Delivering Inorganic and Organic Reagents and Enzymes from Zein and Developing Optical Sensors
Nowadays, interest in using environmentally friendly materials is increasing in many fields. However, the rational design of sensors with biodegradable materials is a challenge. The main aim of this work is to show the possibility of using zein, a protein from corn, as a biodegradable and low-cost m...
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Veröffentlicht in: | Analytical chemistry (Washington) 2018-07, Vol.90 (14), p.8501-8508 |
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description | Nowadays, interest in using environmentally friendly materials is increasing in many fields. However, the rational design of sensors with biodegradable materials is a challenge. The main aim of this work is to show the possibility of using zein, a protein from corn, as a biodegradable and low-cost material for immobilizing, stabilizing, and delivering different kind of reagents for developing optical sensors. Enzymes, metallic salts, and aromatic and small organic compounds were tested. In addition, different techniques of immobilization, entrapment and adsorption, were used, and different formats, such as solid devices and also multiwell platforms, were proposed. The capacity of zein for immobilizing two reagents together, enzyme and substrate, into a multianalysis format was also shown. Two applications were developed as examples: a colorimetric assay based on a ferric hydroxamate reaction for ester drugs, which was applied in atropine determination in pills, and a fluorimetric enzymatic multiwell-plate biodevice applied in phosphate determination in human serum and urine. Zein demonstrated being not only a green alternative but also a versatile polymer for developing sensors from reagents with different natures in different formats and matrices, thereby resulting in different applications. |
doi_str_mv | 10.1021/acs.analchem.8b01338 |
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However, the rational design of sensors with biodegradable materials is a challenge. The main aim of this work is to show the possibility of using zein, a protein from corn, as a biodegradable and low-cost material for immobilizing, stabilizing, and delivering different kind of reagents for developing optical sensors. Enzymes, metallic salts, and aromatic and small organic compounds were tested. In addition, different techniques of immobilization, entrapment and adsorption, were used, and different formats, such as solid devices and also multiwell platforms, were proposed. The capacity of zein for immobilizing two reagents together, enzyme and substrate, into a multianalysis format was also shown. Two applications were developed as examples: a colorimetric assay based on a ferric hydroxamate reaction for ester drugs, which was applied in atropine determination in pills, and a fluorimetric enzymatic multiwell-plate biodevice applied in phosphate determination in human serum and urine. Zein demonstrated being not only a green alternative but also a versatile polymer for developing sensors from reagents with different natures in different formats and matrices, thereby resulting in different applications.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.8b01338</identifier><identifier>PMID: 29932323</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Analytical chemistry ; Aromatic compounds ; Atropine ; Biodegradability ; Biodegradable materials ; Biodegradation ; Chemistry ; Colorimetry ; Corn ; Entrapment ; Enzymes ; Immobilization ; Optical measuring instruments ; Organic chemicals ; Organic compounds ; Proteins ; Reagents ; Salts ; Sensors ; Substrates ; Urine ; Zein</subject><ispartof>Analytical chemistry (Washington), 2018-07, Vol.90 (14), p.8501-8508</ispartof><rights>Copyright American Chemical Society Jul 17, 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a413t-84f83b55ceaf8c408f4131965e7b0843fa6d7be32e216c2e85f261f34ba298903</citedby><cites>FETCH-LOGICAL-a413t-84f83b55ceaf8c408f4131965e7b0843fa6d7be32e216c2e85f261f34ba298903</cites><orcidid>0000-0002-0980-8298</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.8b01338$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.8b01338$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29932323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bocanegra-Rodríguez, Sara</creatorcontrib><creatorcontrib>Jornet-Martínez, Neus</creatorcontrib><creatorcontrib>Molins-Legua, Carmen</creatorcontrib><creatorcontrib>Campíns-Falcó, Pilar</creatorcontrib><title>Delivering Inorganic and Organic Reagents and Enzymes from Zein and Developing Optical Sensors</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Nowadays, interest in using environmentally friendly materials is increasing in many fields. However, the rational design of sensors with biodegradable materials is a challenge. The main aim of this work is to show the possibility of using zein, a protein from corn, as a biodegradable and low-cost material for immobilizing, stabilizing, and delivering different kind of reagents for developing optical sensors. Enzymes, metallic salts, and aromatic and small organic compounds were tested. In addition, different techniques of immobilization, entrapment and adsorption, were used, and different formats, such as solid devices and also multiwell platforms, were proposed. The capacity of zein for immobilizing two reagents together, enzyme and substrate, into a multianalysis format was also shown. 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Zein demonstrated being not only a green alternative but also a versatile polymer for developing sensors from reagents with different natures in different formats and matrices, thereby resulting in different applications.</description><subject>Analytical chemistry</subject><subject>Aromatic compounds</subject><subject>Atropine</subject><subject>Biodegradability</subject><subject>Biodegradable materials</subject><subject>Biodegradation</subject><subject>Chemistry</subject><subject>Colorimetry</subject><subject>Corn</subject><subject>Entrapment</subject><subject>Enzymes</subject><subject>Immobilization</subject><subject>Optical measuring instruments</subject><subject>Organic chemicals</subject><subject>Organic compounds</subject><subject>Proteins</subject><subject>Reagents</subject><subject>Salts</subject><subject>Sensors</subject><subject>Substrates</subject><subject>Urine</subject><subject>Zein</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kM9PwjAYhhujEUT_A2OWePEy_NpupTsaQCUhIfHHxYNLV77iyNZhCyT41ztgcPBgemjz5nnfJg8h1xS6FBi9V9p3lVWF_sKyKzOgnMsT0qYxg1BIyU5JGwB4yHoALXLh_RyAUqDinLRYknBWnzb5HGCRr9HldhaMbOVmyuY6UHYaTJr3C6oZ2qXfhUP7synRB8ZVZfCBud2lA1xjUS22G5PFMteqCF7R-sr5S3JmVOHxqrk75P1x-NZ_DseTp1H_YRyqiPJlKCMjeRbHGpWROgJp6pgmIsZeBjLiRolpL0POkFGhGcrYMEENjzLFEpkA75C7_e7CVd8r9Mu0zL3GolAWq5VPGcQyBsGZqNHbP-i8WrlaZE1RSuNewsWWivaUdpX3Dk26cHmp3CalkG79p7X_9OA_bfzXtZtmfJWVOD2WDsJrAPbAtn78-N_NXxTpk6c</recordid><startdate>20180717</startdate><enddate>20180717</enddate><creator>Bocanegra-Rodríguez, Sara</creator><creator>Jornet-Martínez, Neus</creator><creator>Molins-Legua, Carmen</creator><creator>Campíns-Falcó, Pilar</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0980-8298</orcidid></search><sort><creationdate>20180717</creationdate><title>Delivering Inorganic and Organic Reagents and Enzymes from Zein and Developing Optical Sensors</title><author>Bocanegra-Rodríguez, Sara ; 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subjects | Analytical chemistry Aromatic compounds Atropine Biodegradability Biodegradable materials Biodegradation Chemistry Colorimetry Corn Entrapment Enzymes Immobilization Optical measuring instruments Organic chemicals Organic compounds Proteins Reagents Salts Sensors Substrates Urine Zein |
title | Delivering Inorganic and Organic Reagents and Enzymes from Zein and Developing Optical Sensors |
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