Ergothioneine production using Methylobacterium species, yeast, and fungi
Ergothioneine (EGT) is a sulfur-containing, anti-oxidative amino acid derived from histidine. EGT is synthesized in bacteria and fungi but not in animals and plants, and is now recognized as important for human health. Its cost-effective fermentative production has not been elucidated due to the lac...
Gespeichert in:
| Veröffentlicht in: | Journal of bioscience and bioengineering 2018-12, Vol.126 (6), p.715-722 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 722 |
|---|---|
| container_issue | 6 |
| container_start_page | 715 |
| container_title | Journal of bioscience and bioengineering |
| container_volume | 126 |
| creator | Fujitani, Yoshiko Alamgir, Kabir Md Tani, Akio |
| description | Ergothioneine (EGT) is a sulfur-containing, anti-oxidative amino acid derived from histidine. EGT is synthesized in bacteria and fungi but not in animals and plants, and is now recognized as important for human health. Its cost-effective fermentative production has not been elucidated due to the lack of information for productive microorganisms. In this study, we doubled the gene copy for EGT synthesis and deleted the histidine ammonia-lyase gene in a potent EGT-producing methylotrophic bacterium Methylobacterium aquaticum strain 22A, and optimized its culture conditions, resulting in increased EGT production of 7.0 mg EGT/g dry cell weight and 100 μg EGT/5 mL/7 days. In addition, through screening we found EGT-producing eukaryotic strains of Aureobasidium pullulans and Rhodotorula mucilaginosa, which can produce 1.0 and 3.2 mg EGT/g dry cell weight, 70 and 120 μg EGT/5 mL/7 days, respectively. This study proposes practical uses of potent EGT-producing recombinant Methylobacterium species and non-recombinant yeast and fungal strains. |
| doi_str_mv | 10.1016/j.jbiosc.2018.05.021 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2056759855</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1389172318302950</els_id><sourcerecordid>2056759855</sourcerecordid><originalsourceid>FETCH-LOGICAL-c501t-68943d892b51f3518737c3b0bf7962f08163e35196a24acd8c1a48027d7460b63</originalsourceid><addsrcrecordid>eNp9kD1PwzAQhi0EoqXwDxDKyNAE24lje0FCVYFKRSwwW45zaR2lSbETpP57XKUwMt2H3rv37kHoluCEYJI_1Eld2M6bhGIiEswSTMkZmpI043GWUXJ-zIWMCafpBF15X2NMOObkEk2olGGHkFO0WrpN129t14JtIdq7rhxMH8po8LbdRG_Qbw9NV2jTg7PDLvJ7MBb8PDqA9v080m0ZVUO7sdfootKNh5tTnKHP5-XH4jVev7-sFk_r2DBM-jgXMktLIWnBSJUyInjKTVrgouIypxUWJE8h9GWuaaZNKQzRmcCUlzzLcZGnM3Q_7g23fg3ge7Wz3kDT6Ba6wSuKWc6ZFIwFaTZKjeu8d1CpvbM77Q6KYHWEqGo1QlRHiAozFSCGsbuTw1DsoPwb-qUWBI-jAMKf3xac8oFJa6C0Dkyvys7-7_ADjSqD1Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2056759855</pqid></control><display><type>article</type><title>Ergothioneine production using Methylobacterium species, yeast, and fungi</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Fujitani, Yoshiko ; Alamgir, Kabir Md ; Tani, Akio</creator><creatorcontrib>Fujitani, Yoshiko ; Alamgir, Kabir Md ; Tani, Akio</creatorcontrib><description>Ergothioneine (EGT) is a sulfur-containing, anti-oxidative amino acid derived from histidine. EGT is synthesized in bacteria and fungi but not in animals and plants, and is now recognized as important for human health. Its cost-effective fermentative production has not been elucidated due to the lack of information for productive microorganisms. In this study, we doubled the gene copy for EGT synthesis and deleted the histidine ammonia-lyase gene in a potent EGT-producing methylotrophic bacterium Methylobacterium aquaticum strain 22A, and optimized its culture conditions, resulting in increased EGT production of 7.0 mg EGT/g dry cell weight and 100 μg EGT/5 mL/7 days. In addition, through screening we found EGT-producing eukaryotic strains of Aureobasidium pullulans and Rhodotorula mucilaginosa, which can produce 1.0 and 3.2 mg EGT/g dry cell weight, 70 and 120 μg EGT/5 mL/7 days, respectively. This study proposes practical uses of potent EGT-producing recombinant Methylobacterium species and non-recombinant yeast and fungal strains.