Novel plasmid-mediated colistin resistance gene mcr-7.1 in Klebsiella pneumoniae
Abstract Objectives To identify a novel plasmid-mediated colistin resistance gene in Klebsiella pneumoniae isolated from chickens in China. Methods WGS was used to identify a novel colistin resistance gene. The transferability of plasmids carrying mcr-7.1 was investigated by conjugation experiments....
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2018-07, Vol.73 (7), p.1791-1795 |
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| container_title | Journal of antimicrobial chemotherapy |
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| creator | Yang, Yong-Qiang Li, Yun-Xia Lei, Chang-Wei Zhang, An-Yun Wang, Hong-Ning |
| description | Abstract
Objectives
To identify a novel plasmid-mediated colistin resistance gene in Klebsiella pneumoniae isolated from chickens in China.
Methods
WGS was used to identify a novel colistin resistance gene. The transferability of plasmids carrying mcr-7.1 was investigated by conjugation experiments. The expression of the mcr-7.1 gene was examined using an expression vector.
Results
A novel plasmid-mediated colistin resistance gene mcr-7.1, sharing 70% amino acid identity with the mcr-3 gene, was identified in three K. pneumoniae strains isolated from chickens in China. The mcr-7.1 gene was found in an IncI2-type plasmid (pSC20141012) that co-harboured the blaCTX-M-55 gene in one isolate. pSC20141012 can be transferred from K. pneumoniae SC20141012 to Escherichia coli J53Azr, exhibiting a ≥8-fold increase in colistin MIC compared with the recipient E. coli J53Azr.
Conclusions
We identified a novel plasmid-mediated colistin resistance gene named mcr-7.1 in K. pneumoniae in China. The prevalence of mcr-7.1 in various species of human and animal origin needs to be investigated immediately. |
| doi_str_mv | 10.1093/jac/dky111 |
| format | Article |
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Objectives
To identify a novel plasmid-mediated colistin resistance gene in Klebsiella pneumoniae isolated from chickens in China.
Methods
WGS was used to identify a novel colistin resistance gene. The transferability of plasmids carrying mcr-7.1 was investigated by conjugation experiments. The expression of the mcr-7.1 gene was examined using an expression vector.
Results
A novel plasmid-mediated colistin resistance gene mcr-7.1, sharing 70% amino acid identity with the mcr-3 gene, was identified in three K. pneumoniae strains isolated from chickens in China. The mcr-7.1 gene was found in an IncI2-type plasmid (pSC20141012) that co-harboured the blaCTX-M-55 gene in one isolate. pSC20141012 can be transferred from K. pneumoniae SC20141012 to Escherichia coli J53Azr, exhibiting a ≥8-fold increase in colistin MIC compared with the recipient E. coli J53Azr.
Conclusions
We identified a novel plasmid-mediated colistin resistance gene named mcr-7.1 in K. pneumoniae in China. The prevalence of mcr-7.1 in various species of human and animal origin needs to be investigated immediately.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dky111</identifier><identifier>PMID: 29912417</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><ispartof>Journal of antimicrobial chemotherapy, 2018-07, Vol.73 (7), p.1791-1795</ispartof><rights>The Author(s) 2018. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please email: journals.permissions@oup.com. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-5d135da0754aa0ebbf6c4be5867a9bdc5a41f1a9b89cc4fb595df8d11de29ad73</citedby><cites>FETCH-LOGICAL-c460t-5d135da0754aa0ebbf6c4be5867a9bdc5a41f1a9b89cc4fb595df8d11de29ad73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29912417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Yong-Qiang</creatorcontrib><creatorcontrib>Li, Yun-Xia</creatorcontrib><creatorcontrib>Lei, Chang-Wei</creatorcontrib><creatorcontrib>Zhang, An-Yun</creatorcontrib><creatorcontrib>Wang, Hong-Ning</creatorcontrib><title>Novel plasmid-mediated colistin resistance gene mcr-7.1 in Klebsiella pneumoniae</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>Abstract
Objectives
To identify a novel plasmid-mediated colistin resistance gene in Klebsiella pneumoniae isolated from chickens in China.
Methods
WGS was used to identify a novel colistin resistance gene. The transferability of plasmids carrying mcr-7.1 was investigated by conjugation experiments. The expression of the mcr-7.1 gene was examined using an expression vector.
