Quantitative PCR Reverse Transcriptase Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - standards RNA, Viral - analysis RNA, Viral - genetics Sensitivity and Specificity Serogroup Viral Load - methods Viral Load - standards
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