Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger
Human promonocytic cell line U937 cells can be induced to differentiate into macrophages by treatment with 12- O -tetradecanoylphorbol-13-acetate (TPA). TPA treatment induced the expression of the monocytic differentiation markers CD11b and CD36, with concomitant morphological changes. Moreover, TPA...
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| Veröffentlicht in: | Leukemia 2009-04, Vol.23 (4), p.761-769 |
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| creator | Yamamoto, T Sakaguchi, N Hachiya, M Nakayama, F Yamakawa, M Akashi, M |
| description | Human promonocytic cell line U937 cells can be induced to differentiate into macrophages by treatment with 12-
O
-tetradecanoylphorbol-13-acetate (TPA). TPA treatment induced the expression of the monocytic differentiation markers CD11b and CD36, with concomitant morphological changes. Moreover, TPA enhanced reactive oxygen species (ROS) generation in these cells, and phagocytic ability was also stimulated during differentiation. The antioxidant agent
N
-acetyl-
L
-cysteine inhibited the TPA-induced differentiation of U937 cells. TPA treatment decreased the expression level of catalase, which catalyzes the decomposition of hydrogen peroxide (H
2
O
2
) to H
2
O and O
2
. In contrast, TPA increased the level of manganese superoxide dismutase, which catalyzes the dismutation of superoxide into H
2
O
2
and O
2
without affecting the levels of copper-zinc superoxide dismutase or glutathione peroxidase 1, which removes H
2
O
2
using glutathione as substrate. Treatment of U937 cells with catalase inhibited the enhancement of ROS generation induced by TPA, and blocked the TPA-induced differentiation of U937 cells. Human promyelocytic cell line HL60 cells were also induced to differentiate into macrophages by TPA. However, HP100-1 cells, its variant cell line overexpressing catalase, were resistant to TPA-induced differentiation. Our results suggest that catalase inhibits monocytic differentiation by TPA; the decrease in catalase level and the accumulation of H
2
O
2
are significant events for monocyte/macrophage differentiation by TPA. |
| doi_str_mv | 10.1038/leu.2008.353 |
| format | Article |
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O
-tetradecanoylphorbol-13-acetate (TPA). TPA treatment induced the expression of the monocytic differentiation markers CD11b and CD36, with concomitant morphological changes. Moreover, TPA enhanced reactive oxygen species (ROS) generation in these cells, and phagocytic ability was also stimulated during differentiation. The antioxidant agent
N
-acetyl-
L
-cysteine inhibited the TPA-induced differentiation of U937 cells. TPA treatment decreased the expression level of catalase, which catalyzes the decomposition of hydrogen peroxide (H
2
O
2
) to H
2
O and O
2
. In contrast, TPA increased the level of manganese superoxide dismutase, which catalyzes the dismutation of superoxide into H
2
O
2
and O
2
without affecting the levels of copper-zinc superoxide dismutase or glutathione peroxidase 1, which removes H
2
O
2
using glutathione as substrate. Treatment of U937 cells with catalase inhibited the enhancement of ROS generation induced by TPA, and blocked the TPA-induced differentiation of U937 cells. Human promyelocytic cell line HL60 cells were also induced to differentiate into macrophages by TPA. However, HP100-1 cells, its variant cell line overexpressing catalase, were resistant to TPA-induced differentiation. Our results suggest that catalase inhibits monocytic differentiation by TPA; the decrease in catalase level and the accumulation of H
2
O
2
