Detection of human influenza A (H1N1) and B strains with reduced sensitivity to neuraminidase inhibitors
Abstract Background Neuraminidase inhibitors (NAIs) have been used since 2005 in France. Objective Influenza viruses isolated in hospital and community cases in winter 2005–2006 were evaluated for their sensitivity to NAIs. Study design Isolates were tested in duplicate with a fluorescence-based neu...
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| Veröffentlicht in: | Journal of clinical virology 2008-01, Vol.41 (1), p.25-28 |
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| creator | Escuret, Vanessa Frobert, Emilie Bouscambert-Duchamp, Maude Sabatier, Murielle Grog, Isidore Valette, Martine Lina, Bruno Morfin, Florence Ferraris, Olivier |
| description | Abstract Background Neuraminidase inhibitors (NAIs) have been used since 2005 in France. Objective Influenza viruses isolated in hospital and community cases in winter 2005–2006 were evaluated for their sensitivity to NAIs. Study design Isolates were tested in duplicate with a fluorescence-based neuraminidase assay. The IC50 for oseltamivir or zanamivir was calculated for each strain. Mean IC50 (±S.D.) are expressed in nM. Viruses with IC50 superior or very superior to the upper limit (mean IC50 + 2.5 S.D.) were considered as outliers or resistant viruses, respectively. HA and NA genes for outliers, resistant strains and for a few sensitive strains were sequenced. Results Out of 225 B isolates, one was found resistant to both oseltamivir and zanamivir with a D197Y mutation in NA and eight isolates were outliers for oseltamivir and/or zanamivir. Out of 151 A (H1N1) isolates, one was found resistant to oseltamivir but sensitive to zanamivir with a H275Y mutation in NA, two isolates were resistant to zanamivir and three isolates were outliers for oseltamivir and/or zanamivir. New mutations were detected in outliers compared to sensitive viruses. Conclusion Resistant influenza strains to NAIs are circulating at a stable and low level of 1% since the introduction of NAIs in clinical practice. |
| doi_str_mv | 10.1016/j.jcv.2007.10.019 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_20565468</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1386653207003897</els_id><sourcerecordid>20565468</sourcerecordid><originalsourceid>FETCH-LOGICAL-c437t-8d2b74f12aebcde87d9163e62885d6a33edb84b391ea5a2e29422d2d7a2caf4b3</originalsourceid><addsrcrecordid>eNp9kUuLFDEUhQtRnIf-ADeSlYyLavOoVFIIwjzUEQZdqOuQSm7RKauSMUm1tL_eFN0wMAtXSW7OOXC_U1WvCN4QTNp342Y0uw3FWJT3BpPuSXVKpGA171rxtNyZbOuWM3pSnaU0Ykw4a8Tz6oRIzDnl7LTa3kAGk13wKAxou8zaI-eHaQH_V6NLdHFLvpK3SHuLrlDKUTuf0B-XtyiCXQxYlMAnl93O5T3KAXlYop6dd1YnKFFb17scYnpRPRv0lODl8Tyvfn76-OP6tr779vnL9eVdbRomci0t7UUzEKqhNxaksB1pGbRUSm5bzRjYXjY96whorinQrqHUUis0NXooH-fVm0PufQy_F0hZzS4ZmCbtISxJUcxb3rSyCMlBaGJIKcKg7qObddwrgtWKV42q4FUr3nVU8BbP62P40s9gHxxHnkXw_iCAsuLOQVTJOPCFk4sFs7LB_Tf-wyO3mQpJo6dfsIc0hiX6wk4RlajC6vva71ovFhgz2Qn2D2LFoK0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20565468</pqid></control><display><type>article</type><title>Detection of human influenza A (H1N1) and B strains with reduced sensitivity to neuraminidase inhibitors</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Escuret, Vanessa ; Frobert, Emilie ; Bouscambert-Duchamp, Maude ; Sabatier, Murielle ; Grog, Isidore ; Valette, Martine ; Lina, Bruno ; Morfin, Florence ; Ferraris, Olivier</creator><creatorcontrib>Escuret, Vanessa ; Frobert, Emilie ; Bouscambert-Duchamp, Maude ; Sabatier, Murielle ; Grog, Isidore ; Valette, Martine ; Lina, Bruno ; Morfin, Florence ; Ferraris, Olivier</creatorcontrib><description>Abstract Background Neuraminidase inhibitors (NAIs) have been used since 2005 in France. Objective Influenza viruses isolated in hospital and community cases in winter 2005–2006 were evaluated for their sensitivity to NAIs. Study design Isolates