Immunological role of CD4+CD28null T lymphocytes, natural killer cells, and interferon-gamma in pediatric patients with sickle cell disease: relation to disease severity and response to therapy
Sickle cell disease (SCD) is associated with alterations in immune phenotypes. CD4 + CD28 null T lymphocytes have pro-inflammatory functions and are linked to vascular diseases. To assess the percentage of CD4 + CD28 null T lymphocytes, natural killer cells (NK), and IFN-gamma levels, we compared 40...
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| Veröffentlicht in: | Immunologic research 2018-08, Vol.66 (4), p.480-490 |
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| creator | ElAlfy, Mohsen Saleh Adly, Amira Abdel Moneam Ebeid, Fatma Soliman ElSayed Eissa, Deena Samir Ismail, Eman Abdel Rahman Mohammed, Yasser Hassan Ahmed, Manar Elsayed Saad, Aya Sayed |
| description | Sickle cell disease (SCD) is associated with alterations in immune phenotypes. CD4
+
CD28
null
T lymphocytes have pro-inflammatory functions and are linked to vascular diseases. To assess the percentage of CD4
+
CD28
null
T lymphocytes, natural killer cells (NK), and IFN-gamma levels, we compared 40 children and adolescents with SCD with 40 healthy controls and evaluated their relation to disease severity and response to therapy. Patients with SCD steady state were studied, focusing on history of frequent vaso-occlusive crisis, hydroxyurea therapy, and IFN-gamma levels. Analysis of CD4
+
CD28
null
T lymphocytes and NK cells was done by flow cytometry. Liver and cardiac iron overload were assessed. CD4
+
CD28
null
T lymphocytes, NK cells, and IFN-gamma levels were significantly higher in patients than controls. Patients with history of frequent vaso-occlusive crisis and those with vascular complications had higher percentage of CD4
+
CD28
null
T lymphocytes and IFN-gamma while levels were significantly lower among hydroxyurea-treated patients. CD4
+
CD28
null
T lymphocytes were positively correlated to transfusional iron input while these cells and IFN-gamma were negatively correlated to cardiac T2* and duration of hydroxyurea therapy. NK cells were correlated to HbS and indirect bilirubin. Increased expression of CD4
+
CD28
null
T lymphocytes highlights their role in immune dysfunction and pathophysiology of SCD complications. |
| doi_str_mv | 10.1007/s12026-018-9010-y |
| format | Article |
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+
CD28
null
T lymphocytes have pro-inflammatory functions and are linked to vascular diseases. To assess the percentage of CD4
+
CD28
null
T lymphocytes, natural killer cells (NK), and IFN-gamma levels, we compared 40 children and adolescents with SCD with 40 healthy controls and evaluated their relation to disease severity and response to therapy. Patients with SCD steady state were studied, focusing on history of frequent vaso-occlusive crisis, hydroxyurea therapy, and IFN-gamma levels. Analysis of CD4
+
CD28
null
T lymphocytes and NK cells was done by flow cytometry. Liver and cardiac iron overload were assessed. CD4
+
CD28
null
T lymphocytes, NK cells, and IFN-gamma levels were significantly higher in patients than controls. Patients with history of frequent vaso-occlusive crisis and those with vascular complications had higher percentage of CD4
+
CD28
null
T lymphocytes and IFN-gamma while levels were significantly lower among hydroxyurea-treated patients. CD4
+
CD28
null
T lymphocytes were positively correlated to transfusional iron input while these cells and IFN-gamma were negatively correlated to cardiac T2* and duration of hydroxyurea therapy. NK cells were correlated to HbS and indirect bilirubin. Increased expression of CD4
+
CD28
null
T lymphocytes highlights their role in immune dysfunction and pathophysiology of SCD complications.</description><identifier>ISSN: 0257-277X</identifier><identifier>EISSN: 1559-0755</identifier><identifier>DOI: 10.1007/s12026-018-9010-y</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adolescents ; Allergology ; Bilirubin ; Biomedical and Life Sciences ; Biomedicine ; CD28 antigen ; CD4 antigen ; Children ; Complications ; Correlation ; Disease control ; Flow cytometry ; Heart ; Hydroxyurea ; Immunology ; Inflammation ; Interferon ; Internal Medicine ; Iron ; Liver ; Lymphocytes ; Lymphocytes T ; Medicine/Public Health ; Natural killer cells ; Original Article ; Patients ; Phenotypes ; Sickle cell disease ; Therapy ; Vascular diseases ; γ-Interferon</subject><ispartof>Immunologic research, 2018-08, Vol.66 (4), p.480-490</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Immunologic Research