Neisseria Meningitidis Detection Based on a Microcalorimetric Biosensor With a Split-Flow Microchannel
This paper proposes and demonstrates a novel microcalorimetric sensor for detecting Neisseria meningitidis. To eliminate additional heating structures and calibration steps, a split-flow microchannel is integrated into the microcalorimeter. The split-flow microchannel constantly maintains the output...
Gespeichert in:
| Veröffentlicht in: | Journal of microelectromechanical systems 2008-06, Vol.17 (3), p.590-598 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 598 |
|---|---|
| container_issue | 3 |
| container_start_page | 590 |
| container_title | Journal of microelectromechanical systems |
| container_volume | 17 |
| creator | YOON, Seung-Il LIM, Mi-Hwa PARK, Se-Chul SHIN, Jeon-Soo KIM, Yong-Jun |
| description | This paper proposes and demonstrates a novel microcalorimetric sensor for detecting Neisseria meningitidis. To eliminate additional heating structures and calibration steps, a split-flow microchannel is integrated into the microcalorimeter. The split-flow microchannel constantly maintains the output of the microcalorimeter near a zero level without the use of any heating elements when there is no biochemical reaction. With the use of the split-flow microchannel, an active heating element such as a heater is no longer required. In addition, to improve the sensitivity of the microcalorimeter, a thermal sensing component, which is a thermopile in this case, has been fabricated on a high thermal resistivity layer, which reduces the parasitic heat transfer to the silicon substrate and concentrates the released thermal energy to the thermopile. The characteristics of the proposed microcalorimeter were investigated by measuring the reaction heat of the biotin-streptavidin pairs. The sensitivity of the microcalorimeter was measured to be 0.21 V/cal. Then, a biological reaction between Neisseria meningitidis group B (NMGB) and its antibody was detected by using the proposed microcalorimeter. In order to verify the reliability of the measurement, exactly the same number of NMGB was reacted with its antibody and an optical density was measured by an enzyme-linked immunosorbent assay as a known reference. |
| doi_str_mv | 10.1109/JMEMS.2008.924846 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_RIE</sourceid><recordid>TN_cdi_proquest_miscellaneous_20619059</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><ieee_id>4538098</ieee_id><sourcerecordid>880648454</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-d93d412eabfdacd47e3aaa90fcfe3152d6da3cb255e66b69705a11ae1a3caefe3</originalsourceid><addsrcrecordid>eNp90U1v1DAQBuAIgURp-QGIS4TExyWLx7Ed-9iWllJ14VAQx2jWmVBXqb3YWaH-e2bZVQ8cevLIfmYkv1NVr0AsAIT7eLk8W14vpBB24aSyyjypDsApaARo-5RrobumA909r16UcisEKGXNQTV-pVAK5YD1kmKIv8IchlDqTzSTn0OK9QkWGmouWASfk8cp5XBHcw6-PgmpUCwp1z_DfMPkej2FuTmf0p-9vsEYaTqqno04FXq5Pw-rH-dn308vmqtvn7-cHl81XoGdm8G1gwJJuBoH9IPqqEVEJ0Y_UgtaDmbA1q-k1mTMyrhOaARAAr5FYnNYvd_NXef0e0Nl7u9C8TRNGCltSm-tMJyOVizfPSqlMOCEdgw_PApBSLmFyjJ98x-9TZsc-cO9A9l2oPQWwQ5xOqVkGvs1x4n5nif12132_3bZb3fZ73bJPW_3g7Fw_mPG6EN5aJRCgTBtx-71zgUienhWurXC2fYv8Qaosg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>912371458</pqid></control><display><type>article</type><title>Neisseria Meningitidis Detection Based on a Microcalorimetric Biosensor With a Split-Flow Microchannel</title><source>IEEE Electronic Library (IEL)</source><creator>YOON, Seung-Il ; LIM, Mi-Hwa ; PARK, Se-Chul ; SHIN, Jeon-Soo ; KIM, Yong-Jun</creator><creatorcontrib>YOON, Seung-Il ; LIM, Mi-Hwa ; PARK, Se-Chul ; SHIN, Jeon-Soo ; KIM, Yong-Jun</creatorcontrib><description>This paper proposes and demonstrates a novel microcalorimetric sensor for detecting Neisseria meningitidis. To eliminate additional heating structures and calibration steps, a split-flow microchannel is integrated into the microcalorimeter. The split-flow microchannel constantly maintains the output of the microcalorimeter near a zero level without the use of any heating elements when there is no biochemical reaction. With the use of the split-flow microchannel, an active heating element such as a heater is no longer required. In addition, to improve the sensitivity of the microcalorimeter, a thermal sensing component, which is a thermopile in this case, has been fabricated on a high thermal resistivity layer, which