Trypsin-like enzyme from intestine and pyloric caeca of spotted goatfish ( Pseudupeneus maculatus)

Trypsin-like enzyme was partially purified from the intestine and pyloric caeca of spotted goatfish ( Pseudupeneus maculatus) by a simple three steps procedure: heat treatment, ammonium sulphate precipitation and Sephadex G-75 filtration. The enzymes from the intestine and pyloric caeca were 96- and...

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Veröffentlicht in:Food chemistry 2007, Vol.100 (4), p.1429-1434
Hauptverfasser: Souza, Ana A.G., Amaral, Ian P.G., Santo, Albérico R. Espírito, Carvalho, Luiz B., Bezerra, Ranilson S.
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container_end_page 1434
container_issue 4
container_start_page 1429
container_title Food chemistry
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creator Souza, Ana A.G.
Amaral, Ian P.G.
Santo, Albérico R. Espírito
Carvalho, Luiz B.
Bezerra, Ranilson S.
description Trypsin-like enzyme was partially purified from the intestine and pyloric caeca of spotted goatfish ( Pseudupeneus maculatus) by a simple three steps procedure: heat treatment, ammonium sulphate precipitation and Sephadex G-75 filtration. The enzymes from the intestine and pyloric caeca were 96- and 57.7-fold purified with yield values of 68.1% and 26.1%, respectively. The pyloric caeca enzyme collected from the Sephadex G-75 filtration showed a single band in SDS–PAGE (24.5 kDa). Both enzymes presented identical optima pH (9.0) and temperature (55 °C). After incubation at 45 °C for 30 min, enzymes obtained from intestine remained fully activity while a loss of activity (10%) of enzyme extracted from pyloric caeca was registered. Michaelis constant was not significantly different for trypsin-like enzyme from pyloric caeca (1.82 ± 0.19 mM) and that from the intestine (1.94 ± 0.45 mM) acting on benzoyl- dl-arginine- p-nitroanilide (BAPNA). Finally, their activities were inhibited by the following ions in decreasing order: Al 3+ > Zn 2+ > Hg 2+ = Cu 2+ > Cd 2+. The effects of Ca 2+, Mg 2+, Mn 2+, Ba 2+, K 1+, Li 1+ and Co 2+ showed to be less intensive. The similarities between them provide basis for the proposition of obtaining an attractive protease preparation from the tons of intestine and pyloric caeca, that are usually discarded, from this fish which is an important species exported by North-eastern Brazilian fishery industry.
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source Elsevier ScienceDirect Journals Complete
subjects digestive system
Enzyme
enzyme inhibition
Protease
proteinases
Pseudupeneus maculatus
pyloric cecum
Spotted goatfish
Tropical fish
Trypsin
title Trypsin-like enzyme from intestine and pyloric caeca of spotted goatfish ( Pseudupeneus maculatus)
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