is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c194y-a1784134b8a606f5ca738fa684be97c8f0a3369f2e4b2c9a15967257cb22efd3</citedby><cites>FETCH-LOGICAL-c194y-a1784134b8a606f5ca738fa684be97c8f0a3369f2e4b2c9a15967257cb22efd3</cites><orcidid>0000-0002-1162-0171</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12026-018-9010-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12026-018-9010-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>ElAlfy, Mohsen Saleh</creatorcontrib><creatorcontrib>Adly, Amira Abdel Moneam</creatorcontrib><creatorcontrib>Ebeid, Fatma Soliman ElSayed</creatorcontrib><creatorcontrib>Eissa, Deena Samir</creatorcontrib><creatorcontrib>Ismail, Eman Abdel Rahman</creatorcontrib><creatorcontrib>Mohammed, Yasser Hassan</creatorcontrib><creatorcontrib>Ahmed, Manar Elsayed</creatorcontrib><creatorcontrib>Saad, Aya Sayed</creatorcontrib><title>Immunological role of CD4+CD28null T lymphocytes, natural killer cells, and interferon-gamma in pediatric patients with sickle cell disease: relation to disease severity and response to therapy</title><title>Immunologic research</title><addtitle>Immunol Res</addtitle><description>Sickle cell disease (SCD) is associated with alterations in immune phenotypes. CD4
+
CD28
null
T lymphocytes have pro-inflammatory functions and are linked to vascular diseases. To assess the percentage of CD4
+
CD28
null
T lymphocytes, natural killer cells (NK), and IFN-gamma levels, we compared 40 children and adolescents with SCD with 40 healthy controls and evaluated their relation to disease severity and response to therapy. Patients with SCD steady state were studied, focusing on history of frequent vaso-occlusive crisis, hydroxyurea therapy, and IFN-gamma levels. Analysis of CD4
+
CD28
null
T lymphocytes and NK cells was done by flow cytometry. Liver and cardiac iron overload were assessed. CD4
+
CD28
null
T lymphocytes, NK cells, and IFN-gamma levels were significantly higher in patients than controls. Patients with history of frequent vaso-occlusive crisis and those with vascular complications had higher percentage of CD4
+
CD28
null
T lymphocytes and IFN-gamma while levels were significantly lower among hydroxyurea-treated patients. CD4
+
CD28
null
T lymphocytes were positively correlated to transfusional iron input while these cells and IFN-gamma were negatively correlated to cardiac T2* and duration of hydroxyurea therapy. NK cells were correlated to HbS and indirect bilirubin. Increased expression of CD4
+
CD28
null
T lymphocytes highlights their role in immune dysfunction and pathophysiology of SCD complications.</description><subject>Adolescents</subject><subject>Allergology</subject><subject>Bilirubin</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>CD28 antigen</subject><subject>CD4 antigen</subject><subject>Children</subject><subject>Complications</subject><subject>Correlation</subject><subject>Disease control</subject><subject>Flow cytometry</subject><subject>Heart</subject><subject>Hydroxyurea</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Interferon</subject><subject>Internal Medicine</subject><subject>Iron</subject><subject>Liver</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Medicine/Public Health</subject><subject>Natural killer cells</subject><subject>Original Article</subject><subject>Patients</subject><subject>Phenotypes</subject><subject>Sickle cell disease</subject><subject>Therapy</subject><subject>Vascular diseases</subject><subject>γ-Interferon</subject><issn>0257-277X</issn><issn>1559-0755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kV9rFDEUxQex4Fr7AXwL-CJoNP9mMuObbLUtFPqyD30L2ezNbtpMMiYZZT6e38xsVxEEny4cfufcyz1N85qSD5QQ-TFTRliHCe3xQCjBy7NmRdt2wES27fNmRVgrMZPy_kXzMucHQmgnBF81P2_GcQ7Rx70z2qMUPaBo0fpSvFtfsj7M3qMN8ss4HaJZCuT3KOgyp8o-Ou8hIQPeV1WHHXKhQLKQYsB7PY66CmiCndMlOYMmXRyEktEPVw4oO_NYdx3daOcy6AyfUAJfoRhQiX9ElOE7JFeWpw0J8hRDVStQDpD0tLxqzqz2GS5-z_Nm8_XLZn2Nb--ubtafb7Ghg1iwprIXlIttrzvS2dZoyXuru15sYZCmt0Rz3g2WgdgyM2jaDp2sTzNbxsDu-Hnz9hQ7pfhthlzU6PLxeh0gzlkx0speEslFRd_8gz7EOYV63BPFBtpxXil6okyKOSewakpu1GlRlKhjp-rUqaqdqmOnaqkedvLkyoY9pL_J_zf9AjAsqDA</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>ElAlfy, Mohsen Saleh</creator><creator>Adly, Amira Abdel Moneam</creator><creator>Ebeid, Fatma Soliman ElSayed</creator><creator>Eissa, Deena Samir</creator><creator>Ismail, Eman Abdel Rahman</creator><creator>Mohammed, Yasser Hassan</creator><creator>Ahmed, Manar Elsayed</creator><creator>Saad, Aya Sayed</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1162-0171</orcidid></search><sort><creationdate>20180801</creationdate><title>Immunological role of CD4+CD28null T lymphocytes, natural killer cells, and interferon-gamma in pediatric patients with sickle cell disease: relation to disease severity and response to therapy</title><author>ElAlfy, Mohsen Saleh ; Adly, Amira Abdel Moneam ; Ebeid, Fatma Soliman ElSayed ; Eissa, Deena Samir ; Ismail, Eman Abdel Rahman ; Mohammed, Yasser Hassan ; Ahmed, Manar