were tested in duplicate with a fluorescence-based neuraminidase assay. The IC50 for oseltamivir or zanamivir was calculated for each strain. Mean IC50 (±S.D.) are expressed in nM. Viruses with IC50 superior or very superior to the upper limit (mean IC50 + 2.5 S.D.) were considered as outliers or resistant viruses, respectively. HA and NA genes for outliers, resistant strains and for a few sensitive strains were sequenced. Results Out of 225 B isolates, one was found resistant to both oseltamivir and zanamivir with a D197Y mutation in NA and eight isolates were outliers for oseltamivir and/or zanamivir. Out of 151 A (H1N1) isolates, one was found resistant to oseltamivir but sensitive to zanamivir with a H275Y mutation in NA, two isolates were resistant to zanamivir and three isolates were outliers for oseltamivir and/or zanamivir. New mutations were detected in outliers compared to sensitive viruses. Conclusion Resistant influenza strains to NAIs are circulating at a stable and low level of 1% since the introduction of NAIs in clinical practice.</description><identifier>ISSN: 1386-6532</identifier><identifier>EISSN: 1873-5967</identifier><identifier>DOI: 10.1016/j.jcv.2007.10.019</identifier><identifier>PMID: 18055253</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Allergy and Immunology ; Amino Acid Substitution ; Antiviral Agents - pharmacology ; Drug Resistance, Viral ; France ; Humans ; Infectious Disease ; Influenza ; Influenza A Virus, H1N1 Subtype - drug effects ; Influenza A Virus, H1N1 Subtype - genetics ; Influenza A Virus, H1N1 Subtype - isolation & purification ; Influenza B virus - drug effects ; Influenza B virus - isolation & purification ; Influenza, Human - virology ; Inhibitory Concentration 50 ; Microbial Sensitivity Tests ; Mutation, Missense ; Nais ; Neuraminidase - genetics ; Neuraminidase inhibitors ; Oseltamivir - pharmacology ; Resistance ; Sequence Analysis, DNA ; Systematic monitoring ; Viral Proteins - genetics ; Zanamivir - pharmacology</subject><ispartof>Journal of clinical virology, 2008-01, Vol.41 (1), p.25-28</ispartof><rights>Elsevier B.V.</rights><rights>2007 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-8d2b74f12aebcde87d9163e62885d6a33edb84b391ea5a2e29422d2d7a2caf4b3</citedby><cites>FETCH-LOGICAL-c437t-8d2b74f12aebcde87d9163e62885d6a33edb84b391ea5a2e29422d2d7a2caf4b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jcv.2007.10.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18055253$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Escuret, Vanessa</creatorcontrib><creatorcontrib>Frobert, Emilie</creatorcontrib><creatorcontrib>Bouscambert-Duchamp, Maude</creatorcontrib><creatorcontrib>Sabatier, Murielle</creatorcontrib><creatorcontrib>Grog, Isidore</creatorcontrib><creatorcontrib>Valette, Martine</creatorcontrib><creatorcontrib>Lina, Bruno</creatorcontrib><creatorcontrib>Morfin, Florence</creatorcontrib><creatorcontrib>Ferraris, Olivier</creatorcontrib><title>Detection of human influenza A (H1N1) and B strains with reduced sensitivity to neuraminidase inhibitors</title><title>Journal of clinical virology</title><addtitle>J Clin Virol</addtitle><description>Abstract Background Neuraminidase inhibitors (NAIs) have been used since 2005 in France. Objective Influenza viruses isolated in hospital and community cases in winter 2005–2006 were evaluated for their sensitivity to NAIs. Study design Isolates were tested in duplicate with a fluorescence-based neuraminidase assay. The IC50 for oseltamivir or zanamivir was calculated for each strain. Mean IC50 (±S.D.) are expressed in nM. Viruses with IC50 superior or very superior to the upper limit (mean IC50 + 2.5 S.D.) were considered as outliers or resistant viruses, respectively. HA and NA genes for outliers, resistant strains and for a few sensitive strains were sequenced. Results Out of 225 B isolates, one was