are significant events for monocyte/macrophage differentiation by TPA.</description><identifier>ISSN: 0887-6924</identifier><identifier>EISSN: 1476-5551</identifier><identifier>DOI: 10.1038/leu.2008.353</identifier><identifier>PMID: 19092850</identifier><identifier>CODEN: LEUKED</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>12-O-Tetradecanoylphorbol-13-acetate ; Acetic acid ; Acetylcysteine ; Antioxidants ; Biological and medical sciences ; Cancer Research ; Catalase ; Catalase - analysis ; Catalase - physiology ; CD11b antigen ; CD36 antigen ; Cell differentiation ; Cell Differentiation - drug effects ; Cell growth ; Critical Care Medicine ; Differentiation ; Emergency medical care ; Glutathione ; Glutathione peroxidase ; Hematologic and hematopoietic diseases ; Hematology ; Humans ; Hydrogen Peroxide ; Intensive ; Internal Medicine ; Leukemia ; Macrophages ; Macrophages - cytology ; Manganese ; Medical research ; Medical sciences ; Medicine ; Medicine & Public Health ; Monocytes ; Monocytes - cytology ; Oncology ; original-article ; Oxidation ; Peroxidase ; Phagocytes ; Phorbol esters ; Physiological aspects ; Polyclonal antibodies ; Radiation ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Roles ; Second Messenger Systems ; Stem cells ; Superoxide dismutase ; Superoxide Dismutase - analysis ; Tetradecanoylphorbol Acetate - pharmacology ; U937 Cells</subject><ispartof>Leukemia, 2009-04, Vol.23 (4), p.761-769</ispartof><rights>Macmillan Publishers Limited 2009</rights><rights>2009 INIST-CNRS</rights><rights>COPYRIGHT 2009 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Apr 2009</rights><rights>Macmillan Publishers Limited 2009.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c651t-269b2ce5d3636ca18df682a0130cbbb6d72848a3871f4623f080c5e8d6b660493</citedby><cites>FETCH-LOGICAL-c651t-269b2ce5d3636ca18df682a0130cbbb6d72848a3871f4623f080c5e8d6b660493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/leu.2008.353$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/leu.2008.353$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21431643$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19092850$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamamoto, T</creatorcontrib><creatorcontrib>Sakaguchi, N</creatorcontrib><creatorcontrib>Hachiya, M</creatorcontrib><creatorcontrib>Nakayama, F</creatorcontrib><creatorcontrib>Yamakawa, M</creatorcontrib><creatorcontrib>Akashi, M</creatorcontrib><title>Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger</title><title>Leukemia</title><addtitle>Leukemia</addtitle><addtitle>Leukemia</addtitle><description>Human promonocytic cell line U937 cells can be induced to differentiate into macrophages by treatment with 12-
O
-tetradecanoylphorbol-13-acetate (TPA). TPA treatment induced the expression of the monocytic differentiation markers CD11b and CD36, with concomitant morphological changes. Moreover, TPA enhanced reactive oxygen species (ROS) generation in these cells, and phagocytic ability was also stimulated during differentiation. The antioxidant agent
N
-acetyl-
L
-cysteine inhibited the TPA-induced differentiation of U937 cells. TPA treatment decreased the expression level of catalase, which catalyzes the decomposition of hydrogen peroxide (H
2
O
2
) to H
2
O and O
2
. In contrast, TPA increased the level of manganese superoxide dismutase, which catalyzes the dismutation of superoxide into H
2
O
2
and O
2
without affecting the levels of copper-zinc superoxide dismutase or glutathione peroxidase 1, which removes H
2
O
2
using glutathione as substrate. Treatment of U937 cells with catalase inhibited the enhancement of ROS generation induced by TPA, and blocked the TPA-induced differentiation of U937 cells. Human promyelocytic cell line HL60 cells were also induced to differentiate into macrophages by TPA. However, HP100-1 cells, its variant cell line overexpressing catalase, were resistant to TPA-induced differentiation. Our results suggest that catalase inhibits monocytic differentiation by TPA; the decrease in catalase level and the accumulation of H
2
O
2
