Quantitative measurement of fathead minnow vitellogenin mRNA using hybridization protection assays

We have developed a novel test system for the quantitative assessment of gene transcription. The procedure involves the use of chemiluminescent‐labeled oligonucleotide probes in a hybridization protection assay (HPA) format. We have used this technology to measure changes in vitellogenin mRNA to dem...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Environmental toxicology and chemistry 2003-05, Vol.22 (5), p.992-995
Hauptverfasser:	Thomas-Jones, Emma, Walkley, Neal, Morris, Ceri, Kille, Peter, Cryer, Jennifer, Weeks, Ian, Woodhead, J. Stuart
Format:	Artikel
Sprache:	eng
Schlagworte:	Agnatha. Pisces Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences Cyprinidae - genetics Cyprinidae - metabolism Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on vertebrates Endocrine disruption Environmental Exposure - analysis Environmental Monitoring - methods Estradiol - pharmacology Estradiol - toxicity estrogenic activity Fathead minnow Freshwater Fundamental and applied biological sciences. Psychology Gene expression Hybridization protection assay Injections, Intraperitoneal Luminescent Measurements Nucleic Acid Hybridization - methods Oligonucleotide Probes - analysis Oligonucleotide Probes - chemistry Pimephales promelas Reproducibility of Results RNA, Messenger - analysis RNA, Messenger - genetics Techniques Transcription, Genetic - drug effects Vitellogenin Vitellogenins - biosynthesis Vitellogenins - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!