reduces the parasitic heat transfer to the silicon substrate and concentrates the released thermal energy to the thermopile. The characteristics of the proposed microcalorimeter were investigated by measuring the reaction heat of the biotin-streptavidin pairs. The sensitivity of the microcalorimeter was measured to be 0.21 V/cal. Then, a biological reaction between Neisseria meningitidis group B (NMGB) and its antibody was detected by using the proposed microcalorimeter. In order to verify the reliability of the measurement, exactly the same number of NMGB was reacted with its antibody and an optical density was measured by an enzyme-linked immunosorbent assay as a known reference.</description><identifier>ISSN: 1057-7157</identifier><identifier>EISSN: 1941-0158</identifier><identifier>DOI: 10.1109/JMEMS.2008.924846</identifier><identifier>CODEN: JMIYET</identifier><language>eng</language><publisher>New York, NY: IEEE</publisher><subject>Applied fluid mechanics ; Applied sciences ; Biological and medical sciences ; Biological thermal factors ; biomedical monitoring ; Biomedical optical imaging ; Biosensors ; Biotechnology ; Calibration ; calorimetry ; Conductivity ; Density measurement ; Exact sciences and technology ; Fluid dynamics ; Fluidics ; Fundamental and applied biological sciences. Psychology ; Fundamental areas of phenomenology (including applications) ; Heat transfer ; Heating ; Instruments, apparatus, components and techniques common to several branches of physics and astronomy ; Mechanical engineering. Machine design ; Mechanical instruments, equipment and techniques ; Meningitis ; Methods. Procedures. Technologies ; Microchannel ; Micromechanical devices and systems ; Neisseria meningitidis ; Physics ; Precision engineering, watch making ; sensitivity ; Silicon ; Thermal resistance ; Various methods and equipments</subject><ispartof>Journal of microelectromechanical systems, 2008-06, Vol.17 (3), p.590-598</ispartof><rights>2008 INIST-CNRS</rights><rights>Copyright The Institute of Electrical and Electronics Engineers, Inc. (IEEE) 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-d93d412eabfdacd47e3aaa90fcfe3152d6da3cb255e66b69705a11ae1a3caefe3</citedby><cites>FETCH-LOGICAL-c418t-d93d412eabfdacd47e3aaa90fcfe3152d6da3cb255e66b69705a11ae1a3caefe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/4538098$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>314,780,784,796,27924,27925,54758</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/4538098$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20410637$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>YOON, Seung-Il</creatorcontrib><creatorcontrib>LIM, Mi-Hwa</creatorcontrib><creatorcontrib>PARK, Se-Chul</creatorcontrib><creatorcontrib>SHIN, Jeon-Soo</creatorcontrib><creatorcontrib>KIM, Yong-Jun</creatorcontrib><title>Neisseria Meningitidis Detection Based on a Microcalorimetric Biosensor With a Split-Flow Microchannel</title><title>Journal of microelectromechanical systems</title><addtitle>JMEMS</addtitle><description>This paper proposes and demonstrates a novel microcalorimetric sensor for detecting Neisseria meningitidis. To eliminate additional heating structures and calibration steps, a split-flow microchannel is integrated into the microcalorimeter. The split-flow microchannel constantly maintains the output of the microcalorimeter near a zero level without the use of any heating elements when there is no biochemical reaction. With the use of the split-flow microchannel, an active heating element such as a heater is no longer required. In addition, to improve the sensitivity of the microcalorimeter, a thermal sensing component, which is a thermopile in this case, has been fabricated on a high thermal resistivity layer, which reduces the parasitic heat transfer to the silicon substrate and concentrates the released thermal energy to the thermopile. The characteristics of the proposed microcalorimeter were investigated by measuring the reaction heat of the biotin-streptavidin pairs. The sensitivity of the microcalorimeter was measured to be 0.21 V/cal. Then, a biological reaction between Neisseria meningitidis group B (NMGB) and its antibody was detected by using the proposed microcalorimeter. In order to verify the reliability of the measurement, exactly the same number of NMGB was reacted with its antibody and an optical density was measured by an enzyme-linked immunosorbent assay as a known reference.