Establishment and characterization of novel patient-derived osteosarcoma xenograft and cell line
Osteosarcoma is an aggressive mesenchymal malignancy of the bone. Patient-derived models are essential tools for elucidating the molecular mechanisms associated with poor prognosis and the development of novel anticancer drugs. This study described the establishment of a patient-derived cancer model...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Animal 2018-08, Vol.54 (7), p.528-536 |
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| creator | Kito, Fusako Oyama, Rieko Sakumoto, Marimu Takahashi, Mami Shiozawa, Kumiko Qiao, Zhiwei Sakamoto, Hiromi Hirose, Takeshi Setsu, Nokitaka Yoshida, Akihiko Kawai, Akira Kondo, Tadashi |
| description | Osteosarcoma is an aggressive mesenchymal malignancy of the bone. Patient-derived models are essential tools for elucidating the molecular mechanisms associated with poor prognosis and the development of novel anticancer drugs. This study described the establishment of a patient-derived cancer model of osteosarcoma. Primary osteosarcoma tumor tissues were obtained from an osteosarcoma patient and inoculated in the skin of immunodeficient mice, followed by transplantation to other mice upon growth. Cells were maintained in monolayer cultures, and the capability of spheroid formation was assessed by seeding the cells on culture dishes. The invasion ability of cells was monitored by Matrigel assay, and genomic and proteomic backgrounds were examined by mass spectrometry. A cell line was established from patient-derived tumors and showed similar histology to that of the primary tumor tissue. Additionally, these cells formed spheroids on low-attachment tissue-culture dishes and exhibited invasive capabilities, and we confirmed that the genomic backgrounds were similar between patient-derived xenograft tumors and the cell line. Furthermore, the prateome of the patient-derived tumors and the cells exhibited similar, but not identical, patterns to that of the original tumor tissue. Our results indicated that this patient-derived xenograft model and cell line would be useful resources for osteosarcoma research. |
| doi_str_mv | 10.1007/s11626-018-0274-2 |
| format | Article |
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Patient-derived models are essential tools for elucidating the molecular mechanisms associated with poor prognosis and the development of novel anticancer drugs. This study described the establishment of a patient-derived cancer model of osteosarcoma. Primary osteosarcoma tumor tissues were obtained from an osteosarcoma patient and inoculated in the skin of immunodeficient mice, followed by transplantation to other mice upon growth. Cells were maintained in monolayer cultures, and the capability of spheroid formation was assessed by seeding the cells on culture dishes. The invasion ability of cells was monitored by Matrigel assay, and genomic and proteomic backgrounds were examined by mass spectrometry. A cell line was established from patient-derived tumors and showed similar histology to that of the primary tumor tissue. Additionally, these cells formed spheroids on low-attachment tissue-culture dishes and exhibited invasive capabilities, and we confirmed that the genomic backgrounds were similar between patient-derived xenograft tumors and the cell line. Furthermore, the prateome of the patient-derived tumors and the cells exhibited similar, but not identical, patterns to that of the original tumor tissue. Our results indicated that this patient-derived xenograft model and cell line would be useful resources for osteosarcoma research.</description><identifier>ISSN: 1071-2690</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/s11626-018-0274-2</identifier><identifier>PMID: 29943355</identifier><language>eng</language><publisher>New York: Springer Science & Business Media LLC</publisher><subject>Animal Genetics and Genomics ; Antineoplastic drugs ; Antitumor agents ; Biocompatibility ; Biomedical and Life Sciences ; Bone cancer ; Cancer therapies ; Cell Biology ; Cell Culture ; Chemotherapy ; Developmental Biology ; Drug development ; Drugs ; Epidemiology ; ESTABLISHMENT OF CELL LINES ; Experiments ; Genes ; Genomes ; Genomics ; Histology ; Immunodeficiency ; Immunosuppressive agents ; Invasiveness ; Life Sciences ; Malignancy ; Mass spectrometry ; Mass spectroscopy ; Medical prognosis ; Mesenchyme ; Metastasis ; Mice ; Molecular modelling ; Osteosarcoma ; Patients ; Proteomes ; Proteomics ; RNA polymerase ; Sarcoma ; Skin ; Spheroids ; Stem Cells ; Tissue culture ; Tissues ; Transplantation ; Transplants & implants ; Tumors ; Xenografts ; Xenotransplantation</subject><ispartof>In vitro cellular & developmental biology. Animal, 2018-08, Vol.54 (7), p.528-536</ispartof><rights>2018 Society for In Vitro Biology</rights><rights>The Society for In Vitro Biology 2018</rights><rights>Copyright Society for In Vitro Biology Aug 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-2278baed7a6b0260b0032e6f7a2bbd5f2829995ab000be1271089f797d3627463</citedby><cites>FETCH-LOGICAL-c468t-2278baed7a6b0260b0032e6f7a2bbd5f2829995ab000be1271089f797d3627463</cites><orcidid>0000-0001-6405-7792</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/45180178$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/45180178$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,41464,42533,51294,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29943355$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kito, Fusako</creatorcontrib><creatorcontrib>Oyama, Rieko</creatorcontrib><creatorcontrib>Sakumoto, Marimu</creatorcontrib><creatorcontrib>Takahashi, Mami</creatorcontrib><creatorcontrib>Shiozawa, Kumiko</creatorcontrib><creatorcontrib>Qiao, Zhiwei</creatorcontrib><creatorcontrib>Sakamoto, Hiromi</creatorcontrib><creatorcontrib>Hirose, Takeshi</creatorcontrib><creatorcontrib>Setsu, Nokitaka</creatorcontrib><creatorcontrib>Yoshida, Akihiko</creatorcontrib><creatorcontrib>Kawai, Akira</creatorcontrib><creatorcontrib>Kondo, Tadashi</creatorcontrib><title>Establishment and characterization of novel patient-derived osteosarcoma xenograft and cell line</title><title>In vitro cellular & developmental biology. Animal</title><addtitle>In Vitro Cell.Dev.Biol.