Determination of functionally essential neuronal population of the olfactory epithelium for nipple search and subsequent suckling behavior in newborn rats
Abstract Neuronal population of the olfactory epithelium required for nipple search and subsequent suckling behavior was examined in newborn rats. After unilateral ablation of the olfactory bulb, different concentrations of ZnSO4 were contralaterally injected into the nasal cavity to produce varying...
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| Veröffentlicht in: | Brain research 2009-06, Vol.1276, p.50-57 |
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| creator | Kawagishi, Kyutaro Yokouchi, Kumiko Fukushima, Nanae Sakamoto, Michio Sumitomo, Norimi Moriizumi, Tetsuji |
| description | Abstract Neuronal population of the olfactory epithelium required for nipple search and subsequent suckling behavior was examined in newborn rats. After unilateral ablation of the olfactory bulb, different concentrations of ZnSO4 were contralaterally injected into the nasal cavity to produce varying degrees of neuronal degeneration in the olfactory epithelium. The ZnSO4 -treatment resulted in two populations of pups. One exhibited suckling while the other did not, and intact olfactory receptor neurons were quantified immunohistochemically using an antibody for olfactory marker protein (OMP), a marker protein for olfactory receptor neurons. The total numbers of the OMP (+) cells in the ZnSO4 -treated pups with suckling capability ranged between 2457 and 4615, whereas those in the ZnSO4 -treated pups without suckling capability ranged between 112 and 2398. With the mean value (4969) of the total numbers of the OMP (+) cells of the normal/control pups assumed to represent 100%, the total numbers of the OMP (+) cells accounted for 49–93% in the suckling (+) group and 2–48% in the suckling (−) group. From these findings, we conclude that approximately 50% of neuronal population of the olfactory epithelium is a critical value to distinguish between the two groups with and without suckling capability in the unilateral olfactory system of newborn rats. |
| doi_str_mv | 10.1016/j.brainres.2009.04.032 |
| format | Article |
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After unilateral ablation of the olfactory bulb, different concentrations of ZnSO4 were contralaterally injected into the nasal cavity to produce varying degrees of neuronal degeneration in the olfactory epithelium. The ZnSO4 -treatment resulted in two populations of pups. One exhibited suckling while the other did not, and intact olfactory receptor neurons were quantified immunohistochemically using an antibody for olfactory marker protein (OMP), a marker protein for olfactory receptor neurons. The total numbers of the OMP (+) cells in the ZnSO4 -treated pups with suckling capability ranged between 2457 and 4615, whereas those in the ZnSO4 -treated pups without suckling capability ranged between 112 and 2398. With the mean value (4969) of the total numbers of the OMP (+) cells of the normal/control pups assumed to represent 100%, the total numbers of the OMP (+) cells accounted for 49–93% in the suckling (+) group and 2–48% in the suckling (−) group. From these findings, we conclude that approximately 50% of neuronal population of the olfactory epithelium is a critical value to distinguish between the two groups with and without suckling capability in the unilateral olfactory system of newborn rats.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2009.04.032</identifier><identifier>PMID: 19393636</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis of Variance ; Animals ; Animals, Newborn ; Appetitive Behavior - physiology ; Biological and medical sciences ; Cell Count ; Feeding Behavior - physiology ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Lactation - physiology ; Nerve Degeneration - chemically induced ; Neurology ; Neurons - drug effects ; Neurons - physiology ; Neurotoxins - toxicity ; Newborn rat ; Nipple attachment ; Nipple search ; Olfactory Bulb - injuries ; Olfactory marker protein ; Olfactory Marker Protein - metabolism ; Olfactory Mucosa - drug effects ; Olfactory Mucosa - innervation ; Olfactory Mucosa - physiology ; Olfactory Receptor Neurons - drug effects ; Olfactory Receptor Neurons - physiology ; Olfactory system and olfaction. Gustatory system and gustation ; Rats ; Rats, Wistar ; Vertebrates: nervous system and sense organs ; Zinc Sulfate - toxicity</subject><ispartof>Brain research, 2009-06, Vol.1276, p.50-57</ispartof><rights>Elsevier B.V.