Effects of tumour necrosis factor‐alpha and interleukin‐1 beta on in vitro development of bovine secondary follicles
The objective of this study was to determine the effects of TNF‐α and IL‐1β on development and survival of bovine secondary follicle culture in vitro for 18 days. Secondary follicles (~0.2 mm) were isolated from ovarian cortex and individually cultured at 38.5°C, with 5% CO2 in air, for 18 days, in...
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| Veröffentlicht in: | Reproduction in domestic animals 2018-08, Vol.53 (4), p.997-1005 |
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| creator | Paulino, Lais R. F. M. Cunha, Ellen V. Barbalho Silva, Anderson W. Souza, Glaucinete B. Lopes, Ewerton P. F. Donato, Mariana A. M. Peixoto, Cristina A. Matos‐Brito, Bruno G. Hurk, Robert Silva, José Roberto V. |
| description | The objective of this study was to determine the effects of TNF‐α and IL‐1β on development and survival of bovine secondary follicle culture in vitro for 18 days. Secondary follicles (~0.2 mm) were isolated from ovarian cortex and individually cultured at 38.5°C, with 5% CO2 in air, for 18 days, in TCM‐199+ alone (cultured control) or supplemented with 10 ng/ml IL‐1β, 10 ng/ml TNF‐α or both TNF‐α and IL‐1β. The effects of these treatments on growth, follicular survival, antrum formation, viability, ultrastructure and mRNA levels for GDF‐9, c‐MOS, H1foo and Cyclin B1 were evaluated. The results showed that addition of TNF‐α to culture medium increased follicular diameter and rate of antrum formation, whereas that of IL‐1β and a mixture of IL‐1β and TNF‐α did not do so. Ultrastructural analysis showed that, among the tested cytokine treatments, follicles cultured in the presence of TNF‐α had the best‐preserved oocytes and granulosa cells. The presence of TNF‐α, IL‐1β or both did not influence the expression of mRNAs analysed. In conclusion, in contrast to IL‐1β, TNF‐α promotes growth of and antrum formation in in vitro cultured bovine secondary follicles, while their ultrastructure and viability were maintained. |
| doi_str_mv | 10.1111/rda.13199 |
| format | Article |
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F. M. ; Cunha, Ellen V. ; Barbalho Silva, Anderson W. ; Souza, Glaucinete B. ; Lopes, Ewerton P. F. ; Donato, Mariana A. M. ; Peixoto, Cristina A. ; Matos‐Brito, Bruno G. ; Hurk, Robert ; Silva, José Roberto V.</creator><creatorcontrib>Paulino, Lais R. F. M. ; Cunha, Ellen V. ; Barbalho Silva, Anderson W. ; Souza, Glaucinete B. ; Lopes, Ewerton P. F. ; Donato, Mariana A. M. ; Peixoto, Cristina A. ; Matos‐Brito, Bruno G. ; Hurk, Robert ; Silva, José Roberto V.</creatorcontrib><description>The objective of this study was to determine the effects of TNF‐α and IL‐1β on development and survival of bovine secondary follicle culture in vitro for 18 days. Secondary follicles (~0.2 mm) were isolated from ovarian cortex and individually cultured at 38.5°C, with 5% CO2 in air, for 18 days, in TCM‐199+ alone (cultured control) or supplemented with 10 ng/ml IL‐1β, 10 ng/ml TNF‐α or both TNF‐α and IL‐1β. The effects of these treatments on growth, follicular survival, antrum formation, viability, ultrastructure and mRNA levels for GDF‐9, c‐MOS, H1foo and Cyclin B1 were evaluated. The results showed that addition of TNF‐α to culture medium increased follicular diameter and rate of antrum formation, whereas that of IL‐1β and a mixture of IL‐1β and TNF‐α did not do so. Ultrastructural analysis showed that, among the tested cytokine treatments, follicles cultured in the presence of TNF‐α had the best‐preserved oocytes and granulosa cells. The presence of TNF‐α, IL‐1β or both did not influence the expression of mRNAs analysed. In conclusion, in contrast to IL‐1β, TNF‐α promotes growth of and antrum formation in in vitro cultured bovine secondary follicles, while their ultrastructure and viability were maintained.