[6]-Gingerol inhibits nitric oxide synthesis in activated J774.1 mouse macrophages and prevents peroxynitrite-induced oxidation and nitration reactions
Reactive nitrogen species (RNS), such as nitric oxide (NO) and its derivatives, e.g. peroxynitrite (ONOO −), have been proposed as being able to influence signal transduction and cause DNA damage, contributing to carcinogenic processes. In this study, the effect of [6]-gingerol, a pungent phenolic c...
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| Veröffentlicht in: | Life sciences (1973) 2003-11, Vol.73 (26), p.3427-3437 |
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| container_title | Life sciences (1973) |
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| creator | Ippoushi, Katsunari Azuma, Keiko Ito, Hidekazu Horie, Hideki Higashio, Hisao |
| description | Reactive nitrogen species (RNS), such as nitric oxide (NO) and its derivatives, e.g. peroxynitrite (ONOO
−), have been proposed as being able to influence signal transduction and cause DNA damage, contributing to carcinogenic processes. In this study, the effect of [6]-gingerol, a pungent phenolic compound present in ginger (
Zingiber officinale Roscoe), on NO synthesis in lipopolysaccharide (LPS)-activated J774.1 macrophages was tested, and the protective ability of this compound against peroxynitrite-mediated oxidation and nitration reactions were evaluated. [6]-Gingerol exhibited dose-dependent inhibition of NO production and significant reduction of inducible NO synthase (iNOS) in LPS-stimulated J774.1 cells. Moreover, [6]-gingerol effectively suppressed peroxynitrite-induced oxidation of dichlorodihydrofluorescein, oxidative single strand breaks in supercoiled pTZ 18U plasmid DNA, and formation of 3-nitrotyrosine in bovine serum albumin (BSA) and J774.1 cells. Our results indicate that [6]-gingerol is a potent inhibitor of NO synthesis and also an effective protector against peroxynitrite-mediated damage. |
| doi_str_mv | 10.1016/j.lfs.2003.06.022 |
| format | Article |
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−), have been proposed as being able to influence signal transduction and cause DNA damage, contributing to carcinogenic processes. In this study, the effect of [6]-gingerol, a pungent phenolic compound present in ginger (
Zingiber officinale Roscoe), on NO synthesis in lipopolysaccharide (LPS)-activated J774.1 macrophages was tested, and the protective ability of this compound against peroxynitrite-mediated oxidation and nitration reactions were evaluated. [6]-Gingerol exhibited dose-dependent inhibition of NO production and significant reduction of inducible NO synthase (iNOS) in LPS-stimulated J774.1 cells. Moreover, [6]-gingerol effectively suppressed peroxynitrite-induced oxidation of dichlorodihydrofluorescein, oxidative single strand breaks in supercoiled pTZ 18U plasmid DNA, and formation of 3-nitrotyrosine in bovine serum albumin (BSA) and J774.1 cells. Our results indicate that [6]-gingerol is a potent inhibitor of NO synthesis and also an effective protector against peroxynitrite-mediated damage.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2003.06.022</identifier><identifier>PMID: 14572883</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Catechols ; Cells, Cultured ; DNA Damage - drug effects ; Dose-Response Relationship, Drug ; Enzyme Induction ; Fatty Alcohols - pharmacology ; Fluoresceins - metabolism ; Ginger ; Gingerol ; Lipopolysaccharides - pharmacology ; Macrophage Activation - drug effects ; Macrophage Activation - physiology ; Macrophages ; Macrophages - drug effects ; Macrophages - enzymology ; Mice ; Nitration ; Nitric oxide ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase - antagonists & inhibitors ; Nitric Oxide Synthase - biosynthesis ; Nitric Oxide Synthase Type II ; Oxidation ; Oxidation-Reduction ; Peroxynitrite ; Peroxynitrous Acid - pharmacology ; Reactive nitrogen species ; Zingiber officinale ; Zingiber officinale Roscoe</subject><ispartof>Life sciences (1973), 2003-11, Vol.73 (26), p.3427-3437</ispartof><rights>2003 