</rights><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c548t-37894a5b79575982471a2e225eb2a3355887bb0bea7f15b8f40c0c4b73f6821b3</citedby><cites>FETCH-LOGICAL-c548t-37894a5b79575982471a2e225eb2a3355887bb0bea7f15b8f40c0c4b73f6821b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.brainres.2009.04.032$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21640570$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19393636$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawagishi, Kyutaro</creatorcontrib><creatorcontrib>Yokouchi, Kumiko</creatorcontrib><creatorcontrib>Fukushima, Nanae</creatorcontrib><creatorcontrib>Sakamoto, Michio</creatorcontrib><creatorcontrib>Sumitomo, Norimi</creatorcontrib><creatorcontrib>Moriizumi, Tetsuji</creatorcontrib><title>Determination of functionally essential neuronal population of the olfactory epithelium for nipple search and subsequent suckling behavior in newborn rats</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Abstract Neuronal population of the olfactory epithelium required for nipple search and subsequent suckling behavior was examined in newborn rats. After unilateral ablation of the olfactory bulb, different concentrations of ZnSO4 were contralaterally injected into the nasal cavity to produce varying degrees of neuronal degeneration in the olfactory epithelium. The ZnSO4 -treatment resulted in two populations of pups. One exhibited suckling while the other did not, and intact olfactory receptor neurons were quantified immunohistochemically using an antibody for olfactory marker protein (OMP), a marker protein for olfactory receptor neurons. The total numbers of the OMP (+) cells in the ZnSO4 -treated pups with suckling capability ranged between 2457 and 4615, whereas those in the ZnSO4 -treated pups without suckling capability ranged between 112 and 2398. With the mean value (4969) of the total numbers of the OMP (+) cells of the normal/control pups assumed to represent 100%, the total numbers of the OMP (+) cells accounted for 49–93% in the suckling (+) group and 2–48% in the suckling (−) group. From these findings, we conclude that approximately 50% of neuronal population of the olfactory epithelium is a critical value to distinguish between the two groups with and without suckling capability in the unilateral olfactory system of newborn rats.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Appetitive Behavior - physiology</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Feeding Behavior - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Lactation - physiology</subject><subject>Nerve Degeneration - chemically induced</subject><subject>Neurology</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>Neurotoxins - toxicity</subject><subject>Newborn rat</subject><subject>Nipple attachment</subject><subject>Nipple search</subject><subject>Olfactory Bulb - injuries</subject><subject>Olfactory marker protein</subject><subject>Olfactory Marker Protein - metabolism</subject><subject>Olfactory Mucosa - drug effects</subject><subject>Olfactory Mucosa - innervation</subject><subject>Olfactory Mucosa - physiology</subject><subject>Olfactory Receptor Neurons - drug effects</subject><subject>Olfactory Receptor Neurons - physiology</subject><subject>Olfactory system and olfaction. Gustatory system and gustation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Zinc Sulfate - toxicity</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks2OFCEQgDtG446rr7DhorcZC-hu4GI062-yiQf1TICpdphloIXuNfMqPq10ZlwTL54oKl_9hI-muaKwoUD7l_uNzcbHjGXDANQG2g1w9qBZUSnYumctPGxWANCvpVL8onlSyr5eOVfwuLmgiive837V_HqLE-aDj2byKZI0kGGObolNCEeCpWCcvAkk4pyXJBnTOId7etohSWEwbkq54qOvieDnAxlSJtGPY0BS0GS3IyZuSZltwR9z7VlDdxt8_E4s7sydr7iPdcpPm3Ik2UzlafNoMKHgs_N52Xx7_-7r9cf1zecPn67f3Kxd18ppzYVUremsUJ3olGStoIYhYx1aZjjvOimFtWDRiIF2Vg4tOHCtFXzoJaOWXzYvTn3HnOpqZdIHXxyGYCKmuWgGPZWdEhXsT6DLqZSMgx6zP5h81BT0YkXv9R8rerGiodXVSi28Ok-Y7QG3f8vOGirw_AyY4kwYsonOl3uO0b6FTkDlXp84rO9x5zHr4jxGh1uf0U16m_z_d3n1TwtXLfg69RaPWPZpztVy0VQXpkF_Wf7Q8oVAAQhJKf8NlKDHlw</recordid><startdate>20090618</startdate><enddate>20090618</enddate><creator>Kawagishi, Kyutaro</creator><creator>Yokouchi, Kumiko</creator><creator>Fukushima, Nanae</creator><creator>Sakamoto, Michio</creator><creator>Sumitomo, Norimi</creator><creator>Moriizumi, Tetsuji</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20090618</creationdate><title>Determination of functionally essential neuronal population of the olfactory epithelium for nipple search and subsequent suckling behavior in newborn rats</title><author>Kawagishi, Kyutaro ; Yokouchi, Kumiko ; Fukushima, Nanae ; Sakamoto, Michio ; Sumitomo, Norimi ; Moriizumi, Tetsuji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c548t-37894a5b79575982471a2e225eb2a3355887bb0bea7f15b8f40c0c4b73f6821b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Appetitive Behavior - physiology</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Feeding Behavior - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Lactation - physiology</topic><topic>Nerve Degeneration - chemically induced</topic><topic>Neurology</topic><topic>Neurons - drug effects</topic><topic>Neurons - physiology</topic><topic>Neurotoxins - toxicity</topic><topic>Newborn rat</topic><topic>Nipple attachment</topic><topic>Nipple search</topic><topic>Olfactory Bulb - injuries</topic><topic>Olfactory marker protein</topic><topic>Olfactory Marker Protein - metabolism</topic><topic>Olfactory Mucosa - drug effects</topic><topic>Olfactory Mucosa - innervation</topic><topic>Olfactory Mucosa - physiology</topic><topic>Olfactory Receptor Neurons - drug effects</topic><topic>Olfactory Receptor Neurons - physiology</topic><topic>Olfactory system and olfaction. Gustatory system and gustation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Zinc Sulfate - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawagishi, Kyutaro</creatorcontrib><creatorcontrib>Yokouchi, Kumiko</creatorcontrib><creatorcontrib>Fukushima, Nanae</creatorcontrib><creatorcontrib>Sakamoto, Michio</creatorcontrib><creatorcontrib>Sumitomo, Norimi</creatorcontrib><creatorcontrib>Moriizumi, Tetsuji</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawagishi, Kyutaro</au><au>Yokouchi, Kumiko</au><au>Fukushima, Nanae</au><au>Sakamoto, Michio</au><au>Sumitomo, Norimi</au><au>Moriizumi, Tetsuji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of functionally essential neuronal population of the olfactory epithelium for nipple search and subsequent suckling behavior in newborn rats</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2009-06-18</date><risdate>2009</risdate><volume>1276</volume><spage>50</spage><epage>57</epage><pages>50-57</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Abstract Neuronal population of the olfactory epithelium required for nipple search and subsequent suckling behavior was examined in newborn rats. After unilateral ablation of the olfactory bulb, different concentrations of ZnSO4 were contralaterally injected into the nasal cavity to produce varying degrees of neuronal degeneration in the olfactory epithelium. The ZnSO4 -treatment resulted in two populations of pups. One exhibited suckling while the other did not, and intact olfactory receptor neurons were quantified immunohistochemically using an antibody for olfactory marker protein (OMP), a marker protein for olfactory receptor neurons. The total numbers of the OMP (+) cells in the ZnSO4 -treated pups with suckling capability ranged between 2457 and 4615, whereas those in the ZnSO4 -treated pups without suckling capability ranged between 112 and 2398. With the mean value (4969) of the total numbers of the OMP (+) cells of the normal/control pups assumed to represent 100%, the total numbers of the OMP (+) cells accounted for 49–93% in the suckling (+) group and 2–48% in the suckling (−) group. From these findings, we conclude that approximately 50% of neuronal population of the olfactory epithelium is a critical value to distinguish between the two groups with and without suckling capability in the unilateral olfactory system of newborn rats.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19393636</pmid><doi>10.1016/j.brainres.2009.04.032</doi><tpages>8</tpages></addata></record> |
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| subjects | Analysis of Variance Animals Animals, Newborn Appetitive Behavior - physiology Biological and medical sciences Cell Count Feeding Behavior - physiology Fundamental and applied biological sciences. Psychology Immunohistochemistry Lactation - physiology Nerve Degeneration - chemically induced Neurology Neurons - drug effects Neurons - physiology Neurotoxins - toxicity Newborn rat Nipple attachment Nipple search Olfactory Bulb - injuries Olfactory marker protein Olfactory Marker Protein - metabolism Olfactory Mucosa - drug effects Olfactory Mucosa - innervation Olfactory Mucosa - physiology Olfactory Receptor Neurons - drug effects Olfactory Receptor Neurons - physiology Olfactory system and olfaction. Gustatory system and gustation Rats Rats, Wistar Vertebrates: nervous system and sense organs Zinc Sulfate - toxicity |
| title | Determination of functionally essential neuronal population of the olfactory epithelium for nipple search and subsequent suckling behavior in newborn rats |
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