Unfolding of the immunoglobulin light and heavy chains is required for the enzymatic removal of N-terminal pyroglutamyl residues

To enable Edman sequencing of pyroglutamylated immunoglobulins, enzymatic deblocking by pyroglutamate aminopeptidase is performed, often with variable yield and compromised solubility. Recently, enzymatic deblocking of immunoglobulins without denaturation was described. Although the conditions ensur...

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Veröffentlicht in:	Biochimica et biophysica acta 2006-11, Vol.1764 (11), p.1735-1740
Hauptverfasser:	Hellström, Johanna L., Vehniäinen, Markus, Mustonen, Merja, Lövgren, Timo, Lamminmäki, Urpo, Hellman, Jukka
Format:	Artikel
Sprache:	eng
Schlagworte:	Aggregation Deblocking Electrophoresis, Polyacrylamide Gel Hot Temperature Immunoglobulin Immunoglobulin Heavy Chains - metabolism Immunoglobulin Light Chains - metabolism Models, Molecular Protein Denaturation Protein sequencing Pyrococcus furiosus - enzymology Pyroglutamate aminopeptidase Pyroglutamyl-Peptidase I - metabolism Pyrrolidonecarboxylic Acid - metabolism Unfolding
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