found resistant to both oseltamivir and zanamivir with a D197Y mutation in NA and eight isolates were outliers for oseltamivir and/or zanamivir. Out of 151 A (H1N1) isolates, one was found resistant to oseltamivir but sensitive to zanamivir with a H275Y mutation in NA, two isolates were resistant to zanamivir and three isolates were outliers for oseltamivir and/or zanamivir. New mutations were detected in outliers compared to sensitive viruses. Conclusion Resistant influenza strains to NAIs are circulating at a stable and low level of 1% since the introduction of NAIs in clinical practice.</description><subject>Allergy and Immunology</subject><subject>Amino Acid Substitution</subject><subject>Antiviral Agents - pharmacology</subject><subject>Drug Resistance, Viral</subject><subject>France</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Influenza</subject><subject>Influenza A Virus, H1N1 Subtype - drug effects</subject><subject>Influenza A Virus, H1N1 Subtype - genetics</subject><subject>Influenza A Virus, H1N1 Subtype - isolation & purification</subject><subject>Influenza B virus - drug effects</subject><subject>Influenza B virus - isolation & purification</subject><subject>Influenza, Human - virology</subject><subject>Inhibitory Concentration 50</subject><subject>Microbial Sensitivity Tests</subject><subject>Mutation, Missense</subject><subject>Nais</subject><subject>Neuraminidase - genetics</subject><subject>Neuraminidase inhibitors</subject><subject>Oseltamivir - pharmacology</subject><subject>Resistance</subject><subject>Sequence Analysis, DNA</subject><subject>Systematic monitoring</subject><subject>Viral Proteins - genetics</subject><subject>Zanamivir - pharmacology</subject><issn>1386-6532</issn><issn>1873-5967</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUuLFDEUhQtRnIf-ADeSlYyLavOoVFIIwjzUEQZdqOuQSm7RKauSMUm1tL_eFN0wMAtXSW7OOXC_U1WvCN4QTNp342Y0uw3FWJT3BpPuSXVKpGA171rxtNyZbOuWM3pSnaU0Ykw4a8Tz6oRIzDnl7LTa3kAGk13wKAxou8zaI-eHaQH_V6NLdHFLvpK3SHuLrlDKUTuf0B-XtyiCXQxYlMAnl93O5T3KAXlYop6dd1YnKFFb17scYnpRPRv0lODl8Tyvfn76-OP6tr779vnL9eVdbRomci0t7UUzEKqhNxaksB1pGbRUSm5bzRjYXjY96whorinQrqHUUis0NXooH-fVm0PufQy_F0hZzS4ZmCbtISxJUcxb3rSyCMlBaGJIKcKg7qObddwrgtWKV42q4FUr3nVU8BbP62P40s9gHxxHnkXw_iCAsuLOQVTJOPCFk4sFs7LB_Tf-wyO3mQpJo6dfsIc0hiX6wk4RlajC6vva71ovFhgz2Qn2D2LFoK0</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Escuret, Vanessa</creator><creator>Frobert, Emilie</creator><creator>Bouscambert-Duchamp, Maude</creator><creator>Sabatier, Murielle</creator><creator>Grog, Isidore</creator><creator>Valette, Martine</creator><creator>Lina, Bruno</creator><creator>Morfin, Florence</creator><creator>Ferraris, Olivier</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20080101</creationdate><title>Detection of human influenza A (H1N1) and B strains with reduced sensitivity to neuraminidase inhibitors</title><author>Escuret, Vanessa ; Frobert, Emilie ; Bouscambert-Duchamp, Maude ; Sabatier, Murielle ; Grog, Isidore ; Valette, Martine ; Lina, Bruno ; Morfin, Florence ; Ferraris, Olivier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-8d2b74f12aebcde87d9163e62885d6a33edb84b391ea5a2e29422d2d7a2caf4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Allergy and Immunology</topic><topic>Amino Acid Substitution</topic><topic>Antiviral Agents - pharmacology</topic><topic>Drug Resistance, Viral</topic><topic>France</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Influenza</topic><topic>Influenza A Virus, H1N1 Subtype - drug effects</topic><topic>Influenza A Virus, H1N1 Subtype - genetics</topic><topic>Influenza A Virus, H1N1 Subtype - isolation & purification</topic><topic>Influenza B virus - drug effects</topic><topic>Influenza B virus - isolation & purification</topic><topic>Influenza, Human - virology</topic><topic>Inhibitory Concentration 50</topic><topic>Microbial Sensitivity