Paired box 7 inhibits differentiation in 3T3‐L1 preadipocytes
Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD...
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| Veröffentlicht in: | Animal science journal 2018-08, Vol.89 (8), p.1214-1219 |
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| creator | Izumi, Wakana Takuma, Yuko Ebihara, Ryo Mizunoya, Wataru Tatsumi, Ryuichi Nakamura, Mako |
| description | Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in nonmyogenic lineage cells. In this study, we performed experiments using stably expressed Pax7 in 3T3‐L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. We found that Pax7 represses adipogenic markers and prevents differentiation. These cells showed decreased expression of PDGFRα, PPARγ and Fabp4 and inhibited forming lipid droplets. |
| doi_str_mv | 10.1111/asj.13050 |
| format | Article |
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Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in nonmyogenic lineage cells. In this study, we performed experiments using stably expressed Pax7 in 3T3‐L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. We found that Pax7 represses adipogenic markers and prevents differentiation. These cells showed decreased expression of PDGFRα, PPARγ and Fabp4 and inhibited forming lipid droplets.</description><identifier>ISSN: 1344-3941</identifier><identifier>EISSN: 1740-0929</identifier><identifier>DOI: 10.1111/asj.13050</identifier><identifier>PMID: 29900636</identifier><language>eng</language><publisher>Australia: Blackwell Publishing Ltd</publisher><subject>3T3-L1 Cells ; 3T3‐L1 ; adipocyte differentiation ; Adipocytes - physiology ; Adipogenesis ; Adipogenesis - genetics ; Animals ; Biomarkers ; Cell Differentiation - genetics ; Differentiation ; Down-Regulation - genetics ; Fatty Acid-Binding Proteins - genetics ; Fatty Acid-Binding Proteins - metabolism ; Gene Expression - genetics ; Lipid Droplets ; Mice ; Muscle Development ; Muscles ; MyoD protein ; MyoD Protein - physiology ; Myogenesis ; Myogenic Regulatory Factor 5 - physiology ; Pax7 ; PAX7 Transcription Factor - physiology ; PDGFRα ; PPAR gamma - genetics ; PPAR gamma - metabolism ; PPARγ ; Preadipocytes ; Receptor, Platelet-Derived Growth Factor alpha - genetics ; Stem cells</subject><ispartof>Animal science journal, 2018-08, Vol.89 (8), p.1214-1219</ispartof><rights>2018 Japanese Society of Animal Science</rights><rights>2018 Japanese Society of Animal Science.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4430-7da2da9de1e04122273fba450a674704ee70455855d381854144849c81ec52703</citedby><cites>FETCH-LOGICAL-c4430-7da2da9de1e04122273fba450a674704ee70455855d381854144849c81ec52703</cites><orcidid>0000-0002-6129-7560</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fasj.13050$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fasj.13050$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29900636$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Izumi, Wakana</creatorcontrib><creatorcontrib>Takuma, Yuko</creatorcontrib><creatorcontrib>Ebihara, Ryo</creatorcontrib><creatorcontrib>Mizunoya, Wataru</creatorcontrib><creatorcontrib>Tatsumi, Ryuichi</creatorcontrib><creatorcontrib>Nakamura, Mako</creatorcontrib><title>Paired box 7 inhibits differentiation in 3T3‐L1 preadipocytes</title><title>Animal science journal</title><addtitle>Anim Sci J</addtitle><description>Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in nonmyogenic lineage cells. In this study, we performed experiments using stably expressed Pax7 in 3T3‐L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. We found that Pax7 represses adipogenic markers and prevents differentiation. These cells showed decreased expression of PDGFRα, PPARγ and Fabp4 and inhibited forming lipid droplets.</description><subject>3T3-L1 Cells</subject><subject>3T3‐L1</subject><subject>adipocyte differentiation</subject><subject>Adipocytes - physiology</subject><subject>Adipogenesis</subject><subject>Adipogenesis - genetics</subject><subject>Animals</subject><subject>Biomarkers</subject><subject>Cell Differentiation - genetics</subject><subject>Differentiation</subject><subject>Down-Regulation - genetics</subject><subject>Fatty Acid-Binding Proteins - genetics</subject><subject>Fatty Acid-Binding Proteins - metabolism</subject><subject>Gene Expression - genetics</subject><subject>Lipid Droplets</subject><subject>Mice</subject><subject>Muscle Development</subject><subject>Muscles</subject><subject>MyoD protein</subject><subject>MyoD Protein - physiology</subject><subject>Myogenesis</subject><subject>Myogenic Regulatory Factor 5 - physiology</subject><subject>Pax7</subject><subject>PAX7 Transcription Factor - physiology</subject><subject>PDGFRα</subject><subject>PPAR gamma - genetics</subject><subject>PPAR gamma - metabolism</subject><subject>PPARγ</subject><subject>Preadipocytes</subject><subject>Receptor, Platelet-Derived Growth Factor alpha - genetics</subject><subject>Stem cells</subject><issn>1344-3941</issn><issn>1740-0929</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10E9LwzAYBvAgipvTg19ACl700O1Nk7TNScbwLwMF57mk7VvM6NqatOhufgQ_o5_EzE4Pgjkkgfx4ePMQckxhTN2aKLscUwYCdsiQRhx8kIHcdXfGuc8kpwNyYO0SgEYSxD4ZBFIChCwckosHpQ3mXlq_eZGnq2ed6tZ6uS4KNFi1WrW6rtyDxxbs8_1jTr3GoMp1U2frFu0h2StUafFoe47I09XlYnbjz--vb2fTuZ9xzsCPchXkSuZIETgNgiBiRaq4ABVGPAKO6DYhYiFyFtNYcMp5zGUWU8xEEAEbkbM-tzH1S4e2TVbaZliWqsK6s0kAQoTUfXtDT__QZd2Zyk3nVMyElCKMnTrvVWZqaw0WSWP0Spl1QiHZtJq4VpPvVp092SZ26QrzX_lTowOTHrzqEtf_JyXTx7s-8gvnan3S</recordid><startdate>201808</startdate><enddate>201808</enddate><creator>Izumi, Wakana</creator><creator>Takuma, Yuko</creator><creator>Ebihara, Ryo</creator><creator>Mizunoya, Wataru</creator><creator>Tatsumi, Ryuichi</creator><creator>Nakamura, Mako</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6129-7560</orcidid></search><sort><creationdate>201808</creationdate><title>Paired box 7 inhibits differentiation in 3T3‐L1 preadipocytes</title><author>Izumi, Wakana ; Takuma, Yuko ; Ebihara, Ryo ; Mizunoya, Wataru ; Tatsumi, Ryuichi ; Nakamura, Mako</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4430-7da2da9de1e04122273fba450a674704ee70455855d381854144849c81ec52703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>3T3-L1 Cells</topic><topic>3T3‐L1</topic><topic>adipocyte differentiation</topic><topic>Adipocytes - physiology</topic><topic>Adipogenesis</topic><topic>Adipogenesis - genetics</topic><topic>Animals</topic><topic>Biomarkers</topic><topic>Cell Differentiation - genetics</topic><topic>Differentiation</topic><topic>Down-Regulation - genetics</topic><topic>Fatty Acid-Binding Proteins - genetics</topic><topic>Fatty Acid-Binding Proteins - metabolism</topic><topic>Gene Expression - genetics</topic><topic>Lipid Droplets</topic><topic>Mice</topic><topic>Muscle Development</topic><topic>Muscles</topic><topic>MyoD protein</topic><topic>MyoD Protein - physiology</topic><topic>Myogenesis</topic><topic>Myogenic Regulatory Factor 5 - physiology</topic><topic>Pax7</topic><topic>PAX7 Transcription Factor - physiology</topic><topic>PDGFRα</topic><topic>PPAR gamma - genetics</topic><topic>PPAR gamma - metabolism</topic><topic>PPARγ</topic><topic>Preadipocytes</topic><topic>Receptor, Platelet-Derived Growth Factor alpha - genetics</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Izumi, Wakana</creatorcontrib><creatorcontrib>Takuma, Yuko</creatorcontrib><creatorcontrib>Ebihara, Ryo</creatorcontrib><creatorcontrib>Mizunoya, Wataru</creatorcontrib><creatorcontrib>Tatsumi, Ryuichi</creatorcontrib><creatorcontrib>Nakamura, Mako</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Animal science journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Izumi, Wakana</au><au>Takuma, Yuko</au><au>Ebihara, Ryo</au><au>Mizunoya, Wataru</au><au>Tatsumi, Ryuichi</au><au>Nakamura, Mako</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Paired box 7 inhibits differentiation in 3T3‐L1 preadipocytes</atitle><jtitle>Animal science journal</jtitle><addtitle>Anim Sci J</addtitle><date>2018-08</date><risdate>2018</risdate><volume>89</volume><issue>8</issue><spage>1214</spage><epage>1219</epage><pages>1214-1219</pages><issn>1344-3941</issn><eissn>1740-0929</eissn><abstract>Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in nonmyogenic lineage cells. In this study, we performed experiments using stably expressed Pax7 in 3T3‐L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. We found that Pax7 represses adipogenic markers and prevents differentiation. These cells showed decreased expression of PDGFRα, PPARγ and Fabp4 and inhibited forming lipid droplets.</abstract><cop>Australia</cop><pub>Blackwell Publishing Ltd</pub><pmid>29900636</pmid><doi>10.1111/asj.13050</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-6129-7560</orcidid></addata></record> |
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| subjects | 3T3-L1 Cells 3T3‐L1 adipocyte differentiation Adipocytes - physiology Adipogenesis Adipogenesis - genetics Animals Biomarkers Cell Differentiation - genetics Differentiation Down-Regulation - genetics Fatty Acid-Binding Proteins - genetics Fatty Acid-Binding Proteins - metabolism Gene Expression - genetics Lipid Droplets Mice Muscle Development Muscles MyoD protein MyoD Protein - physiology Myogenesis Myogenic Regulatory Factor 5 - physiology Pax7 PAX7 Transcription Factor - physiology PDGFRα PPAR gamma - genetics PPAR gamma - metabolism PPARγ Preadipocytes Receptor, Platelet-Derived Growth Factor alpha - genetics Stem cells |
| title | Paired box 7 inhibits differentiation in 3T3‐L1 preadipocytes |
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