-Animal</addtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>Osteosarcoma is an aggressive mesenchymal malignancy of the bone. Patient-derived models are essential tools for elucidating the molecular mechanisms associated with poor prognosis and the development of novel anticancer drugs. This study described the establishment of a patient-derived cancer model of osteosarcoma. Primary osteosarcoma tumor tissues were obtained from an osteosarcoma patient and inoculated in the skin of immunodeficient mice, followed by transplantation to other mice upon growth. Cells were maintained in monolayer cultures, and the capability of spheroid formation was assessed by seeding the cells on culture dishes. The invasion ability of cells was monitored by Matrigel assay, and genomic and proteomic backgrounds were examined by mass spectrometry. A cell line was established from patient-derived tumors and showed similar histology to that of the primary tumor tissue. Additionally, these cells formed spheroids on low-attachment tissue-culture dishes and exhibited invasive capabilities, and we confirmed that the genomic backgrounds were similar between patient-derived xenograft tumors and the cell line. Furthermore, the prateome of the patient-derived tumors and the cells exhibited similar, but not identical, patterns to that of the original tumor tissue. Our results indicated that this patient-derived xenograft model and cell line would be useful resources for osteosarcoma research.</description><subject>Animal Genetics and Genomics</subject><subject>Antineoplastic drugs</subject><subject>Antitumor agents</subject><subject>Biocompatibility</subject><subject>Biomedical and Life Sciences</subject><subject>Bone cancer</subject><subject>Cancer therapies</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Chemotherapy</subject><subject>Developmental Biology</subject><subject>Drug development</subject><subject>Drugs</subject><subject>Epidemiology</subject><subject>ESTABLISHMENT OF CELL LINES</subject><subject>Experiments</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Histology</subject><subject>Immunodeficiency</subject><subject>Immunosuppressive agents</subject><subject>Invasiveness</subject><subject>Life Sciences</subject><subject>Malignancy</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical prognosis</subject><subject>Mesenchyme</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Molecular modelling</subject><subject>Osteosarcoma</subject><subject>Patients</subject><subject>Proteomes</subject><subject>Proteomics</subject><subject>RNA polymerase</subject><subject>Sarcoma</subject><subject>Skin</subject><subject>Spheroids</subject><subject>Stem Cells</subject><subject>Tissue culture</subject><subject>Tissues</subject><subject>Transplantation</subject><subject>Transplants & 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and characterization of novel patient-derived osteosarcoma xenograft and cell line</title><author>Kito, Fusako ; Oyama, Rieko ; Sakumoto, Marimu ; Takahashi, Mami ; Shiozawa, Kumiko ; Qiao, Zhiwei ; Sakamoto, Hiromi ; Hirose, Takeshi ; Setsu, Nokitaka ; Yoshida, Akihiko ; Kawai, Akira ; Kondo, Tadashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-2278baed7a6b0260b0032e6f7a2bbd5f2829995ab000be1271089f797d3627463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal Genetics and Genomics</topic><topic>Antineoplastic drugs</topic><topic>Antitumor agents</topic><topic>Biocompatibility</topic><topic>Biomedical and Life Sciences</topic><topic>Bone cancer</topic><topic>Cancer therapies</topic><topic>Cell Biology</topic><topic>Cell Culture</topic><topic>Chemotherapy</topic><topic>Developmental Biology</topic><topic>Drug 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The invasion ability of cells was monitored by Matrigel assay, and genomic and proteomic backgrounds were examined by mass spectrometry. A cell line was established from patient-derived tumors and showed similar histology to that of the primary tumor tissue. Additionally, these cells formed spheroids on low-attachment tissue-culture dishes and exhibited invasive capabilities, and we confirmed that the genomic backgrounds were similar between patient-derived xenograft tumors and the cell line. Furthermore, the prateome of the patient-derived tumors and the cells exhibited similar, but not identical, patterns to that of the original tumor tissue. 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| ispartof | In vitro cellular & developmental biology. Animal, 2018-08, Vol.54 (7), p.528-536 |
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| subjects | Animal Genetics and Genomics Antineoplastic drugs Antitumor agents Biocompatibility Biomedical and Life Sciences Bone cancer Cancer therapies Cell Biology Cell Culture Chemotherapy Developmental Biology Drug development Drugs Epidemiology ESTABLISHMENT OF CELL LINES Experiments Genes Genomes Genomics Histology Immunodeficiency Immunosuppressive agents Invasiveness Life Sciences Malignancy Mass spectrometry Mass spectroscopy Medical prognosis Mesenchyme Metastasis Mice Molecular modelling Osteosarcoma Patients Proteomes Proteomics RNA polymerase Sarcoma Skin Spheroids Stem Cells Tissue culture Tissues Transplantation Transplants & implants Tumors Xenografts Xenotransplantation |
| title | Establishment and characterization of novel patient-derived osteosarcoma xenograft and cell line |
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