Elsayed ; Saad, Aya Sayed</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c194y-a1784134b8a606f5ca738fa684be97c8f0a3369f2e4b2c9a15967257cb22efd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adolescents</topic><topic>Allergology</topic><topic>Bilirubin</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>CD28 antigen</topic><topic>CD4 antigen</topic><topic>Children</topic><topic>Complications</topic><topic>Correlation</topic><topic>Disease control</topic><topic>Flow cytometry</topic><topic>Heart</topic><topic>Hydroxyurea</topic><topic>Immunology</topic><topic>Inflammation</topic><topic>Interferon</topic><topic>Internal Medicine</topic><topic>Iron</topic><topic>Liver</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Medicine/Public Health</topic><topic>Natural killer cells</topic><topic>Original Article</topic><topic>Patients</topic><topic>Phenotypes</topic><topic>Sickle cell disease</topic><topic>Therapy</topic><topic>Vascular diseases</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ElAlfy, Mohsen Saleh</creatorcontrib><creatorcontrib>Adly, Amira Abdel Moneam</creatorcontrib><creatorcontrib>Ebeid, Fatma Soliman ElSayed</creatorcontrib><creatorcontrib>Eissa, Deena Samir</creatorcontrib><creatorcontrib>Ismail, Eman Abdel Rahman</creatorcontrib><creatorcontrib>Mohammed, Yasser Hassan</creatorcontrib><creatorcontrib>Ahmed, Manar Elsayed</creatorcontrib><creatorcontrib>Saad, Aya Sayed</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Immunologic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ElAlfy, Mohsen Saleh</au><au>Adly, Amira Abdel Moneam</au><au>Ebeid, Fatma Soliman ElSayed</au><au>Eissa, Deena Samir</au><au>Ismail, Eman Abdel Rahman</au><au>Mohammed, Yasser Hassan</au><au>Ahmed, Manar Elsayed</au><au>Saad, Aya Sayed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunological role of CD4+CD28null T lymphocytes, natural killer cells, and interferon-gamma in pediatric patients with sickle cell disease: relation to disease severity and response to therapy</atitle><jtitle>Immunologic research</jtitle><stitle>Immunol Res</stitle><date>2018-08-01</date><risdate>2018</risdate><volume>66</volume><issue>4</issue><spage>480</spage><epage>490</epage><pages>480-490</pages><issn>0257-277X</issn><eissn>1559-0755</eissn><abstract>Sickle cell disease (SCD) is associated with alterations in immune phenotypes. CD4
+
CD28
null
T lymphocytes have pro-inflammatory functions and are linked to vascular diseases. To assess the percentage of CD4
+
CD28
null
T lymphocytes, natural killer cells (NK), and IFN-gamma levels, we compared 40 children and adolescents with SCD with 40 healthy controls and evaluated their relation to disease severity and response to therapy. Patients with SCD steady state were studied, focusing on history of frequent vaso-occlusive crisis, hydroxyurea therapy, and IFN-gamma levels. Analysis of CD4
+
CD28
null
T lymphocytes and NK cells was done by flow cytometry. Liver and cardiac iron overload were assessed. CD4
+
CD28
null
T lymphocytes, NK cells, and IFN-gamma levels were significantly higher in patients than controls. Patients with history of frequent vaso-occlusive crisis and those with vascular complications had higher percentage of CD4
+
CD28
null
T lymphocytes and IFN-gamma while levels were significantly lower among hydroxyurea-treated patients. CD4
+
CD28
null
T lymphocytes were positively correlated to transfusional iron input while these cells and IFN-gamma were negatively correlated to cardiac T2* and duration of hydroxyurea therapy. NK cells were correlated to HbS and indirect bilirubin. Increased expression of CD4
+
CD28
null
T lymphocytes highlights their role in immune dysfunction and pathophysiology of SCD complications.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s12026-018-9010-y</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-1162-0171</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Immunologic research, 2018-08, Vol.66 (4), p.480-490 |
| issn | 0257-277X 1559-0755 |
| language | eng |
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| source | SpringerLink Journals |
| subjects | Adolescents Allergology Bilirubin Biomedical and Life Sciences Biomedicine CD28 antigen CD4 antigen Children Complications Correlation Disease control Flow cytometry Heart Hydroxyurea Immunology Inflammation Interferon Internal Medicine Iron Liver Lymphocytes Lymphocytes T Medicine/Public Health Natural killer cells Original Article Patients Phenotypes Sickle cell disease Therapy Vascular diseases γ-Interferon |
| title | Immunological role of CD4+CD28null T lymphocytes, natural killer cells, and interferon-gamma in pediatric patients with sickle cell disease: relation to disease severity and response to therapy |
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