Results
A novel plasmid-mediated colistin resistance gene mcr-7.1, sharing 70% amino acid identity with the mcr-3 gene, was identified in three K. pneumoniae strains isolated from chickens in China. The mcr-7.1 gene was found in an IncI2-type plasmid (pSC20141012) that co-harboured the blaCTX-M-55 gene in one isolate. pSC20141012 can be transferred from K. pneumoniae SC20141012 to Escherichia coli J53Azr, exhibiting a ≥8-fold increase in colistin MIC compared with the recipient E. coli J53Azr.
Conclusions
We identified a novel plasmid-mediated colistin resistance gene named mcr-7.1 in K. pneumoniae in China. The prevalence of mcr-7.1 in various species of human and animal origin needs to be investigated immediately.</description><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLxDAUhYMoTh3d-AOkG0GEzuS2SdssZfCFg7rQdUmTW8nYl0krzL83Q0eXru6B-3E4fIScA10AFclyI9VSf24B4IAEwFIaxVTAIQloQnmUMZ7MyIlzG0ppytP8mMxiISBmkAXk9bn7xjrsa-kao6MGtZED6lB1tXGDaUOLzgfZKgw_sMWwUTbKFhD611ONpTNY1zLsWxybrjUST8lRJWuHZ_s7J-93t2-rh2j9cv-4ullHyg8cIq4h4VrSjDMpKZZllSpWIs_TTIpSKy4ZVOBjLpRiVckF11WuATTGQuosmZOrqbe33deIbiga49RuTIvd6IqY8jRLqWDCo9cTqmznnMWq6K1ppN0WQIudwcIbLCaDHr7Y946lt_GH_irzwOUEdGP_X9EPRb16Vw</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Yang, Yong-Qiang</creator><creator>Li, Yun-Xia</creator><creator>Lei, Chang-Wei</creator><creator>Zhang, An-Yun</creator><creator>Wang, Hong-Ning</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180701</creationdate><title>Novel plasmid-mediated colistin resistance gene mcr-7.1 in Klebsiella pneumoniae</title><author>Yang, Yong-Qiang ; Li, Yun-Xia ; Lei, Chang-Wei ; Zhang, An-Yun ; Wang, Hong-Ning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-5d135da0754aa0ebbf6c4be5867a9bdc5a41f1a9b89cc4fb595df8d11de29ad73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Yong-Qiang</creatorcontrib><creatorcontrib>Li, Yun-Xia</creatorcontrib><creatorcontrib>Lei, Chang-Wei</creatorcontrib><creatorcontrib>Zhang, An-Yun</creatorcontrib><creatorcontrib>Wang, Hong-Ning</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Yong-Qiang</au><au>Li, Yun-Xia</au><au>Lei, Chang-Wei</au><au>Zhang, An-Yun</au><au>Wang, Hong-Ning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel plasmid-mediated colistin resistance gene mcr-7.1 in Klebsiella pneumoniae</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2018-07-01</date><risdate>2018</risdate><volume>73</volume><issue>7</issue><spage>1791</spage><epage>1795</epage><pages>1791-1795</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><abstract>Abstract
Objectives
To identify a novel plasmid-mediated colistin resistance gene in Klebsiella pneumoniae isolated from chickens in China.
Methods
WGS was used to identify a novel colistin resistance gene. The transferability of plasmids carrying mcr-7.1 was investigated by conjugation experiments. The expression of the mcr-7.1 gene was examined using an expression vector.
Results
A novel plasmid-mediated colistin resistance gene mcr-7.1, sharing 70% amino acid identity with the mcr-3 gene, was identified in three K. pneumoniae strains isolated from chickens in China. The mcr-7.1 gene was found in an IncI2-type plasmid (pSC20141012) that co-harboured the blaCTX-M-55 gene in one isolate. pSC20141012 can be transferred from K. pneumoniae SC20141012 to Escherichia coli J53Azr, exhibiting a ≥8-fold increase in colistin MIC compared with the recipient E. coli J53Azr.
Conclusions
We identified a novel plasmid-mediated colistin resistance gene named mcr-7.1 in K. pneumoniae in China. The prevalence of mcr-7.1 in various species of human and animal origin needs to be investigated immediately.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>29912417</pmid><doi>10.1093/jac/dky111</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| title | Novel plasmid-mediated colistin resistance gene mcr-7.1 in Klebsiella pneumoniae |
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