A rapid and eco-friendly isothermal amplification microdevice for multiplex detection of foodborne pathogens

In this study, a plastic microdevice based on loop-mediated isothermal amplification (LAMP) was fabricated for the amplification and on-chip fluorescence detection of multiple pathogens. Papers infused with LAMP reagents and specific primers were embedded inside the multiple reaction chambers of the...

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Veröffentlicht in:	Lab on a chip 2018-01, Vol.18 (16), p.2369-2377
Hauptverfasser:	Trinh, Thi Ngoc Diep, Lee, Nae Yoon
Format:	Artikel
Sprache:	eng
Schlagworte:	Centrifugal force Chambers Deoxyribonucleic acid Dinoflagellida - genetics Dinoflagellida - isolation & purification DNA E coli Escherichia coli O157 - genetics Escherichia coli O157 - isolation & purification Fluorescence Food Microbiology Green Chemistry Technology Limit of Detection Microorganisms Microtechnology - instrumentation Nucleic Acid Amplification Techniques - instrumentation Pathogens Point of care testing Primers (coatings) Reagents Salmonella Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Temperature Time Factors
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