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/j.jbiosc.2018.05.021</identifier><identifier>PMID: 29910189</identifier><language>eng</language><publisher>Japan: Elsevier B.V</publisher><subject>Animals ; Anti-oxidative amino acid ; Antioxidants - metabolism ; Aureobasidium pullulans ; Ergothioneine ; Ergothioneine - biosynthesis ; Fungi - genetics ; Fungi - metabolism ; Histidine - metabolism ; Humans ; Metabolic Engineering ; Methanol ; Methanol - metabolism ; Methylobacterium - genetics ; Methylobacterium - metabolism ; Methylobacterium species ; Organisms, Genetically Modified ; Oxidation-Reduction ; Rhodotorula - genetics ; Rhodotorula - growth & development ; Rhodotorula - metabolism ; Rhodotorula mucilaginosa ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Yeasts - genetics ; Yeasts - metabolism</subject><ispartof>Journal of bioscience and bioengineering, 2018-12, Vol.126 (6), p.715-722</ispartof><rights>2018 The Society for Biotechnology, Japan</rights><rights>Copyright © 2018 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-68943d892b51f3518737c3b0bf7962f08163e35196a24acd8c1a48027d7460b63</citedby><cites>FETCH-LOGICAL-c501t-68943d892b51f3518737c3b0bf7962f08163e35196a24acd8c1a48027d7460b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1389172318302950$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29910189$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujitani, Yoshiko</creatorcontrib><creatorcontrib>Alamgir, Kabir Md</creatorcontrib><creatorcontrib>Tani, Akio</creatorcontrib><title>Ergothioneine production using Methylobacterium species, yeast, and fungi</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>Ergothioneine (EGT) is a sulfur-containing, anti-oxidative amino acid derived from histidine. EGT is synthesized in bacteria and fungi but not in animals and plants, and is now recognized as important for human health. Its cost-effective fermentative production has not been elucidated due to the lack of information for productive microorganisms. In this study, we doubled the gene copy for EGT synthesis and deleted the histidine ammonia-lyase gene in a potent EGT-producing methylotrophic bacterium Methylobacterium aquaticum strain 22A, and optimized its culture conditions, resulting in increased EGT production of 7.0 mg EGT/g dry cell weight and 100 μg EGT/5 mL/7 days. In addition, through screening we found EGT-producing eukaryotic strains of Aureobasidium pullulans and Rhodotorula mucilaginosa, which can produce 1.0 and 3.2 mg EGT/g dry cell weight, 70 and 120 μg EGT/5 mL/7 days, respectively. This study proposes practical uses of potent EGT-producing recombinant Methylobacterium species and non-recombinant yeast and fungal strains.</description><subject>Animals</subject><subject>Anti-oxidative amino acid</subject><subject>Antioxidants - metabolism</subject><subject>Aureobasidium pullulans</subject><subject>Ergothioneine</subject><subject>Ergothioneine - biosynthesis</subject><subject>Fungi - genetics</subject><subject>Fungi - metabolism</subject><subject>Histidine - metabolism</subject><subject>Humans</subject><subject>Metabolic Engineering</subject><subject>Methanol</subject><subject>Methanol - metabolism</subject><subject>Methylobacterium - genetics</subject><subject>Methylobacterium - metabolism</subject><subject>Methylobacterium species</subject><subject>Organisms, Genetically Modified</subject><subject>Oxidation-Reduction</subject><subject>Rhodotorula - genetics</subject><subject>Rhodotorula - growth & development</subject><subject>Rhodotorula - metabolism</subject><subject>Rhodotorula mucilaginosa</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Yeasts - genetics</subject><subject>Yeasts - metabolism</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhi0EoqXwDxDKyNAE24lje0FCVYFKRSwwW45zaR2lSbETpP57XKUwMt2H3rv37kHoluCEYJI_1Eld2M6bhGIiEswSTMkZmpI043GWUXJ-zIWMCafpBF15X2NMOObkEk2olGGHkFO0WrpN129t14JtIdq7rhxMH8po8LbdRG_Qbw9NV2jTg7PDLvJ7MBb8PDqA9v080m0ZVUO7sdfootKNh5tTnKHP5-XH4jVev7-sFk_r2DBM-jgXMktLIWnBSJUyInjKTVrgouIypxUWJE8h9GWuaaZNKQzRmcCUlzzLcZGnM3Q_7g23fg3ge7Wz3kDT6Ba6wSuKWc6ZFIwFaTZKjeu8d1CpvbM77Q6KYHWEqGo1QlRHiAozFSCGsbuTw1DsoPwb-qUWBI-jAMKf3xac8oFJa6C0Dkyvys7-7_ADjSqD1Q</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Fujitani, Yoshiko</creator><creator>Alamgir, Kabir Md</creator><creator>Tani, Akio</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201812</creationdate><title>Ergothioneine production using Methylobacterium species, yeast, and fungi</title><author>Fujitani, Yoshiko ; Alamgir, Kabir Md ; Tani, Akio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-68943d892b51f3518737c3b0bf7962f08163e35196a24acd8c1a48027d7460b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Anti-oxidative amino acid</topic><topic>Antioxidants - metabolism</topic><topic>Aureobasidium pullulans</topic><topic>Ergothioneine</topic><topic>Ergothioneine - biosynthesis</topic><topic>Fungi - genetics</topic><topic>Fungi - metabolism</topic><topic>Histidine - metabolism</topic><topic>Humans</topic><topic>Metabolic Engineering</topic><topic>Methanol</topic><topic>Methanol - metabolism</topic><topic>Methylobacterium - genetics</topic><topic>Methylobacterium - metabolism</topic><topic>Methylobacterium species</topic><topic>Organisms, Genetically Modified</topic><topic>Oxidation-Reduction</topic><topic>Rhodotorula - genetics</topic><topic>Rhodotorula - growth & development</topic><topic>Rhodotorula - metabolism</topic><topic>Rhodotorula mucilaginosa</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Yeasts - genetics</topic><topic>Yeasts - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujitani, Yoshiko</creatorcontrib><creatorcontrib>Alamgir, Kabir Md</creatorcontrib><creatorcontrib>Tani, Akio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujitani, Yoshiko</au><au>Alamgir, Kabir Md</au><au>Tani, Akio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ergothioneine production using Methylobacterium species, yeast, and fungi</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2018-12</date><risdate>2018</risdate><volume>126</volume><issue>6</issue><spage>715</spage><epage>722</epage><pages>715-722</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>Ergothioneine (EGT) is a sulfur-containing, anti-oxidative amino acid derived from histidine. EGT is synthesized in bacteria and fungi but not in animals and plants, and is now recognized as important for human health. Its cost-effective fermentative production has not been elucidated due to the lack of information for productive microorganisms. In this study, we doubled the gene copy for EGT synthesis and deleted the histidine ammonia-lyase gene in a potent EGT-producing methylotrophic bacterium Methylobacterium aquaticum strain 22A, and optimized its culture conditions, resulting in increased EGT production of 7.0 mg EGT/g dry cell weight and 100 μg EGT/5 mL/7 days. In addition, through screening we found EGT-producing eukaryotic strains of Aureobasidium pullulans and Rhodotorula mucilaginosa, which can produce 1.0 and 3.2 mg EGT/g dry cell weight, 70 and 120 μg EGT/5 mL/7 days, respectively. This study proposes practical uses of potent EGT-producing recombinant Methylobacterium species and non-recombinant yeast and fungal strains.</abstract><cop>Japan</cop><pub>Elsevier B.V</pub><pmid>29910189</pmid><doi>10.1016/j.jbiosc.2018.05.021</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1389-1723 |
| ispartof | Journal of bioscience and bioengineering, 2018-12, Vol.126 (6), p.715-722 |
| issn | 1389-1723 1347-4421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2056759855 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Anti-oxidative amino acid Antioxidants - metabolism Aureobasidium pullulans Ergothioneine Ergothioneine - biosynthesis Fungi - genetics Fungi - metabolism Histidine - metabolism Humans Metabolic Engineering Methanol Methanol - metabolism Methylobacterium - genetics Methylobacterium - metabolism Methylobacterium species Organisms, Genetically Modified Oxidation-Reduction Rhodotorula - genetics Rhodotorula - growth & development Rhodotorula - metabolism Rhodotorula mucilaginosa Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Yeasts - genetics Yeasts - metabolism |
| title | Ergothioneine production using Methylobacterium species, yeast, and fungi |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T19%3A00%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ergothioneine%20production%20using%20Methylobacterium%20species,%20yeast,%20and%20fungi&rft.jtitle=Journal%20of%20bioscience%20and%20bioengineering&rft.au=Fujitani,%20Yoshiko&rft.date=2018-12&rft.volume=126&rft.issue=6&rft.spage=715&rft.epage=722&rft.pages=715-722&rft.issn=1389-1723&rft.eissn=1347-4421&rft_id=info:doi/10.1016/j.jbiosc.2018.05.021&rft_dat=%3Cproquest_cross%3E2056759855%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2056759855&rft_id=info:pmid/29910189&rft_els_id=S1389172318302950&rfr_iscdi=true |