Parentage identification of Odontobutis potamophlia based on microsatellite DNA markers

Microsatellite loci were used for parentage identification of Odontobutis potamophila in five full-sib families. The combined exclusion probability of the first (E-1P) and the second parent (E-2P) revealed an obvious increase with the increase of number of microsatellite loci. The combined exclusion...

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Veröffentlicht in:Journal of genetics 2018-06, Vol.97 (2), p.563-568
Hauptverfasser: Wang, Peipei, Zhao, Cheng, Chen, Shuqiao, Yin, Shaowu, Wang, Qintao, Li, Zecheng, Zhou, Guoqin
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container_issue 2
container_start_page 563
container_title Journal of genetics
container_volume 97
creator Wang, Peipei
Zhao, Cheng
Chen, Shuqiao
Yin, Shaowu
Wang, Qintao
Li, Zecheng
Zhou, Guoqin
description Microsatellite loci were used for parentage identification of Odontobutis potamophila in five full-sib families. The combined exclusion probability of the first (E-1P) and the second parent (E-2P) revealed an obvious increase with the increase of number of microsatellite loci. The combined exclusion probability based on allele frequency suggested that at least eight microsatellite loci were needed for the identification of the 150 individuals from five families supported by the genetic distance analysis of individuals of these families. The double-blind test results indicated that the candidate individuals could find their correct parents through these loci thus eight microsatellite markers can be used for pedigree analysis of O. potamophlia in breeding industry as well as for future selection studies.
doi_str_mv 10.1007/s12041-018-0933-9
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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerNature Journals; Indian Academy of Sciences
subjects Analysis
Animal Genetics and Genomics
Biomedical and Life Sciences
DNA
Evolutionary Biology
Fishes
Gene frequency
Gene loci
Genetic analysis
Genetic distance
Genetic markers
Genotype & phenotype
Life Sciences
Microbial Genetics and Genomics
Microsatellites
Pedigree
Plant Genetics and Genomics
Research Note
title Parentage identification of Odontobutis potamophlia based on microsatellite DNA markers
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