</description><subject>Applied fluid mechanics</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biological thermal factors</subject><subject>biomedical monitoring</subject><subject>Biomedical optical imaging</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Calibration</subject><subject>calorimetry</subject><subject>Conductivity</subject><subject>Density measurement</subject><subject>Exact sciences and technology</subject><subject>Fluid dynamics</subject><subject>Fluidics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental areas of phenomenology (including applications)</subject><subject>Heat transfer</subject><subject>Heating</subject><subject>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</subject><subject>Mechanical engineering. Machine design</subject><subject>Mechanical instruments, equipment and techniques</subject><subject>Meningitis</subject><subject>Methods. Procedures. Technologies</subject><subject>Microchannel</subject><subject>Micromechanical devices and systems</subject><subject>Neisseria meningitidis</subject><subject>Physics</subject><subject>Precision engineering, watch making</subject><subject>sensitivity</subject><subject>Silicon</subject><subject>Thermal resistance</subject><subject>Various methods and equipments</subject><issn>1057-7157</issn><issn>1941-0158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>RIE</sourceid><recordid>eNp90U1v1DAQBuAIgURp-QGIS4TExyWLx7Ed-9iWllJ14VAQx2jWmVBXqb3YWaH-e2bZVQ8cevLIfmYkv1NVr0AsAIT7eLk8W14vpBB24aSyyjypDsApaARo-5RrobumA909r16UcisEKGXNQTV-pVAK5YD1kmKIv8IchlDqTzSTn0OK9QkWGmouWASfk8cp5XBHcw6-PgmpUCwp1z_DfMPkej2FuTmf0p-9vsEYaTqqno04FXq5Pw-rH-dn308vmqtvn7-cHl81XoGdm8G1gwJJuBoH9IPqqEVEJ0Y_UgtaDmbA1q-k1mTMyrhOaARAAr5FYnNYvd_NXef0e0Nl7u9C8TRNGCltSm-tMJyOVizfPSqlMOCEdgw_PApBSLmFyjJ98x-9TZsc-cO9A9l2oPQWwQ5xOqVkGvs1x4n5nif12132_3bZb3fZ73bJPW_3g7Fw_mPG6EN5aJRCgTBtx-71zgUienhWurXC2fYv8Qaosg</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>YOON, Seung-Il</creator><creator>LIM, Mi-Hwa</creator><creator>PARK, Se-Chul</creator><creator>SHIN, Jeon-Soo</creator><creator>KIM, Yong-Jun</creator><general>IEEE</general><general>Institute of Electrical and Electronics Engineers</general><general>The Institute of Electrical and Electronics Engineers, Inc. (IEEE)</general><scope>97E</scope><scope>RIA</scope><scope>RIE</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope><scope>7QL</scope><scope>7QO</scope><scope>C1K</scope><scope>P64</scope></search><sort><creationdate>20080601</creationdate><title>Neisseria Meningitidis Detection Based on a Microcalorimetric Biosensor With a Split-Flow Microchannel</title><author>YOON, Seung-Il ; LIM, Mi-Hwa ; PARK, Se-Chul ; SHIN, Jeon-Soo ; KIM, Yong-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-d93d412eabfdacd47e3aaa90fcfe3152d6da3cb255e66b69705a11ae1a3caefe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Applied fluid mechanics</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biological thermal factors</topic><topic>biomedical monitoring</topic><topic>Biomedical optical imaging</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Calibration</topic><topic>calorimetry</topic><topic>Conductivity</topic><topic>Density measurement</topic><topic>Exact sciences and technology</topic><topic>Fluid dynamics</topic><topic>Fluidics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental areas of phenomenology (including applications)</topic><topic>Heat transfer</topic><topic>Heating</topic><topic>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</topic><topic>Mechanical engineering. Machine design</topic><topic>Mechanical instruments, equipment and techniques</topic><topic>Meningitis</topic><topic>Methods. Procedures. Technologies</topic><topic>Microchannel</topic><topic>Micromechanical devices and systems</topic><topic>Neisseria meningitidis</topic><topic>Physics</topic><topic>Precision engineering, watch making</topic><topic>sensitivity</topic><topic>Silicon</topic><topic>Thermal resistance</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YOON, Seung-Il</creatorcontrib><creatorcontrib>LIM, Mi-Hwa</creatorcontrib><creatorcontrib>PARK, Se-Chul</creatorcontrib><creatorcontrib>SHIN, Jeon-Soo</creatorcontrib><creatorcontrib>KIM, Yong-Jun</creatorcontrib><collection>IEEE All-Society