Isolation and characterization of polymorphic microsatellite loci from an EST library of turbot (Scophthalmus maximus) and cross-species amplification

Isolation and characterization of polymorphic microsatellite loci from an EST library of turbot (Scophthalmus maximus) and cross-species amplification

In the present study, we report 12 polymorphic microsatellite loci developed from a cDNA library from the turbot, Scophthalmus maximus. Observed and expected heterozygosities varied from 0.20 to 1.00 and from 0.18 to 0.78, respectively. No significant linkage disequilibrium between pairs of loci was...

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Veröffentlicht in:Molecular ecology notes 2007-09, Vol.7 (5), p.848-850
Hauptverfasser: CHEN, S.L, MA, H.Y, JIANG, Y, LIAO, X.L, MENG, L
Format: Artikel
Sprache:eng
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cDNA
cross-species amplification
ESTs
Marine
microsatellite
microsatellite repeats
Psetta maxima
Scophthalmus maximus
turbot
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container_end_page 850
container_issue 5
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container_title Molecular ecology notes
container_volume 7
creator CHEN, S.L
MA, H.Y
JIANG, Y
LIAO, X.L
MENG, L
description In the present study, we report 12 polymorphic microsatellite loci developed from a cDNA library from the turbot, Scophthalmus maximus. Observed and expected heterozygosities varied from 0.20 to 1.00 and from 0.18 to 0.78, respectively. No significant linkage disequilibrium between pairs of loci was found, but two loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. Cross-species amplifications of these microsatellites in five additional fish species revealed between five and 11 positive amplifications and between zero and four polymorphic loci per species.
doi_str_mv 10.1111/j.1471-8286.2007.01725.x
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source Wiley Online Library Journals Frontfile Complete
subjects cDNA
cross-species amplification
ESTs
Marine
microsatellite
microsatellite repeats
Psetta maxima
Scophthalmus maximus
turbot
title Isolation and characterization of polymorphic microsatellite loci from an EST library of turbot (Scophthalmus maximus) and cross-species amplification
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