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-c710ba1f4c2c9d3079bd23e1d4d04a10565d90630c92a5535940c738ea6624793</citedby><cites>FETCH-LOGICAL-c446t-c710ba1f4c2c9d3079bd23e1d4d04a10565d90630c92a5535940c738ea6624793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.lfs.2003.06.022$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14572883$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ippoushi, Katsunari</creatorcontrib><creatorcontrib>Azuma, Keiko</creatorcontrib><creatorcontrib>Ito, Hidekazu</creatorcontrib><creatorcontrib>Horie, Hideki</creatorcontrib><creatorcontrib>Higashio, Hisao</creatorcontrib><title>[6]-Gingerol inhibits nitric oxide synthesis in activated J774.1 mouse macrophages and prevents peroxynitrite-induced oxidation and nitration reactions</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Reactive nitrogen species (RNS), such as nitric oxide (NO) and its derivatives, e.g. peroxynitrite (ONOO
−), have been proposed as being able to influence signal transduction and cause DNA damage, contributing to carcinogenic processes. In this study, the effect of [6]-gingerol, a pungent phenolic compound present in ginger (
Zingiber officinale Roscoe), on NO synthesis in lipopolysaccharide (LPS)-activated J774.1 macrophages was tested, and the protective ability of this compound against peroxynitrite-mediated oxidation and nitration reactions were evaluated. [6]-Gingerol exhibited dose-dependent inhibition of NO production and significant reduction of inducible NO synthase (iNOS) in LPS-stimulated J774.1 cells. Moreover, [6]-gingerol effectively suppressed peroxynitrite-induced oxidation of dichlorodihydrofluorescein, oxidative single strand breaks in supercoiled pTZ 18U plasmid DNA, and formation of 3-nitrotyrosine in bovine serum albumin (BSA) and J774.1 cells. Our results indicate that [6]-gingerol is a potent inhibitor of NO synthesis and also an effective protector against peroxynitrite-mediated damage.</description><subject>Animals</subject><subject>Catechols</subject><subject>Cells, Cultured</subject><subject>DNA Damage - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Induction</subject><subject>Fatty Alcohols - pharmacology</subject><subject>Fluoresceins - metabolism</subject><subject>Ginger</subject><subject>Gingerol</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Macrophage Activation - drug effects</subject><subject>Macrophage Activation - physiology</subject><subject>Macrophages</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - enzymology</subject><subject>Mice</subject><subject>Nitration</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Nitric Oxide Synthase - biosynthesis</subject><subject>Nitric Oxide Synthase Type II</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>Peroxynitrite</subject><subject>Peroxynitrous Acid - pharmacology</subject><subject>Reactive nitrogen species</subject><subject>Zingiber officinale</subject><subject>Zingiber officinale Roscoe</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhS0EokPhAdggr9glXP8kTsQKVdCCKnUDK4Qsj32n41HiBNsz6jwJr1unMxK7rizrnvNJ5xxC3jOoGbD2064eNqnmAKKGtgbOX5AV61RfQSvYS7IC4LISHJoL8ialHQA0jRKvyQWTjeJdJ1bk3-_2T3Xtwz3GaaA-bP3a50SDz9FbOj14hzQdQ95i8qncqbHZH0xGR38oJWtGx2mfkI7GxmnemntM1ARH54gHDIU0F_DD8YmXsfLB7W3xLmCT_RSexMv19Iu48KeQ3pJXGzMkfHd-L8mvb19_Xt1Ut3fX36--3FZWyjZXVjFYG7aRltveCVD92nGBzEkH0jBo2sb1pQ2wPTdNI5peglWiQ9O2XKpeXJKPJ-4cp797TFmPPlkcBhOwBNOlPNXzjhchOwlLzpQibvQc_WjiUTPQyxp6p8saellDQ6vLGsXz4Qzfr0d0_x3n-ovg80mAJeLBY9TJegylIR_RZu0m_wz-EcSlnQ4</recordid><startdate>20031114</startdate><enddate>20031114</enddate><creator>Ippoushi, Katsunari</creator><creator>Azuma, Keiko</creator><creator>Ito, Hidekazu</creator><creator>Horie, Hideki</creator><creator>Higashio, Hisao</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20031114</creationdate><title>[6]-Gingerol inhibits nitric oxide synthesis in activated J774.1 mouse macrophages and prevents peroxynitrite-induced oxidation and nitration reactions</title><author>Ippoushi, Katsunari ; Azuma, Keiko ; Ito, Hidekazu ; Horie, Hideki ; Higashio, Hisao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-c710ba1f4c2c9d3079bd23e1d4d04a10565d90630c92a5535940c738ea6624793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Catechols</topic><topic>Cells, Cultured</topic><topic>DNA Damage - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme Induction</topic><topic>Fatty Alcohols - pharmacology</topic><topic>Fluoresceins - metabolism</topic><topic>Ginger</topic><topic>Gingerol</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Macrophage Activation - drug effects</topic><topic>Macrophage Activation - physiology</topic><topic>Macrophages</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - enzymology</topic><topic>Mice</topic><topic>Nitration</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>Nitric Oxide Synthase - biosynthesis</topic><topic>Nitric Oxide Synthase Type II</topic><topic>Oxidation</topic><topic>Oxidation-Reduction</topic><topic>Peroxynitrite</topic><topic>Peroxynitrous Acid - pharmacology</topic><topic>Reactive nitrogen species</topic><topic>Zingiber officinale</topic><topic>Zingiber officinale Roscoe</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ippoushi, Katsunari</creatorcontrib><creatorcontrib>Azuma, Keiko</creatorcontrib><creatorcontrib>Ito, Hidekazu</creatorcontrib><creatorcontrib>Horie, Hideki</creatorcontrib><creatorcontrib>Higashio, Hisao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ippoushi, Katsunari</au><au>Azuma, Keiko</au><au>Ito, Hidekazu</au><au>Horie, Hideki</au><au>Higashio, Hisao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>[6]-Gingerol inhibits nitric oxide synthesis in activated J774.1 mouse macrophages and prevents peroxynitrite-induced oxidation and nitration reactions</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2003-11-14</date><risdate>2003</risdate><volume>73</volume><issue>26</issue><spage>3427</spage><epage>3437</epage><pages>3427-3437</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Reactive nitrogen species (RNS), such as nitric oxide (NO) and its derivatives, e.g. peroxynitrite (ONOO
−), have been proposed as being able to influence signal transduction and cause DNA damage, contributing to carcinogenic processes. In this study, the effect of [6]-gingerol, a pungent phenolic compound present in ginger (
Zingiber officinale Roscoe), on NO synthesis in lipopolysaccharide (LPS)-activated J774.1 macrophages was tested, and the protective ability of this compound against peroxynitrite-mediated oxidation and nitration reactions were evaluated. [6]-Gingerol exhibited dose-dependent inhibition of NO production and significant reduction of inducible NO synthase (iNOS) in LPS-stimulated J774.1 cells. Moreover, [6]-gingerol effectively suppressed peroxynitrite-induced oxidation of dichlorodihydrofluorescein, oxidative single strand breaks in supercoiled pTZ 18U plasmid DNA, and formation of 3-nitrotyrosine in bovine serum albumin (BSA) and J774.1 cells. Our results indicate that [6]-gingerol is a potent inhibitor of NO synthesis and also an effective protector against peroxynitrite-mediated damage.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>14572883</pmid><doi>10.1016/j.lfs.2003.06.022</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Catechols Cells, Cultured DNA Damage - drug effects Dose-Response Relationship, Drug Enzyme Induction Fatty Alcohols - pharmacology Fluoresceins - metabolism Ginger Gingerol Lipopolysaccharides - pharmacology Macrophage Activation - drug effects Macrophage Activation - physiology Macrophages Macrophages - drug effects Macrophages - enzymology Mice Nitration Nitric oxide Nitric Oxide - biosynthesis Nitric Oxide Synthase - antagonists & inhibitors Nitric Oxide Synthase - biosynthesis Nitric Oxide Synthase Type II Oxidation Oxidation-Reduction Peroxynitrite Peroxynitrous Acid - pharmacology Reactive nitrogen species Zingiber officinale Zingiber officinale Roscoe |
| title | [6]-Gingerol inhibits nitric oxide synthesis in activated J774.1 mouse macrophages and prevents peroxynitrite-induced oxidation and nitration reactions |
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