Identification of NBS-encoding genes linked to black rot resistance in cabbage (Brassica oleracea var. capitata)
Heading cabbage is a nutritionally rich and economically important cruciferous vegetable. Black rot disease, caused by the bacterium Xanthomonas campestris pv. campestris , reduces both the yield and quality of the cabbage head. Nucleotide binding site (NBS)-encoding resistance ( R ) genes play a vi...
Gespeichert in:
| Veröffentlicht in: | Molecular biology reports 2018-10, Vol.45 (5), p.773-785 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 785 |
|---|---|
| container_issue | 5 |
| container_start_page | 773 |
| container_title | Molecular biology reports |
| container_volume | 45 |
| creator | Afrin, Khandker Shazia Rahim, Md Abdur Park, Jong-In Natarajan, Sathishkumar Kim, Hoy-Taek Nou, Ill-Sup |
| description | Heading cabbage is a nutritionally rich and economically important cruciferous vegetable. Black rot disease, caused by the bacterium
Xanthomonas campestris
pv.
campestris
, reduces both the yield and quality of the cabbage head. Nucleotide binding site (NBS)-encoding resistance (
R
) genes play a vital role in the plant immune response to various pathogens. In this study, we analyzed the expression and DNA sequence variation of 31 NBS-encoding genes in cabbage (
Brassica oleracea
var.
capitata
). These genes encoded TIR, NBS, LRR and RPW8 protein domains, all of which are known to be involved in disease resistance. RNA-seq revealed that these 31 genes were differentially expressed in leaf, root, silique, and stem tissues. Furthermore, qPCR analyses revealed that several of these genes were more highly expressed in resistant compared to susceptible cabbage lines, including Bol003711, Bol010135, Bol010559, Bol022784, Bol029866, Bol042121, Bol031422, Bol040045 and Bol042095. Further analysis of these genes promises to yield both practical benefits, such as molecular markers for marker-assisted breeding, and fundamental insights to the mechanisms of resistance to black rot in cabbage. |
| doi_str_mv | 10.1007/s11033-018-4217-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2058502338</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2057712266</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-e4dc562923ac015d5af51d39b50c471187a2df16fafb91821cc9ca3d16cda18c3</originalsourceid><addsrcrecordid>eNp1kTFvFDEQhS0EIpeQH0CDLNGEwsFjr9e7JYkgRIqggNTWrO09OdmzD9sXiX-PTxdAipRqive9N6N5hLwFfg6c648FgEvJOAysE6CZekFWoLRk3aiHl2TFJQfWDQqOyHEpd5zzDrR6TY7EOEpQsluR7bXzsYY5WKwhRZpm-u3iB_PRJhfimq599IUuId57R2ui04L2nuZUafYllIrRehoitThNuPb07CJjKS2NpsVntB7pA-bzpm9DxYof3pBXMy7Fnz7OE3L75fPPy6_s5vvV9eWnG2alFpX5zlnVi1FItByUUzgrcHKcFLedBhg0CjdDP-M8jTAIsHa0KB301iEMVp6Qs0PuNqdfO1-q2YRi_bJg9GlXjOBqUFxIOTT0_RP0Lu1ybNftKa1BiL5vFBwom1Mp2c9mm8MG828D3OzrMIc6TKvD7OswqnnePSbvpo13_xx__98AcQBKk-La5_-rn0_9A20dlJk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2057712266</pqid></control><display><type>article</type><title>Identification of NBS-encoding genes linked to black rot resistance in cabbage (Brassica oleracea var. capitata)</title><source>SpringerNature Journals</source><creator>Afrin, Khandker Shazia ; Rahim, Md Abdur ; Park, Jong-In ; Natarajan, Sathishkumar ; Kim, Hoy-Taek ; Nou, Ill-Sup</creator><creatorcontrib>Afrin, Khandker Shazia ; Rahim, Md Abdur ; Park, Jong-In ; Natarajan, Sathishkumar ; Kim, Hoy-Taek ; Nou, Ill-Sup</creatorcontrib><description>Heading cabbage is a nutritionally rich and economically important cruciferous vegetable. Black rot disease, caused by the bacterium
Xanthomonas campestris
pv.
campestris
, reduces both the yield and quality of the cabbage head. Nucleotide binding site (NBS)-encoding resistance (
R
) genes play a vital role in the plant immune response to various pathogens. In this study, we analyzed the expression and DNA sequence variation of 31 NBS-encoding genes in cabbage (
Brassica oleracea
var.