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.13199</identifier><identifier>PMID: 29943395</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animals ; Carbon dioxide ; Cattle - physiology ; Cell culture ; cow ; culture ; Cyclin B1 ; Cyclin B1 - genetics ; Cyclin B1 - metabolism ; Female ; Follicles ; gene expression ; Gene Expression Regulation - drug effects ; Granulosa cells ; Growth Differentiation Factor 9 - genetics ; Growth Differentiation Factor 9 - metabolism ; Histones - genetics ; Histones - metabolism ; IL-1β ; Interleukin-1beta - administration & dosage ; Interleukin-1beta - pharmacology ; Interleukins ; mRNA ; Oocytes ; Ovarian Follicle - drug effects ; Ovarian Follicle - growth & development ; ovarian follicles ; Proto-Oncogene Proteins c-mos - genetics ; Proto-Oncogene Proteins c-mos - metabolism ; Survival ; Tissue Culture Techniques - veterinary ; Tumor necrosis factor ; Tumor Necrosis Factor-alpha - administration & dosage ; Tumor Necrosis Factor-alpha - pharmacology ; Tumor necrosis factor-TNF ; Tumors ; Ultrastructure ; Viability</subject><ispartof>Reproduction in domestic animals, 2018-08, Vol.53 (4), p.997-1005</ispartof><rights>2018 Blackwell Verlag GmbH</rights><rights>2018 Blackwell Verlag GmbH.</rights><rights>Copyright © 2018 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-33ae848377547980c3e9bbcd140ee62a29a7028fdddb7f840bb00e2205fc0bd03</citedby><cites>FETCH-LOGICAL-c3539-33ae848377547980c3e9bbcd140ee62a29a7028fdddb7f840bb00e2205fc0bd03</cites><orcidid>0000-0002-5970-6177</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.13199$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.13199$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29943395$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paulino, Lais R. F. M.</creatorcontrib><creatorcontrib>Cunha, Ellen V.</creatorcontrib><creatorcontrib>Barbalho Silva, Anderson W.</creatorcontrib><creatorcontrib>Souza, Glaucinete B.</creatorcontrib><creatorcontrib>Lopes, Ewerton P. F.</creatorcontrib><creatorcontrib>Donato, Mariana A. M.</creatorcontrib><creatorcontrib>Peixoto, Cristina A.</creatorcontrib><creatorcontrib>Matos‐Brito, Bruno G.</creatorcontrib><creatorcontrib>Hurk, Robert</creatorcontrib><creatorcontrib>Silva, José Roberto V.</creatorcontrib><title>Effects of tumour necrosis factor‐alpha and interleukin‐1 beta on in vitro development of bovine secondary follicles</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>The objective of this study was to determine the effects of TNF‐α and IL‐1β on development and survival of bovine secondary follicle culture in vitro for 18 days. Secondary follicles (~0.2 mm) were isolated from ovarian cortex and individually cultured at 38.5°C, with 5% CO2 in air, for 18 days, in TCM‐199+ alone (cultured control) or supplemented with 10 ng/ml IL‐1β, 10 ng/ml TNF‐α or both TNF‐α and IL‐1β. The effects of these treatments on growth, follicular survival, antrum formation, viability, ultrastructure and mRNA levels for GDF‐9, c‐MOS, H1foo and Cyclin B1 were evaluated. The results showed that addition of TNF‐α to culture medium increased follicular diameter and rate of antrum formation, whereas that of IL‐1β and a mixture of IL‐1β and TNF‐α did not do so. Ultrastructural analysis showed that, among the tested cytokine treatments, follicles cultured in the presence of TNF‐α had the best‐preserved oocytes and granulosa cells. The presence of TNF‐α, IL‐1β or both did not influence the expression of mRNAs analysed. In conclusion, in contrast to IL‐1β, TNF‐α promotes growth of and antrum formation in in vitro cultured bovine secondary follicles, while their ultrastructure and viability were maintained.