Reverse transcription loop-mediated isothermal amplification (RT-LAMP) designed for fast and sensitive on-site detection of Pepper chat fruit viroid (PCFVd)

•A fast, sensitive and robust one step RT-LAMP was developed for PCFVd detection.•The RT-LAMP reaction can be completed within 40 min.•Sensitivity of the LAMP assay was up to 100x higher than conventional RT-PCR assays.•Simple lysis procedure and SYTO9 potentially facilitates PCFVd on-site detection...

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Bibliographische Detailangaben

Veröffentlicht in:	Journal of virological methods 2018-09, Vol.259, p.81-91
Hauptverfasser:	Tangkanchanapas, Parichate, Höfte, Monica, De Jonghe, Kris
Format:	Artikel
Sprache:	eng
Schlagworte:	LAMP Point-of-care diagnostics Pospiviroids
Online-Zugang:	Volltext
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