Detection of tobamoviruses by RT-PCR using a novel pair of degenerate primers

•A generic RT-PCR assay for the universal detection of tobamoviruses using a novel pair of degenerate primers TobamodF/TobamodR was established.•The sensitivity of the RT-PCR assay was examined, and the detection limits of the assay were at least 10−7 for ToMMV, 10−5 for ORSV and 10−6 for CGMMV.•The...

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Veröffentlicht in:	Journal of virological methods 2018-09, Vol.259, p.122-128
Hauptverfasser:	Li, Yueyue, Tan, Guanlin, Lan, Pingxiu, Zhang, Ansheng, Liu, Yong, Li, Ruhui, Li, Fan
Format:	Artikel
Sprache:	eng
Schlagworte:	Capsicum - virology China Cost-Benefit Analysis DNA Primers - genetics Generic RT-PCR assay Lycopersicon esculentum - virology Molecular Diagnostic Techniques - methods Plant Diseases - virology Reverse Transcriptase Polymerase Chain Reaction - methods Sensitivity and Specificity Tobamovirus Tobamovirus - genetics Tobamovirus - isolation & purification Universal primers Virus detection
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