are significant events for monocyte/macrophage differentiation by TPA.</description><subject>12-O-Tetradecanoylphorbol-13-acetate</subject><subject>Acetic acid</subject><subject>Acetylcysteine</subject><subject>Antioxidants</subject><subject>Biological and medical sciences</subject><subject>Cancer Research</subject><subject>Catalase</subject><subject>Catalase - analysis</subject><subject>Catalase - physiology</subject><subject>CD11b antigen</subject><subject>CD36 antigen</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell growth</subject><subject>Critical Care Medicine</subject><subject>Differentiation</subject><subject>Emergency medical care</subject><subject>Glutathione</subject><subject>Glutathione peroxidase</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Hematology</subject><subject>Humans</subject><subject>Hydrogen Peroxide</subject><subject>Intensive</subject><subject>Internal Medicine</subject><subject>Leukemia</subject><subject>Macrophages</subject><subject>Macrophages - cytology</subject><subject>Manganese</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Monocytes</subject><subject>Monocytes - cytology</subject><subject>Oncology</subject><subject>original-article</subject><subject>Oxidation</subject><subject>Peroxidase</subject><subject>Phagocytes</subject><subject>Phorbol esters</subject><subject>Physiological aspects</subject><subject>Polyclonal antibodies</subject><subject>Radiation</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Roles</subject><subject>Second Messenger Systems</subject><subject>Stem cells</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - analysis</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>U937 Cells</subject><issn>0887-6924</issn><issn>1476-5551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kk1v1DAQhiMEoqVw44wsED2Rxd9xuK0qvqRKINSeLccZ77ok9mInEvvvcbQr2qIiHyx5nnlnPPNW1UuCVwQz9X6AeUUxVism2KPqlPBG1kII8rg6xUo1tWwpP6me5XyD8RKUT6sT0uKWKoFPq58_4gAoOmTNZAaTAfmAxhii3U_eot47BwnC5M3kY1jA65Y1yMIwZNTt0dX39Qe03fcpbiCgHaT42_eATEYGZbAx9GiEnCFsID2vnjgzZHhxvM-q608fry6-1JffPn-9WF_WVgoy1VS2HbUgeiaZtIao3klFDSYM267rZN9QxZVhqiGOS8ocVtgKUL3spMS8ZWfV-UF3l-KvGfKkR5-Xjk2AOGdNscSylaqAb_4Bb-KcQulNU8lFw7hsSKFe_5eiWHBF1R2pjRlA--DilIxd6uo1aRUnosG8UKsHqHJ6GH0ZFjhf3u8lnN9J2IIZpm2Ow7wsI98H3x1Am2LOCZzeJT-atNcE68UnuvhELz7RxScFf3X809yN0N_CR2MU4O0RMNmawSUTrM9_OUo4I5IvQvWByyW0LPl2OA8W_gOxNs-R</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Yamamoto, T</creator><creator>Sakaguchi, N</creator><creator>Hachiya, M</creator><creator>Nakayama, F</creator><creator>Yamakawa, M</creator><creator>Akashi, M</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20090401</creationdate><title>Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger</title><author>Yamamoto, T ; Sakaguchi, N ; Hachiya, M ; Nakayama, F ; Yamakawa, M ; Akashi, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c651t-269b2ce5d3636ca18df682a0130cbbb6d72848a3871f4623f080c5e8d6b660493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>12-O-Tetradecanoylphorbol-13-acetate</topic><topic>Acetic acid</topic><topic>Acetylcysteine</topic><topic>Antioxidants</topic><topic>Biological and medical sciences</topic><topic>Cancer Research</topic><topic>Catalase</topic><topic>Catalase - analysis</topic><topic>Catalase - physiology</topic><topic>CD11b antigen</topic><topic>CD36 antigen</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell growth</topic><topic>Critical Care Medicine</topic><topic>Differentiation</topic><topic>Emergency medical care</topic><topic>Glutathione</topic><topic>Glutathione peroxidase</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Hematology</topic><topic>Humans</topic><topic>Hydrogen Peroxide</topic><topic>Intensive</topic><topic>Internal Medicine</topic><topic>Leukemia</topic><topic>Macrophages</topic><topic>Macrophages - cytology</topic><topic>Manganese</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Monocytes</topic><topic>Monocytes - cytology</topic><topic>Oncology</topic><topic>original-article</topic><topic>Oxidation</topic><topic>Peroxidase</topic><topic>Phagocytes</topic><topic>Phorbol esters</topic><topic>Physiological aspects</topic><topic>Polyclonal antibodies</topic><topic>Radiation</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Roles</topic><topic>Second Messenger Systems</topic><topic>Stem