Tests</topic><topic>Mutation, Missense</topic><topic>Nais</topic><topic>Neuraminidase - genetics</topic><topic>Neuraminidase inhibitors</topic><topic>Oseltamivir - pharmacology</topic><topic>Resistance</topic><topic>Sequence Analysis, DNA</topic><topic>Systematic monitoring</topic><topic>Viral Proteins - genetics</topic><topic>Zanamivir - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Escuret, Vanessa</creatorcontrib><creatorcontrib>Frobert, Emilie</creatorcontrib><creatorcontrib>Bouscambert-Duchamp, Maude</creatorcontrib><creatorcontrib>Sabatier, Murielle</creatorcontrib><creatorcontrib>Grog, Isidore</creatorcontrib><creatorcontrib>Valette, Martine</creatorcontrib><creatorcontrib>Lina, Bruno</creatorcontrib><creatorcontrib>Morfin, Florence</creatorcontrib><creatorcontrib>Ferraris, Olivier</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of clinical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Escuret, Vanessa</au><au>Frobert, Emilie</au><au>Bouscambert-Duchamp, Maude</au><au>Sabatier, Murielle</au><au>Grog, Isidore</au><au>Valette, Martine</au><au>Lina, Bruno</au><au>Morfin, Florence</au><au>Ferraris, Olivier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of human influenza A (H1N1) and B strains with reduced sensitivity to neuraminidase inhibitors</atitle><jtitle>Journal of clinical virology</jtitle><addtitle>J Clin Virol</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>41</volume><issue>1</issue><spage>25</spage><epage>28</epage><pages>25-28</pages><issn>1386-6532</issn><eissn>1873-5967</eissn><abstract>Abstract Background Neuraminidase inhibitors (NAIs) have been used since 2005 in France. Objective Influenza viruses isolated in hospital and community cases in winter 2005–2006 were evaluated for their sensitivity to NAIs. Study design Isolates were tested in duplicate with a fluorescence-based neuraminidase assay. The IC50 for oseltamivir or zanamivir was calculated for each strain. Mean IC50 (±S.D.) are expressed in nM. Viruses with IC50 superior or very superior to the upper limit (mean IC50 + 2.5 S.D.) were considered as outliers or resistant viruses, respectively. HA and NA genes for outliers, resistant strains and for a few sensitive strains were sequenced. Results Out of 225 B isolates, one was found resistant to both oseltamivir and zanamivir with a D197Y mutation in NA and eight isolates were outliers for oseltamivir and/or zanamivir. Out of 151 A (H1N1) isolates, one was found resistant to oseltamivir but sensitive to zanamivir with a H275Y mutation in NA, two isolates were resistant to zanamivir and three isolates were outliers for oseltamivir and/or zanamivir. New mutations were detected in outliers compared to sensitive viruses. Conclusion Resistant influenza strains to NAIs are circulating at a stable and low level of 1% since the introduction of NAIs in clinical practice.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>18055253</pmid><doi>10.1016/j.jcv.2007.10.019</doi><tpages>4</tpages></addata></record> |
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| subjects | Allergy and Immunology Amino Acid Substitution Antiviral Agents - pharmacology Drug Resistance, Viral France Humans Infectious Disease Influenza Influenza A Virus, H1N1 Subtype - drug effects Influenza A Virus, H1N1 Subtype - genetics Influenza A Virus, H1N1 Subtype - isolation & purification Influenza B virus - drug effects Influenza B virus - isolation & purification Influenza, Human - virology Inhibitory Concentration 50 Microbial Sensitivity Tests Mutation, Missense Nais Neuraminidase - genetics Neuraminidase inhibitors Oseltamivir - pharmacology Resistance Sequence Analysis, DNA Systematic monitoring Viral Proteins - genetics Zanamivir - pharmacology |
| title | Detection of human influenza A (H1N1) and B strains with reduced sensitivity to neuraminidase inhibitors |
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