</description><subject>Animals</subject><subject>Carbon dioxide</subject><subject>Cattle - physiology</subject><subject>Cell culture</subject><subject>cow</subject><subject>culture</subject><subject>Cyclin B1</subject><subject>Cyclin B1 - genetics</subject><subject>Cyclin B1 - metabolism</subject><subject>Female</subject><subject>Follicles</subject><subject>gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Granulosa cells</subject><subject>Growth Differentiation Factor 9 - genetics</subject><subject>Growth Differentiation Factor 9 - metabolism</subject><subject>Histones - genetics</subject><subject>Histones - metabolism</subject><subject>IL-1β</subject><subject>Interleukin-1beta - administration & dosage</subject><subject>Interleukin-1beta - pharmacology</subject><subject>Interleukins</subject><subject>mRNA</subject><subject>Oocytes</subject><subject>Ovarian Follicle - drug effects</subject><subject>Ovarian Follicle - growth & development</subject><subject>ovarian follicles</subject><subject>Proto-Oncogene Proteins c-mos - genetics</subject><subject>Proto-Oncogene Proteins c-mos - metabolism</subject><subject>Survival</subject><subject>Tissue Culture Techniques - veterinary</subject><subject>Tumor necrosis factor</subject><subject>Tumor Necrosis Factor-alpha - administration & dosage</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumors</subject><subject>Ultrastructure</subject><subject>Viability</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9KHTEUh0Op1Kvtoi9QAt3o4moymZkkS7FqBUEQux7y54TGZpLbZOZadz6Cz-iTNLdXuyg0mwOHj4-c3w-hj5Qc0fqOs1VHlFEp36AFbZlcko7Rt2hBJOuXPe_FLtor5Y4Q2gnO36HdRsqWMdkt0K8z58BMBSeHp3lMc8YRTE7FF-yUmVJ-fnxSYfVdYRUt9nGCHGD-4WPdU6xhUjjFusdrP-WELawhpNUIcdoodVr7CLiASdGq_IBdCsGbAOU92nEqFPjwMvfRt_Oz29Ovy6vri8vTk6ulYV29hDEFohWM867lUhDDQGptLG0JQN-oRipOGuGstZo70RKtCYGmIZ0zRFvC9tHB1rvK6ecMZRpGXwyEoCKkuQyVlF3XUMEr-vkf9K7mEevvKtULLvuWbYSHW2qTUsnghlX2Y71toGTY1DHUOoY_dVT204tx1iPYv-Rr_hU43gL3PsDD_03DzZeTrfI3HxyW_A</recordid><startdate>201808</startdate><enddate>201808</enddate><creator>Paulino, Lais R. F. M.</creator><creator>Cunha, Ellen V.</creator><creator>Barbalho Silva, Anderson W.</creator><creator>Souza, Glaucinete B.</creator><creator>Lopes, Ewerton P. F.</creator><creator>Donato, Mariana A. M.</creator><creator>Peixoto, Cristina A.</creator><creator>Matos‐Brito, Bruno G.</creator><creator>Hurk, Robert</creator><creator>Silva, José Roberto V.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5970-6177</orcidid></search><sort><creationdate>201808</creationdate><title>Effects of tumour necrosis factor‐alpha and interleukin‐1 beta on in vitro development of bovine secondary follicles</title><author>Paulino, Lais R. F. M. ; Cunha, Ellen V. ; Barbalho Silva, Anderson W. ; Souza, Glaucinete B. ; Lopes, Ewerton P. F. ; Donato, Mariana A. 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F. M.</creatorcontrib><creatorcontrib>Cunha, Ellen V.</creatorcontrib><creatorcontrib>Barbalho Silva, Anderson W.</creatorcontrib><creatorcontrib>Souza, Glaucinete B.</creatorcontrib><creatorcontrib>Lopes, Ewerton P. F.</creatorcontrib><creatorcontrib>Donato, Mariana A. M.