cells</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - analysis</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>U937 Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamamoto, T</creatorcontrib><creatorcontrib>Sakaguchi, N</creatorcontrib><creatorcontrib>Hachiya, M</creatorcontrib><creatorcontrib>Nakayama, F</creatorcontrib><creatorcontrib>Yamakawa, M</creatorcontrib><creatorcontrib>Akashi, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database (ProQuest)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Leukemia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamamoto, T</au><au>Sakaguchi, N</au><au>Hachiya, M</au><au>Nakayama, F</au><au>Yamakawa, M</au><au>Akashi, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger</atitle><jtitle>Leukemia</jtitle><stitle>Leukemia</stitle><addtitle>Leukemia</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>23</volume><issue>4</issue><spage>761</spage><epage>769</epage><pages>761-769</pages><issn>0887-6924</issn><eissn>1476-5551</eissn><coden>LEUKED</coden><abstract>Human promonocytic cell line U937 cells can be induced to differentiate into macrophages by treatment with 12-
O
-tetradecanoylphorbol-13-acetate (TPA). TPA treatment induced the expression of the monocytic differentiation markers CD11b and CD36, with concomitant morphological changes. Moreover, TPA enhanced reactive oxygen species (ROS) generation in these cells, and phagocytic ability was also stimulated during differentiation. The antioxidant agent
N
-acetyl-
L
-cysteine inhibited the TPA-induced differentiation of U937 cells. TPA treatment decreased the expression level of catalase, which catalyzes the decomposition of hydrogen peroxide (H
2
O
2
) to H
2
O and O
2
. In contrast, TPA increased the level of manganese superoxide dismutase, which catalyzes the dismutation of superoxide into H
2
O
2
and O
2
without affecting the levels of copper-zinc superoxide dismutase or glutathione peroxidase 1, which removes H
2
O
2
using glutathione as substrate. Treatment of U937 cells with catalase inhibited the enhancement of ROS generation induced by TPA, and blocked the TPA-induced differentiation of U937 cells. Human promyelocytic cell line HL60 cells were also induced to differentiate into macrophages by TPA. However, HP100-1 cells, its variant cell line overexpressing catalase, were resistant to TPA-induced differentiation. Our results suggest that catalase inhibits monocytic differentiation by TPA; the decrease in catalase level and the accumulation of H
2
O
2
are significant events for monocyte/macrophage differentiation by TPA.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>19092850</pmid><doi>10.1038/leu.2008.353</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Leukemia, 2009-04, Vol.23 (4), p.761-769 |
| issn | 0887-6924 1476-5551 |
| language | eng |
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| source | MEDLINE; Nature; SpringerLink (Online service); EZB Electronic Journals Library |
| subjects | 12-O-Tetradecanoylphorbol-13-acetate Acetic acid Acetylcysteine Antioxidants Biological and medical sciences Cancer Research Catalase Catalase - analysis Catalase - physiology CD11b antigen CD36 antigen Cell differentiation Cell Differentiation - drug effects Cell growth Critical Care Medicine Differentiation Emergency medical care Glutathione Glutathione peroxidase Hematologic and hematopoietic diseases Hematology Humans Hydrogen Peroxide Intensive Internal Medicine Leukemia Macrophages Macrophages - cytology Manganese Medical research Medical sciences Medicine Medicine & Public Health Monocytes Monocytes - cytology Oncology original-article Oxidation Peroxidase Phagocytes Phorbol esters Physiological aspects Polyclonal antibodies Radiation Reactive oxygen species Reactive Oxygen Species - metabolism Roles Second Messenger Systems Stem cells Superoxide dismutase Superoxide Dismutase - analysis Tetradecanoylphorbol Acetate - pharmacology U937 Cells |
| title | Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger |
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