capitata
). These genes encoded TIR, NBS, LRR and RPW8 protein domains, all of which are known to be involved in disease resistance. RNA-seq revealed that these 31 genes were differentially expressed in leaf, root, silique, and stem tissues. Furthermore, qPCR analyses revealed that several of these genes were more highly expressed in resistant compared to susceptible cabbage lines, including Bol003711, Bol010135, Bol010559, Bol022784, Bol029866, Bol042121, Bol031422, Bol040045 and Bol042095. Further analysis of these genes promises to yield both practical benefits, such as molecular markers for marker-assisted breeding, and fundamental insights to the mechanisms of resistance to black rot in cabbage.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-018-4217-5</identifier><identifier>PMID: 29931534</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Binding sites ; Biomedical and Life Sciences ; Black rot ; Brassica oleracea ; Disease resistance ; Histology ; Immune response ; Life Sciences ; Morphology ; Nucleotide sequence ; Original Article ; Plant immunity ; Ribonucleic acid ; RNA</subject><ispartof>Molecular biology reports, 2018-10, Vol.45 (5), p.773-785</ispartof><rights>Springer Nature B.V. 2018</rights><rights>Molecular Biology Reports is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-e4dc562923ac015d5af51d39b50c471187a2df16fafb91821cc9ca3d16cda18c3</citedby><cites>FETCH-LOGICAL-c372t-e4dc562923ac015d5af51d39b50c471187a2df16fafb91821cc9ca3d16cda18c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-018-4217-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-018-4217-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29931534$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Afrin, Khandker Shazia</creatorcontrib><creatorcontrib>Rahim, Md Abdur</creatorcontrib><creatorcontrib>Park, Jong-In</creatorcontrib><creatorcontrib>Natarajan, Sathishkumar</creatorcontrib><creatorcontrib>Kim, Hoy-Taek</creatorcontrib><creatorcontrib>Nou, Ill-Sup</creatorcontrib><title>Identification of NBS-encoding genes linked to black rot resistance in cabbage (Brassica oleracea var. capitata)</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Heading cabbage is a nutritionally rich and economically important cruciferous vegetable. Black rot disease, caused by the bacterium
Xanthomonas campestris
pv.
campestris
, reduces both the yield and quality of the cabbage head. Nucleotide binding site (NBS)-encoding resistance (
R
) genes play a vital role in the plant immune response to various pathogens. In this study, we analyzed the expression and DNA sequence variation of 31 NBS-encoding genes in cabbage (
Brassica oleracea
var.
capitata
). These genes encoded TIR, NBS, LRR and RPW8 protein domains, all of which are known to be involved in disease resistance. RNA-seq revealed that these 31 genes were differentially expressed in leaf, root, silique, and stem tissues. Furthermore, qPCR analyses revealed that several of these genes were more highly expressed in resistant compared to susceptible cabbage lines, including Bol003711, Bol010135, Bol010559, Bol022784, Bol029866, Bol042121, Bol031422, Bol040045 and Bol042095. Further analysis of these genes promises to yield both practical benefits, such as molecular markers for marker-assisted breeding, and fundamental insights to the mechanisms of resistance to black rot in cabbage.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Binding sites</subject><subject>Biomedical and Life Sciences</subject><subject>Black rot</subject><subject>Brassica oleracea</subject><subject>Disease resistance</subject><subject>Histology</subject><subject>Immune response</subject><subject>Life Sciences</subject><subject>Morphology</subject><subject>Nucleotide sequence</subject><subject>Original Article</subject><subject>Plant immunity</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kTFvFDEQhS0EIpeQH0CDLNGEwsFjr9e7JYkgRIqggNTWrO09OdmzD9sXiX-PTxdAipRqive9N6N5hLwFfg6c648FgEvJOAysE6CZekFWoLRk3aiHl2TFJQfWDQqOyHEpd5zzDrR6TY7EOEpQsluR7bXzsYY5WKwhRZpm-u3iB_PRJhfimq599IUuId57R2ui04L2nuZUafYllIrRehoitThNuPb07CJjKS2NpsVntB7pA-bzpm9DxYof3pBXMy7Fnz7OE3L75fPPy6_s5vvV9eWnG2alFpX5zlnVi1FItByUUzgrcHKcFLedBhg0CjdDP-M8jTAIsHa0KB301iEMVp6Qs0PuNqdfO1-q2YRi_bJg9GlXjOBqUFxIOTT0_RP0Lu1ybNftKa1BiL5vFBwom1Mp2c9mm8MG828D3OzrMIc6TKvD7OswqnnePSbvpo13_xx__98AcQBKk-La5_-rn0_9A20dlJk</recordid><startdate>20181001</startdate><enddate>20181001</enddate><creator>Afrin, Khandker Shazia</creator><creator>Rahim, Md