Periodicals Package (ASPP) 2005-present</collection><collection>IEEE All-Society Periodicals Package (ASPP) 1998-Present</collection><collection>IEEE Electronic Library (IEL)</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of microelectromechanical systems</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YOON, Seung-Il</au><au>LIM, Mi-Hwa</au><au>PARK, Se-Chul</au><au>SHIN, Jeon-Soo</au><au>KIM, Yong-Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neisseria Meningitidis Detection Based on a Microcalorimetric Biosensor With a Split-Flow Microchannel</atitle><jtitle>Journal of microelectromechanical systems</jtitle><stitle>JMEMS</stitle><date>2008-06-01</date><risdate>2008</risdate><volume>17</volume><issue>3</issue><spage>590</spage><epage>598</epage><pages>590-598</pages><issn>1057-7157</issn><eissn>1941-0158</eissn><coden>JMIYET</coden><abstract>This paper proposes and demonstrates a novel microcalorimetric sensor for detecting Neisseria meningitidis. To eliminate additional heating structures and calibration steps, a split-flow microchannel is integrated into the microcalorimeter. The split-flow microchannel constantly maintains the output of the microcalorimeter near a zero level without the use of any heating elements when there is no biochemical reaction. With the use of the split-flow microchannel, an active heating element such as a heater is no longer required. In addition, to improve the sensitivity of the microcalorimeter, a thermal sensing component, which is a thermopile in this case, has been fabricated on a high thermal resistivity layer, which reduces the parasitic heat transfer to the silicon substrate and concentrates the released thermal energy to the thermopile. The characteristics of the proposed microcalorimeter were investigated by measuring the reaction heat of the biotin-streptavidin pairs. The sensitivity of the microcalorimeter was measured to be 0.21 V/cal. Then, a biological reaction between Neisseria meningitidis group B (NMGB) and its antibody was detected by using the proposed microcalorimeter. In order to verify the reliability of the measurement, exactly the same number of NMGB was reacted with its antibody and an optical density was measured by an enzyme-linked immunosorbent assay as a known reference.</abstract><cop>New York, NY</cop><pub>IEEE</pub><doi>10.1109/JMEMS.2008.924846</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | ISSN: 1057-7157 |
| ispartof | Journal of microelectromechanical systems, 2008-06, Vol.17 (3), p.590-598 |
| issn | 1057-7157 1941-0158 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_20619059 |
| source | IEEE Electronic Library (IEL) |
| subjects | Applied fluid mechanics Applied sciences Biological and medical sciences Biological thermal factors biomedical monitoring Biomedical optical imaging Biosensors Biotechnology Calibration calorimetry Conductivity Density measurement Exact sciences and technology Fluid dynamics Fluidics Fundamental and applied biological sciences. Psychology Fundamental areas of phenomenology (including applications) Heat transfer Heating Instruments, apparatus, components and techniques common to several branches of physics and astronomy Mechanical engineering. Machine design Mechanical instruments, equipment and techniques Meningitis Methods. Procedures. Technologies Microchannel Micromechanical devices and systems Neisseria meningitidis Physics Precision engineering, watch making sensitivity Silicon Thermal resistance Various methods and equipments |
| title | Neisseria Meningitidis Detection Based on a Microcalorimetric Biosensor With a Split-Flow Microchannel |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T05%3A48%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_RIE&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Neisseria%20Meningitidis%20Detection%20Based%20on%20a%20Microcalorimetric%20Biosensor%20With%20a%20Split-Flow%20Microchannel&rft.jtitle=Journal%20of%20microelectromechanical%20systems&rft.au=YOON,%20Seung-Il&rft.date=2008-06-01&rft.volume=17&rft.issue=3&rft.spage=590&rft.epage=598&rft.pages=590-598&rft.issn=1057-7157&rft.eissn=1941-0158&rft.coden=JMIYET&rft_id=info:doi/10.1109/JMEMS.2008.924846&rft_dat=%3Cproquest_RIE%3E880648454%3C/proquest_RIE%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=912371458&rft_id=info:pmid/&rft_ieee_id=4538098&rfr_iscdi=true |