</creatorcontrib><creatorcontrib>Peixoto, Cristina A.</creatorcontrib><creatorcontrib>Matos‐Brito, Bruno G.</creatorcontrib><creatorcontrib>Hurk, Robert</creatorcontrib><creatorcontrib>Silva, José Roberto V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paulino, Lais R. F. M.</au><au>Cunha, Ellen V.</au><au>Barbalho Silva, Anderson W.</au><au>Souza, Glaucinete B.</au><au>Lopes, Ewerton P. F.</au><au>Donato, Mariana A. M.</au><au>Peixoto, Cristina A.</au><au>Matos‐Brito, Bruno G.</au><au>Hurk, Robert</au><au>Silva, José Roberto V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of tumour necrosis factor‐alpha and interleukin‐1 beta on in vitro development of bovine secondary follicles</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2018-08</date><risdate>2018</risdate><volume>53</volume><issue>4</issue><spage>997</spage><epage>1005</epage><pages>997-1005</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>The objective of this study was to determine the effects of TNF‐α and IL‐1β on development and survival of bovine secondary follicle culture in vitro for 18 days. Secondary follicles (~0.2 mm) were isolated from ovarian cortex and individually cultured at 38.5°C, with 5% CO2 in air, for 18 days, in TCM‐199+ alone (cultured control) or supplemented with 10 ng/ml IL‐1β, 10 ng/ml TNF‐α or both TNF‐α and IL‐1β. The effects of these treatments on growth, follicular survival, antrum formation, viability, ultrastructure and mRNA levels for GDF‐9, c‐MOS, H1foo and Cyclin B1 were evaluated. The results showed that addition of TNF‐α to culture medium increased follicular diameter and rate of antrum formation, whereas that of IL‐1β and a mixture of IL‐1β and TNF‐α did not do so. Ultrastructural analysis showed that, among the tested cytokine treatments, follicles cultured in the presence of TNF‐α had the best‐preserved oocytes and granulosa cells. The presence of TNF‐α, IL‐1β or both did not influence the expression of mRNAs analysed. In conclusion, in contrast to IL‐1β, TNF‐α promotes growth of and antrum formation in in vitro cultured bovine secondary follicles, while their ultrastructure and viability were maintained.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>29943395</pmid><doi>10.1111/rda.13199</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5970-6177</orcidid></addata></record> |
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| ispartof | Reproduction in domestic animals, 2018-08, Vol.53 (4), p.997-1005 |
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| subjects | Animals Carbon dioxide Cattle - physiology Cell culture cow culture Cyclin B1 Cyclin B1 - genetics Cyclin B1 - metabolism Female Follicles gene expression Gene Expression Regulation - drug effects Granulosa cells Growth Differentiation Factor 9 - genetics Growth Differentiation Factor 9 - metabolism Histones - genetics Histones - metabolism IL-1β Interleukin-1beta - administration & dosage Interleukin-1beta - pharmacology Interleukins mRNA Oocytes Ovarian Follicle - drug effects Ovarian Follicle - growth & development ovarian follicles Proto-Oncogene Proteins c-mos - genetics Proto-Oncogene Proteins c-mos - metabolism Survival Tissue Culture Techniques - veterinary Tumor necrosis factor Tumor Necrosis Factor-alpha - administration & dosage Tumor Necrosis Factor-alpha - pharmacology Tumor necrosis factor-TNF Tumors Ultrastructure Viability |
| title | Effects of tumour necrosis factor‐alpha and interleukin‐1 beta on in vitro development of bovine secondary follicles |
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