In situ 3D magnetic resonance metabolic imaging of microwave-irradiated rodent brain: a new tool for metabolomics research
The rapid elevation in rat brain temperature achieveable with focused beam microwave irradiation (FBMI) leads to a permanent inactivation of enzymes, thereby minimizing enzyme-dependent post-mortem metabolic changes. An additional characteristic of FBMI is that the NMR properties of the tissue are c...
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| Veröffentlicht in: | Journal of neurochemistry 2009-04, Vol.109 (2), p.494-501 |
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| creator | de Graaf, Robin A Chowdhury, Golam M.I Brown, Peter B Rothman, Douglas L Behar, Kevin L |
| description | The rapid elevation in rat brain temperature achieveable with focused beam microwave irradiation (FBMI) leads to a permanent inactivation of enzymes, thereby minimizing enzyme-dependent post-mortem metabolic changes. An additional characteristic of FBMI is that the NMR properties of the tissue are close to those of the in vivo condition and remain so for at least 12 h. These features create an opportunity to develop magnetic resonance spectroscopy and imaging on microwave-irradiated samples into a technique with a resolution, coverage and sensitivity superior to any experiment performed directly in vivo. Furthermore, when combined with pre-FBMI infusion of ¹³C-labeled substrates, like [1-¹³C]-glucose, the technique can generate maps of metabolic fluxes, like the tricarboxylic acid and glutamate-glutamine neurotransmitter cycle fluxes at an unprecedented spatial resolution. |
| doi_str_mv | 10.1111/j.1471-4159.2009.05967.x |
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An additional characteristic of FBMI is that the NMR properties of the tissue are close to those of the in vivo condition and remain so for at least 12 h. These features create an opportunity to develop magnetic resonance spectroscopy and imaging on microwave-irradiated samples into a technique with a resolution, coverage and sensitivity superior to any experiment performed directly in vivo. Furthermore, when combined with pre-FBMI infusion of ¹³C-labeled substrates, like [1-¹³C]-glucose, the technique can generate maps of metabolic fluxes, like the tricarboxylic acid and glutamate-glutamine neurotransmitter cycle fluxes at an unprecedented spatial resolution.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain - diagnostic imaging</subject><subject>Brain - metabolism</subject><subject>Brain - radiation effects</subject><subject>Central nervous system</subject><subject>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Imaging, Three-Dimensional - methods</subject><subject>Imaging, Three-Dimensional - trends</subject><subject>in situ NMR spectroscopy</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>Male</subject><subject>Metabolomics - methods</subject><subject>Metabolomics - trends</subject><subject>microwave fixation</subject><subject>Microwaves</subject><subject>Neurology</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Radionuclide Imaging</subject><subject>rat brain</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rodents</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkktv1DAUhSMEotPCXwALie4Srh-JEyQWaHgVVbCArq0bxx48ytjFTpiWX4_DDEVihTe2fL9zfHWPi4JQqGheL7YVFZKWgtZdxQC6CuqukdXNvWJ1V7hfrAAYKzkIdlKcprQFoI1o6MPihHZZxXmzKn5eeJLcNBP-huxw483kNIkmBY9eG7IzE_ZhzHcuV53fkGDJzukY9vjDlC5GHBxOZiAxDMZPpI_o_EuCxJs9mUIYiQ3xj03IyrS4G4z626PigcUxmcfH_ay4evf26_pDefn5_cX69WWp666WpeBDa7GzMHRSdCCQUjAWje56lE3NNYNGSGgk6F5IbHvktm1l03POBec9PyvOD77XMXyfTZrUziVtxhG9CXNSDGpRC95m8Nk_4DbM0efeMtPUtGXAMtQeoDyDlKKx6jrm2cRbRUEt4aitWjJQSwZqCUf9DkfdZOmTo__c78zwV3hMIwPPjwAmjaONOQOX7jhGWculXLhXB27vRnP73w2oj5_Wyynrnx70FoPCTcxvXH1hQHn-ICBrBvwXd76zeA</recordid><startdate>200904</startdate><enddate>200904</enddate><creator>de Graaf, Robin A</creator><creator>Chowdhury, Golam M.I</creator><creator>Brown, Peter B</creator><creator>Rothman, Douglas L</creator><creator>Behar, Kevin L</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>200904</creationdate><title>In situ 3D magnetic resonance metabolic imaging of microwave-irradiated rodent brain: a new tool for metabolomics research</title><author>de Graaf, Robin A ; Chowdhury, Golam M.I ; Brown, Peter B ; Rothman, Douglas L ; Behar, Kevin L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5957-43d8fa9f0d974904a110efaec9ba7653c206470670cb47a8ba3f8876b333433b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain - diagnostic imaging</topic><topic>Brain - metabolism</topic><topic>Brain - radiation effects</topic><topic>Central nervous system</topic><topic>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</topic><topic>Fundamental and applied biological sciences. 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An additional characteristic of FBMI is that the NMR properties of the tissue are close to those of the in vivo condition and remain so for at least 12 h. These features create an opportunity to develop magnetic resonance spectroscopy and imaging on microwave-irradiated samples into a technique with a resolution, coverage and sensitivity superior to any experiment performed directly in vivo. Furthermore, when combined with pre-FBMI infusion of ¹³C-labeled substrates, like [1-¹³C]-glucose, the technique can generate maps of metabolic fluxes, like the tricarboxylic acid and glutamate-glutamine neurotransmitter cycle fluxes at an unprecedented spatial resolution.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19200336</pmid><doi>10.1111/j.1471-4159.2009.05967.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biochemistry Biochemistry and metabolism Biological and medical sciences Brain - diagnostic imaging Brain - metabolism Brain - radiation effects Central nervous system Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges Fundamental and applied biological sciences. Psychology Imaging, Three-Dimensional - methods Imaging, Three-Dimensional - trends in situ NMR spectroscopy Magnetic Resonance Spectroscopy - methods Male Metabolomics - methods Metabolomics - trends microwave fixation Microwaves Neurology NMR Nuclear magnetic resonance Radionuclide Imaging rat brain Rats Rats, Sprague-Dawley Rodents Vertebrates: nervous system and sense organs |
| title | In situ 3D magnetic resonance metabolic imaging of microwave-irradiated rodent brain: a new tool for metabolomics research |
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