Abdur</creator><creator>Park, Jong-In</creator><creator>Natarajan, Sathishkumar</creator><creator>Kim, Hoy-Taek</creator><creator>Nou, Ill-Sup</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20181001</creationdate><title>Identification of NBS-encoding genes linked to black rot resistance in cabbage (Brassica oleracea var. capitata)</title><author>Afrin, Khandker Shazia ; Rahim, Md Abdur ; Park, Jong-In ; Natarajan, Sathishkumar ; Kim, Hoy-Taek ; Nou, Ill-Sup</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-e4dc562923ac015d5af51d39b50c471187a2df16fafb91821cc9ca3d16cda18c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Binding sites</topic><topic>Biomedical and Life Sciences</topic><topic>Black rot</topic><topic>Brassica oleracea</topic><topic>Disease resistance</topic><topic>Histology</topic><topic>Immune response</topic><topic>Life Sciences</topic><topic>Morphology</topic><topic>Nucleotide sequence</topic><topic>Original Article</topic><topic>Plant immunity</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Afrin, Khandker Shazia</creatorcontrib><creatorcontrib>Rahim, Md Abdur</creatorcontrib><creatorcontrib>Park, Jong-In</creatorcontrib><creatorcontrib>Natarajan, Sathishkumar</creatorcontrib><creatorcontrib>Kim, Hoy-Taek</creatorcontrib><creatorcontrib>Nou, Ill-Sup</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Afrin, Khandker Shazia</au><au>Rahim, Md Abdur</au><au>Park, Jong-In</au><au>Natarajan, Sathishkumar</au><au>Kim, Hoy-Taek</au><au>Nou, Ill-Sup</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of NBS-encoding genes linked to black rot resistance in cabbage (Brassica oleracea var. capitata)</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2018-10-01</date><risdate>2018</risdate><volume>45</volume><issue>5</issue><spage>773</spage><epage>785</epage><pages>773-785</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Heading cabbage is a nutritionally rich and economically important cruciferous vegetable. Black rot disease, caused by the bacterium
Xanthomonas campestris
pv.
campestris
, reduces both the yield and quality of the cabbage head. Nucleotide binding site (NBS)-encoding resistance (
R
) genes play a vital role in the plant immune response to various pathogens. In this study, we analyzed the expression and DNA sequence variation of 31 NBS-encoding genes in cabbage (
Brassica oleracea
var.
capitata
). These genes encoded TIR, NBS, LRR and RPW8 protein domains, all of which are known to be involved in disease resistance. RNA-seq revealed that these 31 genes were differentially expressed in leaf, root, silique, and stem tissues. Furthermore, qPCR analyses revealed that several of these genes were more highly expressed in resistant compared to susceptible cabbage lines, including Bol003711, Bol010135, Bol010559, Bol022784, Bol029866, Bol042121, Bol031422, Bol040045 and Bol042095. Further analysis of these genes promises to yield both practical benefits, such as molecular markers for marker-assisted breeding, and fundamental insights to the mechanisms of resistance to black rot in cabbage.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>29931534</pmid><doi>10.1007/s11033-018-4217-5</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4851 |
| ispartof | Molecular biology reports, 2018-10, Vol.45 (5), p.773-785 |
| issn | 0301-4851 1573-4978 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2058502338 |
| source | SpringerNature Journals |
| subjects | Animal Anatomy Animal Biochemistry Binding sites Biomedical and Life Sciences Black rot Brassica oleracea Disease resistance Histology Immune response Life Sciences Morphology Nucleotide sequence Original Article Plant immunity Ribonucleic acid RNA |
| title | Identification of NBS-encoding genes linked to black rot resistance in cabbage (Brassica oleracea var. capitata) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T02%3A32%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20NBS-encoding%20genes%20linked%20to%20black%20rot%20resistance%20in%20cabbage%20(Brassica%20oleracea%20var.%20capitata)&rft.jtitle=Molecular%20biology%20reports&rft.au=Afrin,%20Khandker%20Shazia&rft.date=2018-10-01&rft.volume=45&rft.issue=5&rft.spage=773&rft.epage=785&rft.pages=773-785&rft.issn=0301-4851&rft.eissn=1573-4978&rft_id=info:doi/10.1007/s11033-018-4217-5&rft_dat=%3Cproquest_cross%3E2057712266%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2057712266&rft_id=info:pmid/29931534&rfr_iscdi=true |